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Extraction Yields of Hizikia fusiforme and Aloe vera Linne by Supercritical Carbon Dioxide and Antimicrobial Activity of their Extracts (초임계이산화탄소에 의한 톳과 알로에 추출물의 수율 및 항균활성)

  • Lim, Sang-Bin;Kim, Soo-Hyun;Ko, Young-Hwan;Oh, Chang-Kyung;Oh, Myeong-Cheol;Ko, Yong-Gu;Park, Che-Seok
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.68-73
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    • 1995
  • Extraction yields of Hizikia fusiforme and Aloe vera Linne by supercritical carbon dioxide($SC-CO_{2}$) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Alternaria sp. were determined. Both yield and solubility of the extracts from Hizikia fusiforme and Aloe vera Linne by $SC-CO_{2}$ with ethanol were two times greater than those by only $SC-CO_{2}$. All of the extracts demonstrated antimicrobial activities in the decreasing order of bacteria, yeast and fungus. The extracts by $SC-CO_{2}$ with ethanol showed almost the same degree of microbial growth inhibition as those by only $SC-CO_{2}$. Based upon these data, it was speculated that the components soluble in nonpolar solvent might be more responsible for the antimicrobial activity.

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Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

  • Muku, Rosline James;Yan, Hong-Bin;Ohiolei, John Asekhaen;Saaid, Abubakar Ahmed;Ahmed, Sara;Jia, Wan-Zhong;Fu, Bao-Quan
    • Parasites, Hosts and Diseases
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    • v.58 no.1
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    • pp.93-97
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    • 2020
  • The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to T. hydatigena has large veterinary and economic drawbacks. Like other taeniids, e.g., Echinococcus, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of T. hydatigena, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) mitochondrial genes. The isolates were correctly identified as T. hydatigena with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the nad1 and cox1 genes, respectively. The results suggest the presence of unique T. hydatigena haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of T. hydatigena in Africa, this report represents the first insights into the genetic variation of T. hydatigena in Sudan and constitutes useful data.

Genetic parameter estimation for reproductive traits in QingYu pigs and comparison of carcass and meat quality traits to Berkshire×QingYu crossbred pigs

  • Luo, Jia;Yang, Yiting;Liao, Kun;Liu, Bin;Chen, Ying;Shen, Linyuan;Chen, Lei;Jiang, An'an;Liu, Yihui;Li, Qiang;Wang, Jinyong;Li, Xuewei;Zhang, Shunhua;Zhu, Li
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.8
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    • pp.1224-1232
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    • 2020
  • Objective: The QingYu pig is well known for its excellent meat quality attributes in Sichuan province, China. In order to improve its production efficiency, the determination of genetic factors contributing to quantifiable economic traits of livestock is important. Moreover, the cross-breeding of QingYu pigs with western breeds possessing strong growth attributes is an efficient way to improve the performance of this breed. Methods: Here, the genetic parameters of several important reproductive traits of QingYu pigs were estimated, include total number born (TNB), number born alive, litter birth weight, individual birth weight, number of piglets weaned, litter weaning weight, and individual weaning weight. The data was analyzed using the ASReml 3.0 software (NSW Inc., Sydney, Australia). Furthermore, the effects of crossing Berkshire with QingYu (BQ) pigs on carcass and meat quality traits, as well as the effects of slaughter weight on carcass and meat quality of BQ were characterized. Results: QingYu pigs exhibited superior reproductive traits. The TNB available to QingYu pigs was more than 8 per parity. The observed repeatability of the reproductive traits of the QingYu pigs was between 0.10 and 0.23. The significantly correlated genetic and phenotypic of reproduction traits were consistent. Interestingly, the BQ pigs exhibited improved carcass quality, with a significant increase in loin muscle area, lean percentage and reduction in sebum percentage. As a result, BQ had higher L45min, lower cooking scores, and lower drip loss. In addition, the loin muscle area, body length, and sebum percentage were significantly higher in 90 and 100 kg animals. Cooking loss showed a significant increase at 80 kg, and marbling increased significantly from 90 kg. Conclusion: The results of this study suggest that QingYu pigs exhibit excellent reproductive properties and heritability of these traits. Crossing with Berkshire is an efficient strategy to improve the carcass and meat quality of QingYu pigs for commercial operations. Furthermore, it appears as though the optimal slaughter weight of BQ pigs is at approximately 90 kg.

