• Title/Summary/Keyword: BLAST Analysis

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Gene Expression Profiling in Rice Infected with Rice Blast Fungus using SAGE

  • Kim, Sang-Gon;Kim, Sun-Tae;Kim, Sung-Kun;Kang, Kyu-Young
    • The Plant Pathology Journal
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    • v.24 no.4
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    • pp.384-391
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    • 2008
  • Rice blast disease, caused by the pathogenic fungus Magnaporthe grisea, is a serious issue in rice (Oryza sativa L.) growing regions of the world. Transcript profiling in rice inoculated with the fungus has been investigated using the transcriptomics technology, serial analysis of gene expression (SAGE). Short sequence tags containing sufficient information which are ten base-pairs representing the unique transcripts were identified by SAGE technology. We identified a total of 910 tag sequences via the GenBank database, and the resulting genes were shown to be up-regulated in all functional categories under the fungal biotic stress. Compared to the compatible interaction, the stress and defense genes in the incompatible interaction appear to be more up-regulated. Particularly, thaumatin-like gene (TLP) was investigated in determining the gene and protein expression level utilizing Northern and Western blotting analyses, resulting in an increase in both the gene and the protein expression level which arose earlier in the incompatible interaction than in the compatible interaction.

A Study on Determination for Location of Localizer Antenna under Area Restrictive Conditions at Domestic P-Airport (국내 P공항의 부지 제한조건을 고려한 로컬라이저의 최적위치 선정에 관한 연구)

  • Cho, Hwan-Kee;Kim, Jong-Bum;Song, Byung-Heum
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.23 no.2
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    • pp.7-14
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    • 2015
  • This paper deals with an optimal determination process for the built-in location of localizer under restrictive siting area conditions of a domestic P-airport. Aerodynamic forces and moments acting on the localizer structure can be used a reference to find the safe distance from jet blast and the position at which the reasonable structural loading is applied. Wind tunnel experiment is conducted to measure aerodynamic loadings. The finite element analysis for structural deformation is employed to get the information of structural failure. A new localizer's position is determined by considering aerodynamic loading, structural strength and thermal loading due to jet blast. Deflector effect was also investigated in this study. In conclusion, the location of localizer can be placed at shorter than the current position and greatly decreased if the deflector is applied at the front of localizer.

Sulfate Resistance of Concrete using Ground Granulated Blast-furnace Slag for Recycling

  • Moon, Han-Young;Lee, Seung-Tae;Kim, Hong-Sam
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.508-513
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    • 2001
  • Recently, ground granulated blast-furnace slag (GGBFS) has been increasingly used as additive for concrete. Many researchers reported that concrete using GGBFS had a better resistance under severe environments, such as marine or sulfate-rich soils, than Portland type cement concrete. The aim, therefore, of this study is to evaluate on the effectiveness of concrete using GGBFS when the concrete exposes to sulfate-rich environment. The detailed items for experiments show 2 series consisted of sulfate immersion test with mortar and sulfate diffusion test with concrete. The sulfate immersion test was performed for 400 days and contained reduction of compressive strength, length change and XRD analysis. For sulfate diffusion test, sulfate ions diffusivity was calculated on tile consideration of electrochemical theory by the diffusion cell test. As the results of this study, it was found that the concrete using GGBFS as additive was superior to portland type cement concrete. Consequently, the use of concrete with GGBFS for recycling may expect the durable and economical benefits.

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Prediction of the Blast Wave Propagation Over a Kick Motor Test Facility (Kick Motor 시험장 충격파 전파 예측)

  • Ok, Ho-Nam;Kim, In-Sun
    • 한국전산유체공학회:학술대회논문집
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    • 2008.03b
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    • pp.220-223
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    • 2008
  • A test facility to measure the performance of a KM(Kick Motor) is constructed, and prediction of blast wave propagation over the facility is performed to check if the safety of test personnel in MCC(Main Control Center) can be guaranteed even for the most severe explosion. Assuming that the initial explosion energy is contained in a sphere under the pressure of 500, 1000, 1500 psi, respectively, the radius of the sphere is determined for each pressure to set the mass of contained explosion gas to 35 kg. The material properties of explosion gas are set to be the ones of KM propellant combustion gas under normal condition. To reduce the effort and time required for a complex three-dimensional modeling, the flowfield is approximated to axismmetry. Calculations are performed for all three initial pressure conditions, and the analysis of the result is given for 1500 psi which is expected to be the worst case. The maximum pressure is 3.5 psig while the minimum pressure is -1.2 psig on the outer wall of MCC, and the maximum pressure difference between the inner and outer walls of protection wall amounts to 3.0 psi.

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Analysis of Possibilities of Rapid Appraisal of Blast Furnace Slag Cement Fineness Quality Using the Hydrometer Method (Hydrometer법을 이용한 고로슬래그 시멘트의 분말도 신속 품질 평가 가능성 분석)

  • Lee, Jae-Jin;Kim, Min-Sang;Moon, Byeong-Yong;Han, In-Deok;Han, Min-Cheol;Han, Cheon-Goo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.05a
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    • pp.169-170
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    • 2017
  • Recently in the construction industry there has been an increasing use of increasing replacement of cement with blast furnace slag cement(BS), an industrial by-product, to reduce environment load and production costs, and other increasing uses of BS cement. However in the case of BS being delivered in RMC factories, only score reports submitted when the are delivered are relied upon and ways to test the quality of fineness is inadequate. Therefore this study gave many changes to various cements and BS fineness to test the variations of BS as a cement, then used the correlation between this and the density value in the Hydrometer method to test the possibility for rapid appraisal of quality fineness of BS cement. Results showed that if the one fifth graph of the density and fineness correlation were to be used, a rapid appraisal of BS cement fineness quality would be possible.

