• Title/Summary/Keyword: BAT

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Isolation and Properties of Cytoplasmic α-Glycerol 3-Phosphate Dehydrogenase from the Pectoral Muscle of the Fruit Bat, Eidolon helvum

  • Agboola, Femi Kayode;Thomson, Alan;Afolayan, Adeyinka
    • BMB Reports
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    • v.36 no.2
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    • pp.159-166
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    • 2003
  • Cytoplasmic $\alpha$-glycerol-3-phosphate dehydrogenase from fruit-bat-breast muscle was purified by ion-exchange and affinity chromatography. The specific activity of the purified enzyme was approximately 120 units/mg of protein. The apparent molecular weight of the native enzyme, as determined by gel filtration on Sephadex G-100 was $59,500{\pm}650$ daltons; its subunit size was estimated to be $35,700{\pm}140$ by SDS-polyacrylamide gel electrophoresis. The true Michaelis-Menten constants for all substrates at pH 7.5 were $3.9{\pm}0.7\;mM$, $0.65{\pm}0.05\;mM$, $0.26{\pm}0.06\;mM$, and $0.005{\pm}0.0004\;mM$ for L-glycerol-3-phosphate, $NAD^+$, DHAP, and NADH, respectively. The true Michaelis-Menten constants at pH 10.0 were $2.30{\pm}0.21\;mM$ and $0.20{\pm}0.01\;mM$ for L-glycerol-3-phosphate and $NAD^+$, respectively. The turnover number, $k_{cat}$, of the forward reaction was $1.9{\pm}0.2{\times}10^4\;s^{-1}$. The treatment of the enzyme with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) under denaturing conditions indicated that there were a total of eight cysteine residues, while only two of these residues were reactive towards DTNB in the native enzyme. The overall results of the in vitro experiments suggest that $\alpha$-glycerol-3-phosphate dehydrogenase of the fruit bat preferentially catalyses the reduction of dihydroxyacetone phosphate to glycerol-3-phosphate.

Comparative Analysis of Best Available Techniques Reference Documents on the Fertilizer Manufacture between Korea and European Union (비료제조업의 국내와 EU 최적가용기법 기준서 비교·분석)

  • Seo, Kyungae;Kim, Gahee;Kim, Eunseok;Seok, Heejeong;Shin, Sujeong;Kim, Younglan;Kang, Philgoo
    • Journal of Environmental Science International
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    • v.29 no.3
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    • pp.307-318
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    • 2020
  • The integrated permit system is applied to class 1 and 2 atmospheric and water pollutant discharge facilities in 19 sectors. The fertilizer sector should receive a permit for a period of four years, from 2019 to 2023. The purpose of this study is to investigate the differences between the Korean Best Available Techniques (BAT) reference document (K-BREF) and the European Union BAT reference document (EU-BREF) in terms of the process and emission characteristics of fertilizer manufacture. K-BREF is written by focusing on products, whereas EU-BREF is produced on focusing on manufacture process. There are five types of BATs(best available techniques economically achievable) in Korea. These BATs selected mainly to recover and reuse raw meterials and save energy. The number of BATs and BAT-AELs(BAT associated emission level) in K-BREF is smaller than that in EU-BREF. We suggest that BATs and those environmental management parameters in Korea need to further reflect the emission characteristics in the fertilizer sector.

Understanding and Improvement of the K-BREF (Korea BAT reference documents) for the Corrugated Cardboard Manufacturing Industry (골판지원지 제조업 최적가용기법 기준서의 이해와 개선사항)

