• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.4
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• pp.275-280
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• 2004

In this study, the effcts of kelp (Ascophyllum nodosum) meal on growth and immune responses of parrot fish (Oplegnathus fasciatus) were studied. Fish were fed an experimental diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal in a controled diet. Several factors such as weight gain, hematological parameters and nonspecific immune responses were evaluated far 0, 2, 4 and 8 weeks after the administration of the kelp meal supplemented diet. Weight gain in the fish fed the diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal was not significant among the tested groups. The NBT reaction of the phagocytes in the head kidney and the phagocytic rate/index of phagocytes in the peripheral blood were significantly higher than the control group. But, there was no significant difference both in complement bactericidal activity, mucus Iysozyme activity and hematology among each group.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.5
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• pp.97-102
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• 2018

Combat vehicles require high levels of maneuverability, firepower, armor, and operability. A high-performance power system is required for optimal maneuverability. The fuel pump which supplies fuel stably is very important to achieve this. The fuel pump consists of a pump part, a motor part, and a control part. It is equipped with a BLDC motor. Numerous failures of the fuel pump occurred during vehicle operation when exposed to vibration, shock, and high temperature. The cause of failure was confirmed to be stator slip of the BLDC motor. Stator slip is a consequence of the interference loss between the stator and the housing of the motor part in an high temperature environment. The failure of the fuel pump was solved through size control of the motor housing and the stator. We performed vibration testing at high temperature for verification. This study contributes to improving the reliability of combat vehicles.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1818-1825
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• 2007

S-Adenosylmethionine (SAM) was previously documented to activate secondary metabolism in a variety of Streptomyces spp. and to promote actinorhodin (ACT) and undecylprodigiosin (RED) in Streptomyces coelicolor. The SAM-induced proteins in S. coelicolor include several ABC transporter components (SCO5260 and SCO5477) including BldKB, the component of a well-known regulatory factor for differentiations. In order to assess the role of these ABC transporter complexes in differentiation of Streptomyces, SCO5260 and SCO5476, the first genes from the cognate complex clusters, were individually inactivated by gene replacement. Inactivation of either SCO5260 or SCO5476 led to impaired sporulation on agar medium, with the more drastic defect in the SCO5260 null mutant (${\Delta}SCO5260$). ${\Delta}SCO5260$ displayed growth retardation and reduced yields of ACT and RED in liquid cultures. In addition, SAM supplementation failed in promoting the production of ACT and RED in ${\Delta}SCO5260$. Inactivation of SCO5476 gave no significant change in growth and production of ACT and RED, but impaired the promoting effect of SAM on ACT production without interfering with the effect on RED production. The present study suggests that SAM induces several ABC transporters to modulate secondary metabolism and morphological development in S. coelicolor.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.12
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• pp.1725-1728
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• 2004

An experiment was conducted to investigate the efficiency of transfer of dietary iron sources to eggs of laying hens. Eighty ISA-Brown laying birds of 30 wk old were housed in 40 cages of 2 birds each. Eight birds in four cages were assigned to one of the following ten treatments: T1; control, T2; 100 ppm iron supplementation with iron-methionine chelate (Fe-Met-100), T3; Fe-Met- 200, T4; Fe-Met-300, T5; 100 ppm iron supplementation with iron sulfate ($FeSO_4$-100), T6; $FeSO_4$-200, T7; $FeSO_4$-300, T8; 100 ppm iron supplementation with Availa-$Fe^{(R)}$ (Availa-Fe-100), T9; Availa-Fe-200 and T10; Availa-Fe-300. Results of 40 d feeding trial showed that there were no consistent responses in laying performance by source and level of iron supplementation. However, eggshell strength and color were improved by Fe supplementation. Egg iron content was maximized at 10-15 days after feeding supplemental Fe. Fe- Met was the most effective source in enriching Fe of eggs followed by Availa-Fe and $FeSO_4$. Increasing supplementary Fe level more than 100 ppm was not effective in Fe-Met and Availa-Fe treatments. Average Fe enrichment of 18% was achieved after feeding Fe-Met-100 for 15 d. In conclusion, enrichment of Fe in egg could be effectively achieved by supplementation of Fe-Met-100 for 15 d.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.305-312
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• 2007

This study focused on the involvement of the unusual nucleotide (p)ppGpp, a stringent factor, during the morphological and physiological differentiation of Streptomyces coelicolor. Two genes, relA and rshA, were disrupted to demonstrate the roles of the stringent factor in the differentiation. The intracellular concentration of (p)ppGpp in the wild-type (M600) and disrupted mutants was measured in relation to the intentional starvation of a specific nutrient, such as carbon, nitrogen, and phosphate or the in situ depletion of nutrients in a batch culture. As a result, it was found that the morphological characteristic of the ${\Delta}relA$ mutant was a bld phenotype forming condensed mycelia, whereas the ${\Delta}rshA$ mutant grew fast-forming spores and straightforward mycelia. In both mutants, the production of actinorhodin (Act) was completely abolished, yet the undecylprodigiosin (Red) production was increased. Intracellular (p)ppGpp was detected in the ${\Delta}relA$ mutant in the case of limited phosphate, yet not with limited carbon or nitrogen sources. In contrast, (p)ppGpp was produced in the ${\Delta}rshA$ mutant under limited carbon and nitrogen conditions. Therefore, (p)ppGpp in S. coelicolor was found to be selectively regulated by either the RelA or RshA protein, which was differentially expressed in response to the specific nutrient limitation. These results were also supported by the in situ ppGpp production during a batch culture. Furthermore, it is suggested that RelA and RshA are bifunctional proteins that possess the ability to both synthesize and hydrolyze (p)ppGpp.

