• 한국미생물·생명공학회지
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• 제52권1호
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• pp.1-14
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• 2024

본 연구에서는 제주도 양식장에서 사육되고 있는 붉바리 장내에서 균주를 분리·동정하여, 산업적으로 활용할 수 있는 프로바이오틱스의 잠재적 가능성을 평가하였다. 붉바리 장내에서 순수 분리된 129균주를 대상으로 1차적으로 자체적인 간이 항균활성 실험을 통해 clear zone이 10 mm 이상 되는 12균주를 선별하여 내담즙성, pH 농도별 내산성, 항산화 활성, 항생제 내성, API kti을 이용한 생리생화학적 특성 실험에 이용하였다. 각 특성 실험을 통해 probiotics으로 잠재적 가능성을 가진 균으로 선발되어진 미생물은 G1, G3, G15, G21, B1, B2, B3, B5 총 8종이다. 8종 중 B균주 그룹(B1, B2, B3, B5)은 G균주 그룹(G1, G3, G15, G21)보다 유의하게 높은 활성을 나타내었다. 선발되어진 미생물의 16S rRNA 염기서열 분석을 통한 계통 분석을 한 결과를 바탕으로 B1 균주는 L. paracasei B1, B2는 L. lactis B2, B3 균주는 Lb. plantarum B3, B5 균주는 L. lactis subsp. hordniae B5, G1은 B. licheniformis G1, G3는 B. velezensis G3, G15는 B. frigoritolerans G15, G21 균주는 B. pumilus G21로 명명하였다.

• 한국식품저장유통학회지
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• 제30권1호
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• pp.98-108
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• 2023

본 연구에서는 HPLC를 이용하여 국내 식품자원 135종의 비타민 B₁과 B₃ 함량을 분석한 결과, 비타민 B₁은 0.019-28.218 mg/100 g, 비타민 B₃는 0.077-32.412 mg/100 g 범위로 검출되었다. 식물성 식품자원 42종 중 비타민 B₁은 11종, 0.071-0.884 mg/100 g 범위로, 비타민 B₃는 31종, 0.145-13.883 mg/100 g 범위에서 검출되었다. 동물성 식품자원 36종 중 비타민 B₁은 27종, 0.031-10.055 mg/100 g 범위로, 비타민 B₃는 34종, 0.077-32.412 mg/100 g 범위에서 검출되었다. 가공식품은 57종 중 비타민 B₁은 18종, 0.019-28.218 mg/100 g 범위로, 비타민 B₃는 43종, 0.089-7.072 mg/100 g 범위에서 검출되었다. 식품자원 중 비타민 B₁은 가공식품인 라면스프에서 28.218 mg/100 g, 비타민 B₃는 소간에서 32.412 mg/100 g으로 가장 많이 함유하고 있었다. 본 연구에서 제시된 수용성 비타민인 비타민 B₁과 B₃ 함량 정보는 국가표준식품성분표에 기초자료로 제공되어 국민 영양 증진에 기여하고자 한다.

• 한국식물병리학회지
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• 제11권4호
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• pp.292-297
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• 1995

We developed an in vitro assay method for evaluating plant growth promotion and providing an evidence that the growth promotion is rendered by growth enhancing factors. The amendment of culture filtrates of Trichoderma harzianum T95 and Gliocladium virens G872 and G872B in Murashige and Skoog (MS) agar medium enhanced the cotyledon growth of cucumber in terms of fresh weight and primary leaf area of cucumber cotyledon cuttings, of which the treatment of G. virens G872B was the most effective. The mycelial culture filtrate of G872B was more effective in the growth promotion than its conidial germling filtrate. The addition of 1% sucrose in MS mineral medium with 0.1% culture filtrates of the antagonists (T95 and G872B) was optimum for enhancing the effect of the filtrates on the growth of cotyledon cuttings in vitro. When cucumber seeds treated with G872B, Pseudomonas putida Pf3 or the G872B-Pf3 mixture were planted in a greenhouse, the rate of seed germination, biomass of shoot and root, and yield of cucumber fruits were increased, especially by G872B or the G872B-Pf3 mixture. Correspondingly, cucumber fruit yields in early to middle-cycles of harvest were significantly greater in the plots of G872B than the control and Pf3-treated plots, and the final yield was highest in the plots of the G872B-Pf3 mixture applications.

