• Korean Journal of Exercise Nutrition
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• v.23 no.4
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• pp.6-13
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• 2019

[Purpose] In this study, we examined the in vitro antioxidant activities and laxative effects of taurine-xylose (T-X), a synthetic taurine-carbohydrate derivative, in a rat model of constipation induced by loperamide. [Methods] The animals were divided into four treatment groups: normal untreated rats (NOR group), loperamide-treated control rats (CON group), loperamide and taurine-xylose (15 mg/kg)-treated rats (T-X group), and loperamide and commercial Dulcolax S (5.5 mg/kg)-treated rats (DS group). [Results] In the present study, T-X exhibited potent reducing power and free radical scavenging activities for DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS+ (2,2'-azino-bis (3-ethylbenzothiazoline 6-sulfonic acid ammonium salt) radicals. The laxative effects of T-X were dependent on food, body weight, fecal properties, gastrointestinal transit (GIT) ratio, and serum metabolic parameters. In the T-X group, the number, wet weight, and water content of fecal pellets were noticeably increased compared to those in the loperamide-induced group. T-X treatment significantly increased the activities of hepatic antioxidant enzymes, including those of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), relative to those in loperamide-induced constipated rats. Furthermore, the GIT ratio and loperamide-induced metabolic parameters in serum, including gastrin (GAS), motilin (MTL), and somatostatin (SS) levels, were significantly improved by T-X treatment. [Conclusion] These results suggest that taurine-xylose exerts antioxidant activities and laxative effects on loperamide-induced constipation by promoting gastrointestinal motility.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.82-82
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• 2003

Offspring have been produced from somatic cells in a number of species. This biotechnology introduced a new phenomenon in reprogramming and differentiation of somatic cell, namely totipotency. However, birth of oversized calves and perinatal abnormalities such as increased gestation length, lack of spontaneous parturition, higher incidence of dystocia, and reduced perinatal viability of offspring are frequently observed in pregnancies of cloned bovine fetuses. Disturbance of feto-placenta has been proposed as likely causes for abnomal growth. However. Little is known the mechanism responsible for the perinatal problems. Therefore, we focused on gestation length in somatic cell nuclear recipient cows. To solve this issues, placental tissues of control and cloned bovine were obtained by a cesarean section (C-section) before 5 days of paturition. Total RNA from control and cloned bovine placenta was extractd by TRIzol reagent. GeneFishing DEG kits (Seegene) were used to identify differentially expression genes. Total RNA (3 ug) were synthesized by M-MLV reverse transcriptase (200 u/ul) with 10 uM dT-annealing control primer (ACP1) at 42C for 90 min. Then, first-strand cDNA (50 ng) was amplified using the 5 uM arbitary ACP (1-20) and 10 uM dT-ACP2 primers. Some specific expression genes were amplified, Now, we are cloning and sequencing. These finding strongly can be support to solve the problems for parturition delay in nuclear transfer cows, suggest that placenta specific proteins are key indicators for the aberration of gestation and placental function in cows.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.176-182
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• 2017

This study was conducted to evaluate the protein requirement for maintenance of fattening Korean black goat (Capra hircus coreanae). Six male goats with average initial body weight (BW) of $31.78{\pm}4.54kg$ and an average age of 8 months were used in this study. The experiment had a replicated duplicated $3{\times}3$ Latin square design for balancing carryover effects. In the course of the experiment, each of Black goats were fed three diets that were formulated to contain T1 (13%), T2 (16%) and T3 (19%) levels of crude protein (CP). A 14-day diet adjustment period was followed by a 5-day collection period. Dry matter intake (DMI) of groups fed diets with T2 was 966.67g/d which was higher than group fed diets with T1 and T3 were 925.14g/d and 936.08g/d each. Average daily gains (ADG) of black goats were the highest in T2(167.13g/d) But, there was no significant difference. Dietary protein levels affected the apparent digestibility of CP (p<0.05). A significant difference was found in CP intake among treatments and goats receiving T3, T2, and T1 recorded 181.23, 154.57, and 128.78g CP/d, respectively. This was excepted because CP intake is proportional to CP content of diet, which from highest to lowest was as follows: T3 (19%) > T2 (16%) > T1 (13%). Intercept of the regression equation between CP intake and CP balance indicated that maintenance CP requirement was 1.63g/BW0.75.

