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First Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in Korea (옥시페탈룸에서 발생한 토마토반점위조바이러스 국내 첫 보고)

  • Eseul, Baek;Peter, Palukaitis;Ju-Yeon, Yoon
    • Research in Plant Disease
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    • v.28 no.4
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    • pp.231-236
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    • 2022
  • Oxypetalum coeruleum, commonly known as Tweedia, is a perennial herbaceous plant of the Apocynaceae family native to southern Brazil and Uruguay. Tweedia plants are grown as one of the most popular ornamental flowers for floral arrangement in Korea. In May 2021, several tweedia plants in a single greenhouse in Gimje, Jeollabuk-do were found to show virus-like symptoms including necrotic rings, vein-clearing, chlorotic mottle, and mosaic on the leaves, and necrosis on the stems. Here, we have identified tomato spotted wilt virus (TSWV) in symptomatic tweedia leaves by applying high-throughput RNA sequencing. In the result, a single infection by TSWV was verified without mixed infections of different virus species. To confirm the presence of TSWV, a reverse transcription polymerase chain reaction was performed with a specific primer set to the N gene of TSWV. The complete genomic sequence of L, M, and S segments of TSWV 'Oxy' isolate were determined and deposited in GenBank under accession numbers LC671525, LC671638, and LC671639, respectively. In the phylogenetic tree analysis by maximum likelihood method, 'Oxy' isolate showed a high relationship with TSWV 'Gumi' isolate from Gerbera jamesonii in Gyeongsangbuk-do, Korea; for all three RNA segments. To our knowledge, this is the first report of TSWV infection of O. coeruleum in Korea.

Whose Science is More Scientific? The Role of Science in WTO Trade Disputes

  • Kim, Inkyoung;Brazil, Steve
    • Analyses & Alternatives
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    • v.2 no.1
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    • pp.31-69
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    • 2018
  • This study examines the role of science in resolving trade disputes. After the Great East Japan Earthquake of 11 March 2011 that not only jeopardized the people of Japan, but also put the safety of an entire region at risk, the Republic of Korea (Korea) has imposed import bans as well as increased testing and certification requirements for radioactive material on Japanese food products. Japan has challenged these restrictions at the World Trade Organizations Dispute Settlement Body (DSB). This study aims to explain how international trade agreements and previous DSB rulings have dealt with different scientific viewpoints provided by confronting parties. In doing so, it will contrast the viewpoints espoused by Korean and Japanese representatives, and then analyzes the most similar case studies previously ruled on by the DSB, including the case of beef hormones and the case of genetically modified crops including biotech corn, both between the United States and the European Communities (EC). This study finds that science is largely subordinate to national interests in the case of state decision-making within the dispute settlement processes, and science has largely been relegated to a supportive role. Due to the ambiguity and lack of truly decisive decisions in the Appellate Body in science-based trade disputes, this study concludes that the Appellate Body avoids taking a firm scientific position in cases where science is still inconclusive in any capacity. Due to the panel's unwillingness to establish expert review boards as it has the power to do, instead favoring an individual-based system so that all viewpoints can be heard, it has also developed a system with its own unique weaknesses. Similar to any court of law in which each opposing party defends its own interests, each side brings whatever scientific evidence it can to defend its position, incentivizing them to disregard scientific conclusions unfavorable to their position. With so many questions that can arise, combined with the problems of evolving science, questions of risk, and social concerns in democratic society, it is no wonder that the panel views scientific information provided by the experts as secondary to the legal and procedural issues. Despite being ruled against the EC on legal issues in two previous cases, the EC essentially won both times because the panel did not address whether its science was correct or not. This failure to conclusively resolve a debate over whose science is more scientific enabled the EC to simply fix the procedural issues, while continuing to enforce trade restrictions based on their scientific evidence. Based on the analysis of the two cases of disputes, Korea may also find itself guilty of imposing an unwarranted moratorium on Japan's fish exports, only to subsequently pass new restrictions on labelling and certification requirements because Japan may have much scientific evidence at its disposal. However, Korea might be able to create enough uncertainty in the panel to force them to rule exclusively on the legal issues of the case. This will then equip Korea, like the EC in the past, with a way of working around the ruling, by changing whatever legal procedure they need to while maintaining some, if not most, of its restrictions when the panel fails to address its case on scientific grounds.

