• Proceedings of the Safety Management and Science Conference
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• 2002.11a
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• pp.239-246
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• 2002

In this report, we provide the focus on suggesting a method of estimating & measurement of CBM(Customer Behavior Model). Through the use of internet, a new trend of business for e-CRM on B2C Web Site which is now known as EC has emerged. The Purpose of this study is to identify the relationship between the customers of a shopping mall and CBM characteristics. Result showed that there is a significant relationship between the some customers pattern of shopping mall and CBM, CVM(Customer Visit Model).

• Journal of Life Science
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• v.20 no.2
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• pp.260-268
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• 2010

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Channa argus were purified and characterized by biochemical, immunochemical and kinetic methods. The activity of LDH in skeletal muscle was the highest at 380.4 units and those in heart, eye and brain tissues were 13.4, 3,5 and 5.4 units, respectively. Citrate synthase (EC 4.1.3.7, CS) activity in heart tissue was the highest at 20.7 units. LDH/CS in skeletal muscle, heart, eye and brain tissues were 172.9, 0.6, 0.32 and 0.47. Protein concentration in skeletal muscle tissue was 14.7 mg/g and specific activities of LDH in skeletal muscle, heart, eye and brain tissues were 25.88, 0.79, 0.31 and 1.38 units/mg, respectively. Therefore, skeletal muscle tissue was anaerobic and heart tissue was aerobic. The LDH isozymes in tissues were identified by polyacrylamide gel electrophoresis, immunoprecipitation and Western blot with antiserum against $A_4$, $B_4$, and eye-specific $C_4$. LDH $A_4$, $A_3B$, $A_2B_2$. $AB_3$ and $B_4$ isozymes were detected in every tissue, $C_4$, $AC_3$, $A_2C_2$ and $A_3C$ were detected in eye tissue, and $A_3C$ was found in brain tissue. LDH $A_4$, $A_3B$, $A_2B_2$, $AB_3$, $B_4$, eye-specific $C_4$ isozymes were purified by affinity chromatography and Preparative PAGE Cells. The LDH $A_4$ isozyme was purified in the fraction from elution with $NAD^+$ containing buffer of affinity chromatography. Eye-specific $C_4$ isozyme was eluted right after $A_4$, after which $B_4$ isozyme was eluted with plain buffer. As a result, one part of molecular structures in $A_4$, $B_4$ and eye-specific $C_4$ were similar, but were different from each other in $B_4$ and $C_4$. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The activity of LDH $A_4$, $A_2B_2$, $B_4$, and eye-specific $C_4$ isozymes remained at 39.98, 21.28, 19.67 and 16.87% as a result of the inhibition by 10 mM of pyruvate, so the degree of inhibition was very high. The $Km^{PYR}$ values were 0.17, 0.27 and 0.133 mM in $A_4$, $B_4$ and eye-specific $C_4$ isozymes, respectively. The optimum pH of LDH $A_4$, $B_4$, eye-specific $C_4$, $A_2B_2$, $A_3B$, and $AB_3$ were pH 6.5, pH 8.5, pH 5.5, pH 6.0-6.5, pH 5.0 and pH 7.5. The $A_4$ and heterotetramer isozymes stabilized a broad range of pH. Especially, LDH activities in skeletal muscle tissue were high, resulting in a high degree of muscle activity.LDH metabolism in eye tissue seems to be converted faster from pyruvate to lactate by eye-specific $C_4$ isozyme as eye-specific $C_4$ have the highest affinity for pyruvate, and right after the conversion, oxidation of lactate was induced by $A_4$ isozyme. It was found that expression of Ldh-C, affinity to substrate and reaction time of $C_4$ isozyme were different according to the ecological environmental and feeding capturing patterns.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.490-495
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• 1990

The extracellular inulinase from Bacillus spp. was purified to a single protein through a sequence of operations including ammonium sulfate fractionation, heat treatment, DEAE Sepharose C1-6B ion exchange chromatography, Sephadex 6-100 and Sephadex 6-150 gel filtration. The purified enzyme was confirmed to be a $\beta$ -D-fructofuranosidase(EC 3.2.1.26) which was much more active on sucrose than on inulin(I/S = 0.2). The maximal inulinase activity was observed at pH 6.0 and at the temperature of $50^{\circ}C$. The mo1ecular weight of the enzyme was about 56, 000. Tryptophan and histidine residues of the enzyme molecule were found to be essential for its catalytic activity.

