• Title/Summary/Keyword: BAT

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Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR

  • Lee, Yun-Tai;Yun, Bo-Kyoung;Yoon, Jeong-Joong
    • The Journal of Korean Society of Virology
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    • v.28 no.2
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    • pp.147-155
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    • 1998
  • Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

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Characteristics of Auditory Stereocilia in the Apical Turn of the Echolocating Bats by Scanning Electron Microscopy

  • Kim, Jinyong;Jung, Yongwook
    • Applied Microscopy
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    • v.44 no.1
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    • pp.8-14
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    • 2014
  • The auditory system of the Korean greater horseshoe bat (Rhinolophus ferrumequinum korai, RFK) is adapted to its own echolocation signal, which consist of constant frequency (CF) element and frequency modulated (FM) element. In contrast, the Japanese long-fingered bat (Miniopterus schreibersii fuliginosus, MSF) emits FM signals. In the present study, the characteristics of stereocilia in RFK (a CF/FM bat) and MSF (a FM bat) were studied in the apical turn of the cochlea where the lower frequencies are transduced. Stereocilia lengths and numbers were quantitatively measured in RFK by scanning electron microscopy and compared with those of MSF. Each inner hair cells (IHCs) of RFK possessed three rows of stereocilia, whereas MSF possessed five rows of stereocilia. Gradients in stereocilia lengths and numbers of stereocilia of the IHCs of RFK were found to be less pronounced and fewer, respectively, than those of MSF. Each outer hair cells (OHCs) possessed three rows of stereocilia in both species. OHCs stereocilia in RFK that distinguished it from MSF were a shorter length and a greater number of stereocilia. These features suggest that the apical cochleas of RFK are adapted for the processing of higher frequency echolocation calls rather than that of MSF.

Sexual Maturation and Reproduction Cycle of the Bat Star, Asterina pectinifera (Echinodermata: Asteroidae) (별불가사리, Asterina pectinifera의 성 성숙과 생식주기)

  • Cho, In-Young;Lee, Jung-Sik;Yoo, Myoung-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.39 no.1
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    • pp.27-34
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    • 2006
  • Sexual maturation and reproductive cycle of the bat star Asterina pectinifera were investigated by histological method. Specimens were collected monthly in Donghwari, Gosunggun, Korea from January 2004 to February 2005. The bat star was dioecious. The gonads are composed of a number of gametogenic follicles. The gonadosomatic index (GSI) of female and male were reached the maximum in July (5.72, 4.54) and the minimum in December (0.89) and January, February (0.51), and the gonad index (GI) of female and male were reached the maximum in July (3.53, 3.91) and the minimum in August (0.95) and October to December (1.0), respectively. The main spawning was from August to September. The reproductive cycle of the bat star could be divided into five stages: in the female, inactive (November-February), early active (January February), late active (March-June), ripe (July), spent and degenerative (August-November) and in the male, inactive (November-February), early active (January-March), late active (April-June), ripe (July), spent and degenerative (August-October), respectively.

Population genetic structure based on mitochondrial DNA analysis of Ikonnikov's whiskered bat (Myotis ikonnikovi-Chiroptera: Vespertilionidae) from Korea

  • Park, Soyeon;Noh, Pureum;Choi, Yu-Seong;Joo, Sungbae;Jeong, Gilsang;Kim, Sun-Sook
    • Journal of Ecology and Environment
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    • v.43 no.4
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    • pp.454-461
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    • 2019
  • Background: Ikonnikov's whiskered bat (Myotis ikonnikovi) is found throughout the Korean Peninsula, as well as in Kazakhstan, Russia, Mongolia, China, and Japan. It is small-sized and primarily inhabits old-growth forests. The decrease and fragmentation of habitats due to increased human activity may influence the genetic structure of bat populations. This study was designed to elucidate the population genetic structure of M. ikonnikovi using mitochondrial genes (cytochrome oxidase I and cytochrome b). Results: The results showed that M. ikonnikovi populations from Korea have high genetic diversity. Although genetic differentiation was not detected for the COI gene, strong genetic differentiation of the Cytb gene between Mt. Jeombong and Mt. Jiri populations was observed. Moreover, the results indicated that the gene flow of the maternal lineage may be limited. Conclusions: This study is the first to identify the genetic population structure of M. ikonnikovi. We suggest that conservation of local populations is important for sustaining the genetic diversity of the bat, and comprehensive studies on factors causing habitat fragmentation are required.

