• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.22 no.11
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• pp.745-750
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• 2010

Since the implementation of the LMO Law in Korea, the importance of the design qualification of BSL3 lab. is emphasizing. In this study, multizone simulation for three kind of biohazard scenarios using CONTAM is performed for design qualification of BSL3 lab. Also, in the case of unexpected spread of contaminants such as Influenza A virus(H1N1) in BL3 zone, the design qualification is carried out for diffusion and decontamination of contaminants according to differential pressure of BSL3 anteroom and door area of BSL3 lab. Also, in this study, appropriateness of laboratory room differential pressure and air flow rate to maintain pressure difference between laboratory rooms, and energy consumption due to air change rate variation according to door area in BL3 lab. Simulation results show that these approach methods are used as a tool for the design and verification of BL3 lab.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.11
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• pp.813-822
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• 2012

In this study, the multizone simulation for biosafety of BSL3 lab. and energy simulation are carried out simultaneously by using linked model of CONTAM and TRNSYS. In BSL3 lab., annual energy consumption is approximately five to ten times more than the magnitude of the office building. This is because required air change rate is extremely large and it is difficult to maintain room pressure difference efficiently. To maintain pressure difference between laboratory rooms through sealing condition of doors and proper airflow control is significant. In this study, to predict indoor environment of the BSL3 lab.(Influenza A research lab.), the multizone simulation for four kinds of biohazard scenario is also performed as part of risk assessment. Multizone and energy simulation results by using linked model show that these approaches are used as a tool for the energy efficient design and operation method for the safer BSL3 lab. facilities.

• Journal of Life Science
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• v.18 no.11
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• pp.1543-1550
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• 2008

We studied the effects of smoking, which is one of indoor-environmental pollutants and related to various cancers, on glycoconjugates of rat sebaceous glands with the lectin histochemistry. To investigate the effects of smoking on glycoconjugates, male Sprague-Dawley rats were exposed to tabacco smoke for 10 minutes per day in an inhalation chamber for 1, 2, 3, and 5 days with active and passive exposure. For the structure of sebaceous glands we used PAS reaction, and for the glycoconjugates binding pattern 9 biotinylated lectins (DBA, SBA, PNA, BSL-1, WGA, RCA-1, UEA-1, Con A, and LCA) were used. Some remarkable changes, such as the decrease in the size of sebaceous glandular acini, the destruction of upper portion of sebaceous glands, vacuolation of central portion of sebocytes, and the immature sebaceous glandular acini were seen in the smoke-exposed rats. In the control rats, basal cells were stained with BSL-1, PNA and WGA, but the stronger reaction was founded in BSL-1 binding. Also, sebocytes were stained with PNA, WGA, Con A, BSL-1 and SBA, but stronger reactions were founded in PNA and Con A stainings. Specific changes in the lectin binding patterns were also observed in the smoke-exposed rats. In the basal cells of exposed rats, PNA binding increased, BSL-1 decreased but returned to control level, and WGA disappeared. Plus, immature glandular acini, which were not found in the control rats, were stained PNA, Con A and BSL-1, but the stronger reaction were founded in PNA and Con A binding. In conclusion, it was assumed that the tabacco smoke seriously effected on the structure and glycoconjugates metabolism of sebaceous glands.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.04b
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• pp.747-750
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• 1998

The application of spent liquor(BSL) obtained from alkaline sulfite-anthraquinone cooking of pine bark to cement mortar significantly improved the water-reducing ability and decreased the rate of cement hardening. However, the compressive and flexural strength of BSL addition to cement mortar were lower than that of PLAIN. The application of 0.2% antifoamer to BSL slightly decreased water-reducing ability, but remarkably improved the compressive and flexural strength of cement mortar. On the other hand, BSL decreased the rate of hardening of cement, which exhibited the protperties of the lignosulfonated based retarding water-reducing type. but the setting time of cement could be controlled by addition of Na2CO3 could be used to convert BSL to normal or accelerating concrete admixtures.

• Biomedical Science Letters
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• v.22 no.3
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• pp.65-74
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• 2016

The critical aspects of biosafety and bio-containment have been increasingly important in recent years. Biological agents involved in biological research projects at the Nanyang Technological University (NTU) Singapore are usually those with low risks. Biosafety level 2 or BSL 2 laboratories are widely used. However, biosafety measures which refer to the implementation of laboratory practices and procedures, specific construction features of laboratory facilities and safety equipment must be in place to reduce the exposure of laboratory personnel, the public or the environment to potentially infectious agents or other biological hazards. It is also required to pay more attention to laboratory-acquired infections (LAIs) which may occur in research laboratories, clinical laboratories or animal facilities. BSL 2 audit and certification program is implemented as an internal exercise covering laboratories in the university where biological agents are handled or biological research works are carried out. We have put some efforts to raise biosafety standards university-wide in both laboratory infrastructure and laboratory practices to a higher level. Common audit findings are briefly discussed in this presentation.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.2
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• pp.77-84
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• 2018

