• Journal of Convergence for Information Technology
• /
• v.12 no.3
• /
• pp.132-140
• /
• 2022

This study was conducted to identify the optimal lotion manufacturing conditions with decursin and decursinol angelate of Angelica gigas Nakai extraction. Lotion was confirmed that it had viscosity (5,208±112 cPs), assay (99.71±1.01%), and pH (5.62) for 3 months. The optimization of manufacturing conditions of mixing 4 for lotion formulation were made by 22+3 full factorial design. Mixing temperature (40-80℃) and mixing time (10-30 min) were used as independent variables with three responses(assay, pH, and weight variation) as critical quality attributes (CQAs). The model for assay and weight variation identified a proper fit having a determination coefficient of the regression equation (about 0.9) and a p-value less than 0.05. Estimated conditions for the optimal manufacturing process of lotion were 61.93℃ in mixing temperature and 15.85 min in mixing time. Predicted values at the mixing temperature (60℃) and mixing time (20 min) were 100.69% of assay, 5.57 of pH, and 98.07% of weight variation. In the verification of the actual measurement the obtained values showed 100.29±0.98% of assay, 5.57±0.02 of pH, and 98.27±0.89% of weight variation, respectively, in good agreement with predicted values.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.20 no.2
• /
• pp.5-11
• /
• 2018

There is a need to breed and distribute domestic varieties for Lilium Asiatic hybrids because most of the lily bulbs cultivated in Korea imported from foreign country. The genetic resources of Lilium Asiatic hybrids, 'Buff Pixie', 'Orange Matrix' and 'Tiny Puppet', which were collected from 2001, had been self-pollinated from 2004 to 2012. In 2012, Lilium Asiatic 'Lovely LiASong' as an F₁ hybrid was bred through crossing 'L₂-12-1' and 'L₂-35-4' obtained by 4th self-fertilization. 'Lovely LiASong' had a bowl shaped single flower with a few spots and medium-sized flower, 8.7cm in flower diameter. The number of flowers per plant was 4.9, which was relatively more flowers than other cultivars. And the flower inflorescence had umbellate and racemose while the attitude of longitudinal axis was erect. The plant height was 33.4cm with 30.4 leaves per plant and 7.2cm in leaf length. The 'Lovely LiASong' showed a high consumer preference because it had a relatively longer flowering period, larger flower diameter and number of flowers than those of the control, 'Tiny Ghost'.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
• /
• v.8 no.6
• /
• pp.957-966
• /
• 2018

Recently, the demand for remote patient monitoring based on IoT has been increased due to aging population and an increase in single-person household. A non-contact biological signal measurement system using multiple IR-UWB radars for remote patient monitoring is proposed in this paper. To reduce error signals, a multilayer Subtraction algorithm is applied because when the background subtraction algorithm was applied to the biological signal processing, errors occurred such as voltage noise and staircase phenomenon. Therefore, a multilayer background subtraction algorithm is applied to reduce error occurrence. The multilayer background subtraction algorithm extracts the signal by calculating the amount of change between the previous clutter and the current clutter. In this study, the SVD algorithm is used. We applied the improved multilayer background subtraction algorithm to biological signal measurement and computed the respiration rate through Fast Fourier Transform (FFT). To verify the proposed system using IR-UWB radars and multilayer background subtraction algorithm, the respiration rate was measured. The validity of this study was verified by obtaining a precision of 97.36% as a result of a control experiment with Neulog's attachment type breathing apparatus. The implemented algorithm improves the inconvenience of the existing contact wearable method.

