• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.73.1-73
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• 2003

In order to cultivate watermelon on farm, grafting of the watermelon seedling to the watermelon stock is necessary because the watermelon root is less viable than the root of watermelon stock. Recently, commercially important watermelon varieties further require a resistant stock against especially CGMMV to control the heavy loss of the total yield of watermelon by CGMMV infection. Therefore, we have set out a project to develop a CGNEMV-resistant watermelon stock. We have successfully transformed dozens of watermelon stocks (gongdae) during last two years especially using a cDNA encoding the coat protein of CGMMV (cucumber green mottle mosaic virus). Recently we have tested levels of resistance of those watermelon stocks against CGMMV infection. For CGMMV inoculation, the leaves of one month old gongdae (T1) were rubbed by carborundum mixed with the CGMMV. A total of 140 plants (T1) were exposed to the CGMMV and we found that ten plants were completely resistant to virus infection. This is the first report that by genetic engineering a cucubitaceae crop resistant to CGMMV infection is ever developed. Further information will be provided in the poster.

• Genomics & Informatics
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• 제13권3호
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• pp.81-85
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• 2015

Molecular characterization technology in genetically modified organisms, in addition to how transgenic biotechnologies are developed now require full transparency to assess the risk to living modified and non-modified organisms. Next generation sequencing (NGS) methodology is suggested as an effective means in genome characterization and detection of transgenic insertion locations. In the present study, we applied NGS to insert transgenic loci, specifically the epidermal growth factor (EGF) in genetically modified rice cells. A total of 29.3 Gb (${\sim}72{\times}coverage$) was sequenced with a $2{\times}150bp$ paired end method by Illumina HiSeq2500, which was consecutively mapped to the rice genome and T-vector sequence. The compatible pairs of reads were successfully mapped to 10 loci on the rice chromosome and vector sequences were validated to the insertion location by polymerase chain reaction (PCR) amplification. The EGF transgenic site was confirmed only on chromosome 4 by PCR. Results of this study demonstrated the success of NGS data to characterize the rice genome. Bioinformatics analyses must be developed in association with NGS data to identify highly accurate transgenic sites.

• 한국토양비료학회지
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• 제45권4호
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• pp.657-663
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• 2012

호소저수구역에서 수온이 침수식물체의 영양염류 용출에 미치는 영향을 평가하기 위해 $5^{\circ}C$ 및 $25^{\circ}C$ 조건의 칼럼에서 이삭사초와 물억새를 침수시킨 후 COD, T-N 및 T-P 함량을 조사하였다. 이삭사초의 침수 후 60일째 COD 용출량은 $5^{\circ}C$ 조건에서 $60.4mg\;L^{-1}$이었으며, $25^{\circ}C$ 조건에서 $78.0mg\;L^{-1}$이었다. 물억새의 침수 후 60일째 COD 용출량은 $5^{\circ}C$ 조건에서 $62.5mg\;L^{-1}$이었으며, $25^{\circ}C$ 조건에서 $70.5mg\;L^{-1}$이었다. 전반적으로 이삭사초에서는 $25^{\circ}C$ 조건이 $5^{\circ}C$ 조건에서 보다 COD 용출이 급증한 시기가 초기에 나타난 반면에 물억새에서는 COD 용출이 급증하는 시기가 온도별로 서로 유사한 경향으로 나타났다. 이삭사초의 침수 후 60일째 T-N 용출량은 $5^{\circ}C$ 조건에서 $45.8mg\;L^{-1}$이었으며, $25^{\circ}C$ 조건에서 $60.0mg\;L^{-1}$이었다. 물억새의 침수 후 60일째 T-N 용출량은 $5^{\circ}C$ 조건에서 $55.7mg\;L^{-1}$이었으며, $25^{\circ}C$ 조건에서 $61.0mg\;L^{-1}$이었다. 식생 종류에 관계없이 $25^{\circ}C$ 칼럼의 T-N 용출이 급격히 증가한 시기는 $5^{\circ}C$ 칼럼에서 보다 더 빠른 시기에 나타났다. 이삭사초의 침수 후 60일째 T-P 용출량은 $5^{\circ}C$ 조건에서 $5.7mg\;L^{-1}$이었으며, $25^{\circ}C$ 조건에서 $8.7mg\;L^{-1}$이었다. 물억새의 침수 후 60일째 T-P 용출량은 $5^{\circ}C$ 조건에서 $7.1mg\;L^{-1}$이었으며 $25^{\circ}C$ 조건에서 $8.2mg\;L^{-1}$이었다. 식생 종류에 관계없이 $25^{\circ}C$ 칼럼의 T-P 용출이 급격히 증가한 시기는 $5^{\circ}C$ 칼럼에서 보다 더 빠른 시기에 나타났다. $25^{\circ}C$로 유지된 칼럼의 COD, T-N 및 T-P 용출량은 전반적으로 $5^{\circ}C$로 유지된 칼럼에 비해 높았다.