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Monitoring Pesticide Residues in Nuts and Seeds in Gyeonggi-do (경기도 내 유통 견과종실류의 잔류농약 실태 모니터링)

  • Yu-na Lee;Soo-Kyeong Moon;Jung-Hee Lee;Ae-ran Kim;Kyeong-Eun Moon;Choong-Won Kang;Jin-Hee Lee;Myoung-Ki Park
    • Journal of Food Hygiene and Safety
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    • v.38 no.3
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    • pp.152-157
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    • 2023
  • This study was aimed to investigate pesticides residue levels in nuts and seeds distributed in Gyeonggi-do. We investigated nuts and seeds in 131 products, which is pumpkin seeds (n=17), sunflower (n=12), sesame (n=23), perilla (n=11), peanut (n=18), almond (n=8), chestnut (n=6), walnut (n=14), pine nut (n=3), pecan (n=4), macadamia (n=3), brazil nut (n=5), cashew nut (n=4) and ginkgo nut (n=3). As a result of evaluating 339 pesticide residues, eight out of 131 samples were detected at the range of 0.01-0.07 mg/kg. Additionally, 7 out of 8 detected samples exceeded MRLs (Maximum Residue Limits) and 85% of them were imported. Pyraclostrobin applied on the positive list system (PLS) was detected in five Chinese pumpkin seeds samples, and the range was 0.02-0.04 mg/kg. In Indian and Korean sesame seeds, pendimethalin, boscalid, and bifenthrin were detected at 0.04, 0.05, and 0.06 mg/kg, respectively. The ratio of estimated daily intake (EDI) to acceptable daily intake (ADI) was 0.002-0.372%, indicating that the risk level was safe.

Does vitamin blends supplementation affect the animal performance, carcass traits, and nutrient digestibility of young Nellore finishing bulls?

  • Dhones Rodrigues de Andrade;Flavia Adriane de Sales Silva;Jardeson de Souza Pinheiro;Julia Travassos da Silva;Nathalia Veloso Tropia;Leticia Artuzo Godoi;Rizielly Saraiva Reis Vilela;Fernando Alerrandro Andrade Cidrini;Luciana Navajas Renno;Diego Zanetti;Tiago Sabella Acedo;Sebastiao de Campos Valadares Filho
    • Animal Bioscience
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    • v.36 no.12
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    • pp.1831-1841
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    • 2023
  • Objective: This study was realized to evaluate the effects of supplementation with blends of water and fat-soluble vitamins on animal performance and carcass traits of young Nellore bulls. Methods: Forty-three Nellore bulls, with an initial weight of 261±27.3 kg and a mean age of 8±1.0 months, were used. Five animals were slaughtered at the beginning of the experiment (reference group), to determine the initial empty body weight of the bulls that remained in the experiment. The remaining 38 bulls were fed ad libitum and distributed in a completely randomized design in a 2×2 factorial scheme, with or without supplementation of water-soluble (B-blend+ or B-blend-) and fat-soluble (ADE+ or ADE-) vitamin blends. Diets were isonitrogenous (120 g of crude protein/kg dry matter [DM] of total mixed ration) and consisted of a roughage:concentrate rate of 30:70 based on total DM of diet. The experiment lasted 170 days, with 30 days of adaptation and 140 days for data collection. At the beginning and end of the experimental period, the bulls were weighed to determine the average daily gain. To estimate the apparent digestibility of nutrients and microbial efficiency, spot collections of feces and urine were performed for five consecutive days. Results: DM, ashes, organic matter, crude protein, ethereal extract, neutral detergent fiber corrected for residual ash and residual nitrogenous, and N intake and apparent digestibility were not influenced by vitamin supplementation, but total digestible nutrients intake and non-fibrous carbohydrates digestibility were influenced by B complex vitamin supplementation. Nitrogen balance, microbial efficiency, and performance data were not influenced (p>0.05) by vitamin supplementation. Conclusion: Vitamin supplementation (a blend of water-soluble and fat-soluble vitamins or their combinations) does not influence the animal performance and carcass traits of young Nellore bulls.