• Journal of Marine Life Science
• /
• v.3 no.2
• /
• pp.81-86
• /
• 2018

The purpose of this study is to determine the effects of vibration on primary (e.g. plasma cortisol), secondary (e.g. plasma glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), Na⁺, K⁺ and Cl^- and tertiary (e.g. mortality) stress responses in cultured eel, Anguilla japonica. For this purpose, three groups (including one control group and two stress groups) were set up. The control group was made exposed to vibration corresponding to 48 decibel (dB, V) (produced using electric vibrators) for 15 minutes per hour every day, and the two stress groups was made exposed to vibration corresponding to 58 and 68 dB (V) (produced using the same electric vibrators), equally, for 15 minutes per hour every day. Blood was sampled at day 0 (before starting vibration stress tests, BS), and days 1, 3, 5, 7, 9 and 11 (after starting vibration stress tests). As a result, plasma cortisol showed trend to continuously rise by consecutive stress from 4.1±0.1 ng/ml in BS. In 48 dB group (control), cortisol showed the highest level with 7.6±0.9 ng/ml after 7 days (p<0.05), but at 9 and 11 days was not significantly compared with BS level. In 58 dB group, the cortisol showed the highest level with 43.1±4.8 ng/ml after 1st day. Cortisol of 68 dB group increased significantly during the experimental period (14.4±2.3~32.0±5.7 ng/ml) (p<0.05). In 58, and 68 dB groups during the experimental period differed significantly compared to 48 group (p<0.05). Glucose in 48 dB were increased from 42.0 ±5.7 (BS) to 52.5±2.1 (1 day), the level was not significantly from 1 to 11 days. Glucose in 58 and 68 dB groups was increased significantly than BS during experimental period (p<0.05). K⁺ in 68 dB increased significantly (p<0.05) from 2.3±0.2 mE/ql (BS) to 3.3±0.5 mE/ql at 5 days. In 48 and 58 dB groups during the experimental period differed significantly (p<0.05). Na⁺ and Cl^- levels were not differed significantly during the experimental period. AST and ALT in 58 and 68 dB groups showed trend to continuously rise by consecutive stress. At 7 and 9 days in AST, between 48, 58 and 68 dB groups differed significantly (p<0.05). In 48, 58 and 68 dB groups at 1 day, blood hematocrit increased significantly higher than BS. The 11 days after vibration stress, the mortality in 48, 58 and 68 dB groups was 1.1, 5.1 and 5.8%, respectively. The present results have shown that A. japonica exhibited ''typical'' physiological responses when exposed to chronic vibration stress. These data suggested that chronic vibration stress caused substantial stress in the fish; especially the persisting elevated plasma AST and ALT levels observed would be expected to adverse effect. In conclusion, chronic vibration stress could greatly affects the hematological characteristics in A. japonica.

• Journal of Marine Life Science
• /
• v.3 no.2
• /
• pp.96-100
• /
• 2018

Clonorchis sinensis is a main parasite that infects humans by making freshwater fish as an intermediate host in South Korea. There are so many reports about the infestation status of Clonorchis sinensis metacercariae (CsMc) in freshwater fish living in the river, but there are a few studies of fish in the lake. In this study, we examined CsMc in Cyprinid fish sampled from Soyang Lake, Namyang Lake, Andong Lake and Chunchun Lake from 2016 to 2017. Metacercaria of trematodes were found from Hemiculter eigenmanni and Carassius auratus in Namyang Lake, and Zacco platypus and Opsarichthys uncirostris in Soyang Lake. As a result of PCR using Clonorchis sinensis specific primer sets, it was confirmed that the metacercariae from Hemiculter eigenmanni in Namyang Lake was CsMc. This study provides information on CsMc infestation status of Cyprinid fish in four lakes and it is the first report of CsMc infestation in Namyang Lake.

• Journal of Marine Life Science
• /
• v.1 no.2
• /
• pp.109-116
• /
• 2016

We determined the effects of different water temperatures (15, 20, and 25℃) and photoperiod cycles (24L:0D, 12L:12D, and 0L:24D) on the oxygen consumption of the offspring of a cultured Japanese strain (JJ), a selected Korean strain (KK), and intraspecific hybrid strains (JK and KJ) of red seabream, Pagrus major, under starvation conditions. The different fish strains, water temperatures, and photoperiod cycles had effects on the mean oxygen consumption of fish. Oxygen consumption increased with increasing water temperatures for all photoperiod treatments (p<0.001). Fish held in continuous darkness (0L:24D) used consistently less oxygen than fish exposed to continuous light (p<0.05). The oxygen consumption of fish exposed to the light phase in a 12L:12D photoperiod was higher than that of fish in the dark phase of the 12L:12D cycle, and differences were significant in three of the strains: JJ (15℃), KK (15 and 20℃), and KJ (25℃). The oxygen consumption of the inbred (JJ and KK) and intraspecific hybrid (JK and KJ) strains varied with differing water temperatures and photoperiod cycles. The JK strain displayed significantly higher oxygen consumption than the other strains under all experimental conditions except 15℃ with a 0L:24D photoperiod. The JK and KJ strains usually showed the highest and lowest oxygen consumption values, respectively, whereas the inbred strains exhibited intermediate values. Oxygen consumption in the JJ and JK strains was usually higher than that of the KK and KJ strains. We propose that differences in the thermal sensitivity and photosensitization properties of the strains contribute to differences in their ability to adapt to changes in water temperature and photoperiod, thus resulting in differences in the amplitude of their metabolic rates.