• 한국식품영양과학회지
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• 제39권8호
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• pp.1102-1106
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• 2010

본 연구는 진피 에탄올 추출물의 alloxan에 의해 유도된 HIT-T15 세포의 산화적 손상으로부터 세포 생존율 및 인슐린분비 조절능에 대해 조사하였다. 진피 에탄올 추출물의 총 폴리페놀 함량과 총 플라보노이드 함량을 측정한 결과, 각각 $57.00{\pm}2.91\;mg/g$, $8.11{\pm}2.83\;mg/g$으로 나타났으며 alloxan 처리 농도가 증가됨에 따라 HIT-T15 세포의 생존율은 감소하였으며, alloxan 11.58 mM에서 약 50%의 세포 독성이 일어남을 확인하였다. 동결건조 된 진피 에탄올 추출물(CP-Et) 0.125~0.75 mg/mL 농도에서 무처리구의 세포 생존율은 $100.90{\pm}1.51{\sim}97.56{\pm}0.73%$로 나타나 HIT-T15 세포에 대한 CP-Et의 처리 농도는 0.125~0.75 mg/mL로 결정하였다. 0.125, 0.25, 0.5 mg/mL 농도의 CP-Et를 처리한 결과, alloxan에 의해 유도된 산화적 세포손상으로부터 세포 생존율이 각각 $80.52{\pm}3.29$, $74.17{\pm}6.75$, $67.53{\pm}5.8%$로 나타나 유의성 있게 보호되어짐을 확인하였다. CP-Et를 처리한 HIT-T15 세포에서는 산화적 손상에 대한 세포 생존율과는 다르게 0.125 mg/mL 농도에서 $116.93{\pm}2.11{\mu}g/mg$로 인슐린 분비가 대조구와 비교 시 유의적으로 증가되었음을 확인 할 수 있었다. 따라서 진피 에탄올 추출물에 의한 항산화적 방어로 2형 당뇨의 $\beta$-cell 양의 감소와 인슐린 저항성에 대한 개선이 가능함을 제시하며, 그 작용 기작에 대해서는 차후 더 많은 연구가 필요하다 하겠다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1087-1095
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• 2020

Objective: The present study aimed to evaluate the effects of γ-aminobutyric acid (GABA)-producing bacteria (GPB) on in vitro rumen fermentation and on the growth performance and meat quality of Hanwoo steers. Methods: The effects of GPB (Lactobacillus brevis YM 3-30)-produced and commercially available GABA were investigated using in vitro rumen fermentation. Using soybean meal as a substrate, either GPB-produced or commercially available GABA were added to the in vitro rumen fermentation bottles, as follows: control, no additive; T1, 2 g/L GPB; T2, 5 g/L GPB; T3, 2 g/L autoclaved GPB; T4, 5 g/L autoclaved GPB; T5, 2 g/L GABA; and T6, 5 g/L GABA. In addition, 27 Hanwoo steers (602.06±10.13 kg) were subjected to a 129-day feeding trial, during which they were fed daily with a commercially available total mixed ration that was supplemented with different amounts of GPB-produced GABA (control, no additive; T1, 2 g/L GPB; T2, 5 g/L GPB). The degree of marbling was assessed using the nine-point beef marbling standard while endotoxin was analyzed using a Chromo-Limulus amebocyte lysate test. Results: In regard to in vitro rumen fermentation, the addition of GPB-produced GABA failed to significantly affect pH or total gas production but did increase the ammonia nitrogen (NH₃-N) concentration (p<0.05) and reduce total biogenic amines (p<0.05). Animals fed the GPB-produced GABA diet exhibited significantly lower levels of blood endotoxins than control animals and yielded comparable average daily gain, feed conversion ratio, and beef marbling scores. Conclusion: The addition of GPB improved in vitro fermentation by reducing biogenic amine production and by increasing both antioxidant activity and NH₃-N production. Moreover, it also reduced the blood endotoxin levels of Hanwoo steers.

