• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.21 no.1
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• pp.70-82
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• 2008

Objective : The purpose of this study is to examine the effects of Baickbujasan(BB) on the skin damage and depigmentation. Method : The inhibition of tyrosinase activity, melanogenesis and cell viability in cultured B16 melanoma cells were measured. In order to test effects of reduction of melanogenesis, B16 F-10 mouse melanoma stem line was employed to extract melanin from cultured cell, where BB was added or not, and was dissolved in alkali for colorimetric analysis. Also, in order to test skin alteration in C57BL/6 after UV irradiation, the animals were grouped into a UV urradiation group and UV irradiation after BB application group. Dopa oxidase tissue staining was excuted to invesitage the change in the distribution of active melanin cell. The distribution of active melanin cell in inner skin of iNOS after damage from UVB irradiation and the manifestation condition of P53 which takes part in natural death of keratinocyte were examined. Result : The results indicate that BB has significant effects on tyrosinase activity, and melanogenesis in vivo test. BB seems to reduce C57BL/6, external dermatological damage, for instance, erythematous papule, eczema, loss of keratinocyte, reduction in pus, and relieves dermatological damages. Conclusion : BB can be applied externally for UV protection and depigmentation.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.448-452
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• 2015

The bacterial strain that was isolated from chinese cabbage rhizosphere, showed inhibition of yeast growth. This strain was identified as Pseudomonas fluorescens BB2 by API 20NE test and 16S rRNA gene sequence analysis. P. fluorescens BB2 strain produced antibiotics against yeast as a secondary metabolite effectively when the culture was carried out in YM medium with 3% glucose at $20^{\circ}C$. The protein antibiotic of BB2 strain which was concentrated by ammonium sulfate precipitation and n-butanol extraction inhibited the growth of yeast with the minimal inhibitory concentration of $10{\mu}g/ml$ against Candida albicans KCTC 7965, and the growth of yeast was completely inhibited at $80{\mu}g/ml$. The hydrophilic fraction of n-butanol extraction inhibited the growth of Bacillus cereus ATCC 21366, showed orange halo on chrome azurol S plate, which means the fraction contained iron chelating siderophore. The results of crystal violet uptake through the cell membrane showed that membrane permeability was increased about 9% than control, when the concentration of hydrophobic antibiotic against yeast C. albicans was $60{\mu}g/ml$. As a result, the antibiotic produced by P. fluorescens BB2 against yeast Candida is considered antimicrobial peptide, and this is the first report in the genus Pseudomonas.

• Journal of the Society of Naval Architects of Korea
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• v.59 no.5
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• pp.288-295
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• 2022

In this study, the force and moment acting on a Joubert BB2 submarine model at depths near the free surface were measured through a captive model test with the scale ratio of 1/15. Based on the experiment, the pitch moment and heave force due to the "Tail suction effect", including the change in surge force with depth near the free surface, were quantitatively analyzed. The change of force and moment according to the relative position of the sail and the free surface was reviewed with the free surface waves generated for each depths. As a result, the angle of attack of the hull to counteract the pitch moment induced by the tail suction effect was derived. The effect of the hydrostatic moment component according to the angle of attack on the equilibrium of pitch moment was also taken into account. The control plane performance tests for the X-type rudder and sail plane were conducted in snorkel and surface depth conditions to figure out the control plane angles for the neutral level flight of the submarine at near free surface. The results of this study are expected to be used as a reference data for the neutral level flight of the submarine at near free surface operation in the free running model test as well as numerical studies.

