• Reproductive and Developmental Biology
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• 제32권4호
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• pp.237-243
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• 2008

The objective of this study was to examine the effect of caffeine and sodium bicarbonate in a fertilization medium on the fertilizability of boar spermatozoa that were frozen in straws. Boar spermatozoa were extended with Beltsville F5 extender and frozen in 0.25-ml straws. In vitro matured porcine oocytes were fertilized in vitro (IVF) with frozen-thawed boar spermatozoa for 6h in a modified tris-buffered medium (mTBM) or in its modified medium by substituting the tris with 25mM sodium bicarbonate (modified bicarbonate-buffered medium; mBBM). Some of inseminated oocytes were fixed and stained for examination of sperm penetration. IVF embryos were cultured in a North Carolina State University-23 medium for embryo development. The percentage of live sperm was $47{\pm}4%$ and morphological abnormality of acrosome was found in $14{\pm}3%$ of spermatozoa. Optimal sperm concentration for IVF was $0.75{\sim}1.0{\times}1.0{\times}10^6$ sperms/ml when mTBM containing 5mM caffeine was used as the fertilization medium. Sperm penetration was significantly (p<0.05) stimulated by increasing caffeine concentration in the IVF medium. In addition, mBBM significantly (p<0.05) increased sperm penetration (92%) compared to mTBM (65%). More (p<0.05) blastocysts (22% vs. 32%) developed from the oocytes that were fertilized in mBBM containing 1mM caffeine than from those fertilized in mTBM with 5mM caffeine. Our results indicate that boar spermatozoa can be frozen successfully in straws with holding their normal fertilizability and that caffeine and sodium bicarbonate stimulates sperm penetration in vitro.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1659-1664
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• 2008

To study the working mechanisms for non-starch polysaccharidases to improve the growth performance of broiler chickens, a 21-day feeding trial was conducted. Two dietary treatments were included: 1) wheat diet (the control); 2) wheat+xylanase diet (xylanase, Allzyme PT, Alltech, Kentucky, USA). There were 8 replicates with 8 birds each for each treatment and the experimental diets were given to birds from hatch. Feed intake and body weight were measured on days 7 and 21. At the same ages, samples were taken for the determination of selected groups of luminal and mucosa-associated bacteria, mucosal morphology, brush-border membrane (BBM) bound enzyme activity and ileal nutrient digestibility. The xylanase supplement increased (p<0.05) body weight gain (BWG) and improved feed conversion ratio (FCR) at the end of the experiment but protein and starch digestibilities were not affected (p>0.05) by xylanase. Up to day 7, xylanase increased the counts of C. perfringens in the ileum and total anaerobic bacteria (TAB) in the caeca (p<0.05, p=0.07, respectively). By day 21, the counts of ileal lactobacilli (p<0.05) and TAB (p=0.07) were lower in birds given the xylanase-supplemented diet than in those on the control diet. No significant differences were observed in the counts of mucosa-associated lactobacilli and coliforms between xylanase treatment and the control at both ages. Villus height at the jejunum was not affected (p>0.05) by the supplement but crypt depth at the same site was reduced at day 7. Also, xylanase tended to increase the concentration of BBM protein (p = 0.09) and the specific activity of sucrase (p = 0.07) at day 21.

• 콘크리트학회논문집
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• 제15권2호
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• pp.288-296
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• 2003

