• Journal of Microbiology
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• v.33 no.3
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• pp.252-259
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• 1995

The PCR was employed to detect and characterize the bovine immunodeficiency-like virus (BIV), which is a newly recognized member of the I entivirinae of the retroviruses. Degenerate primers representing the conserved regions in the pol genes of the Lentivirinae, were used to detect proviral DNA obtained from the bovine embryonic spleen cell cultures infected with BIV. The PCR amplified DNA fragment was molecularly cloned and sequenced. The BIV DNA fragment contained a sequence identical to that reported by Garvey et al. (Garvey et al., 1990. Virology, 175, 391-409). With the degenerate primers, peripheral blood mononuclear cells (PBMCs) of sick cattle and cells cultured with BIV were tested to determine genetic variation of BIV pol conserved sequence. We found the sequence heterogeneity within cultures and most variations occurred at the third base of codons that would not lead to amino acid substitutions. Another change was GAG (Glu) to AAG (Lys) within the BIV isolates. Interestingly, the altered sequence is also found in other lentiviruses such as HIV-2, SIV mac, CAEV and EIAV.

• Biomedical Science Letters
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• v.5 no.1
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• pp.101-107
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• 1999

Bovine immunodeficiency virus (BIV) which was grouped into the Lentivirinae of family Retroviridae, was known to be causing many immunodeficiency syndromes among cows. The BIV was studied worldwide during last several years for its importance in cattle industries but nothing was reported in Korea until now Thus we initially tried to study the existence of BIV in cattle around the Daegu·Kyungpook area by PCR related molecular techniques. As a prerequisite investigation for detecting Korean-type BIV, we had focused our aim into BLV infected cows because the BLV infected cows tend to show BIV infection with 5％ ranges. Hence we randomly sampled fresh bloods from 248 cows and bulls near the Daegu·Kyungpook area and collected peripheral blood monocytes (PBMC) from the sample bloods. After extracting genomic DNA from the PBMC, we subjected it to PCR and Soluthern blot analysis for BIV/BLV detection. Overall, 66.9% (81/121) of the cow PBMC samples turned out to be BLV positive by PCR and the result was reconfirmed by Southern blot analysis. The value was two times higher than the previously reported results of BLV infection in Korea. The significant difference was mainly due to 1) applying highly specific methods for BLV detection such as PCR 2) that BLV was continuously spreaded in the Daegu Kyungpook area without any notice during last ten years. We also tested the BLV positive samples with the same techniques for BIV detection. And we found some BIV positives among the lot 3C samples by PCR, which had showed 100% BLV positive.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.154-154
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• 2021

Since the development of Bubble Image Velocimetry (BIV) technique as the complementary technique of Particle Image Velocimetry (PIV), the application of digital imaging technique in the field of hydraulic and coastal engineering increased rapidly. BIV works very well in multi-phase flow (air-water) flows where the PIV technique doesn't. However, the velocity field obtained from BIV technique often resulted in a velocity vector on the outside of the flow (false velocity) since the Field of View (FOV) usually not only cover the air-water flow but also the area outside the flow. In this study, a simple technique of post processing velocity field was developed. This technique works based on the average of the pixel value in the interrogation area. An image of multi-phase flow of wave overtopping was obtained through physical experiment using BIV technique. The velocity calculation was performed based on the similar method in PIV. A velocity masking technique developed in this study then applied to remove the false velocity vector. Result from non-masking, manually removed and auto removed false velocity vector were presented. The masking technique show a similar result as manually removed velocity vector. This method could apply in a large number of velocity field which is could increase the velocity map post-processing time.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.615-623
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• 1995