The CRL Download Protocol for Vehicle in WAVE System (WAVE 시스템을 위한 차량용 CRL 다운로드 프로토콜)

  • Sun, Seol-Hee;Yoo, Kwon-Jeong;Choi, Beom-Jin;Kim, Eun-Gi
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.409-411
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    • 2014
  • WAVE 기술은 차량이 고속 이동환경에서 차량간 또는 차량과 인프라간 패킷을 짧은 시간 내에 주고 받을 수 있는 무선통신 기술이다. 본 논문의 목적은 차량이 WAVE 시스템에 통신 할 때 상대방의 인증서가 폐기 되었는지 확인하기 위한 CRL(Certificate Revocation List) 다운로드 프로토콜을 설계하는 것이다. CRL 다운로드 프로토콜은 WAVE 시스템 환경에 맞추기 위해 TCP(Transmission Control Protocol)가 아닌 UDP(User Datagram Protocol) 상에서 동작한다. 그리고 보안기능을 지원하기 위해 ECDSA 를 사용하여 상호 인증을 하고 ECIES 를 사용하여 인증서의 기밀성을 보장한다. 또한 이 프로토콜은 MAC 을 CRL 데이터에 붙여 데이터의 무결성을 보장하고, UDP 상에서 동작할 때 발생할 수 있는 데이터의 손실을 줄이기 위해 에러 및 흐름제어 방식으로 Selective repeat ARQ 를 사용한다.

Characterization of bat coronaviruses: a latent global threat

  • Fang, Manxin;Hu, Wei;Liu, Ben
    • Journal of Veterinary Science
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    • v.22 no.5
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    • pp.72.1-72.15
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    • 2021
  • It has been speculated that bats serve as reservoirs of a huge variety of emerging coronaviruses (CoVs) that have been responsible for severe havoc in human health systems as well as negatively affecting human economic and social systems. A prime example is the currently active severe acute respiratory syndrome (SARS)-CoV2, which presumably originated from bats, demonstrating that the risk of a new outbreak of bat coronavirus is always latent. Therefore, an in-depth investigation to better comprehend bat CoVs has become an important issue within the international community, a group that aims to attenuate the consequences of future outbreaks. In this review, we present a concise introduction to CoVs found in bats and discuss their distribution in Southeast Asia. We also discuss the unique adaptation features in bats that confer the ability to be a potential coronavirus reservoir. In addition, we review the bat coronavirus-linked diseases that have emerged in the last two decades. Finally, we propose key factors helpful in the prediction of a novel coronavirus outbreak and present the most recent methods used to forecast an evolving outbreak.

Regional differences in winter activity of hibernating greater horseshoe bats (Rhinolophus ferrumequinum) from Korea

  • Kim, Sun-Sook;Choi, Yu-Seong;Yoo, Jeong-Chil
    • Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.1-8
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    • 2019
  • Background: Hibernating bats exhibit ubiquitous winter activity in temperate zones, but there is considerable between- and within-species variety in their intensity and purpose. Bats may fly during winter for sustenance or travel to other hibernacula. This study compared inter-regional variation in the winter activity of the greater horseshoe bat (Rhinolophus ferrumequinum). We predicted that weather and hibernacula-environmental conditions would influence winter activity patterns. Results: Winter activity patterns differed between regions. In the Anseong area, we confirmed movement inside the hibernaculum, but in Hampyeong, we observed movement both inside and between hibernacula. The two regions differ by $4^{\circ}C$ in average winter temperatures. Anseong experiences 22 days during which average daily temperatures exceeded $5^{\circ}C$, whereas Hampyeong experienced 50 such days. During the hibernating period, bat body weight decreased by approximately 17-20% in both regions. Conclusions: Ambient temperatures and winter-roost environments appear to be behind regional differences in hibernating bat activity. As winter temperatures in Korea do not favor insect activity, feeding probability is low for bats. However, bats may need to access water. At Anseong, underground water flows inside the hibernaculum when the reservoir outside is frozen. At Hampyeong, the hibernaculum does not contain a water source, but the reservoir outside does not freeze during winter. In conclusion, water-source location is the most likely explanation for regional variation in the winter activity of hibernating bats.

