• The Korean Journal of Physiology
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• 제20권2호
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• pp.225-235
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• 1986

가토 근위세뇨관에서 Percoll gradient에 의해 분리한 기저막소포(basolateral membrane vesicle)에서 PAH 이동에 미치는 여러 음이온들의 영향을 rapid filtration technique을 이용하여 관찰하였다. NA-dependent PAH 축적은 용액내 $SO_4$와 $SSO_3$에 의하여 유의하게 억제되었으나 $Cl,\;SCN,\;HPO_4,\;acetate$ 및 oxalate에 의해서는 영향을 받지 않았다. 용액내의 $SO_4$는 상경적으로 PHA 이동을 억제하였다. 소포내 음이온을 부하시킨 후 PAH 이동을 측정했을 때 $acetate,\;SO_4$ 및 PAH의 부하에 의해 PAH 이동은 유의하게 증가되었으며, 소포내 부하되는 $SO_4$농도가 증가함에 따라 PAH 축적은 증가되었다. $SO_4$에 의한 PAH의 trans-stimulation은 Na 농도 경사 존재시 더욱 증가되었으며 이들은 2 mM probenecid 및 1mM SITS에 의해 억제되었다. 이들 결과들은 신피질세뇨관 세포의 기저막에 PAH/음이온 교환에 의해 PAH가 이동되는 기전이 존재한다는 것을 가르킨다. 그러나 PAH 이동에 영향이 없는 음이온들은 PAH/음이온 교환에 기질로써 작용하지 않기 때문인지 아니면 이들 음이온들의 높은 투과성에 인해 나타난 결과인지는 더욱 추구해 보아야 확인될 것으로 생각된다.

• IMMUNE NETWORK
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• 제15권3호
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• pp.142-149
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• 2015

Lung fibrosis is a life-threatening disease caused by overt or insidious inflammatory responses. However, the mechanism of tissue injury-induced inflammation and subsequent fibrogenesis remains unclear. Recently, we and other groups reported that Th17 responses play a role in amplification of the inflammatory phase in a murine model induced by bleomycin (BLM). Osteopontin (OPN) is a cytokine and extracellular-matrix-associated signaling molecule. However, whether tissue injury causes inflammation and consequent fibrosis through OPN should be determined. In this study, we observed that BLM-induced lung inflammation and subsequent fibrosis was ameliorated in OPNdeficient mice. OPN was expressed ubiquitously in the lung parenchymal and bone-marrow-derived components and OPN from both components contributed to pathogenesis following BLM intratracheal instillation. Th17 differentiation of $CD4^+$ ${\alpha}{\beta}$ T cells and IL-17-producing ${\gamma}{\delta}$ T cells was significantly reduced in OPN-deficient mice compared to WT mice. In addition, Th1 differentiation of $CD4^+$ ${\alpha}{\beta}$ T cells and the percentage of IFN-$\gamma$-producing ${\gamma}{\delta}$ T cells increased. T helper cell differentiation in vitro revealed that OPN was preferentially upregulated in $CD4^+$ T cells under Th17 differentiation conditions. OPN expressed in both parenchymal and bone marrow cell components and contributed to BLM-induced lung inflammation and fibrosis by affecting the ratio of pathogenic IL-17/protective IFN-$\gamma$ T cells.

• 대한화학회지
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• 제42권4호
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• pp.443-448
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• 1998

용액열량계를 이용하여, 25$^{\circ}C$ 메탄올 용액에서 dibenzo-16-crown-5(DB16C5)의 유도체들과 금속이온과의 착물형성에 관한 열역학적인 파라미터인 log K, ${\Delta}H$, $T{\Delta}S$를 결정하고, dibenzo-16-crown-5의 유도체들을 운반체로 사용하여 벌크액체막과(bulk liquid membrane, BLM) 지지액체막(supported liquid membrane, SLM) 실험을 통하여 금속이온의 이동현상을 관찰하였다. BLM과 SLM에서 운반체로 DB16C5유도체를 사용했을 때 $Ag^+$ 이온이 많이 이동되었고 다른 금속이온들은 약간 이동된 것으로 관찰되었다. 액체막을 통한 그속이온의 이동에서 중요한 파라미터로 작용하는 인자는 리간드의 구조, 곁가지의 길이, 주개원자의 수와 종류, 안정도상수, 리간드의 농도 등을 들 수 있다.