• Investigative Magnetic Resonance Imaging
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• 제14권1호
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• pp.10-20
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• 2010

목적: 최근, 미국 애리조나 세도나에서 열린 국제자기공명학회 (ISMRM) 주관의 2009년 데이터 샘플링과 영상 복원에 관한 워크샵에서 자기공명영상 복원 대회가 열렸다. 이 대회는 time resolved contrast enhanced MR angiography 에 대한 고속 촬영의 실제 활용 가능성을 평가하기 위한 것이었다. 본 논문은 이 대회의 우승 결과를 얻은 k-t FOCUSS 알고리듬을 단계별로 자세히 묘사하도록 한다. 대상 및 방법: 본 그룹은 앞선 연구에서 비교적 덜 스파스한 심장 영상에 대해 k-t FOCUSS 알고리듬이 성공적으로 압축센싱 문제를 풀수 있음을 증명했다. 따라서 k-t FOCUSS 알고리듬을 time resolved contrast enhanced MR angiography 에 적용함으로써, 매우 정확한 영상 복원이 가능할 것이다. 영상 복원을 위해 X-ray 대뇌 혈관조영 영상으로부터 구성된 다운 샘플링된 데이터가 대회 주최측으로부터 공통으로 제시되었고, 방사선과 의사들이 각 복원된 영상에 대한 사전 정보 없이, 원래 영상과 복원된 결과를 비교함으로써, 영상의 질을 평가하였다. 결과: 다양한 다운샘플링에 대해 얻어진 결과들은 영상의 스파스 변환이나 샘플링 형태와 같은 압축센싱의 중요한 요소들에 의해 크게 영향을 받는다는 것을 보여주었다. 결론: 복원된 결과로부터, 압축센싱 동적자기공명영상 기법인 k-t FOCUSS 가 고해상도의 time resolved contrast enhanced MR angiography 를 가능하게 할 수 있음을 확인하였다.

• 한국토양비료학회지
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• 제45권4호
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• pp.642-648
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• 2012

주암호 상수원 상류지역인 죽산천에 위치한 죽산천 인공 습지의 수생태학적 특성을 평가하기 위해 수질에서의 영양 염류 처리효율, 수생식물의 무기성분 흡수량 및 퇴적물내 화학적 특성을 평가하였다. 수질에서의 영양염류 처리효율은 COD와 SS를 제외하고 전반적으로 낮았다. 죽산천 인공 습지의 주요 우점종의 영양염류 흡수량을 조사한 결과, T-N 및 T-P 흡수량은 8월에 노랑꽃창포 > 수련 > 노랑어리 연꽃 순으로 최대흡수량을 나타내었다. 퇴적물내 O.M 함량은 시기별로 큰 차이가 없었고, T-N 및 T-P의 함량은 봄에서 여름으로 시기가 변함에 따라 낮아져서 겨울까지 낮은 함량을 유지하였다. 퇴적물내 microbial biomass C:N:P의 비율은 봄, 여름, 가을 및 겨울이 각각 117~140:1~4:1, 86~126:5~6:1, 68~101:2~6:1 및 47~138:2~4:1로 나타났다. 죽산천 인공습지는 질소와 인의 처리효율이 낮고, 겨울철 수질 정화효율이 낮아 습지의 정화효율 향상을 위한 개선이 필요할 것으로 판단된다.