  • Seo, Kyungae;Kim, Eunseok;Kim, Gahee;Khan, Jongbeom;Hong, Sukyoung;Kang, Philgoo
    • Journal of Environmental Science International
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    • v.29 no.5
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    • pp.559-573
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    • 2020
  • The purpose of this study analyzed the overview of corrugated cardboasrd manufacturing industry and then provide direction for improvement. The BREF (BAT reference document) is an important reference for licensees and officer, including the best available techniques for the industry and achievable environmental performance, technical characteristics, and economic information. In the corrugated cardboard manufacturing process, wastewater pollutants are generated throughout the production process, and water is used in the dissociation and aging process. Atmospheric emissions are mostly generated by steam production from boilers and incinerators for the dry process. SO2, NOx, CO2, CO, HCl, dust, VOC, and odor were common. In the EU-BREF (European union BAT reference documents) BAT for wastewater have taken up a relatively large proportion. Items of water pollutants in wastewater were common in COD, BOD, N, P, SS, and however EU-BREF had different pollutants such as AOX and salt compared to K-BREF. In order to improve the quality of the K-BREF, it is necessary to devise basic data research method and data acqusitiom method. Consideration should be given to additional environmental management techniques that reflect the emissions characteristics of the corrugated cardboard manufacturing process. In addition, further research is needed to develop methodologies for selecting BATs considering environmental and economic feasibility.

A Study On The Drive Characteristic from Rotor Shape of 1kw Class Interior Permanent Magnet Synchronous Motor (1kw 급 매입형 영구자석 동기전동기(IPMSM)의 회전자 형상에 따른 구동특성 연구)

  • Jeong, Tae-Cheol;Kim, Won-Kyu;Kim, Youn-Hyun
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.690_691
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    • 2009
  • 본 연구는 1kw급 매입형 영구자석 동기전동기(IPMSM)의 회전자의 최적 형상 설계를 위하여 여러 가지 타입의 형상을 실험 및 시뮬레이션을 통해 공극자속밀도, 쇄교자속, 역기전력, 코깅토크, 정격점에서의 최대토크, 최대토크 전류 위상각의 특성을 비교하여 구동성능을 검토하였다. 본 연구를 통하여 비교한 모델은 1kw급 매입형 영구자석 동기전동기의 기초 특성 데이터로 유용하게 이용될 수 있을 것으로 판단된다.

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The Study on Cap Design for Developing Tourist Souvenir: Applying Traditional Bat Motifs (관광상품개발을 위한 cap 디자인 연구 -전통박쥐문양의 응용을 중심으로-)

  • 김혜경;정성일
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.8
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    • pp.1209-1219
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    • 2000
  • The importance of souvenirs are increasing immensely these days owing to the increase of foreign visitors to our country, which forces us to turn our eyes to this field. Upon these reflections, this study aims at aims at developing cap designs that satisfy the foreign tourists needs and let them recognize the outstanding beauty of Korean traditional patterns by applying traditional motifs. The Korean traditional patterns and their symbolic meanings were researched as well as the previous studies on the designing of goods for foreign visitors which had traditional patterns applied. Furthermore, the overall tendencies of the purchase of souvenirs and goods by foreign tourists in Korea as well as in foreign countries were investigated. Next, the present state of apparel goods and caps for sightseers in Korea was analyzed according to their shapes, colors, and patterns. Finally, by using CAD system, new cap designs were developed applying traditional bat motifs in order to capture the traditional images for a modern sense of beauty.

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Ca2+ Uptake of the Sarcoplasmic Reticulum-Microsome of Fine Structure Pectoralis Major Muscle in the rabbit and the Korean Greater Horseshoe Bat* (토끼와 한국관박쥐 대흉근의 근소포체 마이크로솜의 Calcium Uptake에 관한 연구)

  • 오영근;양원석
    • The Korean Journal of Zoology
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    • v.38 no.1
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    • pp.10-19
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    • 1995
  • 동면동물인 한국관박쥐(Korean greater horseshoe bat)와 비롱면 롱물인 토끼의 근소포체가 지니는 생리 및 근조직의 형태적 특징을 비교해 보고자 본 실험을 수행하여 다음과 같은 결론을 내렸다 Caffeine과 Mgcl2는 농도별로 처리하고, p연와 온도에 변화를 주는 등 각각의 생리조건하에서 한국관박쥐와 토끼 대흉근 근소포체 마이크로솜의 C32' 흡수정도가 다르게 나타났으며, Ca2'ATPase 활성 역시 상이함을 보였는데. 이로 미루어 보아 in vivo 상태에서 두 동물의 근소포체가 지니는 생리적 특성에 차이가 있을 것으로 추측된다. 형태학적 측면에서도 근심유의 수기와 미토콘드리아의 크기에 있어서 뚜렷한 차이를 발견할 수 있었는데. 이는 두 동물의 대흉근이 수행하는 운동의 종류와 관련이 있을 것으로 생각된다.