A Study of Coloration of Topaz(I): Mineralogical and Chemical Study on the Topaz Selected from Some Localities of the World (토파즈의 人工着色 處理를 위한 硏究(I) : 世界 主要 産地別 토파즈의 鑛物學的 및 化學的 特性)

  • Han, Yi-Kyeong;Park, Maeng-Eon;Jang, Yong-Nam
    • Journal of the Mineralogical Society of Korea
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    • v.5 no.2
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    • pp.109-121
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    • 1992
  • For the purpose of mineralogical and chemical study on the topazes from various localities of world(Brzail, China, India, Nigeria and Sri Lanka), electron microprobe analysis(EPMA), neutron activation analysis(NAA), X-ray diffractometry, Raman spectroscopy, etch test, scanning electron microscopy, refractive index, specific gravity, fluid inclusion were performed. The chemical composition in topaz was discussed along with its physical and structural properties. Variations in the unit-cell dimension and physical properties of topaz were found to have a close relations in the unit-cell dimension and physical properties of topaz were found to have a close relationship with extent of substitution of $OH^-\;for\;F^-$. According to neutron activation analyses, the trace elements had no effects on the physical properties of topaz. Raman spectra showed that the peaks of topaz were different in intensity from one locality to another. Etching defects in topaz includes negative crystal defect o point-bottom pit(India, Nigeria) and net work defect of curl-bottom pit(Brazil, China). Fluid inclusions in topaz may be classiffied into liquid $CO_2$-bearing inclusion, gaseous inclusion, halite, sylvite-bearing inclusion and liquid inclusion. The results of this study can be useful to devising artificial coloring methods for topaz with different mineralogical compositions.

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A Study on Effect of B/L's Exemption Clauses Relating to the Governing Law of English Law (영국법의 준거법과 관련한 선하증권 면책약관의 효력에 관한 연구)

  • Han, Nak-Hyun;Jung, Jun-Sik
    • Journal of Korea Port Economic Association
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    • v.22 no.4
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    • pp.1-17
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    • 2006
  • In the Bill of Lading of The Irbenskiy Proliv is not subject to the Hague-Visby Rules in accordance with paragraphs (A) and/or (E) of cl.1 or to the Hague Rules in accordance with paragraphs (B) and/or (D) of cl.1. The Irbenskiy Proliv is very rare case that is effective to exempt the carrier as literal words of Bill of Lading. The action concerns cargoes of perishable goods shipped from Brazil to Japan, under Bills of Lading each of which contained an extensive carrier's exemption clause. A preliminary issue was ordered to be determined on the question whether c1.4 is effective to exempt the ralliers from any potential liability for the claims in this case. The court held that there is no reason to reject c1.4 as part of each of the contracts contained in or evidenced by the bills of lading; and it protects the carrier where damage to the goods shipped results from such causes. It is therefore effective to exempt the carriers from any potential liability for those claims.

