• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.958-963
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• 2008

A series of experiments were conducted to assess the effectiveness of rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase for growth promotion of peas under drought conditions. Ten rhizobacteria isolated from the rhizosphere of different crops (peas, wheat, and maize) were screened for their growth promoting ability in peas under axenic condition. Three rhizobacterial isolates, Pseudomonas fluorescens biotype G (ACC-5), P. fluorescens (ACC-14), and P. putida biotype A (Q-7), were selected for pot trial on the basis of their source, ACC deaminase activity, root colonization, and growth promoting activity under axenic conditions. Inoculated and uninoculated (control) seeds of pea cultivar 2000 were sown in pots (4 seeds/pot) at different soil moisture levels (25, 50, 75, and 100% of field capacity). Results revealed that decreasing the soil moisture levels from 100 to 25% of field capacity significantly decreased the growth of peas. However, inoculation of peas with rhizobacteria containing ACC deaminase significantly decreased the "drought stress imposed effects" on growth of peas, although with variable efficacy at different moisture levels. At the lowest soil moisture level (25% field capacity), rhizobacterial isolate Pseudomonas fluorescens biotype G (ACC-5) was found to be more promising compared with the other isolates, as it caused maximum increases in fresh weight, dry weight, root length, shoot length, number of leaves per plant, and water use efficiency on fresh and dry weight basis (45, 150, 92, 45, 140, 46, and 147%, respectively) compared with respective uninoculated controls. It is highly likely that rhizobacteria containing ACC deaminase might have decreased the drought-stress induced ethylene in inoculated plants, which resulted in better growth of plants even at low moisture levels. Therefore, inoculation with rhizobacteria containing ACC deaminase could be helpful in eliminating the inhibitory effects of drought stress on the growth of peas.

• 농약과학회지
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• 제4권1호
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• pp.51-58
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• 2000

본 연구는 담배가루이(Bemisia tabaci, B. biotype)의 방제약제를 선발하는 목적으로 시판되고 있는 43종의 살충제에 대한 발육단계별 감수성을 조사하고, 침투이행성, 잔효성 및 방제효과를 평가하였다. 모든 시험은 살충제의 추천농도(ppm)로 수행하였다. 알에 대해서는 IGR계인 pyriproxyfen과 kiflubenzuron이 95%이상의 부화억제효과를 나타내었다. 3령약충에 대해서는 abamectin, acetamiprid, imidacloprid, pyriproxyfen, acetamiprid+ethofenprox이 95%이상의 살충효과를 나타내었다. 그리고 성충에 대해서는 유기인계의 diazinon, fenitrothion, methidathion, pirimiphos-methyl, neonicotinoid계의 acetamiprid, imidacloprid, 혼합제의 acetamiprid+ethofenprox, cartap hydrochloride+buprofezin, fenpropathrin+fenitrothion 기타 abamectin, endosulfan, pymetrozine, spinosad 등이 높은 살충율을 나타내었다. Abamectin, acetamiprid, imidacloprid, pyriproxyfen, acetamiprid+ethofenprox은 성충에 대해서 잔효 성뿐만아니라 침투이행성효과도 나타내었다. 방제효과시험에서 abamectin, acetamiprid, imidacloprid, acetamiprid+ethofenprox 은 처리후 1일째, pyriproxyfen은 처리후 7일째부터 90%이상의 방제효과를 나타내었다. 이상의 결과에서, abamectin, acetamiprid, imidacloprid, pyriproxyfen, acetamiprid+ethofenprox은 담배가루이의 방제에 이용될 수 있을 것으로 생각된다.