• 대한한의학방제학회지
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• 제25권4호
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• pp.457-469
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• 2017

Obesity is one of the most serious health problem because it induced numerous metabolic syndrome and increases the incidence of various disease, including diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer. In 3T3-L1 adipocytes, increases in reactive oxygens species (ROS) occur with lipid accumulation. NADPH oxidase, producing superoxide anion, may contribute to the development of obesity-associated insulin resistance and type 2 diabetes. In this study, we elucidated the effect of Chaenomeles sinensis koehne extract (CSE) against the development of obesity and the inhibition mechanisms in 3T3-L1 preadiocytes. CSE decreased triglyceride content and inhibited the expression of adipogenic transcription factors including peroxisome proliferator-activated receptor $(PPAR){\gamma}$, CCAT/enhancer binding protein $(C/EBP){\alpha}$ and sterol regulatory element-binding protein (SREBP-1). In addition, CSE highly increased antioxidant activity in a dose-dependent manner. CSE remarkably reduced intracellular ROS increase and NAD(P)H oxidase activity, NOX1, NOX4, Rac1 protein expression, and phosphorylation of p47phox and p67phox We also studied the effect of CSE on weight gain induced by high-fat diet. The oral treatment of CSE (500 mg/kg, body weight) in diet-induced obese (DIO) mice showed decrease in triglyceride and adipocyte size. Therefore, these results indicate that the effect of CSE on anti-obese effects, adipocyte differentiation and reducing triglyceride contents as well as adipocyte size in obese mice, may be associated with inhibition of NAD(P)H oxidase-induced ROS production and adipose transcription factors. These results showed the potential to inhibit the obesity by CSE treatment through controlling the activation of NAD(P)H oxidase in vitro and in vivo obese model.

• Journal of Pharmaceutical Investigation
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• 제34권5호
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• pp.413-418
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• 2004

A bioequivalence of $Melax^{TM}$ capsules (Chong Kun Dang Pharm., Korea) and $Mobic^{TM}$ capsules (Boehringer Ingelheim Korea) was evaluated according to the guideline of Korea Food and Drug Administration (KFDA). Single 15 mg dose of meloxicam of each medicine was administered orally to 24 healthy male volunteers. This study was performed in a $2\;{\times}\;2$ crossover design. Concentrations of meloxicam in human plasma were monitored by a high-performance liquid chromatography. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 72 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance was performed using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for $Melax^{TM}/Mobic^{TM}$ were 0.95 - 1.04 and 0.98 - 1.14, respectively. This study demonstrated a bioequivalence of $Melax^{TM}$ and $Mobic^{TM}$ with respect to the rate and extent of absorption.

• Journal of Pharmaceutical Investigation
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• 제37권4호
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• pp.255-261
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• 2007

A bioequivalence study of $Nimegen^{TM}$ soft capsule (Medica Korea Pharma. Co., Ltd.) to $RoAccutane^{(R)}$ soft capsule (Roche Korea Ind. Co., Ltd.) was conducted according to the guidelines of Korea Food and Drug Administration (KFDA). Thirty healthy male Korean volunteers received each medicine at the isotretinoin dose of 60 mg in a $2{\times}2$ crossover study. There was one week wash-out period between the doses. Plasma concentrations of isotretinoin were monitored by a high performance liquid chromatography (HPLC) for over a period of 48 hours after drug administration. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 48 hr) was calculated by the linear trapezoidal rule method. $C_{MAX}$ (maximum plasma drug concentration) and $T_{MAX}$ (time to reach $C_{MAX}$) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t\;and\;C_{MAX}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{MAX}$ ratio for $Nimegen^{TM}/RoAccutane^{(R)}$ were $log0.860{\sim}log0.98\;and\;log0.85{\sim}log1.00$, respectively. These values were within the acceptable bioequivalence intervals of $log0.80{\sim}log1.25$. Thus, our study demonstrated the bioequivalence of $Nimegen^{TM}\;and\;RoAccutane^{(R)}$ with respect to the rate and extent of absorption.