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Clinical Features, Molecular Analysis, and Outcome of ERT in Korean Patients with Mucopolysaccharidosis Type VI (국내 6형 뮤코다당증의 임상 양상, 분자유전학적 특징 및 효소치료의 효과에 대한 고찰)

  • Wichajarn, Khunton;Kim, Jinsup;Yang, Aram;Sohn, Young Bae;Lee, Beom Hee;Yoo, Han-Wook;Cho, Sung Yoon;Jin, Dong-Kyu
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.16 no.1
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    • pp.24-33
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    • 2016
  • Mucopolysaccharidosis type VI (MPS VI) is a rare disease caused by the mutation of ARSB with prevalence range from 1/5,000 in northeast Brazil to 1/2,057,529 births in Czech Republic. In Asia, there is only one published figure in Taiwan of about 1/833,000 births. The exact prevalence in the Korean population is unknown, but we estimated the incidence of MPS VI is about 0.03/100,000 live births. Enzyme replacement therapy (ERT) with recombinant human Arylsulfatase B (rhASB) is a modality for the treatment of MPS VI that reduces the excretion of urine glycosaminoglycan (GAG) and improves joint motion, pulmonary function, and endurance. We presented the clinical features, molecular analysis and outcome of ERT in three Korean MPS VI patients. All patients had the typical characteristic clinical features of MPS IV. Short stature, dysostosis multiplex, corneal opacity and valvular heart disease were found at first presentation, while restrictive lung disease and carpal tunnel syndrome developed later in all patients. Molecular analysis demonstrated novel missense and nonsense mutation in the patients, including p.Ile 67Ser, p.Gly328Arg, $p.Arg191^*$, p.Asp352Asn, and p.Gly17Asp. After ERT, urine GAG was decreased in all patients. Skeletal involvement, corneal opacity, heart valve abnormalities and pulmonary function were not improved with ERT, but it had a better outcome on regarding joint motion and endurance. One patient underwent allogeneic bone marrow transplantation (BMT) prior to ERT, but their clinical response was not improved much after BMT. This study demonstrates clinical phenotypes and molecular analysis of the severe form of MPS VI in Korean patients.