Sera from 304 Holsteins or Korean native cattle were collected from slaughterhouse in Kwangju area to study the infection of major virus-borne diseases. Serum antibody titers against infectious bovine rhinotracheitis virus(IBRV), bovine viral diarrhea virus(BVDV), parainfluenza type-3 virus(PI-3V), bovine ephemeral fever virus(BEFV), bovine Ibaraki virus(BIV), bovine Akabane virus(BAKV), bovine rotavirus(BRV), bovine coronavirus(BCV) were measured by serum neutralization tests. Results which obtained were as follows. Sera from 280 cattle(92.1%) contained antibodies against BRV which rate was the highest among the 8 viruses, and serum antibodies against BCV, BVDV, BIV, BAKV, BEFV, IBRV and PI-3V were detected from 266(87.5%), 149(49%), 108(35.5%), 94(30.9%), 80(26.3%), 32(10.5%) and 24(7.9%) cattle, respectively. Prevalence of seropositives to BVDV, BIV, BAKV, BEFV were higher among Holsteins than among the Korean native cattle(P<0.05). Prevalence of antibody titers against BVDV, BIV and BEFV in Korean native cattle were higher among females than males, while more males contained antibodies to BAKV, IBRV and PI-3V than females in their blood(P<0.05). Seropositives to BVDV, BIV, BAKV, BEFV and IBRV in Holsteins were higher among females than males(P<0.05). In Korean native cattle, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:32 and 1:2~1:64, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$. In Holsteins, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:64 and 1:2~1:32, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.11b
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• pp.983-986
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• 2003

현재의 가전 시장은 네트워크를 기반으로 갖춘 지능적인 인터넷 정보가전으로 발전되고 있다. 또한 휴대폰, PDA 와 같은 모바일 단말기를 이용하여 정보가전 기기들을 원격에서 제어하고자 하는 요구가 대두되고 있다. 본 논문에서는 휴대폰을 이용하여 댁내 외에서 댁내 정보가전 기기들을 모니터링하고 제어할 수 있는 효율적인 정보가전 제어 인터페이스를 설계한다. 휴대폰의 제한된 리소스를 고려하여 정보가전 제어를 위한 메뉴 구성과 사용자 인증 방식을 설계 구현한다.

• Journal of the korean veterinary medical association
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• v.28 no.5
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• pp.257-260
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• 1992

• Korean Journal of Plant Resources
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• v.27 no.3
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• pp.242-248
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• 2014

This study was conducted to establish an efficient transformation system by using somatic embryogenesis in an important Korean native conifer, Korean fir (Abies koreana). Embryogenic masses were induced from mature zygotic embryos of the Korean fir on Schenk and Hildebrandt medium, which was supplemented with thidiazuron. For genetic transformation, the embryogenic masses were co-cultivated with a disarmed Agrobacterium tumefaciens strain C58/pMP90 containing the plasmid vector pBIV10 or LBA4404 containing the plasmid vector MP90. Both vectors contain the kanamycin resistance and beta-glucuronidase (GUS) reporter genes. A total of 48 lines of embryogenic masses were selected on mLV medium containing $50{\mu}g/mL$ of kanamycin after 4 weeks of culture, following 3 days of co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 (none of the lines was cultivated with strain LBA4404 carrying MP90). Quantitative real-time PCR was performed, and high levels of GUS transcripts were observed in the 48 putative transgenic lines; however, the control (non-transgenic line) showed negative results. Results of histochemical staining showed that the expression of the GUS reporter gene was observed in somatic embryos that developed from the embryogenic masses of all 48 lines. Stably transformed cultures were successfully produced by co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 in Korean fir. Here, we have reported an Agrobacterium-mediated gene transfer protocol via somatic embryogenesis that may be helpful in developing breeding and conservation strategies for the Korean fir.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.511-511
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• 2017