Classification and Profiling of Bus Stops in Gyeong-gi Province on the Basis of Trip Chain Variables (통행연계 변수를 중심으로 한 경기도 버스정류장 유형 구분)

  • Bin, Mi-Young;Jung, Eui-Seok;Lee, Won-Do;Joh, Chang-Hyeon
    • Journal of the Economic Geographical Society of Korea
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    • v.15 no.2
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    • pp.332-342
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    • 2012
  • The current research aims at classifying the bus stops as transfer center in order to establish the rational bus transfer systems. Existing research typically identifies characteristics of demands for bus stops and land use surrounding the bus stops and classifies and profiles the bus stops. A common problem with this type of research is that the results with cross-sectional characteristics of land use and bus stop usage do not capture the details of trip chain, the fundamental characteristics of the trips with transfer. This paper therefore examines bus stop classifications with such variables as transport mode chains, intermediate stop chains and timing chains. The analysis on the data collected on Monday 20 April 2009 for passengers of Gyeong-gi bus results in a clear classification among bus stops in terms of such trip chain variables. The research would provide useful information for the decision support of transfer stops location choice and infrastructure design.

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A Study on the Verification of Design Compatibility for a Europe Type Automatic Coupler Head according to TSI Certification Standards (TSI 인증기준에 따른 유럽 표준형 자동복합연결기 헤드의 설계적합성 검증 연구)

  • Min, Kyeong Bin;Park, Jin Kyu;Kang, Ji Sung;Kim, Ki Nam
    • Journal of the Korean Society for Railway
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    • v.19 no.1
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    • pp.29-37
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    • 2016
  • The type approval system of the railway safety law, which was enforced in 2014, has been preferentially applied to the field of railway vehicles. The type approval system addresses railway vehicles and railway constituents. Unlike the previous system, it requires serial verification stages divided, in consecutive order, into verification of design compatibility and conformity and type test. These stages are in accord with international certification systems including TSI certification of the European commission. This study has been carried out to find and meet the design requirements for a coupler head, which is a part of the automatic coupler system; requirements were drawn from TSI and technical specifications that are subordinate to the domestic railway approval system. Through this study, it has been found that there exist 34 design requirements to acquire a complete coupler head. Among those requirements, 32 were verified by document inspection and two were able to be verified by performing FEA and gathering range analysis data.

Ginsenoside compound-Mc1 attenuates oxidative stress and apoptosis in cardiomyocytes through an AMP-activated protein kinase-dependent mechanism