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Buffer Overflow Detection Using Machine Pattern of BBL (BBL단위 기계어 패턴을 이용한 버퍼 오버플로우 탐지법)

  • Han, Kwang-Hee;Joeng, Young-Gil;Jin, Young-Taek
    • Proceedings of the Korea Information Processing Society Conference
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    • 2018.10a
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    • pp.178-180
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    • 2018
  • 버퍼 오버플로우 취약점은 자체적으로 외부코드를 실행시키거나 다른 취약점과 연계해 공격될 수 있는 취약점으로 이를 탐지하기 위해 많은 연구들이 진행되었다. 기존의 버퍼 오버플로우 취약점 탐지기법에 대한 연구는 취약함수를 검색하거나 동적오염분석으로 얻은 비 신뢰 데이터의 흐름을 감시하여 취약함수로 전파되는 걸 탐지하는 방식 이었다. 하지만 이러한 기법은 다양한 한계를 가진다. 본 논문은 이러한 문제들을 해결하기 위해 버퍼 오버플로우가 발생하는 기계어 패턴을 BBL단위로 감시하고, 비 신뢰 데이터의 흐름을 추적하여 기존의 탐지 사각지대를 없애는 방법을 제안한다.

The BAT AGN Spectroscopic Survey: concept, status, and future perspectives

  • Oh, Kyuseok;Ueda, Yoshihiro;Koss, Michael;Ricci, Claudio;Schawinski, Kevin;Trakhtenbrot, Benny;Lamperti, Isabella
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.2
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    • pp.32.4-33
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    • 2018
  • We present the BAT AGN Spectroscopic Survey (BASS) which is an optical/NIR spectroscopic survey of the least biased sample of hard X-ray selected local AGN. For more than a thousand AGN that identified through Swift-BAT hard X-ray all-sky survey, we are conducting dedicated spectroscopic observing runs using world-class telescopes such as ESO-VLT, Magellan, and Palomar. The goal of the project is measuring black hole mass, investigating supermassive blackhole growth and its structure, and providing a baseline for future X-ray missions that will perform deeper observations of more distant AGN. In this presentation, we briefly introduce the concept of the project, past and the current status, and future work.

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Design and Evaluation Security Control Iconology for Big Data Processing (빅데이터 처리를 위한 보안관제 시각화 구현과 평가)

  • Yun, Seong Yeol;Kim, Jeong Ho;Jeon, Sang Jun
    • Proceedings of the Korea Information Processing Society Conference
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    • 2020.11a
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    • pp.420-423
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    • 2020
  • 본 연구에서는 민간기업들이 전체적인 보안관제 인프라를 구축 할 수 있도록 오픈소스 빅데이터 솔루션을 이용하여 보안관제 체계를 구축하는 방법을 기술한다. 특히, 보안관제 시스템을 구축할 때 비용·개발시간을 단축 할 수 있는 하나의 방법으로 무료 오픈소스 빅데이터 분석 솔루션 중 하나인 Elastic Stack을 활용하여 인프라를 구축했으며, 산업에 많이 도입되는 제품인 Splunk와 비교실험을 진행했다. Elastic Stack을 활용해 보안로그를 단계별로 수집-분석-시각화 하여 대시보드를 만들고 대용량 로그를 입력 후 검색속도를 측정하였다. 이를 통해 Elastic Stack이 Splunk를 대체 할 수 있는 빅데이터 분석 솔루션으로서의 가능성을 발견했다.