• Journal of Marine Life Science
• /
• v.3 no.2
• /
• pp.51-58
• /
• 2018

Variations in water temperature are known to affect almost every part of fish physiology. The rise in water temperature due to climate change can physically damage fish. This study was conducted to evaluate the health status of the Atlantic salmon (Salmo salar) at high water temperature (20℃) than the optimum water temperature (15℃). Liver tissue exerts important metabolic functions in thermal adaptation. Therefore, liver tissue was used in this study. The evaluation method is to develop the biomarker gene using NGS RNAseq analysis and to examine the expression pattern using RT-qPCR analysis. The NGS RNAseq analysis revealed 1,366 differentially expressed genes, among which 880 genes were increase expressed and 486 genes were decrease expressed. The biomarker genes are such as heat shock protein 90 alpha (Hsp90α), heat shock protein 90 beta (Hsp90β) and cytochrome P450 1A (CYP1A). The selected genes are sensitive to changes in water temperature through NGS RNAseq analysis. Expression patterns of these genes through RT-qPCR were similar to those of NGS RNAseq analysis. The results of this study can be applied to other fish species and it is considered to be useful industrially.

• Journal of Marine Life Science
• /
• v.3 no.2
• /
• pp.59-66
• /
• 2018

The assessment of level of health of the tidal flats can be evaluate by health of organisms inhabit the tidal flats. It is possible to evaluate the precise health level of organisms inhabit the tidal flats using analysis of expression of biomarker genes. The purpose of this research is to evaluate the health of the tidal flats on the west coast using biomarker genes such as heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), glutathione S-transferases (GST) and thioredoxin (TRX). These genes are stress, immune, and antioxidant related genes that can be used to look at the health of an organism through gene expression. In this study, we collected manila clam (Ruditapes philippinarum) in 8 analysis areas on the west coast. Expression of the genes was analyzed by RT-qPCR method. Results showed that, the expression of Hsp70, Hsp90, GST and TRX genes were differentially expressed in the 8 analysis areas. In particular, the expression of Hsp90 and GST or the expression of Hsp70 and TRX were similar. This means that there is a substance that reacts specifically to each gene. Therefore, I think suggest that the based on the results of physicochemical analysis, it can be selected genes suitable for analysis. These results suggest that Hsp70, Hsp90, GST and TRX were played roles in biomarker for assessment of the health of tidal flats.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.30 no.1B
• /
• pp.101-105
• /
• 2010

Sunflower (Sunking4505) and Rape (Sunmang) are oil-seeds containing high oleic acid, and these are used for the production materials of bio-diesel and applying for phytoremediation. The effect of cadmium on germination rate and the growth of the plants is evaluated. Object seeds were placed in Cd (0, 1.5, 4, 12, 30, 60, 100, 150, 300, 500 mg/L) solutions for seven days, and germination rate, root length, shoot length, seedling length, and dry weight were observed. $IC_{50}$, seedling vigor index, and tolerance indices were computed, and data were statistically analyzed by Analysis of Variance (ANOVA). Germination rate as well as root, shoot, and seedling length decreased as the cadmium concentration increased except dry weight. The $IC_{50}$ of sunflower and rape are 112 and 10 mg-Cd/L, respectively. Only one of the sunflower seeds is germinated at 500 mg-Cd/L whereas rape seeds are not germinated more than 150 mg-Cd/L solution. Root has higher cadmium sensitivity than shoot, and sunflower has higher germination rate, growth, and seedling vigor index than rape. In case of tolerance indices, sunflower has lower value than rape at relatively low concentration, but has higher value at high concentration.

• Korean Journal of Environmental Agriculture
• /
• v.42 no.2
• /
• pp.112-120
• /
• 2023

Methane (CH₄) and nitrous oxide (N₂O) are significant contributors to greenhouse gas (GHG) emissions from rice fields. Mid-summer drainage is a commonly practiced water management technique that reduces CH₄ emissions from rice fields. Slow-release fertilizers gradually release nutrients over an extended period and have been shown to reduce N₂O emissions. However, the combined effect of slow-release fertilizer and water management on GHG emissions remains unclear. This study compared GHG emissions from a rice paddy subjected to mid-summer drainage for 10 days (control) with that of a rice paddy subjected to prolonged mid-summer drainage for 20 days combined with slow-release fertilizer (W+S). Gas sampling was conducted weekly using a closed chamber method. During the rice cultivation period, cumulative CH₄ and N₂O emissions were reduced by 12.3% and 16.2%, respectively, in the W+S treatment compared to the control. Moreover, the W+S treatment exhibited a 1.9% increase in grain yield compared to the control. Under experimental conditions, slow-release fertilizers, in combination with prolonged mid-summer drainage, proved to be the optimal approach for achieving high crop yield while reducing GHG emissions. This represents an effective strategy to mitigate GHG emissions from rice paddy fields.