• Journal of Power System Engineering
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• v.18 no.4
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• pp.43-51
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• 2014

In this paper, transient temperature and vibration characteristics of a brushless DC (BLD(c) motor are studied for external load (165W~495W) and rotational speed (2000 rpm~4000 rpm). For experiment, a simple measurement system is developed to allow a change in load and speed for measuring transient temperature and vibration simultaneously. Temperature and vibration were also measured under the conditions of natural convection and forced convection. Vibrations in the directions of x-axis (#Ch1), y -axis (#Ch2) and z -axis (#Ch3) were obtained by three accelerometers and temperature was obtained by a thermo-couple with respect to time until the motor is steady. Experimental results show that the amplitude of vibration is higher in the order of z-axis (#Ch3), x -axis (#Ch1) and y-axis (#Ch2) and the amplitude of vibration at the forced convection conditions is 10.6% to 17.8% lower than that of vibration at the natural convection. However, the ratio of the vibration value is similar on average regardless of external convection condition.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1757-1763
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• 2011

The physico-chemical properties of broccoli sprouts cultivated in a plant factory system with different lighting conditions were investigated. We reported that there were significant differences among the chemical and nutritional properties of the broccoli sprouts grown under different light sources. Two kinds of sugars (glucose and fructose) were detected in the plants. The amount of glucose was 2.94~3.12% and that of fructose was 1.54~2.04%. Total chlorophyll was $1,157{\pm}0.004$ mg% and chlorophyll-a $777{\pm}0.01$ mg%. All over the test materials, 2 kinds of organic acids (citric acid and malic acid) were detected. The level of citric acid was 908~1,136 mg% and its highest level was under the (Blue LED) light source. The level of malic acid was 514~834 mg% and its highest level was under the (Red LED) light source. Seven different minerals were also analyzed. The amount of K was 518 mg% and its amount was significantly higher than that of Mg or Na. There were also negligible amounts of Zn, Fe and Cu. The amounts of vitamin A, C and E under the Red LED were, $860.62{\pm}0.02\;{\mu}gRE$, $134.570{\pm}0.14$ mg% and $1.44{\pm}0.1$ IU. The amounts under the Blue LED were, $432.48{\pm}0.05\;{\mu}gRE$, $137.05{\pm}0.1$ mg% and $1.11{\pm}0.12$ IU. The amounts under both Red and Blue LEDs were, $667.33{\pm}0.11\;{\mu}gRE$, $118.50{\pm}0.09$ mg% and $1.47{\pm}0.1$ IU. And finally, the amounts under a White LED were, $640.25{\pm}0.08\;{\mu}gRE$, $119.87{\pm}0.07$ mg% and $1.31{\pm}0.15$ IU. In this study, significant changes were shown in the chemical and nutritional properties of the broccoli sprouts. These findings indicate that LED light sources stimulated germination of the plants.

• Radiation Oncology Journal
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• v.15 no.3
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• pp.243-249
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• 1997

Purpose : In radiation therapy, NTCF is very importart indicator of selecting the optimal treatment plan. In our study, we tried to find out usefullness of NTCP in lung cancer by comparng the incidence of radiation pneumonitis with NTCP. Materials and Methods : From August 1993 to December 1994, thirty six patients with locally advanced non=small cell lung cancer were treated by concurrent chemoradiation therapy. Total dose of radiation therapy was 6480cGy (120cGy, bid) and chemotherapeutlc agents were mitomycin C. vinblastion, cisplatin (2 cycles, 4 weeks interval). We evaluated the development of raniation pneumonitis by CT scan, chest x-rar and clinical symptoms. We used grading system of South Western Oncology Group (SWOG) for radiation pneumanitis. Dose Volume Histograms (DVH) were analyzed for ipsilateral and whole lung, Non uniform DVH was translated to uniform DVH by effective volume method. With these data, we calculated NTCP for ipsilateral and whole lung. Finally we compared the clinical results to NTCP. Results : Eight of thrity six patients developed radiation pneumonitis. Of these 8 patients , 6 had grade I severity and 2 had grade II. The average NTCP value cf the patients who showed radiation pneumonitis was significantly higher than that uf the patients without pneumonitis $(66\%\;vs.\;26.4\%)$. But the results of pulmonary function test was not correlated with NTCP. Conclusion : NTCP of lung is very good indicator for selecting rival treatment planning in lung cancer. According to the results of NTCP, it may be possible to adjust target volume and optimize target dose. In the near future, we are going to anaiyze the effect of hyperfractionation and concurrent chemotherapy in addition to NTCP.