• The Journal of Internal Korean Medicine
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• v.28 no.4
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• pp.797-807
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• 2007

Objectives : The purpose of this study was to investigate whether Mahwangyunpye-tang(MYT) significantly affects mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of MYT to assess the effect of the agent on 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed. Effect of MYT on contractility of isolated tracheal smooth muscle was investigated; also investigated was effect of the agent on MUC5AC gene expression in cultured NCI-H292 cells. Possible cytotoxicities of the agent were assessed both by measuring lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : MYT significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity. MYT chiefly affected the 'mucin' secretion. MYT inhibited Acetylcholine-induced contraction of isolated tracheal smooth muscle. MYT did not significantly affect the expression levels of MUC 5AC gene in cultured NCI-H292 cells. Conclusions : Based on the above results, it is suggested that MYT increased mucin secretion from cultured HTSE cells without significant cytotoxicity and inhibited contraction of isolated tracheal smooth muscle.

• Archives of Pharmacal Research
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• v.14 no.2
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• pp.181-187
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• 1991

The muscarinic antagonist 1-[benzilic 4, 4'-$[^3H]$ QUINUCLIDINYL BENZILATE $([^3H]$ QNB) bound to a single class of muscarinic receptors with high affinity in rabbit ileal membranes. The $K_D\;and\;B_{ max}$ values for $([^3H]$ QNB calculated from analysis of saturation isotherms were 52.5 pM AND 154 fmol/mg, respectively. Chlopheniramine (CHP), histamine $H_1$ blocker, increased $K_D$ vlue for $([^3H]$QNB without affecting the binding site concentrations and Hill coefficient. The $K_i$ value of CHP for inhibition of $([^3H]$QNB binding in ileal membranes was 1.44\mu{M}$ and the pseudo-Hill coefficient for CHP was close to unit. In the functional assay carbachol, muscarinic agonist, increased the contractile force of ileum with $ED_{50}$ value of $0.11\mu{M}$. CHP caused the rightward shift of the dose-response curve to carbachol. The $pA_2$ value of CHP determined from Schild analysis of carbacholinduced contraction was 5.77 and the slope was unity indicating competitive antagonism with carbachol. The dissociation constant $(K_i)$ of CHP obtained in competitive experiments with $([^3H]$ QNB was similar to the $K_A$ value (1.69 \mu{M)}$ of CHP as inhibitor of carbachol induced contraction in rabbit ileum. This result suggest that the binding of $H_i$ blocker. CHP, vs $([^3H]$QNB to muscarinic receptors in ileal membranes represents an interaction with a receptor of physiological relevance.

• Journal of the Korean Home Economics Association
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• v.19 no.3
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• pp.21-32
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• 1981

Clothing must have the individual beauty and the function that one can do one's body movement freely. therefore, from the human engineering view point, the exact measurement of the human body and the analysis of it's results must be applied to clothing because the arm works most. In this study, the skin surface of arm was investigated by shell made of Alginate of each movement. And by sometic method the rate of expansion and contraction of each section, inter-relation among the bust girth, axillary arm girth and scye girth and between the shoulder length right and the under axilla waist length were calculate4d. With these results, cap height, breadth of a sleeve and arm hole girth of existing sleeve pattern have been compared and analysed. The results are as follows; 1) According to the developmental figures of shell, the whole area change was small but that of shape was remarkable in M 4, 9, 13. The change of the Block 1 was particular. 2) Over arm length was contracted in all movements except M6 and under arm length expanded. The changes of sections a, b, c are large, while those of sections d, e are small. 3) In terms of latitudes, change of fore-arm region was small. The rate of scye girth varied from 14.3% of M13 to-5.6% of M2. The breadth of a sleeve expanded in all movements, and especially the front region expanded more than the back. 4) The relation between the shoulder length right and under axilla waist length, in all occasions, was contracted mutually, and one contracted, the other expanded. Therefore, in clothing construction when we consider the function of the arm it is better to widen the arm hole and the breadth of a sleeve at the same time than no widen the shoulder length by lowering the upper part of the upper side seam line.

