• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.893-897
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• 2002

The chloroform layer from 75% ethanol extract of Dalbergia odorifera T.CHEN showed strong antioxidative activity on lard and palm oil as tested by Rancimat method. Antioxidative active compound isolated and identified by silica gel column chromatography, thin layer chromatography, mass spectrophotometer, $^1H-NMR$ and $^{13}C-NMR$ was identified as mucronulatol (3(R&S)-3,7-Dihydroxy-2',4-dimethoxyisoflavan). Results of Rancimat method revealed the induction period of Mucronulatol increased longer than those of synthetic antioxidant, BHA and BHT, at the same concentration. Mucronulatol combined with ${\delta}-tocopherol(200ppm)$, and with ascorbic acid (200 ppm) and citric acid (200 ppm) on lard and palm oil, respectively, showed strong synergistic effects.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.395-399
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• 1997

Meat model emulsions ware prepared with salt-soluble protein and soybean oil. Effects of water-soluble protein (WSP) on the meat model emulsion treated with/without BHT during 8 day storage $5^{\circ}C$ under both dark and light illumination were studied by measuring POV and TBA. An emulsion without BHA and WSP was used as a control. Under light storage, there was no significant difference in peroxide values between the control and the sample treated with BHA except the 2nd day of storage. However, TBA values of the sample treated with BHA were significantly (p<0.05) lower than those of control except the 4th day of storage. TBA and POV of the samples treated with WSP and WSP + BHA were higher than control after 4th day of storage under light. That is, water soluble protein, which was composed mainly of myoglobin, increased lipid oxidation under light storage. The similar trends were also shown in the samples stored under dark. These results suggested that acceleration of lipid oxidation of the meat model emulsions by water soluble protein (WSP) under both light and dark might not be due to the singlet oxygen formation, but due to superoxide anion formed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.1
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• pp.1-7
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• 2008

The antioxidative, antimutagenic and cytotoxic activities of ethanol extract from Cornus officianalis have been studied. The antioxidant activity of the ethanol extract was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The inhibition effects on the mutagenicity in Salmonella Typhimurium TA100 were evaluated by Ames test and cancer cell inhibitory effects in Hep3B cell and HeLa cell were tested by MTT assay. Cornus officianalis had an important free radical-scavenging activity towards the DPPH radical. At a concentration of 500 ppm, the DPPH radical-scavenging activity of Cornus officianalis was similar to that of L-ascorbic acid. None of the extracts produced a mutagenic effect on S. Typhimurium TA100. The ethanol extract from Cornus officianalis showed about 77% of inhibition at 500 ppm on the mutagenicity induced by 4-nitroquinoline-1-oxide. The extract from Cornus officianalis showed strong cytotoxicity against Hep3B and HeLa cells, with inhibition of 83 and 78% at a dose of $700{\mu}g$/plate, respectively. Moreover, the ethanol extracts had 34.33 mg H.E/g of polyphenols and 5.67 mg Q.E/g of flavonoids, respectively. Therefore, the present study showed antioxidative, antimutagenic and anticancer potential of the ethanol extract from Cornus officianalis.

• Food Science and Preservation
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• v.15 no.3
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• pp.437-444
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• 2008

Structured lipids(SLs) were synthesized by enzymatic interesterification with DHA-enriched fish oil(containing 27% docosahexaenoic acid) and soybean oil in the hatch-type reactor. The interesterification was performed for 24 hr at $55^{\circ}C$ and TLIM(immobilized lipase from Thermonyces lanuginosa, 10% by weight of total substrates) was mixed with 180 rpm of shaking. The fish oil and soybean oil were interesterifed with several weight ratio(fish oil : soybean oil, 2:8, 3:7, 4:6, 5:5, w:w), Reverse-phase high performance liquid chromatography with an evaporative light-scattering detector separated the triglyceride species of SLs. The products contained the newly synthesized peaks. Especially, one of peaks was distinctively increased with the increasing weight ratio from 2:8 to 5:5 while the peak of trilinolein (LLL) decreased vice versa. The effect of antioxidants such as catechin, BHT(Butylated hydroxytoluene), and their combinations on the oxidative stability in SL were investigated. Oxidative stability was carried out under oven test at $60^{\circ}C$ over 72 hr thereafter SLs were analyzed for total fatty acid content, rancimat, peroxide value, electronic nose and TBARS value. Among all combinations of antioxidant, the highest stability was obtained from 200 ppm of catechin. Besides, total tocopherol ($\alpha$, $\gamma$, and $\delta$-tocopherol), iodine and saponification value were analyzed in which iodine and saponification value of SLs were 151.19 and 182.35.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.4 s.304
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• pp.49-59
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• 2005