• Communications for Statistical Applications and Methods
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• v.9 no.3
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• pp.607-618
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• 2002

Crossover design is often used in clinical trials about chronic diseases like hypertension, asthma and arthritis. In this paper, we suggest nonparametric approaches of Friedman-type rank test based on Bernard-van Elteren test and of aligned method keeping the information of blocks based on the AB/BA/AA/BB crossover design. The simulation results are presented to compare experimental error and power of several methods.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.545-548
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• 2001

Microbiologically influenced corrosion (MIC) of metal is common in the natural environment and sulfate reducing bacteria are representative microorganisms for MIC. We found that biofilm fomlation by SRB on the metal surface might be controlled by quorum sensing, which is a cell density dependent regulation of cell metabolism. As cell free culture fluids (spent media) of Desulfovibrio vulgaris and D. desulfuricans were tested for quontrn sensing related test strains, it was found that spent media of two SRB induced increased luminescence of Vibrio harveyi BB886 (sensor 1+, sensor 2-) and BB170 (sensor 1-, sensor 2+). Quorum activities of D. vulgaris and D. desulfuricans appeared to be parallel to growth patterns, i.e., it was low in the lag phase, highly increased in the exponential phase, and reached maximum in the stationary phase. Interestingly, however, luminescence of V. harveyi BB886 and BB170 induced by a unit cell mass of the SHB showed a maximal peak in the late lag phase. Hence, it was suspected that quorum sensing of these two SHB play unknown roles in shifting cells from dormant to growth stages.

• Korean Journal of Veterinary Service
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• v.31 no.1
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• pp.57-70
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• 2008

The purpose of this study was to investigate the protective effects of the modified live vaccines against canine Bordetella bronchiseptica (Bb) and canine parainfluenza virus (CPIV) in puppies by nasal administration. A total of 24 puppies were classified as 3 groups consisting of 8, and each one was divided into two subgroups; vaccinated (n=4) and unvaccinated (n=4). Group I, group II and group III were challenged with Bb, CPIV, and Bb+CPIV, respectively. In group I vaccinated puppies (n=4) were experimentally challenged with Bb 2 weeks after vaccination and unvaccinated puppies (n=4) were experimentally challenged with Bb alone. The same methods of the above were applied for group II and group III. We carried out several studies including serum tests, isolation, and histopathological examination. The vaccinated puppies showed higher antibody titers than unvaccinated puppies and the titer sustained during the experimental period. In the isolation test, recovery period was shorter in the vaccinated subgroup than in the other. In clinical signs, the unvaccinated puppies showed the typical signs of tracheobronchitis (coughing, nasal and occular discharge), but another subgroup showed delayed incidence and mild clinical signs. The typical gross lesions and histopathological findings were observed in the unvaccinated puppies. In immunohistochemical findings, the vaccinated puppies showed little intensive in reaction for CPIV antigen than the other. It could be concluded that intranasal vaccination of modified live Bb and CPIV vaccine to puppies is effective to prevent canine infectious tracheobronchitis.

• Proceedings of the Safety Management and Science Conference
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• 2009.04a
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• pp.423-427
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• 2009

This study proposes the generation mechanism of various design matrix using generalized linear model for design of experiment. Design generation method of GLM analysis, factorial design(FD) with center points, ANOVA design with lack-of-fit test, and response surface design are introduced. In central composite(CC) design, orthogonal blocking and fractional factorial design(FFD) are presented. We compare the design of Box-Benhken(BB) and face-centred central compsite design.

• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.799-821
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• 1996