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Analysis on Basic Characteristics of Mortar with Pre-Mix Cement (프리믹스 시멘트를 사용한 모르터의 기초적 특성 분석)

  • Jin, Cheng-Ri;Jang, Ki-Hyun;Lee, Chung-Sub;Han, Dong-Yeop;Kim, Ki-Hoon;Han, Cheon-Goo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2008.05a
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    • pp.93-97
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    • 2008
  • The study analyzed on each kind of basic characteristics in mortar to prove an effect of pre-mix cement, ultra high strength binder. The results were as follows. in characteristics of not set mortar, fluidity time was the quickest at the time of using POBSA and was more delayed at the time of using Fly Ash than at the time of using Blast Furnace Slag as a binder. Mortar ring flow and flow con flow got smaller as fluidity time got longer, on the contrary to fluidity time. Unit capacity mass was smaller at the time of using Fly Ash than at the time of using Blast Furnace Slag. According to pre-mix, it was difficult to discover a regular tendency. In characteristics of hardening mortar, the 28th day compressive strength was better than thing mixed after measuring separately in pre-mix. According to a binder's kind, Silica Fume B, C was better than Silica Fume A.

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Performance Analysis and Design of MIMO Systems for Terrestrial Transmission of UHDTV (UHDTV를 위한 MIMO 전송 시스템 성능 분석 및 설계)

  • Jo, Bong-Gyun;Han, Dong-Seog
    • Journal of Broadcast Engineering
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    • v.15 no.4
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    • pp.547-554
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    • 2010
  • In this paper, we propose transmission systems for ultra high definition television (UHDTV) through terrestrial transmission by applying the multi-input multi-output (MIMO) technology. The space time block code, hybrid STBC, V-BLAST and linear dis- persion code are considered to support a high data rate of the UHDTV system. The performance of proposed MIMO systems are evaluated through computer simulations. Then we suggest MIMO parameters, number of antennas and optimal transmission scheme to achieve the transmission rate of the UHDTV system.

The effect of in-plane deformations on the nonlinear dynamic response of laminated plates

  • Kazanci, Zafer;Turkmen, Halit S.
    • Structural Engineering and Mechanics
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    • v.42 no.4
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    • pp.589-608
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    • 2012
  • In this study, the effect of in-plane deformations on the dynamic behavior of laminated plates is investigated. For this purpose, the displacement-time and strain-time histories obtained from the large deflection analysis of laminated plates are compared for the cases with and without including in-plane deformations. For the first one, in-plane stiffness and inertia effects are considered when formulating the dynamic response of the laminated composite plate subjected to the blast loading. Then, the problem is solved without considering the in-plane deformations. The geometric nonlinearity effects are taken into account by using the von Karman large deflection theory of thin plates and transverse shear stresses are ignored for both cases. The equations of motion for the plate are derived by the use of the virtual work principle. Approximate solution functions are assumed for the space domain and substituted into the equations of motion. Then, the Galerkin method is used to obtain the nonlinear algebraic differential equations in the time domain. The effects of the magnitude of the blast load, the thickness of the plate and boundary conditions on the in-plane deformations are investigated.

Technical and economical feasibility of using GGBS in long-span concrete structures

  • Tang, Kangkang;Millard, Steve;Beattie, Greg
    • Advances in concrete construction
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    • v.3 no.1
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    • pp.1-14
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    • 2015
  • China accounts for nearly half of the global steel production. As a waste material or a by-product in the manufacture process, a large amount of blast furnace slag is generated every year. The majority of recycled blast furnace slag is used as an additive in low-grade blended cement in China (equivalent to the UK CEM II or CEM III depending on the slag content). The cost of using ground granulated blast furnace slag (GGBS) in such low-grade applications may not be entirely reimbursed based on market research. This paper reports an on-going project at Xi'an Jiaotong-Liverpool University (XJTLU) which investigates the feasibility of using GGBS in long-span concrete structures by avoiding/reducing the use of crack control reinforcement. Based on a case study investigation, with up to 50% of CEM I cement replaced with GGBS, a beneficiary effect of reduced thermal contraction is achieved in long-span concrete slabs with no significant detrimental effect on early-age strengths. It is believed that this finding may be transferable from China to other Asian countries with similar climates and economic/environmental concerns.

Genomic Insights into the Rice Blast Fungus through Estimation of Gene Emergence Time in Phylogenetic Context

  • Choi, Jaeyoung;Lee, Jong-Joon;Jeon, Junhyun
    • Mycobiology
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    • v.46 no.4
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    • pp.361-369
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    • 2018
  • The rice blast fungus, Magnaporthe oryzae, is an important pathogen of rice plants. It is well known that genes encoded in the genome have different evolutionary histories that are related to their functions. Phylostratigraphy is a method that correlates the evolutionary origin of genes with evolutionary transitions. Here we applied phylostratigraphy to partition total gene content of M. oryzae into distinct classes (phylostrata), which we designated PS1 to PS7, based on estimation of their emergence time. Genes in individual phylostrata did not show significant biases in their global distribution among seven chromosomes, but at the local level, clustering of genes belonging to the same phylostratum was observed. Our phylostrata-wide analysis of genes revealed that genes in the same phylostratum tend to be similar in many physical and functional characteristics such as gene length and structure, GC contents, codon adaptation index, and level of transcription, which correlates with biological functions in evolutionary context. We also found that a significant proportion of genes in the genome are orphans, for which no orthologs can be detected in the database. Among them, we narrowed down to seven orphan genes having transcriptional and translational evidences, and showed that one of them is implicated in asexual reproduction and virulence, suggesting ongoing evolution in this fungus through lineage-specific genes. Our results provide genomic basis for linking functions of pathogenicity factors and gene emergence time.