• 한국산학기술학회논문지
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• 제12권3호
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• pp.1373-1379
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• 2011

B3G 네트워크에서 서비스 제공자와 사용자 사이에 SLA (Service Level Agreement)가 정해지면 서비스 제공자는 정해진 QoS 맞게 서비스를 제공하여야 한다. 하지만 네트워크는 상황은 시간에 따라 변하며, 서비스 제공 환경이 변할 수 있다. 따라서 네트워크 서비스에 대해서 동적인 QoS 관리 방안이 고려되어야 한다. 본 논문에서는 B3G 융합망에서 네트워크 상황에 따라 동적인 QoS를 위한 핸드오버 방안에 대해 제안한다. B3G 네트워크에서 동적인 QoS 관리를 위하여 이종의 망을 이용하게 된다.

• Bulletin of the Korean Chemical Society
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• 제24권6호
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• pp.792-796
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• 2003

Polychlorinated dibenzo-p-dioxins (PCDDs) are extremely toxic and persistent environmental pollutants. Their chemical reactivities and other physicochemical/biological properties show a strong dependence on the chlorination pattern. With increasing the number of chlorines, dioxin congeners become more electronegative and gain higher electron affinities. The vertical electron affinities (VEA) are related with the LUMO energies of neutral molecules. LUMO energies of all PCDD congeners were calculated at the B3LYP/6-31G** level and those of some selected congeners at the level of B3LYP/6-311G**//B3LYP/6-31G** and B3LYP/cc-pvtz/ /B3LYP/6-31G**. The total energies of neutral and anionic species for dibenzo-p-dioxins (DD), 1469-TCDD, 2378-TCDD, and OCDD were calculated at the level of B3LYP/6-31G**, B3LYP/aug-cc-pvdz, and B3LYP/ aug-cc-pvtz//B3LYP/6-31G**. By using the four congeners with D2h symmetry as reference molecules, we could estimate VEA (B3LYP/aug-cc-pvdz) of 75 PCDD congeners based on the linear correlations between LUMO energy and VEA (B3LYP/6-31G**) and between VEA (B3LYP/6-31G**) and VEA (B3LYP/aug-ccpvtz// B3LYP/6-31G**). Results show that all PCDDs with the number of Cl ≥ 3 have positive electron affinities. The PCDD electron affinity values provided in this work can be a useful data set in understanding the congener-specific reactivities of dioxins in various environmental media.

• 한국식품영양학회지
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• 제30권4호
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• pp.710-718
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• 2017

Contents of water soluble vitamin $B_6$ in a total of 62 agricultural products cultivated in local areas in Korea were analyzed by high performance liquid chromatography with fluorescence detector (HPLC/FLD). To verify the method of vitamin $B_6$ analysis, a quality control chart was formulated with in-house control using a mixture of broccoli and shiitake mushrooms. Among cereals, high content of vitamin $B_6$ measured $234.3{\sim}260.3{\mu}g/100g$ in dried mung bean and soybean. Vitamin $B_6$ content of non-glutinous and glutinous black rice measured $105.0{\mu}g/100g$ and $129.7{\mu}g/100g$, respectively. In vegetables, high content of vitamin $B_6$ were measured in passion fruit ($104.3{\mu}g/100g$), gat ($55.7{\sim}84.3{\mu}g/100g$), gomchwi ($31.3{\sim}88.0{\mu}g/100g$) and garlic ($72.7{\sim}98.3{\mu}g/100g$). Among fruits, gold kiwi 'Zespri' and green kiwi 'Hayward' revealed high vitamin $B_6$ content of $116.3{\mu}g/100g$ and $78.7{\mu}g/100g$, respectively. In persimmons, daebongsi had high vitamin $B_6$ content ($36.0{\sim}72.7{\mu}g/100g$) than bansi and sweet persimmon. Vitamin $B_6$ content in dried jujube and persimmon increased more than $86.7{\mu}g/100g$ compared to fresh materials. Among specialty crops, green tea powder ($64.7{\sim}251.0{\mu}g/100g$) and sansuyu ($172.3{\mu}g/100g$) revealed high content. Of mushrooms, vitamin $B_6$ content of Sparassis crispa ($139.3{\mu}g/100g$) was the highest. Vitamin $B_6$ content information of agricultural products in local areas in Korea collected from this experiment will be used as valuable preliminary data for grasp national nutritional status.

