• Korean Journal of Veterinary Service
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• v.41 no.4
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• pp.257-262
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• 2018

Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.241-242
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• 2003

The effects of N-acetylphytospingosine(NAPS), one of the phytospingosine derivatives, on melanogenesis of B 16F 1 0 mouse melanoma cell lines were investigated. We assessed the effect of NAPS on the depigmentation of B16F10 cells. The melanin content of cells was significantly reduced by NAPS. We examined the inhibitory effect of NAPS on tyrosinase activity using L-dopa as a substrate and the results showed that tyrosinase activity was inhibited in a does-dependent manner. The mRNA level of tyrosinase as well as that of tyrosinase related protein-l (TRP-l) and tyrosinase related protein-2 (TRP-2) genes were not affected by NAPS based on a reverse transcription-polymerase chain reaction (RT-PCR) assay. We also performed a Western blotting analysis using anti-tyrosinase antibody. It showed that there is no change in tyrosinase protein level after treatment of NAPS. These results suggest that the depigmenting mechanism of NAPS in B16F10 melanoma cells involves inhibition of melanosomal tyrosinase activity, rather than the mRNA expression or protein level of tyrosinase.

• Journal of Biosystems Engineering
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• v.14 no.2
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• pp.67-79
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• 1989

This study was conducted to investigate the applicability of true model theory for pull prediction in a powered lugged wheel-soil system and to examine the possibility of using principles of similitude in investigating the dimensions of soil parameters pertinent to a powered lugged wheel-soil system concerning the pull prediction. The following conclusions were derived from the study; 1) The pull of prototype wheels proved to be predicted by those of the model wheels for the range of the dynamic weight tested. The pull curves of models and prototype were respectively very similar in the shape. From this basic knowledge, it was enabled to apply the similitude theory to the performance prediction of the true model. 2) A conditional equation which can be used for the prediction of pull of prototype by model test was derived as follows. $n_f=n_1^{-b}$ where $n_f$ : force scale = $w/w_m$ $n_1$ : length scale = ${\ell}/{\ell}_m$ b : exponent on the length dimension of the soil property ${\alpha}$ The range of the numerical value of b, which was determined by the least square method, was found to be -2.0~-2.6. 3) Considering a relatively wide variation of b values in the pull prediction, b is considered to be a function of many variales. Thus it was concluded that there are several soil properties which are pertinent to the powered lugged-wheel-soil system concerning the pull prediction, and these soil properties may have the different effects on the pull of model and protytype wheels, to give the different dimension on the soil parameters.

• Journal of Advanced Marine Engineering and Technology
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• v.24 no.6
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• pp.24-33
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• 2000

This paper investigates the relationship between J-integral and crack tip opening displacement, ${\delta}_t$ using Gordens results of numerical analysis. Estimation were carried out for several strength levels such as ultimate, flow, yield, ultimate-flow, flow-yield stress to determine the influence of strain hardening and the ratio of crack length to width on the $J-{\delta}_t$ relationship. It was found that for SE(B) specimens, the $J-{\delta}_t$ relationship can be applied to relate J to ${\delta}_t$ as follows $J=m_j{\times}{\sigma}_i{\times}{\delta}_t$ where $m_j=1.27773+0.8307({\alpha}/W)$, ${\sigma}_i:{\sigma}_U$, ${\sigma}_{U-F}={\frac{1}{2}} ({\sigma}_U+{\sigma}_F$), ${\sigma}_F$, ${\sigma}_F}$ $Y=({\sigma}_F+{\sigma}_Y)$, ${\sigma}_Y$

• Structural Engineering and Mechanics
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• v.4 no.3
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• pp.227-242
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• 1996

An important class of problems in the field of geotechnical engineering may be analyzed with the aid of a simple integro-differential equation. Behavior of "rigid" piles(say concrete piles), "deformable" piles(say gravel piles), pile groups, pile-raft foundations, heavily reinforced earth, flow within circular silos and down drag on cylindrical structures (for example the crusher unit of a mineral processing complex) are the type of situations that can be handled by this type of equation. The equation under consideration has the form; $$\frac{{\partial}w(r,\;z)}{{\partial}z}+f(z){\int}^z_0g({\xi})(\frac{{\partial}^2w(r,\;{\xi})}{{\partial}r^2}+\frac{1}{r}\frac{{\partial}w(r,\;{\xi})}{{\partial}r})d{\xi}+h(r,\;z)=0$$ where w(r, z) is the vertical displacement of a soil particle expressed as a function of the polar cylindrical space coordinates (r, z) and the symbols f, g and h represent soil properties and the loading conditions. The merit of the analysis is its simplicity (both in concept and in application) and the ease with which it can be expressed in a computer code. In the present paper the analysis is applied to investigate the behavior of a single rigid pile to bedrock. The emphasis, however, is placed on developing the equation, the numerical techique used in its evaluation and validation of the technique, hereafter called the ID technique, against a formal program, CRISP, which uses the FEM.

