• Journal of Plant Biology
• /
• 제36권3호
• /
• pp.281-284
• /
• 1993

A karyotype of cytotype BB plant in Scilla scilloides Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two type of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.

• 한국자원식물학회지
• /
• 제35권5호
• /
• pp.659-666
• /
• 2022

이 연구에서는 밀 첨가 계통의 생식세포분열시, 호밀 B 염색체가 염색체 접합에 미치는 영향을 관찰하였다. 밀 첨가 계통은 동조관계에 있는 Leymus mollis와 L. racemosus 염색체를 각각 하나씩 가지고 있다. 밀의 유전적 배경에서 두 Leymus염색체의 이동은 genomic in situ hybridization에 의하여 확인되었다. 호밀 B 염색체를 가지고 있지 않은 밀 첨가 계통의 생식세포 제1중기 분열기에 두 Leymus 염색체의 대부분은 일가염색체를 보였다(98.1 ± 0.5%). 반면에 호밀 B 염색체를 가지고 있는 밀 첨가 계통에서 Leymus 이가염색체의 빈도(10.3 ± 0.2%)는 호밀 B 염색체를 가지고 있지 않은 밀 첨가 계통의 Leymus이가염색체의 빈도(1.9 ± 0.5%)보다 높았다. 호밀 B 염색체를 가지고 있지 않은 밀 첨가 계통에서는 비정상적인 구조를 가지고 있는 이가염색체가 관찰되었다. 반면, 비록 매우 낮은 빈도이지만 호밀 B 염색체를 가지고 있는 밀 첨가 계통에서는 정상적인 형태를 보이는 이가염색체가 확인되었다. 호밀 B 염색체의 영향은 또한 보통밀과 L. racemosus염색체 사이의 이가염색체의 형성을 유도하였으며, 보통밀과 L. mollis 염색체 사이의 삼가염색체의 형성도 유도하였다. 뿐만 아니라 보통밀 염색체 사이에서 초과 교차가 일어나는 것을 확인하였으며, 이러한 현상으로 원, 막대, 그리고 후라이팬 모양의 일반적인 이가염색체의 형태가 아닌 막대모양이나 응축된 형태의 보통밀 이가염색체가 확인되었다.

• Journal of Plant Biology
• /
• 제33권4호
• /
• pp.237-242
• /
• 1990

Geographical distribution of diploid plant with AA genome (2n=16) and allotetraploid with AABB genome (2n=34) of Scilla scilloides Complex from Korea has been studied. The composition and frequencies of B chromosomes ere also investigated. Plants with AABB genome were predominant over AA genome plants. A mixed population of AA and AABB genome plants was found for the first time. Aneuploid plants have not been found. Chromosomes of AA genome were composed of three pairs of metacentric, two pairs of submetacentric, two pairs of subtlocentric and one pair of telocentric chromosomes, whereas BB genome was four pairs of metacentric and five pairs of subtelocentric chromosomes. B chromosomes were classified into two categories, isochromosome (F) and chromosome fragment (f). The frequencies of B chromosomes were 43% in AA genome plants and 44% in AABB genome plants. The number of B chromosome ranged from 1 to 3 and 1 to 7 in AA and AABB genome plants, respectively. B chromosome combinations were F and F+f in AA genome plants and F, F+f and f in AABB genome plants.

• 식물조직배양학회지
• /
• 제21권1호
• /
• pp.59-63
• /
• 1994

세포유전적 유형이 다른 무릇 (Scilla scilloidise Coplex)의 조직배양에서 유도된 캘러스에서 염색체 변이를 조사하였다. AA유형의 캘러스에서 심한 수적, 구조적 변이가 관찰된 반면, BB 유형의 캘러스 세포에서는 상염색체의 변이가 발견되지 않았다. 이질 4배체인 AABB유형에서는 두개의 hypoploid 세포만 관찰되었으나, B게놈의 진정4배체인 BBBB유형에서는 hypoploid 세포와 함께 hyperploid 세포가 관찰되었다. 캘러스 세포에서 상염색체의 안정성은 B염색체를 지닌 식물에서 유도된 캘러스에서 더 높게 나타났다. 이는 배양세포에서 B염색체가 상염색체의 안정성에 선택적으로 기능하기 때문인 것으로 여겨진다.

• 한국미생물·생명공학회지
• /
• 제23권5호
• /
• pp.544-549
• /
• 1995

The alkaline elastase is an extracellular serine protease of the alkalophilic Bacillus strain Ya-B. To increase the gene copy number and the production level of the alkaline elastase Ya-B, we designed, on the B. subtilis chromosome, a gene amplification of the 10.6 kb repeating unit containing amyE, aleE (alkaline elastase Ya-B gene) and tmrB. The aleE was inserted between amyE and tmrB, and B. subtilis APT119 strain was transformed with this amyE-aleE-tmrB-junction region fragment. As a result, we succeeded in obtaining tunicamycin-resistant (Tm$^{r}$) transformants (Tf-1, Tf-2) in which the designed gene amplification of 10.6 kb occurred in chromosome. The transformants showed high productivity of $\alpha $-amylase and alkaline elastase Ya-B. The copy number of the repeating unit (amyE-aleE-tmrB) was estimated to be 25, but plasmid vector (pUC19) was not integrated. The amplified aleE of chromosome was more stable than that of plasmid in absence of antibiotics.