• 치위생과학회지
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• 제22권3호
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• pp.156-163
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• 2022

Background: This study attempted to apply resin infiltrant (RI) as a method to maintain the effect of tooth bleaching treatment and compared it with fluoride varnish (FV) or artificial saliva to evaluate the effect. Methods: Sixty healthy lozenge specimens were classified into five groups. Group 1 was the negative control group, and discoloration was induced after artificial saliva treatment of the tooth specimen (G1_S+C). Group 2 was a positive control group, in which pigmentation was induced after bleaching treatment and artificial saliva treatment (G2 _B+S+C). Coloration was induced in group 3 (experimental group 1) after bleaching treatment and artificial saliva treatment, followed by application of fluorine varnish (G3_B+FV+S+C). Coloration was induced in Group 4 (experimental group 2) after applying RI after bleaching treatment and artificial saliva treatment (G4_B+RI+S+C). Pigmentation was induced in group 5 (experimental group 3) after bleaching treatment and artificial saliva treatment, followed by acid treatment (etching) and treatment with RI (G5_B+E+RI+S+C). Coffee and wine were used to induce discoloration. The lightness value (L^*) of the CIE L^*a^*b^* color system was obtained by image analysis. Kruskal-Wallis H analysis was performed for the mean difference in L^* values by group. Results: When coloration was induced with coffee, there was no significant difference in L^* value between artificial saliva (G2 _B+S+C), FV (G3_B+FV+S+C), and RI (G4_B+RI+S+C, G5_B+E+RI+S+C) groups. There was no significant difference in L^* values between the artificial saliva (G2 _B+S+C), FV (G3_B+FV+S+C), and RI (G4_B+RI+S+C, G5_B+E+RI+S+C) groups, even in the case of wine induced coloration. Conclusion: It was confirmed that artificial saliva or RI treatment had similar effects to the FV previously used to maintain the effect of tooth bleaching treatment.

• 한국식품영양과학회지
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• 제46권8호
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• pp.944-951
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• 2017

국내 다소비 명절 및 제사음식 31종에 함유된 비타민 $B_1$, $B_2$ 및 $B_3$ 함량을 형광검출기(HPLC/FLD) 및 자외부 흡광검출기(HPLC/DAD)를 사용하여 분석하였다. 주기적으로 내부 분석품질 관리를 실시하여 분석 결과가 신뢰성 있는 데이터임을 확인하였고, 국제 정도 관리에 참여하여 정밀한 분석능력을 검증받았다. 명절 및 제사음식 31종에 함유된 비타민 $B_1$, $B_2$ 및 $B_3$ 함량을 정량적으로 평가한 결과 비타민 $B_1$의 경우 나물류 중 가지나물(0.130 mg/100 g), 전류 중 육원전(0.973 mg/100 g), 찜류 중 병어찜(0.082 mg/100 g), 참꼬막(0.079 mg/100 g), 낙지숙회(0.079 mg/100 g)가 높은 함량을 나타내었다. 비타민 $B_2$의 경우에는 나물류 중 시금치나물(0.140 mg/100 g), 전류 중 동태전(0.264 mg/100 g), 찜류 중 도미찜(0.256 mg/100 g), 전어찜(0.246 mg/100 g)이 가장 높았다. 한편 비타민 $B_3$는 나물류 중 무나물(0.245 mg/100 g), 전류 중 육원전(0.1.223 mg/100 g), 찜류 중 전어찜(0.982 mg/100 g)이 가장 높은 함량을 나타내는 것으로 조사되었다. 수용성 비타민 중 비타민 $B_1$의 경우 비타민 $B_2$ 및 $B_3$에 비해 전혀 검출되지 않은 식품이 많았으며, 특히 나물류 11종 중 9종에서 비타민 $B_1$ 성분이 검출되지 않았다. 이상 본 연구의 결과는 향후 식품 영양성분 데이터베이스 구축의 기초자료로 활용될 것이며, 이를 통해 국민 식생활 건강 증진에 기여할 수 있을 것으로 생각된다.