Histochemical Analysis of the Cone cells in the Retina of the Greater Horseshoe Bat Rhinolophus ferrumequinum (한국관박쥐 망막 원뿔세포의 조직화학적 분석)

  • Jeon, Young-Ki;Joo, Young-Rak;Ye, Eun-Ah;Kim, Moon-Sook;Jeon, Chang-Jin
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.2
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    • pp.187-191
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    • 2013
  • Purpose: This study was done to understand the visual system of bat by analyzing the distribution of middle/long (ML) opsin cone photoreceptors in the retina of the greater horseshoe bat. Methods: Experiments have been performed by standard immunocytochemical techniques on retina of the greater horseshoe bat Rhinolophus ferrumequinum. Results: The estimated numbers of ML cones were $27,336{\pm}2,130$ cells and the mean density of them was $7,854{\pm}268cells/mm^2$ among the four retinas. S opsin was appeared a little immunoreactivity in the outer segments of outer nuclear layer of cones. Conclusions: From the well organized spatial distributions of ML opsin and the immunoreactivity of S opsin in the retinas, the greater horseshoe bats have the functions not only reacting in the photopic vision but being able to distinguish the colors.

Effects of High Fat Diet on Serum Leptin and Insulin Level and Brown Adipose Tissue UCP 1 Expression in Rats (흰쥐에서 고지방식이가 혈중 렙틴 및 인슐린과 갈색지방조직의 UCP 1 발현에 미치는 영향)

  • 홍경희;강순아;김소혜;조여원
    • Journal of Nutrition and Health
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    • v.34 no.8
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    • pp.865-871
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    • 2001
  • The adipose tissue hormone leptin has been proposed to be involved in the regulation of flood intake and energy expenditure via thermogenesis by uncoupling protein(UCP) in brown adipose tissue(BAT). The objective of the study was to examine the effects of high fat diet on the serum leptin levels, BAT UCPl expression and the body fat mass in rats after weaning. During experimental period of 12 weeks, 4 male Sprague-Dawley rats were killed for the baseline experiment at 4 weeks of age while the remaining rats were fed the two different diets: the control diet AIN-76A(n = 20), high fat(beef tallow) diet(n = 20) ad libitum, which provided 11.7% or 40% of calories as fat, respectively. At 16 weeks of age, the increase in the food efficiency ratio(FER) was related to fat mass in rats on high fat diet. Serum leptin level was increased by age and dietary high fat. There was no difference in serum insulin level between groups until 10 weeks of age, but rats fed high fat diet for 12 weeks showed hyperinsulinemia. The amount of body fat pads was increased significantly in high fat group compared to normal diet group. Visceral fat mass affected acutely by high fat diet, as a result, it was higher in rats fed high fat diet for 2 weeks than normal diet. At 16 weeks of age, BAT and visceral fat mass were significantly high in high fat group. Also, the serum leptin levels reflected the amount of body fat mass. BAT UCPI mRNA expression increased with age and dietary high fat. This study demonstrates that dietary high fat increased serum leptin levels, BAT UCPI expression and body fat mass. Futhermore, in rats fed high fat diets, the increases in leptin and UCPI expression counteracts only in part the excess adiposity and obesity.

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A Dynamic Offset and Delay Differential Assembly Method for OBS Network

  • Sui Zhicheng;Xiao Shilin;Zeng Qingji
    • Journal of Communications and Networks
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    • v.8 no.2
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    • pp.234-240
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    • 2006
  • We study the dynamic burst assembly based on traffic prediction and offset and delay differentiation in optical burst switching network. To improve existing burst assembly mechanism and build an adaptive flexible optical burst switching network, an approach called quality of service (QoS) based adaptive dynamic assembly (QADA) is proposed in this paper. QADA method takes into account current arrival traffic in prediction time adequately and performs adaptive dynamic assembly in limited burst assembly time (BAT) range. By the simulation of burst length error, the QADA method is proved better than the existing method and can achieve the small enough predictive error for real scenarios. Then the different dynamic ranges of BAT for four traffic classes are introduced to make delay differentiation. According to the limitation of BAT range, the burst assembly is classified into one-dimension limit and two-dimension limit. We draw a comparison between one-dimension and two-dimension limit with different prediction time under QoS based offset time and find that the one-dimensional approach offers better network performance, while the two-dimensional approach provides strict inter-class differentiation. Furthermore, the final simulation results in our network condition show that QADA can execute adaptive flexible burst assembly with dynamic BAT and achieve a latency reduction, delay fairness, and offset time QoS guarantee for different traffic classes.