• Molecules and Cells
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• v.25 no.2
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• pp.231-241
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• 2008

Indole glucosinolates (IG) play important roles in plant defense, plant-insect interactions, and stress responses in plants. In an attempt to metabolically engineer the IG pathway flux in Chinese cabbage, three important Arabidopsis cDNAs, CYP79B2, CYP79B3, and CYP83B1, were introduced into Chinese cabbage by Agrobacterium-mediated transformation. Overexpression of CYP79B3 or CYP83B1 did not affect IG accumulation levels, and overexpression of CYP79B2 or CYP79B3 prevented the transformed callus from being regenerated, displaying the phenotype of indole-3-acetic acid (IAA) overproduction. However, when CYP83B1 was overexpressed together with CYP79B2 and/or CYP79B3, the transformed calli were regenerated into whole plants that accumulated higher levels of glucobrassicin, 4-hydroxy glucobrassicin, and 4-methoxy glucobrassicin than wild-type controls. This result suggests that the flux in Chinese cabbage is predominantly channeled into IAA biosynthesis so that coordinate expression of the two consecutive enzymes is needed to divert the flux into IG biosynthesis. With regard to IG accumulation, overexpression of all three cDNAs was no better than overexpression of the two cDNAs. The content of neoglucobrassicin remained unchanged in all transgenic plants. Although glucobrassicin was most directly affected by overexpression of the transgenes, elevated levels of the parent IG, glucobrassicin, were not always accompanied by increases in 4-hydroxy and 4-methoxy glucobrassicin. However, one transgenic line producing about 8-fold increased glucobrassicin also accumulated at least 2.5 fold more 4-hydroxy and 4-methoxy glucobrassicin. This implies that a large glucobrassicin pool exceeding some threshold level drives the flux into the side chain modification pathway. Aliphatic glucosinolate content was not affected in any of the transgenic plants.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5371-5376
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• 2015

Berberine (B1), isolated from stems of Coscinium fenestratum (Goetgh.) Colebr, was used as a principle structure to synthesize three phenolic derivatives: berberrubine (B2) with a single phenolic group, berberrubine chloride (B3) as a chloride counter ion derivative, and 2,3,9,10-tetra-hydroxyberberine chloride (B4) with four phenolic groups, to investigate their direct and indirect antioxidant activities. For DPPH assay, compounds B4, B3, and B2 showed good direct antioxidant activity ($IC_{50}$ values=$10.7{\pm}1.76$, $55.2{\pm}2.24$, and $87.4{\pm}6.65{\mu}M$, respectively) whereas the $IC_{50}$ value of berberine was higher than $500{\mu}M$. Moreover, compound B4 exhibited a better DPPH scavenging activity than BHT as a standard antioxidant ($IC_{50}=72.7{\pm}7.22{\mu}M$) due to the ortho position of hydroxyl groups and its capacity to undergo intramolecular hydrogen bonding. For cytotoxicity assay against human fibrosarcoma cells (HT1080) using MTT reagent, the sequence of $IC_{50}$ value at 7-day treatment stated that B1 < B4 < B2 ($0.44{\pm}0.03$, $2.88{\pm}0.23$, and $6.05{\pm}0.64{\mu}M$, respectively). Berberine derivatives, B2 and B4, showed approximately the same level of CAT expression and significant up-regulation of SOD expression in a dose-dependent manner compared to berberine treatment for 7-day exposure using reverse transcription-polymerase chain reaction (RT-PCR) assays. Our findings show a better direct-antioxidant activity of the derivatives containing phenolic groups than berberine in a cell-free system. For cell-based system, berberine was able to exert better cytotoxic activity than its derivatives. Berberine derivatives containing a single and four phenolic groups showed improved up-regulation of SOD gene expression. Cytotoxic action might not be the main effect of berberine derivatives. Other pharmacological targets of these derivatives should be further investigated to confirm the medical benefit of phenolic groups introduced into the berberine molecule.

• Journal of the Korean Ceramic Society
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• v.42 no.1
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• pp.22-27
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• 2005

The preparation of B4C by SHS in $B_{2}O_{3}-Mg-C$ system was investigated in this study. In the preparation of B4C, the effect on reactivity and reaction products of the initial pressure of inert gas in reactor, the content of Mg and C in mixture was investigated. The minimum initial pressure of inert gas in reactor for SHS reaction in this system was 25 atm, and as the pressure increased, the concentration of unreacted Mg decreased and combustion temperature increased. At the initial inert gas pressure in reactor of 25 atm, the optimum composition for the preparation of pure B4C was $2B_{2}O_{3}+6.3Mg +0.94C$. The B4C synthesized in this condition had an irregular shape and the particle size of $1\~3{\mu}m$.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.1
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• pp.106-113
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• 2003

In this paper, we proposed low noise amplifier for 2.4GHz ISM frequency with CMOS technology. The property of noise and gain is improved by cascode architecture. The architecture, which common source output of cascode is connected to input of parallel MOS, reduce IM. The LNA results based on Hynix 0.35${\mu}{\textrm}{m}$ 2poly 4metal CMOS processor with a 3.3V supply. It achieves a gain of 13dB, noise figure of 1.7dB, IP3 of 8dBm, Input/output matching of -31dB/-28dB, reverse isolation of -25dB. and power dissipation of 4.7mW with HSPICE simulation. The size of layout is smaller than 2 ${\times}$ 2mm with Mentor.