• 한국환경보건학회지
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• 제22권1호
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• pp.36-44
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• 1996

A comparison was made of two detection methods(UV absorbence detection and fluorescence detection with pre-column derivatization, with trifluoroacetic acid) coupled with HPLC for the simultaneous determination of aflatoxin $B_1, B_2, G_1$ and $G_2$. A good separation of the four aflatoxins was achieved on a reversed-phase $C_{18}$ column (30 cm x 3.9 mm) with methanol-acetonitrile-water(20+20+60) for absorbence detection or acetonitrile-water(25+75) for fluorescence detection at the flow rate of 1.0 ml/min. The calibration graphs were linear over the ranges 100 ppb-1 ppm for $B_1/G_1$ and 30~300 ppb for $B_2/G_1$ with absorbence detection, and 1~500 ppb for $B_1/G_1$ and 0.3~150 ppb for $B_2/G_2$ with fluorescence detection. The correlation coefficients were greater than 0.94 and 0.99 for absorbance detection and for fluorescence detection, respectively. The detection limit was 100 ng for $B_1/G_1$ and 30 ng for $B_2/G_2$ with absorbence detection, and 1 ng for $B_1/G_1$ and 0.3 ng for $B_2/G_2$ with fluorescence detection. Recovery rates of aflatoxin $B_1, B_2, G_1$ and $G_2$ added to yeast-extract sucrose broth medium were 66.6%, 59.4%, 67.5% and 59.2%, respectively, for absorbence detection and 82.9%, 71.5%, 80.0% and 69.3%, respectively, for fluorescence detection. The four aflatoxins in culture medium were quantitatively detected by the two methods. The aflatoxins in the rice sample were not detected the absorbence detection method, but were below 10 ppb using the fluorescence detection method. Analysis of aflatoxins by both the absorbence and fluorescence methods coupled with HPLC showed acceptable linearity and good recovery. The absorbence detection was less timeconsuming and safer for treatment. The fluorescence detection was more elective and sensitive though elevated $B_1$ and $G_1$ contents were determined from the TFA-induced conversion of $B_1$ to $B_{2a}$ and $G_1$ to $G_{2a}$.

• 미생물학회지
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• 제49권2호
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• pp.191-194
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• 2013

아위느타리버섯(P. eryngii var. ferulae)의 비타민 $D_2$ (ergocalciferol) 함량 증강을 위하여 아위느타리버섯 자실체에 자외선(UV)-B파장(280-320 nm)을 조사하였다. 비타민 $D_2$ (ergocalciferol)의 증가된 함유량은 HPLC (Waters 1525, USA) 분석으로 확인하였다. 비타민 $D_2$ 함량은 3분($21.6KJ/m^2$) 처리시 $3.5{\mu}g/g$, 5분($36KJ/m^2$) 처리시 $6.02{\mu}g/g$으로 자외선을 조사하지 않은 대조군 $0.01{\mu}g/g$에 비교하여 높은 증가를 나타내었다. 이와 함께 큰느타리버섯(P. eryngii)은 대조군 $0.09{\mu}g/g$, 3분 처리시 $2.75{\mu}g/g$, 5분 처리시 $5.21{\mu}g/g$으로 나타났다. 표고버섯(L. edodes)은 대조군 $0.02{\mu}g/g$, 3분 처리시 $3.02{\mu}g/g$, 5분 처리시 $3.78{\mu}g/g$으로 나타났다. 느타리버섯(P. ostreatus)은 대조군 $0.19{\mu}g/g$, 3분 처리시 $9.63{\mu}g/g$, 5분 처리시 $11.60{\mu}g/g$으로 나타났다. 느타리버섯(P. ostreatus)의 비타민 $D_2$ 함량이 아위느타리버섯, 큰느타리버섯과 표고버섯 보다 높았으나 비타민 $D_2$ 함량 증가율은 아위느타리버섯이 월등히 높았다.