• BMB Reports
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• v.34 no.6
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• pp.573-577
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• 2001

The mammalian heart is known to undergo significant mechanical changes during fetal and neonatal development. The objective of this study was to define the ontogeny of the ryanodine receptor/$Ca^{2+}$ release channel and SERCA that play the major roles in excitation-contraction coupling. Whole ventricular homogenates of fetal (F) (19 and 22 days in gestation), postnatal (N) (1 and 7 days postnatal), and adult (A) (5 weeks postnatal) Sprague-Dawley rat hearts were used to study [$^3H$]ryanodine binding and oxalate-supported $^{45}Ca^{2+}$ uptake. For the ryanodine receptor, the major findings were: (1) The ryanodine receptor density, as determined by maximal [$^3H$]ryanodine binding ($B_{max}$), increased 3 fold between the F22 and A periods ($0.26{\pm}0.1$ vs. $0.73{\pm}0.07$ pmoles/mg protein, p<0.01), whereas there was no significant change during the F22 and N1 development phases ($0.26{\pm}0.1$ vs. $0.34{\pm}0.01$). (2) Affinity of the ryanodine receptor to ryanodine did not significantly change, as suggested by the lack of change in the $K_d$ during the development and maturation. For SERCA, changes started early with an increased rate of $Ca^{2+}$ uptake in the fetal periods (F19: $8.1{\pm}1.1$ vs. F22: $19.3{\pm}2.2$ nmoles/g protein/min; p<0.05) and peaked by 7 days (N7) of the postnatal age ($34.9{\pm}2.1$). Thus, we conclude that the quantitative changes occur in the ryanodine receptor during myocardial development. Also, the maturation of the $Ca^{2+}$ uptake appears to start earlier than that of the $Ca^{2+}$ release.

• IMMUNE NETWORK
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• v.15 no.3
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• pp.135-141
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• 2015

Dysfunction of gut immune regulation is involved in mucosal damage in inflammatory bowel disease (IBD). However, there is still no efficacious immune-regulator for the treatment of IBD. Alloferon is a novel immune-modulatory peptide that was originally isolated from infected insects. It shows anti-inflammatory effects by the regulation of cytokine production by immune cells and their activities. Therefore, we investigated the effect of alloferon in a mouse model of colitis using dextran sulfate sodium (DSS). Colitis was induced by administration of DSS in drinking water for 7 consecutive days. It was confirmed by the presence of weight loss, diarrhea, hematochezia, and colon contraction. Alloferon was injected 4 days after DSS administration. We found that alloferon improved the pathogenesis of IBD based on the reduced disease activity index (DAI) and colon contraction. Edema, epithelial erosion, and immune cell infiltration were found in mice administered DSS, but the phenomena were reduced following alloferon treatment. The plasma level of IL-6, a classical pro-inflammatory cytokine in colitis, was also decreased by alloferon. Moreover, alloferon inhibited the TNF-${\alpha}$-induced degradation and phosphorylation of $I{\kappa}B$ in Colo205 colon cancer cells. Taken together, these results show that alloferon has anti-inflammatory effects and attenuates DSS-induced colitis.

• Journal of the Korea Concrete Institute
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• v.23 no.3
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• pp.289-294
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• 2011

The installation of joint is to prevent random cracking due to drying shrinkage stress of concrete slab. However contraction joint spacing is empirically implemented into slab constructions without detail calculation based on quantitative criteria. In this study, shrinkage strain of concrete due to concrete shrinkage stress was measured to suggest joint spacing based on the study results. The test environmental conditions were applied temperature of $15^{\circ}C$ and relative humidity of 60%. The design compressive strength used was 30 MPa and 40 MPa, which are currently used in concrete slab designs. The drying shrinkage test result was applied to drying shrinkage models (ACI 209R, CEB MC 90, B3, GL 2000 and Sakata). The results showed that the most appropriate model was ACI 209R model. Based on the research findings, quantitative contraction joint spacing locations were calculated.

• YAKHAK HOEJI
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• v.37 no.4
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• pp.397-407
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• 1993

