• The Korean Journal of Physiology
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• 제29권2호
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• pp.233-241
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• 1995

The nonapeptide bradykinin has been shown to exhibit an array of biological activities including relaxation/contraction of various smooth muscles. In order to investigate the effects of bradykinin on the contractility and the electrical activity of antral circular muscle of guinea-pig stomach, the isometric contraction and membrane potential were recorded. Also, using standard patch clamp technique, the $Ca^{2+}-activated$ K currents were recorded to observe the change in cytosolic $Ca^{2+}$ concentration. $0.4 {\mu}M$ bradykinin induced a triphasic contractile response (transient contraction-transient relaxation-sustained contraction) and this response was unaffected by pretreatment with neural blockers (tetrodotoxin, atropine and guanethidine) or with apamin. Bradykinin induced hyperpolarization of resting membrane potential and enhanced the amplitude of slow waves and spike potentials. The enhancement of spike potentials was blocked by neural blockers. Both the bradykinin-induced contractions and changes in membrane potential were reversed by the selective $B_2$-receptor antagonist $(N{\alpha}-adamantaneacetyl-_{D}-Arg-[Hyp, Thy,_{D}-Phe]-bradykinin)$. In whole-cell patch clamp experiment, we held the membrane potential at -20 mV and spontaneous and transient changes of Ca-activated K currents were recorded. Bradykinin induced a large transient outward current, consistent with a calcium-releasing action of bradykinin front the intracellular calcium pool, because such change was blocked by pretreatment with caffeine. Bradykinin-induced contraction was also blocked by pretreatment with caffeine. From these results, it is suggested that bradykinin induces a calciumrelease and contraction through the $B_{2}$ receptor of guinea-pig gastric smooth muscle. Enhancement of slow wave activity is an indirect action of bradykinin through enteric nerve cells embedded in muscle strip.

• 한국융합학회논문지
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• 제12권3호
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• pp.281-286
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• 2021

본 연구는 여성의 브래지어 착용 여부와 가슴 크기에 따른 흉곽 가동성과 가로막의 움직임 및 폐활량을 비교해보고자 하였다. 충남 B 대학에 재학 중인 10명을 대상으로 실험하였으며 속옷의 사이즈가 75A인 5명을 A그룹, 85B인 5명을 B그룹으로 나누어 실험을 진행하였다. 브래지어 착용 전후 원형 줄자를 이용하여 흉곽 가동성을, 초음파를 이용하여 가로막의 움직임을 측정하였으며 폐활량 측정을 위하여 Pony FX를 이용하였다. 브래지어 착용 여부를 비교한 결과, A그룹과 B그룹 모두에서 브래지어를 착용하였을 때 흉곽 가동성과 가로막 움직임이 유의하게 감소하였지만. 폐활량은 A 그룹에서만 유의하게 감소하였다. A그룹과 B그룹을 비교한 결과, 모든 종속변수에서 유의한 차이는 없었다. 그러므로 본 연구는 가슴 크기에 상관없이 브래지어 착용 자체만으로도 흉곽 가동성과 가로막의 움직임에 부정적 영향을 끼칠 수 있음을 시사한다.

• 대한약리학회지
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• 제8권2호
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• pp.55-61
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• 1972

Superprecipitation of actomyosin has been considered to be an in vitro model of the muscle contraction. The superprecipitation and ATPase activity (which supplies the energy for contraction) are influenced by several factors which are the large amount of changes in ionic strength, Mg and ATP concentrations. But those behaviors are found to be promptly influenced by the change in a small range of calcium concentration which can be controlled by the cellular function of muscle physiologically only in the presence of the modullatory proteins, tropomyosin and troponin. In order to elucidate the precise roles of calcium in the muscle contraction and relaxation, the effects of calcium on the actin- myosin interaction was observed in the presence of tropomyosin and troponin using the superprecipitation system. The results are summarized as follows: 1. EGTA (glycol ether diaminetetraacetic acid)prolonged the initiation of the superprecipitation of natural actomyosin. 2. Superprecipitation curve was declined by adding EGTA at the time when tile curve reached the half- maximum. The degree of declining was proportional to the amount of EGTA added. Especially, upon adding 0.25 mM EGTA the curve was lowered to the level before the protein superprecipitated. But addition of EGTA did not affect the curve after attaining the maximum. 3. Superprecipitation of Perry myosin B was not affected by EGTA added both before and during the course of the reaction. 4. Tropomyosin did not change the response of Perry myosin B to EGTA added at any time of the reaction. 5. Troponin also did not change the response of Perry myosin B to EGTA. 6. Both tropomyosin and troponin together rendered the Perry myosin B to obtain the same response as natural actomyosin to EGTA. 7. It was concluded that actin-myosin interaction was influenced by the minute change of calcium concentration only in the presence of both tropomyosin and troponin. We could reproduce the contraction and relaxation of the muscle in vitro under the presence of ATP by changing the calcium concentration.