최근 말뚝기초 공법으로서 강관말뚝의 활용이 토목 및 건축 분야에서 증대됨에 따라 이종의 재질을 가진 강관 말뚝과 콘크리트 확대 기초의 연결방법에 대한 기술개발 및 실제거동 파악에 대한 관심이 매우 높아지고 있다. 연결부에 대한 국내 시방서는 두 가지(A, B-type) 연결방법을 제시하고 있지만 실제적으로는 B-type 방법이 주로 활용되고 있는 상황이다. 본 논문에서는 강관 말뚝의 B-type 연결방법의 구조거동을 파악하기 위하여 말뚝에 작용하는 대표적인 하중인 연직하중에 대하여 3종류와 수평 하중에 대하여 두 종류의 실험체에 대한 재하시험을 실시하였다. 또한 재하실험 결과값에 대한 비교 분석을 위하여 3차원 유한 요소해석을 수행하였다. 실험과 수치해석 결과를 비교 분석한 바 현재 강관 말뚝 연결부의 설계법으로 적용되고 있는 가상 철근 콘크리트 기둥의 거동을 확인하였다. 또한 콘크리트 확대기초에 작용하는 하중을 강관으로 전달하기 위하여 사용되는 미끌림 방지턱의 역할과 이러한 방지턱이 강관과 콘크리트의 합성거동에 미치는 영향을 확인하였다. 본 연구를 통하여 향후 합리적인 강관 말뚝 연결부에 대한 설계 방법 및 기준을 정립함에 있어 기초 연구자료와 강관 말뚝 연결부의 구조거동을 파악하기 위한 실험방법을 제시하였다.

• 한국통신학회논문지
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• 제25권4B호
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• pp.725-733
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• 2000

본 논문에서는 MPEG-4의 텍스처 부호화시 변형된 경계 블록 병합 기법을 제안하였다. 경계 블록 병합 기법의 전처리 과정으로 VOP를 나누는 위치를 조절하여 병합 시 부호화해야 할 블록의 수가 최소가 되는 점을 찾아 이를 기준으로 VOP를 다시 분할하는 최적화된 영역 분할 방법을 제안하였다. 또한, 객체의 모양이 복잡한 경우에는 +90。 회전과 -90。 회전 병합을 추가함으로써 병합의 가능성을 높일 수 있다. 따라서 본 논문에서는 180。 회전 병합, +90。 회전 병합 그리고 -90。 회전 병합의 순서로 병합을 하는 다중 회전 병합을 제안하였다. 기존의 경계 블록 병합 기법에서는 병합이 되는 두 블록이 상관성이 적다면 큰 패딩의 효과를 기대할 수가 없게 된다. 따라서 본 논문에서는 병합을 해 준 후에 블록의 내용을 기반으로 패딩을 해 주었다. 제안한 방식은 기존의 경계 블록 병합 기법에 비해 동일 PSNR 하에서 약 5∼8(%) 정도 부호화 비트량을 감소시켰다.

• 한국지능시스템학회논문지
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• 제14권4호
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• pp.473-480
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• 2004

본 논문에서는 컬러 영상에서의 새로운 에지 검출 알고리즘을 제안한다. 제안된 적응 형태학적 WCNN알고리즘은 적응 형태학과 WCNN알고리즘으로 구성된다. 이는 입력된 컬러 영상의 임계값에 따라 적응 형태학을 이용하여 경계면의 차를 레벨업 시킨 후 WCNN 알고리즘을 이용하여 최적의 에지를 검출한다. 또한, 기존의 고정 마스크에지 검출방식을 탈피하여, 영상의 임계값의 차에 따라 가변적으로 변화하는 가변 BBM(Beak Y. H, Byun O. H, Moon S. R)마스크를 사용한다. 제안된 알고리즘의 기존의 연구에 비해 유용성을 검증하기 위해 본 논문은 30개의 컬러 영상의 모의 실험을 제공한다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2010년도 춘계학술대회 논문집
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• pp.123-125
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• 2010

As the exterior noise emitted by a vehicle is getting more and more attention, simulated pass-by measurements become more important. This well established method provides information about the total noise emitted by the vehicle. For a vehicle manufacturer it is not only interesting to know about the total noise but also to know how this total exterior noise is composed of different contributions, such as for example the contribution of the engine, the intake or exhaust system. Transfer path analysis (TPA) provides a separation of these contributions for each of the pass-by microphones alongside the track. Presented is a method for fast and efficient determination of the contributions of multiple sources using operational transfer path analysis (OTPA). The calculation of the transfer characteristics between the reference measurement points on the vehicle and the corresponding response points of both microphone lines are carried out while operation of the vehicle. As result of the contribution analysis from operational transfer path analysis, the characteristic noise level as function of the covered distance is displayed for all individual sound sources, thus providing in depth information for sound quality engineering.