The ultimate goal of periodontal treatment has been to facilitate regeneration of diseased periodontal tissues, destroyed by inflammatory periodontal disease. For regeneration of the periodontium to occur, all of component tissues must be restored to their original position and architecture. Growth factors which were known to promote the cellular processes, ie, proliferation, migration and matrix synthesis, have been in the spotlight of current periodontics. Platelet-derived growth factor(PDGF) stimulates collagen and non collagen protein synthesis, migration and proliferation of periodontal ligament cells. Insulin-like growth factor(IGF) has potentials to induce collagen and bone matrix synthesis so that it regulates normal bone remodeling. Application of the combination have been known to facilitate formation of bone and cementum, and to synergistically interact to promote coronal migration and proliferation of periodontal ligament cells. These two growth factors have been reported to exhibit positive effect in the periodontally diseased teeth or class m furcation defects. The aim of the present study was to test the hypothesis that PDGF-BB alone or the combination of PDGF-BB and IGF-I can predictably enhance regeneration of the periodontium in the dehiscence defect. Following the resection of premolars, roots were embedded. After 12 weeks of healing period, standardized experimental $4{\times}4mm$ dehiscence defects were created on the mid-facial of the premolar roots in each of 4 young adult dogs. In control group, only methylcellulose gel was inserted in the defects. In experimental group I and II, gel with $2{\mu}g$ of PDGF-BB or $2{\mu}g$ of PDGF-BB and $1{\mu}g$ of IGF-I was inserted in the defects, respectively. At 8 weeks postsurgery, the dogs were sacrificed. The results were observed histologically and analyzed histomorphometrically.The results of this study were as follws. 1. The new cementum formation was $1.26{\pm}0.69mm$ in the control group, $1.80{\pm}0.84mm$ in the experimental group I, $1.93{\pm}0.51mm$ in the experimental group II. The experimental group III, the experimental group I, the control group were in the order of cementum formation without statistically significant differences between control and all experimental groups. 2. The new bone formation was $1.00{\pm}0.53mm$ in the control group, $1.53{\pm}0.63mm$ in the experimental group I, $l.33{\pm}0.45mm$ in the experimental group II. The experimental group I, the experimental group II, the control group were in the order of bone formation without statistically significant differences between control and all experimental groups. 3. The root resorption was $1.12{\pm}0.64mm$ in the control group, $1.34{\pm}0.73mm$ in the experimental group I, $0.79{\pm}0.59mm$ in the experimental group II without statistically significant differences between control and all experimental groups. These results suggested that the use of PDGF-BB alone or PDGF-BB and IGF-I in the dehiscence defects might facilitate periodontal regeneration in some degree, but has not shown statistically significant results.

• Proceedings of the KSRS Conference
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• v.1
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• pp.64-66
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• 2006

DATS will connect with IMPS and LHGS to perform the reception of sensor data and the transmission of user's meteorological data, LRIT and HRIT. MODEM/BB will perform the de-commutation of received sensor data as MI and GOCI raw data according to VCID before sending them to MI and GOCI IMPS, respectively. Especially, MODEM/BB in SOC needs to be connected to six clients that consist of the primary and backup IMPS of MSC, KOSC and SOC. On the other hand, LRIT and HRIT delivered from LHGS are encoded as VITERBI and modulated by MODEM/BB. Considering sensor data transmitted from COMS, the assumed format and size of CADU are described in this paper. Finally, results related to the status of received LRIT and HRIT by frame synchronizer in user station are also described.

• Proceedings of the IEEK Conference
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• 2003.07a
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• pp.406-409
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• 2003

본 논문에서는 인터넷 QoS(Quality of Service)보장을 위하여 제안된 Diff-Serv(Differentiated Service)모델을 구현하고, QoS 를 보장하지 않는 BE(Best Effort)서비스와 우선 순위가 높은 패킷을 처리하여 QoS 를 보장하는 EF(Expedited Forwarding) 서비스의 차이를 통하여 Diff-Serv 망의 우수성을 입증하고자 한다. Diff-Serv 망은 BB(Bandwidth Broker)와 에지라우터 그리고 다수의 코어라우터로 구성된다. BB 로부터 라우터에 QoS Policy를 전송하기 위하여 COPS(Common Open Policy Service) 프로토콜을 구현하였으며, 사용자와 BB 사이의 SLAT/RAR(Service Level Agreement/Resource Allocation Request)를 처리하기 위하여 Admission Control 이라는 명명한 자체 프로토콜을 개발하였다. 구현된 Diff-Serv 망에서 QoS 보장 테스트를 위하여 19.2Mbps 대역폭이 요구되는 HDTV 스트리밍 서비스를 이용하여 인위적으로 Background Traffic 을 발생시켰을 때, BE 서비스만을 지원하는 망에서는 현저하게 서비스의 질이 떨어지는 반면에 Diff-Serv 망에서는 QoS가 보장됨을 알 수 있었다.