• 한국자원식물학회지
• /
• 제4권1호
• /
• pp.5-12
• /
• 1991

A combination of acerocarininc-Wright C-banding technique was utilized to identify each chromosomes in durum wheat ,Triticum durum var. Hordeiforme (2n=4x=28 AABB), This technique elucidated qualitativr and quantitative traits of the indi-vidual chromosomes In coinplement. Most comspicuous bands were observed at thecentromere of B-genome chronmosomes. Each chromosomes of A-genome had some-what weak centromeric, proximal and terminal bands. Chromosomes 2A and 4A hasa small subterminal bands. 6A is smallest and metacentric chromosome and , has two faint interstitial band. Chromosomes 1B and 6B showed satellite and constriction lage band. Short arm of 3B has three heavily interstitial bands. Both arms of chromosome 4B has a lagc centromeric band and a very lage proximal band. 5B had heavilycentromeric band and the long arm showed prominent two interstitial bands. Chromo-somes 25 and 7B has a small terminal band of both arms.

• 미생물학회지
• /
• 제25권2호
• /
• pp.103-109
• /
• 1987

An ozone-sensitive mutant of Escherichia coli strain B, MQ 1844 is described. Its properties, including high sensitivity to ozone and radiation, inducible filamentation, extensive DNA degradation and impaired DNA synthesis following ozonation, are attributable to a mutation in ozrB, a gene which is cotransducible with malB. Based on differences in phenotypic expression as well as on the particular location of this gene on the bacterial chromosome, ozrB appears as distinct from the other ozone-or radiation-sensitivity genes previously described.

• Journal of Plant Biology
• /
• 제14권4호
• /
• pp.14-18
• /
• 1971

Lilium Miquelianum Makino is a species which originated in Korea. The Karyotype of the species was examined in materials collected at Mts. Kaya, Kasan, Chejung, and Kaji. The results are as follows: 1) The somatic chromosome number was found to be 2n=24. 2) The karyotype is described as: K=2Am＋2Bkm＋2Csst＋2Dsst＋2Ests＋2Fst＋2Gst＋2Hst＋2Ist＋2Jsst＋2Kst＋2Lst m: metacentric, sm: submetacentric st: subtelocentric, s: secondary constriction 3) A single subcentric supernumerary B-chromosome was found in some bulbs from Mt. Kasan. 4) The shape of the supernumerary B-chromosome was similar to that of the E chromosome which had separated at its secondary constriction and lost its lower chromosome fragment. 5) From three to eight nucleio of varying sizes were found in the telophase or interphase nucleus of root tip cells. The maximum number of eight nucleoli corresponds to the number of chromosomes that have a secondary constriction.

• BMB Reports
• /
• 제29권3호
• /
• pp.241-247
• /
• 1996

The Philadelphia (Ph) chromosome is the single most intensively studied chromosome alteration characterizing a human malignancy. The specific genetic alteration of chronic myelogenous leukemia (CML) is the formation of the BCR/abl fusion gene in leukemic cells. The presence of the BCR/abl gene has important diagnostic and prognostic implications in CML. The detection of BCR/abl transcripts by reverse transcriptase based polymerase chain reaction (RT-PCR) was investigated in patients with CML in whom the Ph chromosome abnormality was documented by cytogenetic analysis. In a total of 68 CML patient cases, the Ph chromosome was found in 53 cases (77.9%) by cytogenetic analysis. On the other hand, sixty two cases (91.2%) were detected to have BCR/abl gene rearrangement Of these, b3a2 was 44 cases (64.7%) and b2a2 was 17 cases (25,0%). There was one case with both b3a2 and b2a2 (1.5%). Of the fifteen cases of Ph chromosome negative by cytogenetic anlaysis, the BCR/abl gene was observed in nine cases, The results of BCR/abl fusion gene confirmed by the direct sequencing method correlated well with PCR analysis, The amplified PCR products were detected by $1{\times}10^{-5}$ dilutions. In conclusion, PCR technique is sensitive, rapid and relatively simple for a laboratory test in detecting the BCR/abl fusion gene with CML regardless of the result of cytogenetic analysis.

• Genomics & Informatics
• /
• 제17권3호
• /
• pp.27.1-27.9
• /
• 2019

Supernumerary B chromosomes were found in Lilium amabile (2n = 2x = 24), an endemic Korean lily that grows in the wild throughout the Korean Peninsula. The extra B chromosomes do not affect the host-plant morphology; therefore, whole transcriptome analysis was performed in 0B and 1B plants to identify differentially expressed genes. A total of 154,810 transcripts were obtained from over 10 Gbp data by de novo assembly. By mapping the raw reads to the de novo transcripts, we identified 7,852 differentially expressed genes (log₂FC > |10|), in which 4,059 and 3,794 were up-and down-regulated, respectively, in 1B plants compared to 0B plants. Functional enrichment analysis revealed that various differentially expressed genes were involved in cellular processes including the cell cycle, chromosome breakage and repair, and microtubule formation; all of which may be related to the occurrence and maintenance of B chromosomes. Our data provide insight into transcriptomic changes and evolution of plant B chromosomes and deliver an informative database for future study of B chromosome transcriptomes in the Korean lily.