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한국 표준시 제도의 타당성에 관한 연구

  • Hong, Seong-Gil;Ryu, Chan-Su
    • 한국데이터정보과학회:학술대회논문집
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    • 2002.06a
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    • pp.5-8
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    • 2002
  • 현재 우리 나라가 사용하고 있는 표준시는 I-시간이다. 이 표준시 사용에 대해 일부 이견이 있어, 우리 나라의 표준시 제도를 확립하기 위해 세계 각국의 표준시의 운용 상황을 분석 검토함으로서 우리나라는 현재 사용 중인 I-시간을 계속적으로 사용하는 것이 타당한 것으로 다음과 같은 결론을 얻을 수 있었다. 1) 세계에서 복수 표준시를 운용하고 있는 나라(러시아 등 9개국)는, 표준시 적용 지역을 태양시에 정확하게 맞추어 결정할 수 있을 것임에도, 3개국만 표준시가 태양시에 거의 일치할 뿐 나머지 6개국은 모두 태양시보다 표준시를 앞세워 적용하고 있다. 2) 세계 15대 대 영토국 중 단일표준시를 운용하고 있는 나라(중국 등 6개국) 중에서 3개국이 대체로 표준시가 태양시에 일치할 뿐이고, 나머지 3개국은 표준시를 태양시보다 $1{\sim}2$시간 이상이나 앞세워 적용하고 있다. 3) 전 세계의 145개 주요 단일 표준시 사용국 중 약 5%(중동의 지중해 연안국들과 동유럽의 일부 국가들) 정도에서 표준시를 태양시보다 30분 정도 늦춰 적용할 뿐이고, 태양시와 비슷하게 운용하는 나라는 약 56%, 표준시를 30분 이상 앞세워 적용하고 있는 나라는 약 39% 정도로서 대체로 태양시보다 빠른 표준시를 긍정적으로 받아들이고 있다. 4) 캐나다와 미국의 경우는 각 주에서 정한 표준시와는 다르게 운영하고 있는 지방들이 있는데, 이들 모두가 지리적인 위치로 보아 태양시보다 표준시가 어느 정도 빠르게 운영되도록 조정한 경우들이다. 5) 세계에서 UTC와 비 정수 시간차의 표준시를 운용하는 지역과 국가는 7개소 정도이다. 이들 중 표준시를 태양시에 맞추어 설정한 경우가 6개소이고, 지리적인 위치로 보아 표준시와 태양시가 일치할 수 있는데도 표준시를 30분 정도를 빠르게 운용하기 위해 조정한 경우가 1개소이다. 6) 세계에서 서머타임을 적용하고 있는 최저위도 국가는 북반구에서는 북회귀선에 걸쳐 있는 Mexico와 Cuba, 남반구에서는 Brazil의 남회귀선에 걸쳐 있는 남반부인데 이들은 모두 태양시와 일치하는 표준시를 운용하는 나라들이다. 한편 서머타임을 적용하고 있지 않는 최고위도 국가는 북반구에서는 Iceland, 남반구에서는 Argentina인데, 이 두 나라는 모두 이미 표준시를 태양시보다 1시간 이상 빠르게 적용하고 있는 나라들이다. 7) 복수 표준시간을 운용하고 있는 Canada와 미국에서는 국가적으로는 서머타임을 운용하고 있는데도 서머타임을 운용하고 있지 않는 주가 Canada에는 1개주, 미국에는 3개 주가 있는데, 이들은 모두 표준시가 태양시보다 30분${\sim}$1시간 정도 빠르게 적용되고 있는 곳이다. 8) 세계의 많은 나라가 표준시를 태양시보다 30분${\sim}$1시간 또는 그 이상까지도 빠르게 적용하고 있다는 사실, 그리고 세계의 많은 나라에서 서머타임을 시행하고 있다는 사실을 보면 태양시보다 표준시를 어느 정도 빠르게 적용해 생활하는 것은 인간 생체리듬에 악영향을 미치지 않으면서 오히려 시간 이용을 효율화하는 장점이 있는 것으로 판단된다. 9) 현재 우리나라는 경도 $135^{\circ}E$를 기준으로 하는 I-시간을 사용하고 있으면서 I-시간대역의 서쪽경계에 위치하여 태양시보다 표준시가 30분 정도 빠르다. 이는 한국인의 생체리듬에 결코 나쁜 영향을 주지 않으며, 오히려 시간을 효율적으로 사용할 수 있는 장점으로 작용하고 있는 것으로 판단된다. 아울러 여름철에 일광절약시간(서머타임)제의 필요성을 완화시켜줌으로서 표준시를 안정적으로 운용하는데 크게 기여하고 있다. 이는 표준시를 UTC와 정수시간차를 유지하면서 안정적으로 운영하려는 세계 각국의 표준시 운영 경향과도 일치하고 있다. 10) 우리나라가 사용하는 I-시간이 우리에게 이상과 같은 장점을 가지고 있음에도 불구하고 이 I-시간이 일제의 잔재라거나 또한 한국인의 생체리듬에 해롭다는 이유를 들어 우리 나라의 표준시를 경도 $127.5^{\circ}W$ 기준의 H'시간으로 변경시킨다면UTC와의 8시간 30분차 시간을 운영하게 됨으로서 국제화 시대를 사는데 큰 불편을 초래할 뿐만 아니라, 추가적으로 서머타임제를 도입하게 되는 등 커다란 부작용을 겪게 될 것이다.

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Mineralogical Studies of the Tourmaline for Medicinal Applications by Production Localities (본초 광물로서의 활용을 위한 산지별 전기석의 광물학적 연구)