• The Journal of Asian Finance, Economics and Business
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• 제8권2호
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• pp.231-241
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• 2021

The purpose of this study is to apply Structural equation modeling (SEM) analysis with Generalized Structured Component Analysis (GSCA) and translate the effect of Spiritual Marketing and Entrepreneurial Orientation on Sustainable Competitive Advantage with Marketing Capabilities as Mediation, especially for General BBM marketing at PT. Pertamina (Persero). The quantitative approach in this study uses a survey method by taking samples from the population. The survey was conducted by distributing questionnaires to respondents. Data analysis was performed using SEM and analyzed using the GSCA model. The population of this study consisted of 3,207 workers in central and regional marketing offices (Marketing Operation Region (MOR) spread throughout Indonesia. Therefore, a sample of 356 respondents was taken according to the Slovin formula. Spiritual marketing and entrepreneurial orientation directly influence the ability to improve Innovation which directly influences sustainable competitive advantage. Therefore, to develop a sustainable competitive advantage in marketing Pertamina's General BBM, it is necessary to implement spiritual marketing and improve entrepreneurial orientation. The novelty in this study lies in the unprecedented research on the role and position of spiritual marketing towards marketing capabilities and sustainable competitive advantage, combined with entrepreneurial orientation variables.

• Journal of Periodontal and Implant Science
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• 제26권2호
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• pp.491-510
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• 1996

This study was performed to evaluate the effects of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) on the characteristics of beagle dog's periodontal ligament (BPD) cells and bone marrow (BBM) cells which have the important role on the early stage of periodontal tissue regeneration in vitro. In control group, the cells ($1.5{\times}10^5$cells/ml) were cultured alone with Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum, $50{\mu]g/ml$ ascorbic acid, and 10mM/ml ${\beta}-glycerophosphate$. In experimental groups, growth factors, PDGF or EGF(10ng/ml), were added into the above culture condition. And then each group was characterized by examining the cell proliferation rate, amount of total protein synthesis, alkaline phosphatase activity at 1, 5, 9, 13, 17th day after seeding of cells into the culture wells. The results were as follows: 1. Both BPD and BBM cells in PDGF-treated group proliferated more rapidly than non-treated cells. This finding also was observed in EGF-treated group but it was not as prominent as that of PDGF-treated group. The proliferation rates of both cells showed the time-dependent pattern during experimental periods in all three groups. 2. Amount of total protein synthesis was more increased in PDGF-treated group than in control group. But no significant difference between EGF-treated group and control group was observed throughout experimental periods even though the tendency of amount of protein synthesis was time-dependent pattern. 3. Alkaline phosphatase activity also more increased in PDGF-treated group than control group. But slight decrease tendency was seen in both cells of EGF-treated group. From the above results, PDGF appeared to enhance the proliferation and cellular activities including amount of total protein synthesis and alkaline phosphatase activity of BPD and BBM cell, but EGF did not show notable effects. The optimal application of these growth factors was thought to be useful as the adjunctive means in periodontal regeneration procedures.

• Toxicological Research
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• 제8권1호
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• pp.95-107
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• 1992

Basolateral and brush border membrance (BLM and BBM) vesicles of renal proximal tubules were prepared from adult male New Zealand White rabbits to develop an experimental for assessment of nephrotoxicity. PAH uptakes using BLMV, and glucose and leucine uptakes using BBMV were measured in the rabbits treated uranyl acetate. In addition, urinalysis and histopathological studies were performed to investigate the correlationship with the membrance vesicle uptakes.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2012년도 춘계학술대회 논문집
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• pp.439-440
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• 2012

New analysis methods complement classical approaches in the vehicle NVH development by reducing and accelerating iteration steps to obtain a target sound. Therefore, tools are required that allow an integrative approach of sound engineering and structural analysis and enable a precise simulation and modification based on measured data. The Response Modification Analysis (RMA) is such a hybrid solution, which provides indications of relevant transfer paths taking into account the sensitivity of response channels to modifications of reference channels.