• The Korean Journal of Physiology
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• 제30권2호
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• pp.269-278
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• 1996

The mechanism of impaired endothelium-dependent relaxation in the aorta of one-kidney, one clip Goldblatt hypertensive (1K,1C-GBH) rats was investigated. 8 week-old Wistar-Kyoto (WKY) rats were made hypertensive by left renal artery stenosis with contralateral nephrectomy. Endothelium-dependent relaxation was significantly reduced in 1K,1C-GBH rats as compared with WKY rats. However, the relaxation by sodium nitroprusside in 1K,1C-GBH rats was not reduced as compared with WKY rats. The impairment of endothelium-dependent relaxation in 1K,1C-GBH rats was partially restored by the pretreatment of indomethacin or SQ29548. When the nitric oxide production was inhibited by L-nitroarginine methyl ester, acetylcholine (ACh) induced a endothelium-dependent contraction that was greater in 1K,1C-GBH rats than in WKY rats. Endothelium-dependent contraction by ACh was completely abolished by indomethacin or SQ29548. However, imidazole, tranylcypromine and superoxide dismutase did not affect the endothelium-dependent contraction in 1K,1C-GBH rats. These results suggest that impaired endothelium-dependent relaxation in the 1K,1C-GBH rats might be due to the simultaneous release of EDCF, and that prostaglandin B2 may be involved as a mediator of endothelium-dependent contraction.

• 대한정형도수물리치료학회지
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• 제29권2호
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• pp.69-75
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• 2023

Purpose: This study examined the distance between the coracoid process and the humeral head using an ultrasonography device when shoulder active contraction were applied according to the guided direction in the end range of shoulder mobilization. This study aims to provide essential data on treating shoulder disease patients. Methods: The subjects of this study were 20 adults with healthy shoulder joints. ultrasonography (US) equipment was used to examine shoulder joint mobilization under two conditions: (1) anteroposterior (AP) joint mobilization and (2) superoinferior (SI) joint mobilization. Shoulder active contraction was assessed in the end range. The distance between the coracoid process and the humeral head was measured. A linear probe was used for US; the frequency was set to 7.5MHz, and the US image display method was set to B-mode. The US measurement values were measured in (1) the starting position, (2) the end range position, and (3) the end range position of the shoulder active contraction, and the moving distance was drawn in a straight line through the US image. The distance was determined as the measurement value, and the average values were compared. Reults: The results were as follows: (1) the measured AP Joint mobilization increased by an average of .52cm from the end range of the joint mobilization with shoulder active contraction; (2) the measured SI Joint mobilization increased by an average of .49cm from the end range of the joint. Conclusion: When shoulder mobilization is applied, the distance between the coracoid process and the humeral head increases when muscle contraction occurs through shoulder active contraction in the end range, according to the therapist's guidance. Therefore, shoulder mobilization combined with shoulder active contraction is an effective treatment method for patients with shoulder injuries.

• 생약학회지
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• 제17권4호
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• pp.292-301
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• 1986

The combined preparations of the traditional Korean prescriptions, Sahyangsohap-won (A1) and Woohwangporyong-hwan (B1) were evaluated on pharmacological actions in rats and mice, in parallel with the preparations of their modified prescriptions (A2 and B2). The acute toxicities of the four preparations were very low of which $LD_{50}$ values were more than 4g/kg, p.o. and no systematic symptoms were found at the doses. All the preparations showed sedative action by prolongation of sleeping time induced with hexobarbital sodium. The action was more potent in the modified preparations than in the original ones. All the preparations had no anticonvulsant action both in chemoshock seizures induced by pentetrazole and strychnine and in electroshock seizure. In rat fundus preparation, A1 and A2 elicited strong contraction at the concentration of $1{\times}10^{-3}\;g/ml$ in bath, whereas B1 and B2 did neither contraction nor relaxation at the same concentration. The four preparations had no inhibitory effect against acetylcholine induced spasm. In rat intestinal preparation, the four preparations exhibited neither contraction nor relaxation. However, A1 and A2 had antagonistic effect against spasm at the concentration of $1{\times}10^{-3}\;g/ml$. Single administration of each preparation at the dose of 0.24g/kg, p.o. stimulated the secretion of pepsin in rat stomach without inciting the secretion of gastric juice. Their stimulating actions were more marked in B1 and B2 than in A1 and A2, and were more promptly exhibited in the modified preparations (A2 and B2). Accelerating action of bile secretion by single administration of each preparation was found at the dose of 0.24g/kg, p.o. in rats. All the preparations except A2 also stimulated the secretion of bile acid.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제22권3호
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• pp.199-214
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• 2015

We establish a common coupled fixed point theorem for hybrid pair of mappings under generalized Mizoguchi-Takahashi contraction on a noncomplete metric space, which is not partially ordered. It is to be noted that to find coupled oincidence point, we do not employ the condition of continuity of any mapping involved therein. An example is also given to validate our results. We improve, extend and generalize several known results.