• 한국환경성돌연변이발암원학회지
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• 제9권1호
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• pp.23-32
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• 1989

Chinese hamster ovary (CHO)-K1 cells echibited a differential sensitivity in the process of DNA repair synthesis induced by ethyl methanesulfonate (EMS) or bleomycin (BLM) in relation to cell cycle. Two assays were employed in this study: alkaline elution and unscheduled DNA synthesis. The post-treat-ment with aphidicolin (APC), an inhibitor of DNA polymerase alpha, inhibited DNA repair synthesis induced by EMS in G2 phase, while APC did not show any effect on BLM-induced DNA repair synthesis in all phases. On the other hands, the 2', 3'-dideoxythymidine (ddTTP), an inhibitor of DNA polymerase beta, inhibited DNA repair synthesis induced by EMS or BLM in both of G1 and G2 phases. These results suggested that the involvement of DNA polymerase alpha and beta in DNA repair was dependent on cell stage or used chemical agent.

• 한국운동역학회지
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• 제23권2호
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• pp.149-157
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• 2013

In the present study, we aimed to elucidate how muscle strength and activity are affected by movement pattern(bilateral [BLM] & unilateral movement [ULM]) and movement velocity($0^{\circ}$/s, $60^{\circ}$/s, $120^{\circ}$/s) at maximum effort, and to elucidate the relationship between a left/right asymmetry and bilateral deficit. A total of 18 healthy males participated in the study. Each participant performed maximum knee extension bilaterally and unilaterally while the EMG and moment were recorded, and then the relationships between the asymmetry and bilateral deficit were analyzed. The peak moments for the isokinetic motion at $60^{\circ}$/s and $120^{\circ}$/s and overall muscle activities of lower extremity were significantly reduced for the BLM in comparison to the ULM. And though the asymmetry in ULM were maintained during BLM at all velocities, the bilateral deficits at the velocity of $0^{\circ}\acute{y}$/s and $120^{\circ}\acute{y}$/s were significantly correlated with increased asymmetries of muscle strength in ULM. In conclusion, the reduction in the muscle strength exhibited in bilateral knee extension was shown to arise partially from a reduction in muscle activity, and left/right asymmetry was found to be associated with mechanical reduction in bilateral movement. These findings suggest that training aimed at increasing muscle strength must involve methods and strategies intended to reduce left/right asymmetry.

• Toxicological Research
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• 제24권4호
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• pp.273-280
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• 2008

The single intratracheal instillation (ITI) of bleomycin (BLM) is a widely used method for inducing experimental pulmonary fibrosis in rat model. In the present study, pulmonary function tests (PFTs) of tidal volume ($V_T$), minute volume ($V_M$), and respiratory frequency ($F_R$) have been applied to study their possibility as a tool to monitor the progress of BLM-induced lung injury in rat model. Rats were treated with a single ITI of BLM (2.5 mg/kg) or saline (control). Animals were euthanized at 3, 7, 14, 21, and 28 days post-ITI. Lung toxicity effects were evaluated by inflammatory cell count, lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage fluid (BALF), and light microscopic examination of lung injury. The PFT parameters were measured immediately before the animals were sacrificed. BLM treatment induced significant cellular changes in BALF-increase in number of total cells, neutrophils, and lymphocytes along with sustained increase in number of macrophages compared to the controls at days 3, 7, and 14. BALF LDH level was significantly increased compared to that in the controls up to day 14. On day 3, infiltration of neutrophils was observed in the alveolar spaces. These changes developed into marked peribronchiolar and interstitial infiltration by inflammatory cells, and extensive thickening of the interalveolar septa on day 7. At 14, 21, and 28 days, mild peribronchiolar fibrosis was observed along with inflammatory cell infiltration. The results of PFT show significant consistencies compared to the results of other toxicity tests. These data demonstrate that the most suitable time point for assessing lung fibrosis in this model is 14 days post-ITI of BLM based on the observation of fibrosis at 14, 21, and 28 days. Further, the progress of lung injury can be traced by monitoring the PFT parameters of $F_R$, $V_T$, and $V_M$.