  • Hong, So-hyeon;Hwang, Hwan-Jin;Kim, Joo Won;Kim, Jung A.;Lee, You Bin;Roh, Eun;Choi, Kyung Mook;Baik, Sei Hyun;Yoo, Hye Jin
    • Journal of Ginseng Research
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    • v.44 no.4
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    • pp.664-671
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    • 2020
  • Background: Ginsenoside compound-Mc1 (Mc1) is a member of the deglycosylated ginsenosides obtained from ginseng extract. Although several ginsenosides have a cardioprotective effect, this has not been demonstrated in ginsenoside Mc1. Methods: We treated H9c2 cells with hydrogen peroxide (H2O2) and ginsenoside Mc1 to evaluate the antioxidant effects of Mc1. The levels of antioxidant molecules, catalase, and superoxide dismutase 2 (SOD2) were measured, and cell viability was determined using the Bcl2-associated X protein (Bax):B-cell lymphoma-extra large ratio, a cytotoxicity assay, and flow cytometry. We generated mice with high-fat diet (HFD)-induced obesity using ginsenoside Mc1 and assessed their heart tissues to evaluate the antioxidant effect and the fibrosis-reducing capability of ginsenoside Mc1. Results: Ginsenoside Mc1 significantly increased the level of phosphorylated AMP-activated protein kinase (AMPK) in the H9c2 cells. The expression levels of catalase and SOD2 increased significantly after treatment with ginsenoside Mc1, resulting in a decrease in the production of H2O2-mediated reactive oxygen species. Treatment with ginsenoside Mc1 also significantly reduced the H2O2-mediated elevation of the Bax:Bcl2 ratio and the number of DNA-damaged cells, which was significantly attenuated by treatment with an AMPK inhibitor. Consistent with the in vitro data, ginsenoside Mc1 upregulated the levels of catalase and SOD2 and decreased the Bax:B-cell lymphoma-extra large ratio and caspase-3 activity in the heart tissues of HFD-induced obese mice, resulting in reduced collagen deposition. Conclusion: Ginsenoside Mc1 decreases oxidative stress and increases cell viability in H9c2 cells and the heart tissue isolated from HFD-fed mice via an AMPK-dependent mechanism, suggesting its potential as a novel therapeutic agent for oxidative stress-related cardiac diseases.

The Clinical Analysis of 100 cases of Coronary artery Bypass Grafting with the Right Gastroepiploic artery (우위대망동맥을 이용한 관상동맥 우회술 100례의 임상적 고찰)

  • Song, Hyun;Lim, Han-Jung;Lee, Hyun-Woo;Jung, Jong-Pil;Shin, Je-Kyoun;Kim, Jong-Ook;Park, Jong-Bin;Lee, Jae-Won;Song, Meong-Gun
    • Journal of Chest Surgery
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    • v.33 no.8
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    • pp.638-642
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    • 2000
  • Background: In an effort t enhance long term patency of coronary bypass grafts, utilization of arterial conduits have been on an icrease. With the same objective, we have been using the right gastroepiploic artery(RGEA)in coronary artery bypass procedures since 1998. The current paper has been undertaken with the aim of assessing the apropriateness, problems, and short term results of using the RGEA as an arterial graft conduit by studying the postoperative clinical results of 100 patients than received coronary artery bypass grafting (CARG) with this artery. Material and Method: Between May of 1998 and May of 1999, an analysis of the mortality, postoperative myocardial infarction, and the need for IABP insertion as a result of low cardiac output were made between 100 consecutive patients undergoing CABG with the RGEA. Result: There was one postoperative death due to cerebral infarction. Postoperative complications/morbidity comprised myocardial infarction in 2, cerebral infarct in 3, reoperation due to bleeding in 1, mediastinitis in 1, and low cardiac output syndrome necessitating IABP in 3 patients. Complicatons related to harvesting of the arterial grafts were not experienced in any of the patients. Conclusion: The results of the current data show that utilization of the RGEA in CABG is not associated with increased mortality/morbidity and demonstrates satisfactory short term results suggesting the usefulnessof this conduit as an arterial graft.

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Transitions of Urban Parks in Busan noticed by the Chosun Planning Ordinance in the Japanese Colonial Period (일제강점기 조선시가지계획령에 고시된 부산 소재 도시공원의 변천)