• 대한수의학회지
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• 제27권2호
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• pp.227-233
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• 1987

This study was conducted to determine the epidemiological characteristics of Campylobacter enteritis. A total of 187 fecal specimens of Korean native goat were examined for the presence of C. jejuni and C. coli by direct plating. Fifty strains isolated were examined for biochemical and serological properties and susceptibility to 19 chemotherapeutic agents. A total of 29(15.5%) C. jejuni and 21 (11.2%) C. coli were isolated from the fecal specimen of 187 Korean native goats. Of the 50 isolates of C. jejuni and C. coli, 29 isolates of C. jejuni grouped as 7 biotypes (1,2,3,4,6,7 and 8) and biotypes 1(34.5%), 2(17.2%) and 3(20.7%) were encountered most frequently. Twenty-one C. coli strains were differentated into biotype I (61.9% of the isolates) and biotype II (38.1%). Of the 29 C. jejuni strains examined, 24(83.0%) were typable by the Lior serotyping scheme and five isolates were non typable. C. jejuni grouped as 8 serotypes, serotype 4(24.1%) and 26(20.7%) were encountered most frequently. In the case of 21 strains of C. coli grouped as 6 serotypes, the most frequent serotypes were 21(28.6%) and 25(23.8%). Total of 50 strains of isolated were all susceptible to amikacin, clindamycin and tobramycine. Overall 85% of isolates were sensitive to erythromycin, doxycycline, chloramphenicol, flume-quine, kanamycin, gentamicin, nalidixic acid, polymyxin B, colistin, tetracycline and ampicillin, but about 65% of isolates were resistant to cefamandole and ethyl hydrocuprein hydrochloride.

• 대한수의학회지
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• 제27권2호
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• pp.235-243
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• 1987

This paper deals with the distribution of Yersinia spp. isolated from the feces or the cecal contents of 1,755 pigs, 558 cows, 428 pigs slaughtered, 271 dogs slaughtered and 91 deer during the period of March 1985 to February 1986. Isolated Yersinia spp. were examined for serotype, biotype and antibiotic susceptibility of Y. enterocolitica. The results were as follows; One hundred and fourty-three stains of Yersinia spp. were isolated from 141(4.5%) out of 3,103 animals examined and their isolates were identified as Y. enterocolitica(138 strains), Y. kristensenii (3 strains), Y. intermedia(1 strain) and Y. pseudotuberculosis(1 strain). Yersinia spp. were isolated from 122(7.0%) of 1,755 pigs in piggeries, 15(3.5%) of 428 pigs slaughtered and 4(1.5%) of 271 dogs slaughtered, but no Yersinia spp. were isolated from cows and deer. The isolation rate of Yersinia spp. in pigs ranged from 5.9~8.0% in piggeries, it was higher in summer and autumn and highest in fattening pigs groups(10.4%), especially. One hundred and thirty-eight Y. enterocolitica isolates belonged to serotype 0 : 3(95 strains), 0 : 8(13 strains), 0 : 5(7 strains), 0 : 9(6 strains), 0 : 1, 2(1 strain) and untypable(16 strains), among them strains of serotype 0 : 3 biotype 3B(91 strains) were predominant. Antibiotic susceptibility test of 138 isolates of Yersinia spp. was performed by the agar dilution method, using 8 antibiotics as follows: ampicillin(Am), chloramphenicol, kanamycin, nalidixic acid(Na), rifampicin(Rf), streptomycin, sulfadimethoxine(Su) and tetracycline. All the strains tested were susceptible to Rf and Na, but resistant to Su, and 136 strains(98.6%) were also resistant to Am.

• 한국임상수의학회지
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• 제40권4호
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• pp.308-313
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• 2023

