• 한국잠사곤충학회지
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• 제45권1호
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• pp.18-24
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• 2003

한반도에 서식하는 멧누에나방의 서식지역에 따른 유전적 변이와 집누에나방과의 종간 유연관계를 조사하였다. 멧누에나방의 isozyme 및 체액단백질을 대상으로 유전자 빈도를 조사한 결과, 조사지역 중 경북 칠곡과 경남 진주집단 사이의 유전적 유사도가 가장 높았으며, 경남 진주와 강원 고성집단 사이에서 가장 낮게 나타났다. 그러나 서식집단 상호간의 유사도는 매우 높았다. 한국산 멧누에나방과 집누에나방의 isozyme 유전자형은 대부분이 동일하게 발현되었으나, 집누에나방의 한국종에서만 발현된다고 알려진 Bes의 B형, Amy-hc의 F형, Ict-E의 n형 및 Ict-h의 M형과 S형 등의 유전자형이 한반도에 서식하는 멧누에나방에서 발현되었다. 따라서, 한반도 내에서 멧누에나방의 서식지역간 유전적 변이는 인정되지 않으며, 지금까지는 고려되지 않았던 집누에 한국종과 한국산 멧누에나방의 종간 유연관계에 대해 새로운 의문이 야기된다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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• pp.42-42
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• 2003

The wild silkworm, Bombyx mandarina, was believed the only ancestor of B. mori, inhabits the limited area of Eastern Asia including China, Korea and Japan. However, the geographic dimorphism of B. mandarina was reported with chromosome number and arylphorin gene. In connection with those dimorphism, we studied the genetic differences of ITS-2 region in rDNA purposing the differentiation and geographic variation within the species of B. mandarina. (omitted)

• 한국잠사곤충학회지
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• 제27권1호
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• pp.37-41
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• 1985

이 연구는 polyacrylamide gel 전기영동법을 사용해서 상잠의 5령 3일째 유충 및 가잠의 5령 3일째 유충에 있어서 혈액, 소화액 단백질의 전기 영동상과소화액 amylase 활성을 조사하였다. 1. 상잠의 혈액에 있어서 자유충에서는 6본의 주요 단백질 band가 검출되었으며 웅유충에서는 7본의 주요 단백질 band가 검출되었다. 그러나, 가잠에 있어서 암누에에 있어서는 8본, 숫누에에 있어서는 7본이 검출되었다. 상잠과 가잠과의 혈액단백질의 전기영동상에 있어서 다소의 차이가 있었다. 2. 상잠의 소화액에서 15본의 단백질 band가 검출되었으며 가잠에서는 12본의 단백질 band가 검출되었다. 상잠과 가잠과의 소화액 단백질의 전기영동상에 있어서도 다소의 차이가 있었다. 3. 상잠의 소화액 amylase는 가잠과 같이 양극측 BPB 부근까지 강하게 이동하였으며 상잠의 소화액 amylase의 이동도는 0.019이고 가잠의 이동도는 0.020으로서 양종간의 이동도의 차이가 거의 없었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.113-120
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• 2016

Bombyx mandarina is widely accepted as ancestor of B. mori. Silkworms are served as well-characterized models for understanding the mechanism for the genetic regulation of development. In this study, we performed RNA-Seq analysis to examine tissue-expression of gloverin isoforms of the silk-gland, mid-gut, and fat body in B. mandarina. BLAST analysis revealed that four gloverin isoform gene sequences of B. mandarina were highly similar to B. mori. To identify the difference between two species, the expression profile of gloverin was measured by semi- RT-PCR analysis. The specific expression of gloverin isoform genes was observed mainly in the fat body from B. mori but not B. mandarina. However, all of tissues in the wild-type silkworm could induce the upregulation of compared with the B. mori. To validate the sudden increase in gloverin gene expression in the mid-gut tissue of B. mandarina, we were using qRT-PCR. Relative mRNA expression rate of gloverin at the wild-type silkworm was much higher than domestic silkworm. Comparative genomics between domesticated and wild silkworms showed different tissue-expression levels in some of immune related genes. These results are suggesting a trend toward decreasing immunity related genes expression during domestication. Further studies are needed to elucidate the silkworm domestication and an invaluable resource for wild silkworm genomics research.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권1호
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• pp.9-17
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• 2000