  • Jie, Yan;Kim, Seon-ok;Park, Hee Yul;Park, Maeng-Eon
    • Economic and Environmental Geology
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    • v.51 no.4
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    • pp.345-358
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    • 2018
  • In this study, we have performed electron probe micro analyzer (EPMA), X-ray differaction (XRD), inductively coupled plasma spectroscopy (ICP), Fourier transform Raman spectroscopy (FT-Raman), far-infrared (FIR), nuclear magnetic resonance (NMR), and pH-DO Analyses for characterizing medicinal mineralogy aspect of the black tourmaline (Shantung, china), black and pink tourmaline (Minas Geraris, Brazil), black touemaline (Daeyu mine, Korea). In addition, heating effects of the tourmaline sauna as well as the effects of tourmaline powder-added soap on skin troubles have been investigated. It has been revealed that chemical composition of the tourmaline is either high in Fe-, Al-, B-rich types. Ratio of the K-Ca, Na-K, and Fe-B reflects the component change property of solid solution. $CaO/CaO+Na_2O$ and MgO/FeO+MgO ratio show high positive correlation. When tourmaline reacts with distilled water, extended reaction time DO values approximately decrease and it stabilizes at DO = 10. Otherwise, pH values increase until 6 hours and it stabilizes at pH = 8 after 24 hours. Distilled water changes to alkaline when it reacts with tourmaline powder and particles. Tourmaline showed lower absorption spectrum strength and transmittance at short wave, where absorption spectrum wavelength and strength were determined by the content of the composition elements and characteristics of crystallography. Increase of the Fe content has been confirmed to be the cause for the reduction of irradiation. For the chemical composition and spectral property of the tourmaline particle samples, it has been found that Si and Fe contents show positive correlation with Far-Infrared irradiation, while Al and Mg contents show negative correlation. For tourmaline powder, it has been confirmed that $^{17}O-NMR$ FWHM (full width at half maximum) decreases when reacts with distilled water. Tourmaline sauna (approximately $100^{\circ}C$) was found to increase $0.5-1.5^{\circ}C$ of body temperature, average of 12 heartbeat, and 10mg Hg of blood pressure. Tourmaline soap had very good aesthetic effect to skin and was confirmed to have above the average improvements to skin troubles (e.g., allergy or atopy).

Chemical and Optical Absorption Spectroscopic Study of Colored Tourmalines (유색 전기석의 화학적 및 광학흡수 분광학적 연구)

  • Kim, Hee-Jong;Kim, Soo-Jin
    • Journal of the Mineralogical Society of Korea
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    • v.6 no.1
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    • pp.1-16
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    • 1993
  • The chemical and optical absorption spectroscopic characters of pink and colorless tourmalines from San Diego mine in California, U.S.A., blue/green tourmalines from anonymous mine, Brazil, and brownis black tourmalines from Uncheon and Haksan mines in Korea have been studied using X-ray diffractometer, electron microprobe, optical absorption spectroscopy, and heat treatment. Least-squares refinements give unit cell diminsions : a = 15.96-16.01 ${\AA}$, c = 7.15-7.16 ${\AA}$ for the brownish black tourmalines, a = 15.82 - 15.87 ${\AA}$, c = 7.09 - 7.10 ${\AA}$ for pink tourmalines, and a = 15.88 - 15.94 ${\AA}$, c = 7.12 - 7.15 ${\AA}$ for blue green tourmalines. The colors of tourmalines are responsible for the transition elements. The pink color is attributed to the $Mn^{3+}$ ions, the blue-green to $Fe^{2+}$ and $Mn^{2+}$, bluish green to $Cu^{2+}$, and the brownish black to $Fe^{2+}$, $Fe^{2+}$ - $Fe^{3+}$, and $Fe^{2+}$ - $Ti^{4+}$. The $Mn^{3+}$ ions of pink color tourmalines are stabilized in the Y sites compressed along the O(1)H-O(3)H axis by Jahn-Teller distortion. Heating removes the pink or red component from tourmalines, producing the colorless stones from the pink and red ones. The bluish green samples change into the greenish blue ones and a certain yellowish green samples change into the light green ones by heat treatment. In the elbaite-schorl series, the concentration of Fe and Mn are variable depending on the color zones. The green zone is characterrized by the high content of Fe and Mn are variable depending on the color zones. The green zone is characterized by the high content of Fe, whereas the pink zone by the high content of Mn. Mn increases in deep yellow zone compared with yellow or colorless zones.

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