하천에서 홍수 예방 및 가뭄재해 방지를 목적으로 최근 4대강 살리기 사업이 수행되었다. 특히, 안정적인 용수공급 및 다양한 수재해 피해를 최소화하기위해 다기능보가 설치되었다. 설치된 보는 고정보와 가동보로 구분되며 평상시에는 고정보를 넘어가는 월류흐름이 발생하며 홍수 시에는 추가적으로 가동보를 개방하여 오리피스 흐름이 발생한다. 최근 기후변화로 인해 집중호우가 빈번히 발생하며 이는 보 주변에서 빠르고 복잡한 흐름을 유발하게 된다. 이러한 흐름특성은 보하류의 세굴문제뿐만 아니라 물받이와 바닥보호공의 안전성에 영향을 미칠 수 있다. 본 연구에서는 영상기법을 활용하여 도수가 발생하는 조건에서 고정보 하류의 물받이공 및 바닥보호공에 난류흐름특성 및 압력분포를 분석하였다. 길이 8.0 m, 폭 0.3 m를 갖는 개수로에서 실험을 수행하였으며 0.2 m 높이의 고정보는 상류경계로부터 3.0 m지점에 위치하였다. 보 하류부에서 유속성분은 비접촉식 유속측정 기법인 입자영상유속계(Particle Image velocimetry, PIV)와 기포흐름을 측정할 수 있는 기포영상유속계(Bubble Image velocimetry, BIV)를 적용하였다. 그러므로 기포 존재유무와 관계없이 보 하류부 구간에 대한 유속장 성분을 취득하였다. 도수흐름은 물받이공 및 바닥보호공 영역의 바닥면을 따라 빠른 유속을 갖는 제트류가 발생하고 수면과 바닥으로 강한 진동이 발생하여 불안정한 흐름이 야기된다. 바닥면에 작용하는 압력을 측정하기 위해 흐름방향을 따라 피에조미터를 설치하였고 압력수두 분포를 초고속카메라로 촬영하여 그 특성을 분석하였다. 실험을 결과를 통해 보 하류부에 영향을 미치는 인자를 도출하였으며 향후 보 설계기준에 반영할 수 있을 것으로 기대된다.

• Economic and Environmental Geology
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• v.14 no.2
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• pp.77-91
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• 1981

An airborne magnetometer survey was carried out over an offshore area of about $200,000km^2$ from the southeastern, southern and western part of Korea. Detailed magnetic studies on the geological structure of the southern part of above area ($100,000km^2$) was accomplished. Residual aeromagnetic map was made in order to delineate magnetic provinces, magnetic lineaments and sedimentary basins by application of least square method using computer system. To determine the depth of the sedimentary basins pseudo-gravimetric method was applied. 1. The area studied is divided into four magnetic provinces for the purpose of interpretation on the basis of the magnetic maps. 2. Near shore area and its attached islands of southern part (fiirst and second magnetic province) can be regarded as being the extension from the land geology due to presentation of strong magnetic anomalies and shallow magnetic basements. 3. Magnetic lineament 1-1 is strong magnetic anomalous region which is presumably relevant to volcanic activities in Cretaceous. The depth of magnetic basement of the lineament was determined to 1,500 m. Negative magnetic anomalous zones B1-1 and B1-2 which represent Tertiary basins showed depth of magnetic basement 3 km and 4 km each. The latter can be interpreted as extension of the Taiwan basin which is consisted of Tertiary sediments. 4. Magnetic lineament 2-1 coincide with Rainan-Fukien massif running NE-SW direction. A lineament located in central part of magnetic lineament 2-1 is well connected with extension of Sobacksan anticlinal axis on land. Volcanic rocks in Gyongsang system concentrated along this lineament. 5. The characteristics of magnetic pattern in the southern Yellow sea basin of western part of Jeju island show weaker magnetic anomalies and deeper magnetic basements than first and second magnetic provinces indicating geological structure of this basin seems to be quite different from that of Jeju strait. 6. In southern part of Jeju island, smoother magnetic pattern develope southward. Maximum depth of magnetic basement in sedimentary basins BIV-1 and BIV-2 were determined down to 6,000 m increasing its thickness toward Taiwan up to 11,000 m in the shelf area off Taichung, Taiwan. Judging from the fact that hydrocarbon was founded in the Tertiary sediments of western coastal area of Taiwan, it can be expected that hydrocarbon will be existed in these sedimentary basins of southern part of Jeju island.

• Journal of Ocean Engineering and Technology
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• v.32 no.2
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• pp.77-83
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• 2018

In this study, a series of experiments were performed to investigate the variations in the behavior of green water generation and the flow kinematics of bubbly flow on deck with various flare angles. The experiments were conducted in a 2-D wave flume using a simplified model of a BW Pioneer FPSO operating in the Gulf of Mexico, with a 100-year return period wave condition. The green water phenomena were captured with a high speed CCD camera. The variations in the behavior of the green water generation were investigated with various flare angles, and the horizontal mean velocity profiles of bubbly flow on deck obtained using bubble image velocimetry (BIV) were provided. The differences in flow kinematics of bubbly flow on deck were analyzed with various flare angles.