Genetic variation in the domestic silkworm strains (Bombyx mori) and phylogenetic relationships between domestic silkworms and wild silkworms (B. mandarina) were investigated by using a portion of mitochondrial CGI gene sequences. Ten geographic strains of B. mori we sequenced were identical in the 410 bp-section of mitochondrial COI gene. This sequence was also identical to the homologous sequence of the four Gen-Bank-registered strains, but one strain of B. mori differed a single nucleotide (0.2%) from others. MtDNA homogeneity in the B. mori strains appears to be resulted from fixation into the mast frequent mtDNA type during the course of breeding for new strains, in which an extensive indoor rearing and removal of unwanted individuals were accompanied. In the comparisons between domestic and wild silkworms, some wild silkworms were closely related to domestic silkworms (0.2%-1.2% of divergence), but the others were not (2.7%-3.7% of sequence divergence). This result was also reflected in the phylogenetic analyses, showing two independent phylogenetic groups: one including all B. mandarina sequences and the other including both B. mandarina and B. mori sequences. Thus, domestic silkworms may have been derived from the ancestor of B. mandarina, which belongs to this group, alto-ough more extensive study will provide better understanding on this issue.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.101-106
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• 2005

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.147-153
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• 1999

Chitinolytic enzymes such as ${\beta}$-N-acetylglucosaminidase are major hydrolases involved in insect molting. We have isolated, sequenced a CDNA encoding ${\beta}$-N-acetylglucosaminidase from the silkworm, Bombyx mandarina, and compared its sequence with genes encoding chitinolytic enyzmes from other sources. The insert DNA in the clone is 3,284 nucleotides long with an open reading frame of 1,788 nucleotides that encodes a protein of 596 amino acids with a molecular weight of 68.2 kDa. There is a 3’-untranslated region composed with 1.479 nucleotides and are several potential polyadenylation signals. The predicted amino acid sequence apparently contains a leader peptide of 23 amino acids. A search of the amino acids sequence databases for sequences similarities to other ${\beta}$-N-acetylglucosaminidases or ${\beta}$-N-acetylhexosaminidases. The highest similarity matched with the enzyme from B. mori, which has a sequence identity of 95%. On the other hand, the identity between the B. mandarina enzyme and those from M. sexta and human are 70% and 24%, respectively.

• Journal of Life Science
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• 제9권1호
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• pp.84-89
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• 1999

To determine phylogenetic relatedness of four silk-producing silkmoths (B. mori, B. mandarina, A. yamamai and A. pernyi), internal coding region of arylphorin which is a storage protein in hemolymph protein of insects were amplified by polymerase chain reaction and then sequenced and compared each other. The nucleotide composition was biased toward adenine and thymine(59% A+T) and a strong bias for use of C in the third position of codons was found for Phe and Tyr. Together TTC(Phe) and TAC(Tyr) account for about 16.8% (10 for TTC and 8 for TAC) of all codon usage. The nucleotide similarity of arylphorin gene from B. mori showed 99%, 98% and 97% homology with those of B. mandarina, A. yamamai and A. pernyi, respectively. Also, the nucleotide sequence of arylphorin gene from B. mandarina showed 98% and 97% homology with those of A. yamamai and A.pernyi, respectively. Between A. yamamai and A. pernyi, the sequence homology was 97%. The deduced amino acid sequences in B. mori, B. mandarina and A. yamamai showed almost 99% homology. Although the aryphorin gene provided insufficient variability among the four insect species, A UPGMA tree is generated that supported the monophyly of silk-producing insects, with M. sexta placed basal to it. It is suggest that silk-producing insects have a close relationship and a homogeneous genetic background from comparison with those of other insects.

• 생명과학회지
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• 제9권4호
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• pp.341-347
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• 1999

Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-$\beta$-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.19-20
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• 2002

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