This paper proposed a six-color separation using additional colorants and quantitative granularity metric to reduce color difference and graininess. In the conventional method, light magenta and light cyan are used in the bright region instead of magenta and cyan. However, the hue value of liBht magenta and light cyan is different from the one of magenta and cyan in CIELAB space, so that this makes the colorimetric reproduction more or less inaccurate. To improve this inaccuracy, the proposed method uses yellow and light magenta colorants as the additional colorants. In the bright region, magenta is replaced with light magenta and yellow, while cyan is replaced with light cyan and light magenta. This selection reduces hue difference because it creates the color of similar hue to magenta and cyan. In addition, smooth image can be simultaneously obtained by the less dot visibility of additional colorants. In the middle region, magenta is replaced with light magenta and magenta, while cyan is replaced with light cyan and cyan. The use of two colorants having a different concentration makes the dot Pattern coarse. To reflect this Phenomenon, quantitative granularity metric is used. In the dark region, only magenta and cyan colorant is used as usual. Through experiments, it is shown that the proposed method improves both colorimetric and smooth tone reproductions.

• Korean Journal of Food Science and Technology
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• v.35 no.2
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• pp.309-315
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• 2003

Nutritional composition, contents of phenolic compounds, and antioxidative activities of small black soybean were investigated. Proximate composition of the soybean was crude fat, crude protein, carbohydrate, and crude ash at 15.9, 34.7, 38.5, and 3.7%, respectively. Potassium was the predominant mineral, followed by phosphorus, calcium, and magnesium. Glucose and galactose were the major sugars. Twelve different kinds of fatty acids were identified in whole soybean, dehulled soybean, and soybean hull. The 75% methanol and ethyl acetate extract fractions from the soybean hull contained total phenolic compounds at 4.64 and 13.53 g/100 g, respectively. The methanol extract of soybean hull showed strongest antioxidative activity among samples tested. Ethyl acetate fraction of the soybean hull exhibited DPPH radical scavenging activity at level similar to those of ${\alpha}-tocopherol$ and BHT. Phenolic compounds were the major biological components in the soybean hull. These results suggest that small black soybean can be used as a new material for functional food.

• Journal of Life Science
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• v.26 no.8
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• pp.948-954
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• 2016

We investigated the flavonoid and phenol contents and antioxidant effect of wine by-product extract. Antioxidant effects were measured with 1,1-diphenyl-2-picryhydrazyl (DPPH) and 2.2'-azino-bis(3-ethylbenothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS+) assays. Cellular reactive oxygen species (ROS) were measured with the dichlorofluorescein-diacetate (DCFH-DA) assay. The flavonoid and phenol contents of the methanol (MeOH) extract were greater than those of the acetone+methylene chloride (A+M) extract. Among fractions, the 85% aqueous methanol (85% aq. MeOH) fraction contained the highest flavonoid contents, while the n-BuOH fraction had more phenol contents. In the DPPH and ABTS assays, the MeOH extract showed a scavenging effect greater than that of the A+M extract (p<0.05). The n-BuOH fraction (0.5 mg/ml concentrations) showed scavenging effects of 72% and 92%, respectively, in the DPPH and ABTS assays (p<0.05). However, the 85% aq. MeOH fraction showed a 90% scavenging effect in the DPPH assay only. In 120 min ROS production assay, all tested fractions dose-dependently decreased cellular ROS production induced by H₂O₂ in comparison with that produced by exposure to the extract-free control. The MeOH extract showed a higher sinhibitory effect on cellular ROS producing than that of the A+M extract at all concentrations tested. Treatment with the n-BuOH fraction (0.1 mg/ml concentrations) inhibited cellular ROS production by 60%. These results indicate that the n-BuOH fraction of wine by-product extract inhibited cellular oxidation and may contain valuable bioactive compounds such as flavonoids and phenols.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.1
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• pp.20-27
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• 2013

Proximate composition, total phenolics and total flavonoids level, DPPH radical scavenging activity, and cytotoxicity were determined in the methanol extracts of different plant parts of Youngia sonchifolia at reproductive growth stage. Crude protein and crude fat were present as the highest amount in flowers, and crude fiber in the stems and roots. The highest content of phenolics [mg ferulic acid equivalents (FAE) $kg^{-1}$ dry weight (DW)] was found in flowers (highest) and followed by leaves, stems and roots (lowest). Flavonoids [mg rutin equivalents $kg^{-1}$ DW] level, however, showed the highest in leaf extracts and lowest in root extracts. The antioxidant potential of the methanol extracts from the plants dose-dependently increased DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity (%). DPPH radical scavenging activity were highest in root extracts ($IC_{50}=1,135.6\;mg\;kg^{-1}$) and followed by leaf, stem and flower extracts. By way of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, methanol extracts of roots showed the highest anticancer activity on human cancer cell line Calu-6 for human pulmonary carcinoma ($IC_{50}=196.3\;mg\;kg^{-1}$) and HCT-116 for human colon carcinoma ($IC_{50}=623.6\;mg\;kg^{-1}$).