• 대한위생학회:학술대회논문집
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• 대한위생학회 2007년도 춘계학술심포지움
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• pp.97-117
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• 2007

• Toxicological Research
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• 제11권1호
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• pp.103-108
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• 1995

Rat renal proximal tubule suspension was prepared from adult male Sprague Dawley rat (250-300g) by mechanical (non-enzymatical) method and evaluated as a pontential model for mechanistic studies and early screening of nephrotoxicity, using anionic antibiotics (cephaloridine). Cephaloridine (CPL) produced an increase in LDH release into media. This release results from decrease a proximal tubule cell viability and subsequently increase the permeability of cell viability and subsequently increase the permeability of cell membrane. Since loss of intracellular potassium and ATP into media is the sign of disruption of cell membrane, especially basolateral membrane (BLM), CPL induced proximal tubule cell compromise also appear be associated with BLM, maybe $Na^+-K^+$ ATPase. Also seen was significant depression in brush border membrane (BBM) ALP activity and no significantly increase in BBM GGT activities. The inhibition of typical anion, PAH accumulation (especially, CPL 5 mM) and cation, TEA (especially, 4hours incubation) were seen dose dependently. This is because of CPL accumulation in renal proximal tubule and increase of cytotoxicity.

• 한국지하수토양환경학회지:지하수토양환경
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• 제22권1호
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• pp.49-58
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• 2017

Biotic ligand model (BLM) and species sensitivity distribution (SSD) were used to determine the site-specific Cu threshold concentration (5% hazardous concentration; HC5) in soil pore water. Model parameters for Cu-BLM were collected for six plants, one collembola, and two earthworms from published literatures. Half maximal effective concentration ($EC_{50}\{Cu^{2+}\}$), expressed as $Cu^{2+}$ activity, was calculated based on activities of major cations and the collected Cu-BLM parameters. The $EC_{50}\{Cu^{2+}\}$ varied from 2 nM to $251{\mu}M$ according to the variation in environmental factors of soil pore water (pH, major cation/anion concentrations) and the type of species. Hazardous activity for 5% (HA5) and HC5 calculated from SSD varied from 0.076 to $0.4{\mu}g/L$ and 0.4 to $83.4{\mu}g/L$, respectively. HA5 and HC5 significantly decreased with the increase in pH in the region with pH less than 7 due to the decrease in competition with $H^+$ and $Cu^{2+}$. In the region with pH more than 7, HC5 increased with the increase in pH due to the formation of complexes of Cu with inorganic ligands. In the presence of dissolved organic carbon (DOC), Cu and DOC form a complex, which decreases $Cu^{2+}$ activity in soil pore water, resulting in up to 292-fold increase in HC5 from 0.48 to $140{\mu}g/L$.

• 사회복지연구
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• 제44권3호
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• pp.421-450
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• 2013

본 연구의 목적은 급여제한 사전통지제도가 건강보험료 체납자의 체납보험료 자진납부에 미치는 영향을 실증적으로 파악하는 것이다. 본 연구에서는 건강보험공단의 전산자료부터 2010년 1월 21일에 실시된 제 8차 급여제한 사전통지 대상자 155만 명에 관한 자료를 표본으로 추출한 다음 표본을 체납자집단(사전통지 도달, 사전통지 미도달)과 비체납자집단(비대상 체납자, 차상위)로 분류하고 각 집단으로부터 체납자 규모의 10%에 해당하는 15만 명씩을 최종표본으로 추출하여 분석자료로 사용하였다. 다양한 로지스틱회귀분석모형을 추정한 결과, 급여제한 사전통지제도가 통계적으로 유의한 수준인 체납보험료 납부독려 효과를 가진 것으로 나타났다. 그러나 분석결과의 통계적 유의성과는 별개로 급여제한 사전통지제도의 효과는 매우 제한적인 것으로 나타났다. 연구대상 체납자 중 급여제한 사전통지를 받은 이후 체납보험료의 일부 또는 전부를 납부한 체납자는 직역 차이를 고려하더라도 25% 미만 수준에 불과한 것으로 밝혀졌다. 이처럼 체납보험료 납부율이 저조한 이유는 보험료 체납자 중 상당수가 빈곤으로 인해 보험료 납부능력이 없는 생계형체납자이기 때문이라 판단된다. 이러한 현실을 고려할 때, 건강보험공단이 실시하고 있는 급여제한 사전통지제도나 자진납부기간 운영 등은 일부 체납자에게는 보험료 납부독려 효과가 있으나 대다수의 보험료 체납자들에게는 효과가 없으며 오히려 보험료 체납자의 비용부담을 가중시켜 체납 상황에서 벗어나기 어렵게 만드는 결과를 가져올 수 있다. 본 연구에서는 이러한 연구결과를 바탕으로 급여제한 사전통지제도의 개선을 위한 일련의 정책방안을 제시하였다.