  • Kim, Yeong-Ha;Yoon, Guk-Bin;Kang, Young-Jo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.1
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    • pp.1-15
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    • 2017
  • This study investigated the process of change of 32 urban parks designated during the period of Japanese colonial rule according to growth and development of the city in Busan. Particularly, researching included analyizing books, notification, data, and documents relevant to the history of urban planning published by Busan city. As a result, Daejeong Park, Gokwan Park, and Yongdusan Park had been utilized by Japanese residents under the Japanese colonial rule before planning of urban parks, and 32 parks legally specified in 1944 were planned by considering the prevention against disasters. After emancipation, there were an unauthorized building, housing construction, business district, public office, and school facility in the sites of the parks due to the influence of the Korean War and reorganization of urban planning. The majority of parkways and small parks downtown were eliminated. However, unexecuted parks that the government had designed on the edge of town during the Japanese colonial period have become major parks downtown through the city's growth. Yeonji Park, Yangjeong Park, and Danggok Park have been being building as a business of parks for a comfortable city, forming downtown along with the Green-Busan Policy. Thus, 32 parks designated under the Japanese colonial rule have made or got out of use reflecting on the phases of the times of modern Korean society. It turns out that these parks need an investigation about condition for land possession and purchase of the site of the parks in order for social common capital.

Study of Quality Change in Chonggak-kimchi during Storage, for Development of a Freshness Indicator (신선도 표시계 개발을 위한 총각김치의 저장 중 품질변화 척도)

  • Kim, Yun-Jung;Jin, You-Young;Song, Kyung-Bin
    • Food Science and Preservation
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    • v.15 no.4
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    • pp.491-496
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    • 2008
  • To develop a freshness indicator of Chonggak-kimchi for marketing purposes, Chonggak-kimchi was prepared and pH, total acidity, total aerobic bacterial load, lactic acid bacterial levels, and reducing sugar content were measured. Sensory evaluation tests on product stored at $4^{\circ}C,\;10^{\circ}C,\;and\;20^{\circ}C$, were performed. The pH increased slightly early in storage, and then decreased to pH 4.2-4.3 for all samples. The rate of decrease of pH rose with increasing storage temperature. Total acidity values rapidly increased after 1, 5, and 7 days of storage at $20^{\circ}C,\;10^{\circ}C,\;and\;4^{\circ}C$, respectively. Populations of total aerobic bacteria and lactic acid bacteria increased slightly until 4 days, 10 days, and 30 days of storage at $20^{\circ}C,\;10^{\circ}C,\;and\;4^{\circ}C$, respectively, and then decreased drastically. Sensory evaluation data showed that Chonggak-kimchi was edible until 4 days(pH 4.5), 10 days(pH 4.4), and 30 days(pH 4.3) of storage at $20^{\circ}C,\;10^{\circ}C,\;and\;4^{\circ}C$, respectively. These results clearly suggest that the shelf-life of Chonggak-kimchi depends on storage temperature, and the pH limit for marketing is 4.3; this is a freshness indicator for Chonggak-kimchi.

Weed Flora of Arable Peat in Selangor, Malaysia - Quantitative and Spatial Pattern Analyses (말레이지아 세랑고지역 부식질토양경지 잡초식생의 정량생태분석)

  • Bakar, Baki Bin;Wong Nyuk Yin, Fenny;Kwon, Yong-Woong
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.382-389
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    • 1997
  • Collated data from the 1995-1996 floristic surveys of weeds of arable peat in Selangor district were analysed to assess composition and dominance and spatial distribution pattern based on quantitative and dispersion indices. Forty eight weed species belonging to 19 families of which 31 were broad leaves, 10 grasses and 7 sedges were sampled and these ware translated as 77.8 and 15% of the total cover, respectively, The respective important values were 71.11 and 18%. Ten species in the onder of dominance were Fimbristylis acuminata, Murdannia nudiflora, Hedyotis corymbosa, Ageratum conyzoides, Asystasia gangetica, Cleome rutidosperma, Cyperus sphacelatus, Lindernia crustacea, Ludwigia hyssopifolia of spatial distribution based on variance-to-mean ratios, Llouds mean crowding or Lloyds patchiness indices. Other species were either random or regular in their spatial distribution. Differences in species-dominance and spatial distribution pattern may be attributed to inherent variations in patchiness and fecundity schedules of each weed species, crops, cropping patterns and agronomic practices prevailing in the area.

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