In Singapore, biosafety and biosecurity measures are controlled by the Biological Agents and Toxins Act (BATA) and other requirements by regulatory agencies. The law prohibits and otherwise regulates the possession, use, import, transhipment, transfer, and transportation of biological agents, inactivated biological agents, and toxins that are of public health concern. The law also defines the facility requirements for high risk biological agents and toxins. The containment facility (BSL 3) is a minimum requirement to handle biological agents that falls under Schedule 1 (Risk Group 3). The Nanyang Technological University School of Biological Sciences Biosafety Level 3 Facility (NTU-SBS BSL 3) was designed specifically for research involving potential hazardous biological materials. The facility requires yearly re-certification by an approved facility certifier to meet the local requirements and international biosafety standards for a containment facility in many instances. On the other hand, most NTU researchers conduct biological projects involving biological agents with low or moderate risk groups (Risk Groups 1 and 2 or biological agents described in schedule 3 and 4 of BATA) and GMOs, which need only a BSL 2 laboratory. BSL 2 laboratories are yet to be legally certified or registered in Singapore. Institutional Biosafety Committee (IBC) identifies the requirements; defines a minimum standard in the safe control of biological risks and registers all BSL 2 laboratories in the NTU. Therefore, under the guidance of the IBC, the University Biosafety and Biosecurity Programme includes the audit and certification program as a unique and an internal exercise to bring NTU biosafety to a higher level.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.25 no.10
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• pp.534-539
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• 2013

In general, entire supply air of the BSL3 laboratory should be vented to the outside for its biosafety and the air conditioning system should always be operating to maintain a room pressure difference. In this regard, annual energy consumption is approximately five or ten times greater than the magnitude of the office building. In addition, to adjust room pressure difference to the set value efficiently, the supply and exhaust duct system are installed in each room of the BSL3 lab. Thus, initial construction cost is extremely high. In this study, multizone simulation is performed to estimate maintaining the appropriate room pressure difference in the case of changing model A (each room supply and exhaust system) to model B (each zone supply and exhaust system) for verification of the BSL3 lab biosafety. Also, in the case of these two models, the multizone simulation for three kinds of biohazard scenario is performed as part of risk assessment. The analysis of initial construction cost of two models is conducted for comparison. According to the studies, initial construction cost of model B is less than about 22% of existing model A. Moreover, biosafety of the BSL3 lab is still maintaining in the case of the two models.

• The Journal of Industrial Distribution & Business
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• v.6 no.3
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• pp.9-17
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• 2015

Purpose - The study's aim is to assess performance quality delivered through a performance management system. The case study first measures the effectiveness of the present performance management system of Bhushan Steel Ltd. (BSL), and then secondarily analyzes the employee training impact. Research design, data, and methodology - A case study research approach using a survey was followed. The final sample size was 50, with a simple random sampling technique used. Primary data came from the company itself and the secondary from books, the Internet, journals, etc. Results - BSL employee appraisals are used for many developmental aspects such as competence in the present job, improvements, performance development, and training needs. The results indicate that individual performance can improve if employees perceive the system as more transparent (i.e., not so confidential). Conclusions - Overall, the BSL employees think that the company appraisal system is useful; however, to make it better the company has to improve many aspects including appraisals based on supervisor observations. The company could use a self-appraisal and a 360-appraisal approach to improve such assessments.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.1
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• pp.130-135
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• 2003

Mulberry, Mori folium leaves (ML) and fruits (MF), Lycii fructus (LF), and Panax ginseng have been known to maintain normal blood sugar levels (BSL) in folk medicine. Here we investigated the effects of coated rice with these herb extract to diabetic patients. Alloxan-induced diabetes mellitus (DB) mice (80 mg/kg BW., i.v.) separated into 3 groups. One group received the extract in high dose (6.5 mg/kg B.W.), and another one group received in low dose (1.3 mg/kg B.W.) perorally for 30 days. (+) control received no extracts. (-) control received neither of them. BSL, volume of water consumed (VWC), body weights (BW) of each group were measured and compared. BSL in both of the extracts-received groups got close to normal levels on 4th week after starting the experiments. VWC also showed the same pattern. BSL in (+) control remained high through the experiment periods. BW in (+) control remained high, but those in the extracts-received groups recovered to normal levels. The clinical trials for both of healthy volunteers and DB patients, conducted in a University Hospital, also showed that the rice coated with the extracts could control the BSL. From these results, we suggest that the functional rice with herb extracts could be used as a new alimentotherapy for DB patients.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.524.2-524
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• 1986

The Bacillus CMCase gene we have previously cloned in E. coli is contained in the 3.2 Kb chromosomal insert of the 7.5 Kb pBSl plasmid. We have also found that the CMCase produced by this gene has molecular weight of about 32,000 suggesting that the CMCase coding region lies on about 0.3 Kb fragment. The present report deals with a series of subclonings to localize more precisely the region coding for the CMCase production.