• Journal of the Korean earth science society
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• v.31 no.4
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• pp.313-321
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• 2010

The landform of the study area is defined as a small-scale alluvial plain in the valley bottom adjacent to the coast. By the aggradation of alluvial materials, this valley bottom plain was formed in dissected parts of low hills. For the purpose of reconstructing the palaeo climate environment in this study, $\delta^{13}C$ analysis and soil organic carbon analysis are therefore employed. Main results of this study are as follows: Section I: the period of $6,600{\pm}60yr$ B.P.$-5,350{\pm}60yr$B.P. was mostly in warm and humid climate environment. A little changes of the humid environment are detected as subdry (or sub-humid)${\rightarrow}$humid in terms of the dryness and wetness. Section II: the period of $5,350{\pm}60yr$ B.P.-2,200 yr B.P. was in warm and humid climate environment, which is similar to the present. However, The sediments between $4,720{\pm}60yr$ B.P. and $4,210{\pm}50 yr$ B.P. experienced the most humid climate environment of all studied sedimentary layers. After $4,210{\pm}50 yr$ B.P., the environment started to change from the humid to the sub-humid (or sub-dry) climate. Section III: the period of 2,200 yr B.P.$-210{\pm}60 yr$ B.P. was distinguish from previous two sections as the environmental changes to sub-humid (or sub-dry) climate was apparent.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.5
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• pp.383-389
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• 2010

Co-B, Co-P-B, Co-Ni-B and Co-Ni-P-B catalysts supported on Ni foam were prepared using electroless plating in the present study. The surface morphology of the catalysts/Ni foam was observed using SEM and EDS analysis. The Co-Ni-P-B/Ni foam catalyst showed the superior performance on hydrogen generation due to the uniform formation of catalyst particles on the Ni foam surface. The characteristics of hydrogen generation with Co-Ni-P-B/Ni foam catalyst was investigated at the variety of $NaBH_4$ and NaOH concentrations. Durability test was performed, resulting in the stable hydrogen generation for 6 hours.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.187-193
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• 2015

Candida (also known as Pseudozyma) antarctica lipase B (CAL-B) has been intensely studied in academic and industrial fields. However, the research related to its homologous enzymes has been rarely reported. In the current investigation, protein sequence similarity search of CAL-B has been conducted and six homologous protein sequences were identified. After the syntheses of their codon-optimized genes, the synthetic genes have been cloned into a periplasmic expression vector to express in Escherichia coli. Among six homologous sequences, four sequences were successfully expressed in E. coli. The hydrolytic activities of the expressed proteins towards 4-nitrophenyl acetate and 4-nitrophenyl butyrate were measured and compared with those of CAL-B to identify whether the expressed proteins work as a hydrolase. It has been revealed that the expressed proteins can hydrolyze the substrates and the specific activities were determined as $(1.3-30){\times}10^{-2}{\mu}mol/min/mg$, which are lower than those of CAL-B. Among these homologous enzymes, Pseudozyma hubeiensis SY62 exhibits the comparable enantioselectivity to that of CAL-B towards the hydrolysis of (${\pm}$)-1-phenylethyl acetate.

• Journal of radiological science and technology
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• v.28 no.1
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• pp.1-7
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• 2005

High-quality single crystals of pure $Li_2B_4O_7$ as well as $Li_2B_4O_7$ doped with Cu, Mn and Mg impurities (1.0mol%, respectively) have been grown from the melt of $Li_2CO_3+2B_2O_3$ by Czochralski method in platinum crucibles. To study the thermoluminescent properties, $Li_2B_4O_7$ series single crystal TLDs were made by cutting in the size of $4{\times}5{\times}1\;mm^3$. The glow curves show two or three peaks which can be easily deconvoluted. It is observed that room temperature($20{\sim}30^{\circ}C$) fadings of the dosimetric peaks of $Li_2B_4O_7$ series single crystal TLDs were about 10 % for 30 days. The relative photon energy response for $Li_2B_4O_7$ series single crystal TLDs were about 85 % when the responses were normalized to that measured with $^{60}Co\;(1.25\;MeV)\;{\gamma}-rays$. The measured data are in a good agreement with theoretical ones. The $Li_2B_4O_7$ series single crystal TLDs fabricated in this work can be used for monitoring personal and environmental radioactivity.

• The KIPS Transactions:PartB
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• v.18B no.5
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• pp.249-254
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• 2011

FGS (fine granularity scalability) supporting scalability in MPEG-4 Part 2 is a scalable video coding scheme that provides bit-rate adaptation to varying network bandwidth thereby achieving of its optimal video quality. In this paper, we proposed FGS coding scheme which performs one more bit-plane coding for residue signal occured in the enhancement-layer of the basic FGS coding scheme. The experiment evaluated in terms of video quality scalability of the proposed FGS coding scheme by comparing with FGS coding scheme of the MPEG-4 verification model (VM-FGS). The comparison was conducted by analysis of PSNR values of three tested video sequences. The results showed that when using rate control algorithm VM5+, the proposed FGS coding scheme obtained Y, U, V PSNR of 0.4 dB, 9.4 dB, 9 dB averagely higher and when using fixed QP value 17, obtained Y, U, V PSNR of 4.61 dB, 20.21 dB, 16.56 dB averagely higher than the existing VM-FGS. From results, we found that the proposed FGS coding scheme has higher video quality scalability to be able to achieve video quality from minimum to maximum than VM-FGS.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.386-389
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• 2003

본 논문에서는 Bluetooth applications를 위한 Bipolar Transistor low noise amplifier (LNA)를 설계하였다. 설계된 LNA는 2.4GHz에서 14.31[dB]의 Gain과 1.59[dB]의 NF를 보였다. 또한 S11은 -13.5[dB], S22는 -21.4[dB]의 양호한 값은 보였다.