• Applied Biological Chemistry
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• v.51 no.1
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• pp.11-16
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• 2008

The extraction conditions for resveratrol production from grape fruit stem, which is a by-product of grape processing, were optimized to develop high-functional grape-based products. Additionally, the bioefficacy of grape fruit stem extract (GFSE) as an antioxidant agent was evaluated. Resveratrol was extracted using various experimental conditions such as extractant type, extractant concentration, raw material-extractant ratio, extraction time and temperature, and the results were analyzed using a statistical program (SPSS). The resveratrol yield was the highest when 80% ethanol with a raw-material-extractant ratio of 1:10 (w/v) was used. In addition, the optimal temperature and time were selected as $60^{\circ}C$ and 90 min, respectively. When the antioxidant activity was analyzed and expressed as DPPH radical scavenging activity and SOD-like activity, the antioxidant activity of GFSE was higher than that of BHT, BHA and L-ascorbic acid. Finally, it was found that GFSE could be used as a raw material for the production of high antioxidant agents.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2006.06a
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• pp.573-601
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• 2006

IS 실무자들은 과거처럼 단순한 시스템 분석이나 프로그래밍 기법만을 갖추는 것만으로는 조직이 원하는 정보기술을 효과적으로 운용하는 것이 어렵게 되고 있다. 예전과는 달리 최근의 기업에서는 통신 시스템을 포함하는 다양한 정보기술에 관련된 지식과 기술을 전문적으로 다룰 수 있는 전문가를 원하는 추세이다. 이러한 맥락에서 IS 실무자들이 자신의 업무를 성공적으로 수행하기 위해 필요한 전문 지식과 기술은 무엇인가라는 질문에 대한 대답을 알 수 있어야만 한다. 본 연구는 IS 실무자들이 그들이 직면하고 있는 ‘IS 지식과 기술의 빠른 변화’를 얼마만큼 인식하고 있으며, 그들이 필요로 하는 지식과 기술과 업무를 수행함에 있어 필수적인 지식과 기술을 얼마만 큼 보유하고 있는지를 조사하였다. 본 연구에서는 조사된 자료를 통하여, 기존의 국내외의 문화에서 밝혀진 인구 통계학적 분류기준 (예를 들자면, 경력 수준, 지역, 직종) 이외에 이들을 분류할 수 있는 기준에는 어떠한 것이 있는가에 대한 연구를 수행하였다. 분석을 위하여 실무자들이 현업에서 많은 시간과 노력을 들이고 있는 IS 활동영역에 대한 투자시간을 기준으로 실무자들을 분류하였다. 분석에서는 조사자의 군집분석과 다차원 분석을 통하여 분류된 실무자 그룹에 대한 여러 가지 기술적인 특성과, 인구 통계학적 특성을 파악하고, 그룹들에 대하여 새로운 분류에 적합한 표기를 제시하고자 하였다. 본 논문은 정보시스템 영역에서 수행된 IS 실무자들에 다양한 연구의 한 부분으로서, 기업 환경, 조직 환경, 나아가 실무자들의 직무환경의 개선에 필요한 지식과 기술을 제공할 것이다.아날로그 방식에서 IT 기반에 의한 디지털 환경으로 변화되고 있다. 또한 e러닝, T러닝, m러닝, u러닝 등의 용어가 생성되고 있다.키지에어컨에서 사용되고 있는 밀폐형 압축기에 대해서 그림 2에서 나타내고 있는 냉방능력 10tons(120,000Btu/h) 이하를 중심으로 상기의 최근 기술 동향을 간략하게 소개하고자 한다.질표준의 지표성분으로 간주되는 진세노사이드의 절대함량과 그 성분조성 차이에 따른 임상효과의 차별성이 있는지에 대한 검토와, 특히 최근 실험적으로 밝혀지고 있는 사포닌 성분의 장내 세균에 의한 생물전환체의 인체 실험을 통한 효과 검정이 필요하다. 나아가서는 적정 복용량의 설정과 이와 관련되는 생체내 동태 및 생체이용율(bioavilability)에 관한 정보가 거의 없으므로 이것도 금후 검토해야 할 과제로 사료된다. 인삼은 전통약물로서 오랜 역사성과 그동안의 연구결과에 의한 과학성을 가지고 있으므로 건강유지와 병의 예방 및 회복촉진을 위한 보조요법제 또는 기능성 식품으로써의 유용성이 있는 것으로 판단된다. 앞으로 인삼의 활용성 증대를 위해서는 보다 과학적인 임상평가에 의한 안전성 및 유효성 입증과 제품의 엄격한 품질관리의 필요성이 더욱 강조되어야 할 것이다.xyl radical 생성 억제 효과를 보여 주었다. 본 실험을 통하여 BHT 를 제외하고 전반적으로 세포 수준에서의 oxidative stress 에 대한 억제 효과를 확인해 볼 수 있었으며 특히 수용성 항산화제들에서 두드러진 효과를 보여 주었다. 제공하여 내수기반 확충에도 노력해야 할 것 이다.있었다., 인삼이 성장될 때 부분적인 영양상태의