• Journal of KIISE:Computer Systems and Theory
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• v.34 no.9
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• pp.416-424
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• 2007

The Bio-Inspired system is a computing model that emulates the objects in ecosystem which are evolving themselves and cooperate each other to perform some tasks. Since it could be used to solved the complex problems that have been very difficult to resolve with previous algorithms, there have been a lot of researches to develop an application based on the Bio-Inspired system. However, since this computing model requires the process of evolving and cooperating with a lot of objects and this process takes a lot of times, it has been very hard to develop an application based on this computing model. This paper presents a parallel simulator for a Bio-Inspired system that is designed and implemented with OMNeT++ on PC clusters, and proves its usefulness by showing its simulation performance for a couple of applications. In the proposed parallel simulator, the functions required in the ERS platform for evolving and cooperating between objects (called Ecogent) are mapped onto the functions of OMNeT++, and they are simulated on PC clusters simultaneously to reduce the total simulation time. The simulation results could be monitored with a GUI In realtime, and they are also recorded into DBMS for systematic analyses afterward. This paper shows the usefulness of the proposed system by analyzing its performances for simulating various applications based on Bio-Inspired system on PC clusters with 4 PCs.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.11-17
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• 2007

Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

• Journal of Environmental Health Sciences
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• v.47 no.5
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• pp.410-424
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• 2021

Background: Subway stations have the characteristics of being located underground and are a representative public-use facility used by an unspecified number of people. As concerns about indoor air quality (IAQ) increase, various management measures are being implemented. However, there are few systematic studies and cases of long-term continuous measurement of underground station air quality. Objectives: The purpose of this study is to analyze changes and factors influencing IAQ in subway stations through real-time continuous long-term measurement using IoT-based IAQ sensing equipment, and to evaluate the IAQ improvement effect of a bio-filter system. Methods: The IAQ of a subway station in Seoul was measured using IoT-based sensing equipment. A bio-filter system was installed after collecting the background concentrations for about five months. Based on the data collected over about 21 months, changes in indoor air quality and influencing factors were analyzed and the reduction effect of the bio-filter system was evaluated. Results: As a result of the analysis, PM₁₀, PM_2.5, and CO₂ increased during rush hour according to the change in the number of passengers, and PM₁₀ and PM_2.5 concentrations were high when a PM warning/watch was issued. There was an effect of improving IAQ with the installation of the bio-filter system. The reduction rate of a new-bio-filter system with improved efficiency was higher than that of the existing bio-filter system. Factors affecting PM_2.5 in the subway station were the outdoor PM_2.5, platform PM_2.5, and the number of passengers. Conclusions: The IAQ in a subway station is affected by passengers, ventilation through the air supply and exhaust, and the spread of particulate matter generated by train operation. Based on these results, it is expected that IAQ can be efficiently improved if a bio-filter system with improved efficiency is developed in consideration of the factors affecting IAQ and proper placement.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.26-33
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• 1990