The muscarinic antagonist l-[benzilic-4,4'-$^3H$]quinuclidinyl benzilate([$^3H$]QNB) bound to a single class of muscarinic receptor with high affinity in guinea pig ileal membranes. The $K_{D}$ and B$_{max}$ values for [$^3H$]QNB calculated from analysis of saturation isotherms were 54 pM and 156fmol/mg, respectively. H$_{1}$-blockers inhibited [$^3H$]QNB binding to ileal membranes with $K_{i}$ values ranged from 0.008 $\mu{M}$ to 1.6 $\mu{M}$. The pseudo-Hill coefficients of H$_{1}$-blockers for inhibition of [$^3H$]QNB binding to the ileal membranes were close to unit. The $K_{i}$ values for H$_{1}$-blockers were similar to the $K_{M}$ values calculated by Schild plot of functional data obtained from inhibition of the carbachol-induced contraction in guinea-pig ileum, suggesting that binding of H$_{1}$-blockers vs [$^3H$]QNB in ileal membranes represents an interaction with a receptor of physiological relevance. The $K_{H}$ values of H$_{1}$-blockers for H$_{1}$-receptor estimated from inhibition of the histamine-induced contraction were the range of 0.15 nM to 56.5 nM. The $K_{M}$/K$_{H}$ ratio of H$_{1}$-blockers varied over a wide range of 3 to 2300. Thus, the antihistaminic potencies of H$_{1}$-blockers do not correlate with their antimuscarinic potencies, which suggest that antihistamines have different antimuscarinic potencies in therapeutic blood levels causing similar antiallergic effect. Among 13 traditional antihistaminics examined in this study, drug having the highest and the lowest $K_{M}$/K$_{H}$ ratio is triprolidine and diphenidol, respectively. The present results demonstrate that the antimuscarinic property of antihistamines is not necessary for their antiallergic effect, and data on the affinity of antihistamines for muscarinic and H$_{1}$-receptors can be an important parameter in the selection and evaluation of these drugs.

• Journal of Oral Medicine and Pain
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• v.35 no.1
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• pp.49-59
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• 2010

The purpose of this study was to investigate short-term masticatory muscle reactions in response to simulated noise and music sound. Hypothesis of this study was that loud noise would cause increased stiffness and decreased elasticity of the masticatory muscles compared to low level of noise or identical sound level of music. Fifteen male volunteers were recruited for the study. The sound levels of noise and music used here were 60 dB and 100 dB. The experiment comprised 4 sessions, Session 1 with 100 dB of noise for the 1st day of experiment: Session 2 with 100 dB of music for the $2^{nd}$ day: Session 3 with 60 dB of noise for the $3^{rd}$ day: Session 4 with 60 dB of music for the $4^{th}$ day. Stiffness and elasticity on the anterior temporalis and superficial masseter muscles were measured with tactile sensor before and 2, 4 and 6 minutes after exposure of sound. The study indicated that, in short-term exposure of sound, there was no significant difference between noise and music at both 60 and 100 dB of sound level, but that there were partially significant differences between 60 and 100 dB of sound level regardless of sound type. This suggest that high level of sounds like 100 dB used in this study, in spite of short term exposure of several minutes, would lead to masticatory muscle contraction, especially in the masseter muscles.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.12
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• pp.1716-1723
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• 2003

A stereoscopic PIV(Particle Image Velocimetry) technique was employed to measure the 3 dimensional flow structure of turbulent wake behind a marine propeller with 5 blades. The out-of-plane velocity component was determined using two CCD cameras with the angular displacement configuration. Four hundred instantaneous velocity fields were measured for each of four different blade phases and ensemble averaged to investigate the spatial evolution of the propeller wake in the near-wake region from the trailing edge to one propeller diameter(D) downstream. The phase-averaged velocity fields show the potential wake and the viscous wake developed along the blade surfaces. Tip vortices were generated periodically and the slipstream contraction occurs in the near-wake region. The out-of-plane velocity component and strain rate have large values at the locations of tip and trailing vortices. As the flow goes downstream, the turbulence intensity, the strength of tip vortices and the magnitude of out-of-plane velocity component at trailing vortices are decreased due to viscous dissipation, turbulence diffusion and blade-to-blade interaction.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.3
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• pp.414-426
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• 1997

Numerical computations were conducted to characterize the three-dimensional laminar flow through an injector orifice having an inclined angle of 30 .deg.. For this study, the incompressible Navier-Stokes equations in generalized curvilinear coordinates, using a pseudocompressibility approach for continuity equation, were solved. The computations were performed using the finite difference implicit, approximately factored scheme of Beam and Warming and multi-block grids of complete continuity at block interfaces. The multi-block computations were validated for the steady state using direct comparison of multi-block solutions with equivalent single-block ones, including 2-D 180.deg. TAD and 3-D 90.deg. pipe bend. The comparisons between the numerical solutions and the flow field measurements for a tube with sudden contraction were presented in this work for solution validation. Computational results showed the nature of complex flow fields within the inclined injector orifice, including strong pressure-driven secondary flows in the cross stream induced by the effect of streamline curvature. In addition, asymmetric secondary flows were induced in the Reynolds number range above assumed laminar flow regime considered. However, turbulence calculations and grid dependency studies are needed for more accurate computations.