• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2231-2234
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• 2007

A new BODIPY derivative bearing piperazine group was synthesized and its fluorescent changes towards metal ions as well as pH are studied. The title compound displayed a moderate selectivity for Hg2+ among the metal ions examined.

• Bulletin of the Korean Chemical Society
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• 제29권9호
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• pp.1831-1834
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• 2008

• Bulletin of the Korean Chemical Society
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• 제31권2호
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• pp.507-510
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• 2010

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1247-1250
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• 2014

• 한국발생생물학회지:발생과생식
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• 제14권4호
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• pp.297-306
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• 2010

내분비교란물질로 알려진 Tributyltin(TBT)는 흰쥐 정소 내 생식세포와 간질세포의 세포자연사를 일으켜 정소의 기능을 감소시키는 것으로 보고되고 있으나, 그 기전은 명확히 밝혀져 있지 않다. 따라서 본 연구에서는 정소 내 간질세포를 표적으로 TBT에 의해 지방세포로 분화가 유도되는 지를 확인하고, 이로 인한 정소 내 세포자연사와의 연관성을 알아보고자 하였다. 3주령 된 수컷 흰쥐에 각각 TBT 1 mg과 10 mg/kg/day을 1주일 동안 경구 투여한 후 정소를 분리하여 무게를 측정하였다. 획득된 정소의 일부는 냉동 절편을 만들어 BODIPY로 지방세포를 염색하였고, 일부는 파라핀 절편을 만들어 TUNEL 염색을 수행하였다. 나머지 정소는 정소 백색막을 제거한 후 세정관 사이에 존재하는 간질세포를 분리하였다. 분리된 간질세포에서 total RNA를 추출한 다음 real-time PCR 방법으로 지방세포 유도 유전자들과 세포자연사 관련 유전자들을 분석하였다. 정소의 무게는 대조군에 비교해 TBT 10 mg을 투여한 군에서 유의하게 감소하였다. BODIPY 염색 결과, TBT 10 mg을 투여한 군의 간질세포에서 염색된 세포의 수가 증가하였고, TUNEL 염색 결과에서도 대조군과 비교해 TBT 투여한 군에서 간질세포 내 세포자연사가 증가하는 것을 확인할 수 있었다. Real-time PCR을 이용해 간질세포 내 유전자 발현을 분석한 결과, 투여된 TBT의 농도가 증가할수록 PPAR${\gamma}$, aP2, PLIN, PGAR 등 지방세포 유도 유전자들의 발현이 증가하였고, 이와 함께 TNFRSF1A, TNFSF10과 같은 세포자연사 관련 유전자의 발현도 증가하는 것을 확인할 수 있었다. 이상의 결과에서 환경성 내분비 교란물질로 알려진 TBT에 노출될 경우 정소 내 간질세포의 지방세포로의 분화가 유발되면서 세포자연사가 증가하고, 이에 따른 정소 기능의 저하를 야기시킬 수 있는 것으로 사료된다.

• 한국광과학회:학술대회논문집
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• 한국광과학회 1999년도 학술대회지
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• pp.58-59
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• 1999

• 한국수정란이식학회지
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• 제26권1호
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• pp.71-78
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• 2011

This study was undertaken to find out the effect of cholesterol and serum albumin on sperm ability and lipid peroxidation levels period to the liquid storage of miniature pig sperm. Ejaculated semen from miniature pigs was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle, and extended with Modena solution {with and without BSA, methyl-beta-cyclodextrin (-cholesterol) and cholesterol loaded cyclodextrin (+cholesterol)}. Each semen was assessed for viability (SYBR-14/PI staining) and acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining at 1, 3, 5, 7 and 10 days of storage. At for the effects of cholesterol and serum albumin on lipid peroxidation, semen were incubated with $H_2O_2$ ($10\;{\mu}M$), and lipid peroxidation level were measured by flow cytometry using the lipid peroxidation reporter probe $C_{11}-BODIPY^{581/591}$. The result, lipid peroxidation level in sperm added with cholesterol were lower in $10\;{\mu}M$ $H_2O_2$ compared to the added sperm with serum albumin. Also, added cholesterol to sperm had significant (p<0.05) higher viability when storage for 7 and 10 days and lower when 10 days of storage percentage of acrosome-reacted sperm (AR pattern) in acrosome state as say result compared to other treated groups. In conclusion, role of cholesterol during lipid storage in miniature pig spermatozoa was protected boar spermatozoa from lipid peroxidation prior to lipid storage. Addition serum albumin during lipid storage in sperm may be induce sperm membrane damage by lipid peroxidation. Therefore, addition of cholesterol to miniature pig sperm will be lead to extension of liquid storage periods.