A 1-year-old castrated male Korean Shorthair cat presented with dyspnea, anorexia, lethargy, and seizures. Physical examination revealed salivation, right forelimb hemiparesis, and rapid breathing. No abnormalities were detected on auscultation. Laboratory findings revealed increased levels of bilirubin, aspartate aminotransferase (AST), globulin, glucose, and a decreased albumin-to-globulin (A:G) ratio. Both N-terminal pro-B-type natriuretic peptide (NT-proBNP) and feline serum amyloid A (fSAA) levels were significantly elevated. Thoracic radiography revealed mild cardiomegaly and diffuse increased interstitial infiltration with soft tissue opacity in the periphery of the right caudal pleural space. Echocardiography and lung ultrasonography were performed to investigate the cause of mild cardiomegaly and soft tissue opacity in the pleural space. Echocardiography revealed a mild amount of echogenic pericardial effusion, and lung ultrasonography showed an echogenic soft tissue mass with no blood signal in the right caudal pleural space, suggestive of a granulomatous lesion. After obtaining 5 mL of pericardial fluid through pericardiocentesis, cytology of the pericardial effusion sample revealed marked neutrophils and macrophages with no bacteria. IDEXX feline infectious peritonitis (FIP) virus real-time reverse transcriptase polymerase chain reaction (RT-PCR) confirmed the presence of the FIP virus biotype in the sample. This case presents a rarely reported atypical mixed form of FIP in a cat diagnosed ante-mortem using pericardial effusion analysis. In this case, ultrasound examination played a crucial role in the definitive diagnosis of FIP by PCR biotyping through pericardiocentesis. Ultrasonography can be highly beneficial in guiding the diagnosis and evaluation of cats with suspected FIP.

• 농약과학회지
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• 제7권3호
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• pp.207-215
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• 2003

본 연구에서는 3종 (acetamiprid, acetamiprid+etofenprox, etofenprox)의 살충제에 대한 온실가루이(Trialeurades vaparariarum)와 담배가루이 (Bemisia tabaci, B biotype)의 발육단계별 감수성을 조사하고, 침투이행성, 잔효성 및 방제효과를 평가하였다. 시험은 살충제의 추천농도를 비롯한 2 가지 농도 (ppm)로 수행하였다. 알에 대해서는 acetamiprid 단제 40ppm 처리시 온실가루이와 담배가루이에서 각각 45%, 42%의 부화억제효과를 나타내었으며, 3령 약충에 대해서는 acetamiprid 20 ppm 농도에서도 각각 97% 이상의 살충효과를 나타내었다. 그리고, 성충의 경우 acetamiprid 20 ppm 과 acetamiprid + etofenprox(12.5+40) ppm 농도에서도 92% 이상의 우수한 방제효과를 나타내었다. acetamiprid 와 acetamiprid + etofenprox는 성충에 대해서 잔효성뿐만 아니라 침투이행 효과도 나타내었다. 방제효과시험에서 acetamiprid와 acetamiprid+etofenprox를 추천농도로 처리시에 처리후 3일째부터 90% 이상의 방제효과를 나타내었다. 이상의 결과에서 acetamiprid 와 acetamiprid + etofenprox는 온실가루이와 담배가루이 성충과 약충의 방제에 효과적으로 이용될 수 있을 것으로 생각된다.

• 한국토양비료학회지
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• 제23권2호
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• pp.152-159
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• 1990

밀의 근권(根圈)에서 병원성(病原性) 사상균(絲狀菌)에 대해 길항성(拮抗性)이 높은 균주(菌株)를 분리(分離), 동정(同定)하여 연작장해(連作障害)의 피해경감과 생물방제(生物防除)의 기초자료(基礎資料)를 삼고자 그 균이 생성(生成)하는 siderophore에 의한 사상균(絲狀菌)의 생육(生育) 저해력(沮害力)을 조사한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 밀의 근권(根圈)에서 형광성(螢光性) 세균(細菌)을 선별하여 R. solani, F. oxysperum, F. solani에 대한 생육저해(生育沮害)가 큰 (10mm이상) 균주(菌株)를 P. fluerescens Biovar I(Ps-1, Ps-2, Ps-5)과 Biovar II(Ps-3)로 동정(同定)되었다. 2. 선발균주(選拔菌株)가 생성(生成)하는 색소(色素)는 green-yellow 로 410nm에서 최대의 흡광도(吸光度)를 나타내었다. 3. Siderophore는 $Fe^{+3}$의 첨가시 생성량(生成量)은 현저히 감소하였으나 $Fe^{+2}$의 첨가시에는 생성량(生成量)의 감소는 보이지 않았다. 4. 선발균주(選拔菌株)중 Ps-5가 생성(生成)한 색소(色素)는 R. solani의 생육(生育)에 영향을 미치지 않았으며 $FeSO_4$의 처리시 생육(生育)이 증가되었다. 그러나 균주(菌株) Ps-1, Ps-2, Ps-3가 생성(生成)한 색소(色素)는 R. solani, F. oxysperum, F. solani에 대한 생육저해(生育沮害)는 컷으며 $Fe^{+2}$의 첨가시 저해력(沮害力)은 현저히 감소되었다. 5. 암소재배법(暗所栽培法)을 이용한 오이의 연작지토양(連作地土壤)에 오이를 파종하여 접종효과(接種效果)를 본 결과(結果) Ps-2>Ps-1>Ps-5>Ps-3의 순(順)으로 접종효과(接種效果)가 있었으며 Ps-1과 Ps-2는 captan 50ppm의 처리구 보다도 효과(效果)가 더 좋았다.