• 대한약리학회지
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• 제26권1호
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• pp.13-23
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• 1990

가토흉부대동백근에서 MeB와 gentian violet는 용량-의존성 수축반응을 일으켰으나 evans blue와 eosine yellowish는 전혀 수축반응을 일으키지 못하였다. MeB는 돼지 장간막 동맥에서도 용량-의존성 수축반응을 일으켰다. 양표본에서 MeB $10^{-4}$ M의 단회투여는 수축반응에 이어 이완반응이 나타나는 양상성 반응을 일으켰으나, tyramine은 지속적인 수축반응을 일으켰다. Tyramine의 수축반응은 반복적이었으나 MeB의 그것은 일차 수축반응후 $3{\sim}5$시간후까지도 반복되지 않았다. Tyramine $10^{-4}$ M의 최대 수축반응 상태에서 MeB $10^{-4}$ M의 추가투여는 현저한 추가수축반응을 일으켰으나 반대로 MeB의 최대수축반응 상태에서 tyramine의 추가투여는 그이상의 수축반응을 일으키지 못하였다. Tyramine과 MeB 수축반응은 교감신경계 악물로 소실 또는 유의하게 억제되었다. Tyramine 수축반응은 MeB 수축보다 guanethidine과 6-hydroxydopamine에 더 예민한 반면, $Ca^{2+}-free$ PSS와 reserpine에 대하여는 MeB 수축반응이 tyramine 수축보다 더 예민하였고 prazosin하에서는 두 수축반응이 비슷하게 억제되었다. MeB 수축반응은 6-hydroxydopamine으로 유의하게 억제는 되었으나 소실되지 않았고, MeB 수축반응 관찰후에는 tyramine 뿐만아니라 6-hydroxydopamine의 수축반응도 소실되었다. 이상의 성적은 가토흉부대동맥과 돼지 장간막동맥에서 MeB 수축반응은 부분적으로 세포외 calcium 의존성이고 adrenaline성 신경발단으로부터의 norepinephrine유리에 기인하며, MeB의 norepinephrine유리 및 고갈작용이 tyramine 또는 6-hydroxydopamine의 작용보다 더 강력함을 시사하고 있다.

• 한국융합학회논문지
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• 제7권1호
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• pp.105-111
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• 2016

본 연구에서는 실시간 초음파 영상 분석을 통해 세 가지 골반바닥근 수축 운동이 골반바닥근과 배가로근, 배속빗근, 그리고 배바깥빗근의 두께에 미치는 영향을 분석하였다. 골반바닥근과 몸통 근육들의 근두께는 초음파 영상 장비를 이용하여 안정시, 전통적 골반바닥근 수축[운동 A]시, 엉덩 모음근과 골반바닥근 동시 수축[운동 B]시, 실시간 초음파 영상장비를 이용한 골반바닥근 수축 유도[운동 C])시 측정하였다. 측정 결과, 다른 운동들에 비해 운동 C 수행 시 안정 시 보다 가장 크게 골반바닥근의 두께가 감소하였고(p<.05), 배가로근 역시 다른 운동들에 비해 운동 C 수행 시 안정 시 보다 근두께가 가장 크게 증가하였다(p<.05). 이와 같은 결과를 바탕으로, 본 연구에서 수행한 세 가지 골반바닥근 수축 방법들 중에서 실시간 초음파 영상 장비를 이용하여 골반바닥근 수축을 유도하는 운동 C 방법이 골반바닥근 수축을 통한 여성들의 오줌새기를 개선시킬 수 있는 방법으로 추천될 수 있을 것으로 사료된다.

• 유변학
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• 제2권1호
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• pp.33-45
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• 1990

This study analyzes the planar 4:1 contraction flow of viscoelastic fluids with retardation time using finite volume method. To consider separately the elasticity effect of the viscoelastic fluid without shear thinn-ing effect, Oldroyd B liquid model is adopted for the numerical simulation. Instead of the stream function-vorticity formulation, SIMPLER algorithm with staggered grid system which incorporates primitive variable has been introduced in discretizing the momentum equations. An upwind corrected scheme has been used in discetizing the constitutive equations for the non-Newtonian part of the stress. The size of the corner vortex is shown to be slightly influenced by the Weissenberg number. However as the Weissenberg number is increased the chang-ing of the vortex shape agrees qualitatively well with some experimental studies.