• Journal of Plant Biology
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• 제37권1호
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• pp.77-83
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• 1994

종자를 자연건조시킨 상태에서 ${\beta}-glucuronidase$ (GUS) 유전자와 hygromycin phosphotransferase (HPT) 유전자를 가진 plasmid DNA 용액을 imbibition시켰다. GUS 유전자의 경우 품종, vector의 종류, imbibition의 온도에 따라 표지유전자의 발현율에 차이가 있었으며 약 30-50%의 transient expression을 나타내었다. Hygromycin B (HmB)배지에서 선별된 개체의 genomic DNA를 뽑아 외부유전자의 존재를 dot 분석을 통하여 확인하였다. Inverse polymerase chain reaction 결과 만들어지는 생성물을 cloning하고 sequencing한 결과 CaMV35S promoter sequence를 찾았다. Hygromycin이 첨가된 배지에서 선별된 개체들에서 GUS 유전자의 primer를 이용하여 PCR를 수행한 결과 20개체 중 18개체에서 GUS 유전자가 안정되게 존재하여 HmB 배지에서 GUS 유전자의 존재비율은 90%였다. 본 연구의 결과로부터 두 개의 유전자를 소유한 pYJH vector system이 고등식물의 형질전환에 유용하게 이용될 수 있음을 알 수 있었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2006년도 춘계학술대회 논문집
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• pp.91-94
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• 2006

In this paper, in order to get micro or nano size optical patterns, an analytical and experimental investigation on a LGP (light guide plate) injection molding process has performed. The LGP, which diffusing and emitting the light from the CCFL or the LEDs to the panel front direction uniformly, typically has an under 1mm thick base substrate and numerous 60 to $170{\mu}m$ width and 6 to $10{\mu}m$ thick dot patterns on it. Generally, the small size LGPs, for mobile devices, have been and are being made of PMMA through the injection molding process. However, the substrate thickness and the dot pattern size are decreasing, it becomes hard to fill the micro to sub-micro cavities completely. To investigate the flow behavior of resin in micro/nano cavities and identify the characteristics of the LGP injection molding process, we carried out the flow analyses with respect to the variations of the substrate thickness, the dot pattern size and the pitch of a cavity.

• 한국산업보건학회지
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• 제21권1호
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• pp.49-54
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• 2011

In order to develop the methods for exposure assessment and find susceptibility markers for the workers who are exposed to low doses of toluene, xylene and other chemical in petroleum industries, we investigated the application of P-450 expression in human lymphocytes utilizing mouse monoclonal anti-rat CYP2B1/2, the levels of toluene and xylene in air and their metabolite levels in urine with the levels of expressed CYP2B1/2 proteins. The general characteristics such as age, smoking and drinking habit were no significant difference between the control and exposed workers, but the working durations and working hours were significantly different. Workers in exposed group were exposed to the mean of 2.1 ppm (range, 0.00-4.54) of toluene and 0.3 ppm (rang, 0.00-1.23) of xylene. The mean concentration of urinary hippuric acid was low and less than 1/5 of the biological exposure index recommended by the Ministry of Employment and Labor Korea. Methyl hippuric acid in urine was not detected in control and exposed workers. Also, there were no significant differences in the levels of the urinary metabolites between the control and exposed group. When chemiluminescence dot blottings were carried out utilizing mouse monoclonal antibody against CYP2B1/2, the strong density dots corresponding to a mouse monoclonal antibody was observed in the human lymphocytes from the exposed workers. These results suggested that the chemiluminescence dot blot assay for CYP of lymphocytes should be valuable for identifying CYP expression as biomarkers in the workers exposed to toluene and xylene.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 춘계학술대회 논문집
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• pp.363-364
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• 2009

• 전자공학회논문지B
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• 제30B권8호
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• pp.8-15
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• 1993

This paper describes a normalization of character which is contained in the character recognition process. Line and dot density is computed on input character image and then image mapping is executed into destination. Also recognition is processed using overlap-partitioning of character image and extraction of 4 directional feature primitives. The validity of proposed nonlinear normalization algorithm could be verified by increment of recognition rate.

• 전자공학회논문지B
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• 제31B권12호
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• pp.124-131
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• 1994

In this paper, we propose a new approach of solving the stereopsis problem with a discrete-time cellular neural network(DTCNN) where each node has connections only with its local neithbors. Because the matching process of stereo correspondence depends on its geometrically local characteristics, the DTCNN is suitable for the stereo correspondence. Moreover, it can be easily implemented in VLSI. Therefore, we employed a two-layer DTCNN with dual templates, which are determined with the back propagation learning rule. Based on evaluation of the proposed approach for several random-dot stereograms, its performance is better than that of the Marr-Poggio algorithm.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2002년도 추계 학술대회논문집
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• pp.84-87
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• 2002

When computing the flow around complex three dimensional configurations, the generation of grid is the sunt time consuming part of any calculation. The object of this study is to develop the grid duster techniques capable of resolving complex flows with shock waves, expansion waves, shear layers, and cursive shapes, The Dot insert method of Non-Uniform Rational B-Splines is described as a id control method.

• Journal of information and communication convergence engineering
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• 제14권1호
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• pp.35-39
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• 2016

In this paper, we develop a 94-GHz single balanced mixer with low conversion loss using planar Schottky diodes on a GaAs substrate. The GaAs Schottky diode has a nanoscale anode with a T-shaped disk that can yield high cutoff frequency characteristics. The fabricated Schottky diode with an anode diameter of 500 nm has a series resistance of 21 Ω, an ideality factor of 1.32, a junction capacitance of 8.03 fF, and a cutoff frequency of 944 GHz. Based on this technology, a 94-GHz single balanced mixer was constructed. The fabricated mixer shows an average conversion loss of -7.58 dB at an RF frequency of 92.5 GHz to 95 GHz and an IF frequency of 500 MHz with an LO power of 7 dBm. The RF-to-LO isolation characteristics were greater than -32 dB. These values are considered to be attributed to superior Schottky diode characteristics.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2006년도 한국우주과학회보 제15권1호
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• pp.121-124
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• 2006

B-dot 로직은 일반적으로 위성의 초기각속도 제어에 사용되나, 상대적으로 제어시간이 많이 소요된다는 단점이 있다. 이런 문제를 해결하기 위해 본 논문에서는 피치 바이어스 모맨텀 방식을 사용하는 초소형 위성에 적용 가능한 디텀블링(detumbling) 방식을 새롭게 제안하였다. 제안된 디텀블링 방식은 제어시간이 약 20분 이내로 기존의 방식에 비해 상당한 시간을 줄일 수 있다.

• 대한기계학회논문집B
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• 제30권4호
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• pp.287-297
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• 2006

The Holographic Particle Velocimetry system can be a promising optical tool for the measurements of three dimensional particle velocities. One of inherent limitations of particle holography is the very long depth of field of particle images, which causes considerable difficulty in the determination of particle positions in the optical axis. In this study, we introduced three auto-focusing parameters corresponding to the size of particles, namely, Correlation Coefficient, Sharpness Index, and Depth Intensity to determine the focal plane of a particle along the optical axis. To investigate the suitability of the above parameters, the plane image of dot array screens containing different size of dots was recorded by diffused illumination holography and the positions of each dot in the optical axis were evaluated. In addition, the effect of particle position from the holographic film was examined by changing the distance of the screen from the holographic film. All measurement results verified that the evaluated positions using suggested auto-focusing parameters remain within acceptable range of errors. These research results may provide fundamental information for the development of the holographic velocimetry system based on the automatic image processing.