• 한국작물학회지
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• 제57권4호
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• pp.365-372
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• 2012

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.

• 한국응용곤충학회지
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• 제40권3호
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• pp.203-209
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• 2001

담배가루이(B biotype)의 온도조건과 기주별로 발육과 생식에 미치는 영향을 조사하였다. 알에서 성충까지의 발육기간은 $15^{\circ}C$에서 86.2일이었고, $30^{\circ}C$에서는 17.0일로 온도가 높아질수록 약 5배 짧았다. 알, 약충 그리고 알에서 성충까지의 발육영점온도는 각각 $10.1^{\circ}C$, $11.6^{\circ}C$, $11.1^{\circ}C$였고, 유효적산온도는 각각 110.3, 204.7, 317.3일도였다. 부화율은 $25^{\circ}C$에서 87.0%, 우화율(부화유충에서 성충까지)은 $20^{\circ}C$에서 76.7%로 높았다. 성충수명은 $20^{\circ}C$에서 23.6일이었고, $30^{\circ}C$에서는 14.0일로 온도가 높을수록 짧았다. 그리고 암컷한마리당 평균 총산란수는 $25.0^{\circ}C$에서 103.3개로 가장 많았으나 온도간에 유의성은 없었다. 1세대당 순증식율($R_{o}$)은 $25^{\circ}C$에서 97.33로 가장 높았다. 그리고 내적자연증가율($r_{m}$)은 온도가 높아 질수록 높아졌으며, $30.0^{\circ}C$에서 0.196으로 가장 높았다. 토마토, 고추, 가지, 포인세치아를 기주로 $25^{\circ}C$ 조건에서 알에서 우화까지의 발육기간은 각각 21.2, 28.1, 22.2, 25.5일이었다. 부화율은 고추에서 90.3%로 가장 높았고, 우화율은 가지에서 89.6%로 가장 높았다. 성충수명은 가지에서 26.5일로 가장 길었고, 고추에서 가장 짧았다. 암컷 한마리당 평균 산란수는 토마토와 가지에서 많았다. 1세대당 순증식율($R_{o}$)은 가지에서 106.1로 가장 높았고, 내적자연증가율($r_{m}$)은 토마토에서 0.165로 가장 높았다. 이상의 결과로 담배가루이의 성장에 적합한 온도범위는 $25~30^{\circ}C$이었고, 가장 적합한 기주식물은 토마토와 가지로 분석되었다.

• 대한수의학회지
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• 제28권1호
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• pp.67-73
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• 1988

This study was conducted to isolate etiological agents from the 103 scouring piglets in Gyeongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical served. The incidence of scouring piglet was most prevalent as 81.6% in the age of 2 to 4 weeks after birth, while the rate was less than 10% in the age of 5 to 6 weeks and under 1 week after birch. When compared the isolation frequency of the each etiological agent, enteropathogenic E. coli was most prevalent as 46.6%, thermophilic Campylobacter 26.2% and Salmonellae was 8.7% in order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85 (20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni were belong to type I (50.0%) and II (25.0%), and most strains of C. coli were belong to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C. and each 1 strain was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, wherease about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C.jejuni and C. coli were sensitive to kanamycin and gentamicin.