The pot experiment was conducted to compare the effects of BIO-COM (Organic fertilizer made from industrial by-product) with the compost and humic acid on the physico-chemical properties of soils and the yield of paddy rice (CV. Chucheong-byeo). The results obtained were as follows: 1. All the fertilizer treatments, except humic acid, increased rice yield compare to no fertilizer treatment, and Bio-Com was the highest among the fertilizer treatments. 2. Rice yields were increased with increasing BIO-COM only up to 4% but 2% with N.P.K. fertilizers. 3. Rice yield showed highly positive correlation with number of panicle per hill but negative correlation with 1000 grain weight. 4. BIO-COM application induced the noticeable increase of pH, EC, OM, T-N, Av-P, Ex-Ca, Ex-Mg, Ex-Na, and $Av-SiO_2$, contents in the soils after experiments, and there were highly significant positive correlation among them. 5. BIO-COM application was positively affected on plant height, number of tiller, and fresh weight and dry weight of rice plant throughout entire growing stages and these are positively correlated with rice yield. 6. BIO-COM application also induced the increase of chlorophyll, N, P, Ca, and Mg contents in rice plant 7. It is revealed that there were highly positive correlations between rice yields and pH, EC, OM, T-N, Ex-Ca, and Ex-Mg in the soils. 8. It was also shown the highly significant positive correlations between rice yields, and N and P contents in the rice plant at heading stages, respectively.

• Development and Reproduction
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• v.14 no.2
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• pp.59-63
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• 2010

This study was performed to examine the influence of water temperature on egg developmental speed for determining the required time and optimum water temperature for hatching of olive flounder Paralichthys olivaceus eggs. The fertilized eggs were collected from the naturally spawned adults in November 2007. The eggs were randomly divided into 6 groups of temperature (5, 10, 15, 20, 25 and $30^{\circ}C$) and transferred in $1{\ell}$ beaker, respectively. The fertilized eggs of the olive flounder did not hatched at $5^{\circ}C$ and $30^{\circ}C$ and hatching rates at 10, 15, 20 and $25^{\circ}C$ were 3, 12, 25 and 50%, respectively. The relationships between the water temperature (T, $^{\circ}C$) and required time (1/t, hour) from egg to each developmental stage were given as follows ; Blastula: 1/t=0.0208T-0.0951 ($r^2$=0.8593) Kupffer's vesicle: 1/t=0.0052T-0.0176 ($r^2$=0.9819) Myotome: 1/t=0.0034T-0.0172 ($r^2$=0.8508) Hatching: 1/t=0.0016T-0.0068 ($r^2$=0.9915) Biological minimum temperature in egg development was calculated to be $4.3^{\circ}C$.

• Journal of The Korean Society of Agricultural Engineers
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• v.58 no.6
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• pp.47-54
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• 2016

This study is a basic research on the functional performance review for the use of environmentally friendly construction materials as lapilli-bio block. The neutralization processed Mt. Backdusan and Mt. Hallasan lapilli-bio block were identified vegetation characteristics and water purification properties. As a result, the adsorption of root was more favorable, depending on the material properties of many voids Lapilli. And if the neutralization processed lapilli-bio blocks maintain a long-term immersion conditions in contaminated water was found to be expected from the improvement effect of SS, T-N and T-P. However, field trials and continuous studies are expected to be identified by the use of lapilli-bio block.

• Journal of environmental and Sanitary engineering
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• v.18 no.1
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• pp.23-29
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• 2003

The effect of ozone oxidation on bio-degradability of leachate was studied. Ozone oxidation process was used as pre-treatment process to enhance performance of biological process in treating landfill leachate. Optimum ozone injection rate and contact time in this experiment was $160{\;}mg{\;}O_/{\ell}{\cdot}hr$ and 45 minutes, respectively. Bio-degradability was enhanced 5.08% by ozone oxidation. The ratio of ozone demand/DOC concentration was $0.049~0.091{\;}mg{\;}O_3/mg{\;}DOC$. The increase of bio-degradability depending on ozone injection rate(D) and contact time(T) can be expressed as follows ; The rate of bio-degradation of DOC was increased proportionally with the increase of ozone injection rate and contact time irrespective of landfill site age. The increase of bio-degradability by ozone addition was not satisfactory. It is hard to expect significant increase in bio-degradability by ozone treatment only. Thus, it is evaluated that ozone oxidation can not increase biodegradability significantly in concentrated wastewater composed of complex organic compound such as leachate.