• 동의생리병리학회지
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• 제26권4호
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• pp.455-461
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• 2012

Obesity is associated with numerous diseases such as type 2 diabetes, hypertension and cancer. Inhibition of adipogenesis is a effectite strategy to anti-obesity. In this study, Galla Chinenisis extract (GCE) inhibited adipocyte differentiation in OP9 cells. There was no cytotoxicity when cells were treated with GCE in designated time intervals, unaffected by concentration. In this cell model, increases in fat storage were inhibited by 2 days treatment with various concentration of GCE, visualized by Oil red-O, BODIPY and DAPI staining. To understand the underlying mechanism at the molecular level, the effects of GCE were examined on the expression of the genes involved in adipogenesis by real-time PCR. In the progress of adipocyte differentiation with GCE-treated, the mRNA level of adipogenic genes such as peroxisome-proliferator-activated receptors gamma ($PPAR{\gamma}$), computer-assisted axial tomography/enhancer binding protein-alpha ($C/EBP{\alpha}$) were decreased. Also, GCE treatment inhibited increase of mRNA expression, which is adipogenic factor such as fatty acid synthase (FAS), hormone-sensitve lipase (HSL), lipoprotein lipase (LPL), and adipocyte-specific lipid binding protein (aP2). Therefore, the result of this study suggest that Galla Chinenisis extract can prevent adipocyte differentiation and GCE may have a great potential as a novel anti-adipogenic agent.

• Nutritional Sciences
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• 제9권1호
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• pp.48-54
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• 2006

Methods have been developed to evaluate the total antioxidant capacity of foods and plasma but limitations are associated with their ability to determine precisely the contribution of lipophilic antioxidants in a lipid milieu as well as interactions among them Thus, we modified the Oxygen Radical Absorbance Capacity (ORAC) assay to determine the peroxyradical scavenging ability of both hydrophilic and lipophilic compartments in plasma The hydrophilic ORAC assay was performed in a phosphate buffer system utilizing 2,2'-azobis (2-amidinopropane) dihydrochloride as a peroxyradical generator and fluorescein as the target The lipophilic ORAC assay was carried out in a dimethylsulfoxide :butyronitrile (DMSO/BN, 9:1 v/v) system using 2,2'-azobis (2,4-dimethyl valeronitrile) as a peroxyradical generator and BODIPY C11 581/591 as the target Analyses were conducted in bovine serum supplemented with water - and lipid - soluble antioxidants and in human plasma. Albumin (0.5$\sim$5 g/dL) and uric acid (0.1$\sim$0.5 $\mu$mol/L) increased hydrophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.97 and 0.98, respectively) but had no impact on lipophilic ORAC values. $\alpha$-Tocopherol (15$\sim$200 $\mu$mol/L) increased lipophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.94); neither $\alpha$-tocopherol nor $\beta$-carotene had an impact on hydrophilic ORAC values. However, addition of $\beta$-carotene at physiological concentration (0.23$\sim$1.86 $\mu$mol/L), either alone or in combination with other carotenoids, had no significant impact on lipophilic ORAC values. Thus, while assays of 'total antioxidant capacity' in biological matrices would be a useful research and clinical tool, existing methods are limited by the lack of complete responsiveness to the full range of dietary antioxidants.

• 한국임상수의학회지
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• 제33권6호
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• pp.356-361
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• 2016

During storage, boar spermatozoa undergo several changes including diminished motility and viability and accumulated reactive oxygen species (ROS). In this study, we investigated the effects of green tea extract (GTE) supplementation in the Sui Dil extender on the sperm motility, viability, ROS and lipid peroxidation (LPO) of long-term preserved boar semen at $17^{\circ}C$. A total number of eight boars were used for this experiment. Pooled ejaculates were diluted to $20{\times}10^6sperm/ml$ in the Sui Dil extender containing 0 (control), 1, 10, 100 or 500 mg/l GTE and were preserved at $17^{\circ}C$ for 24, 72, 120 and 168 h, respectively. At each storage time, sperm motility and viability were estimated by microscopic examination and the fluorescent double stain $Fertilight^{(R)}$, respectively. Sperm ROS level and LPO were assessed using the 2', 7'-dichlorodihydrofluorescein diacetate ($H_2DCFDA$)/propidium iodide (PI) and C11-BODIPY581/591/PI with flow cytometry, respectively. Compared to that of the 500 mg group, there were higher sperm motility and viability in the 1, 10 and 100 mg GTE groups during the preservation from 24 to 168 h (p < 0.05). The ROS levels of the 10 and 100 mg groups during the 168 h preservation were lower than those of the 0, 1 and 500 mg groups (p < 0.05). There were no significant differences in LPO regardless of the preservation period or the GTE concentration. In conclusion, the optimal concentrations (10 and 100 mg/l) of GTE that led to lower ROS levels may be useful for liquid boar sperm preservation at $17^{\circ}C$ for a period of 168 h.