• Journal of the Korean Ceramic Society
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• v.38 no.11
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• pp.980-986
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• 2001

Bragg reflector type FBAR was fabricated on the Si(100) substrate. We measured a frequency response of the resonator at 5.2 GHz and analyzed it by numerical calculation considering actual acoustic losses of each layer in the structure. We fabricated nine layer Bragg reflector of W-SiO$_2$pairs using r.f. sputtering method and fabricated AlN piezoelectric and Al electrodes using pulsed dc sputtering. The return loss(S$_{11}$) of the fabricated Bragg reflector type FBAR was 12 dB at 5.38 GHz and the series resonance frequency(f$_{s}$) was 5.376 GHz and the parallel resonance frequency(f$_{p}$) was 5.3865 GHz. Effective electro-mechanical coupling constant (K$_{eff{^2}}$) and Quality factors(Q$_{s}$), the Figures of Merit of the resonator, were about 0.48% and 411, respectively. We extracted acoustic parameters of AlN piezoelectric and reflection coefficient of the Bragg reflector by numerical calculation. We could know that material acoustic impedance and wave velocity of AlN piezoelectric decreased for intrinsic value and the electromechanical coupling constant(K$_2$) value was very low owing to the poor quality of the AlN piezoelectric. Reflection coefficient of Bragg reflector was 0.99966 and reflection band was very wide from 2.5 to 9.5 GHz.

• Journal of the Korea institute for structural maintenance and inspection
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• v.5 no.4
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• pp.131-138
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• 2001

In inverted T-shape composite beam examine the structural behavior by experiment According to arrangement, type of stud connector, type of shear connector, reinforcement by welding of stirrup and a close analysis we came to these conclusion. 1) The compare result of bending strength according to arrange type of stud connector : A1-W(stud connector located web)specimen is exceed than A1-F(stud connector located flange). 2) B1-N(using prominence and depression of the web by shear connector) specimen is decrease than A1-W(using stud bolt by shear connector)specimen in bending strength and B2-N(reinforced by welding the stirrup to lower flange)specimen is similar with A2-W specimen. 3) According to reinforced by welding the stirrup to flange, the stiffness and bending strength of the beams are increase. A-scries stirrup comparatively low effective in the increase of strength by welding the stirrup to flange because enough composite effect show by stud connector, but B-series stirrup is comparatively high effective in shear connector effect because shortage of prominence and depression of the web.

• Journal of the Korean Society for Heat Treatment
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• v.22 no.5
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• pp.307-311
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• 2009

In this study, TiCrN films were deposited on STS 316 Land Si (100) wafer by inductively coupled plasma (ICP) assisted D.C. magnetron sputtering. The effect R.F. power for ICP discharge on the mechanical properties of TiCrN films was investigated. XRD, XPS and FE-SEM were used for the structure analysis. Also the Micro-Knoop hardness tester and profilometer were used for measuring hardness of coatings and film stress respectively. As increasing the R.F. power for ICP discharge, thickness of coating was decreased from 1633 nm to 1288 nm but hardness was increased about $Hk_{5g}$ 4200 at 400 W. All of the XRD patterns showed (111), (200) and (220) peaks of TiCrN films. Surface morphology was studied using the profilometer. FE-SEM was used to know morphology and cross-section of the films. Structure of the films was changed dense as increased ICP power.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.690-693
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• 2005

In this paper, probabilistic distribution of fatigue life of chassis component is determined statistically by applying the design of experiments and the Pearson system. To construct $p-\varepsilon-N$ curve, the case that fatigue data are random variables is attempted. Probabilistic density function(p.d.f) for fatigue life is obtained by design of experiment and using this p.d.f fatigue reliability about any aimed fatigue life can be calculated. Lower control arm and rear torsion bar of chassis component are selected as examples for analysis. Component load histories, which are obtained by multi-body dynamic simulation for Belsian load history, are used. Finite element analysis are performed using commercial software MSC Nastran and fatigue analysis are performed using FE Fatigue. When strain-life curve itself is random variable, probability density function of fatigue life has very little difference from log-normal distribution. And the case of fatigue data are random variables, probability density functions are approximated to Beta distribution. Each p.d.f is verified by Monte-Carlo simulation.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1184-1191
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• 2011

We attempted to generate a linkage map using Illumina Porcine 60K SNP Beadchip genotypes of the $F_2$ offspring from Korean native pig (KNP) crossed with Yorkshire (YS) pig, and to identify quantitative trait loci (QTL) using the line-cross model. Among the genotype information of the 62,136 SNPs obtained from the high-density SNP analysis, 45,308 SNPs were used to select informative markers with allelic frequencies >0.7 between the KNP (n = 16) and YS (n = 8) F0 animals. Of the selected SNP markers, a final set of 500 SNPs with polymorphic information contents (PIC) values of >0.300 in the $F_2$ groups (n = 252) was used for detection of thirty meat quality-related QTL on chromosomes at the 5% significance level and 10 QTL at the 1% significance level. The QTL for crude protein were detected on SSC2, SSC3, SSC6, SSC9 and SSC12; for intramuscular fat and marbling on SSC2, SSC8, SSC12, SSC14 and SSC18; meat color measurements on SSC1, SSC3, SSC4, SSC5, SSC6, SSC10, SSC11, SSC12, SSC16 and SSC18; water content related measurements in pork were detected on SSC4, SSC6, SSC7, SSC10, SSC12 and SSC14. Additional QTL of pork quality traits such as texture, tenderness and pH were detected on SSC6, SSC12, SSC13 and SSC16. The most important chromosomal region of superior pork quality in KNP compared to YS was identified on SSC12. Our results demonstrated that a QTL linkage map of the $F_2$ design in the pig breed can be generated with a selected data set of high density SNP genotypes. The QTL regions detected in this study will provide useful information for identifying genetic factors related to better pork quality in KNP.