The purpose of this study was carried out to present the basic data for constructing a ecological stream, Shindangcheon stream located in Gyeongju-si, Gyeongsangbuk-do, South Korea. From August 2017 to June 2019, a total of 7 surveys were conducted. The numbers of vascular plants were summarized as 388 taxa including 75 families, 246 genera, 360 species, 4 subspecies, 19 varieties, 2 forms and 3 hybrids. The most families of taxa were Poaceae (59 taxa), Asteraceae (47 taxa), Fabaceae (27 taxa), Rosaceae and Cyperaceae (20 taxa, respectively), and Polygonaceae and Brassicaceae (15 taxa, respectively). The rare plant was Aristolochia contorta Bunge, and the Korean endemic plants were 4 taxa, such as Populus × tomentiglandulosa T.B.Lee, Salix koriyanagi Kimura ex Goerz, Lespedeza maritima Nakai, and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region found in this site were 13 taxa comprising 8 taxa of grade I, 4 taxa of grade III, and 1 taxon of grade IV. The naturalized plants were 73 taxa, such as Fallopia dumetorum (L.) Holub, Persicaria orientalis (L.) Spach., Potentilla supina L. var. ternata Pterm., and so forth. The invasive alien plants were 6 taxa, such as Rumex acetosella L., Sicyos angulatus L., Solanum carolinense L., Ambrosia artemisiifolia L., Lactuca serriola L., and Symphyotrichum pilosum (Willd.) G.L.Nesom..