• Management & Information Systems Review
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• v.17
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• pp.243-261
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• 2005

A research model based on the concept of a strategic grid was developed to examine the relationship between the role of information system(IS) and organizational use of the web-based electronic commerce(EC) system to achieve business competitiveness. A survey questionnair was sent eletronically to 141 internet shopping malls which sell physical goods direct to an individual end consumer and 125 usable responses were abtained. The results indicated that firms with the strategic views of IS make more use of web-based EC system than firms with support views of IS to achieve their bossiness goals.

• Research in Plant Disease
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• v.12 no.1
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• pp.32-39
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• 2006

This study has been conducted to investigate the responses of various isolates of four Colletotrichum species such as C. gloeosporioides, C acutatum, C. coccodes, and C. dematium isolated from infected tissues of several crops to fungicides such as carbendazim, carbendazim+diethofencarb, four protective fungicides, and three ergosterol biosynthesis-inhibiting (EBI) fungicides. All the isolates of C. acutatum showed $EC_{50}$ values in a range of 0.001-3.040 ${\mu}g/ml$ against carbandazim, a benzimidazole fungicide. As for the response to carbendazim, the isolates of C. gloeosporioides obtained from pepper, apple, and strawberry were clearly divided into two groups, resistant or sensitive isolates. All the resistant isolates showed $EC_{50}$ values above 1000 ${\mu}g/ml$, whereas the sensitive isolates had lower $EC_{50}$ values than 0.550 ${\mu}g/ml$. The isolates of C. gloeosporioides exhibited a negative cross resistance between carbendazim and diethofencarb (a N-phenylcarbamate fungicide), but isolates of C. acutatum did not. Toward carbendazim, C. coccodes and C. dematium isolates showed a similar response to C. acutatum isolates and the sensitive isolates of C. gioeosporioides, respectively. As for response of Protective fungicides, all the isolates of C. acutatum showed a more resistant reaction than all the isolates of C. gloeosporioides. However, there was no difference among 4 species of Colletotrichum against EBI fungicides.

• Journal of Life Science
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• v.14 no.4
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• pp.708-715
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• 2004

The lactate dehydrogenase (EC 1.1.1.27, LDH) in liver of Lempetra japonica was purified in buffer of affinity chromatography. The liver-specific $C_4$ isozyme of Gadus macrocephalus was purified by heat treatment, affinity chromatography, and DEAE-Sephacel chromatography. The liver-specific $C_4$ isozyme was eluted in a buffer containing NAD+ and was coeluted with $B_4$isozyme in plain buffer of affinity chromagraphy. Liver-specific $C_4$ isozyme in G. macrocephalus was the most thermostable, and$B_4$isozyme was more stable than $A_4$. The LDH in the fraction of pH 7.45 purified from the liver of L. iaponica by chromatofocusing was more inhibited by pyruvate than purified LDH. The optimum pH of the LDH isozyme in the liver of L. japonica was 7.5 and that of liver-specific$C_4$ isozyme was 8.5. The LDH in liver of L. japonica made complexes more with antibody against Coreoperca herzi$A_4$ and liver-specific $C_4$ than with that against eye-specific $C_4$. Therefore, the structure of the LDH in liver of L. japonica might be similarly evolved to that of subunit A and liver-specific $C_4$ isozyme in liver tissue of G. macrocephalus. The evolution rate of subunit C is faster than that of subunit A. LDH in liver of L. japonica has not one isozyme but isozymes and it was also found out to have not only subunit A and B but also subunit C.

• Korean Journal of Agricultural Science
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• v.37 no.2
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• pp.191-197
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• 2010

The objective of this research was to determine the influence of the physical and chemical properties of root substrates used during the production of 'Maehyang' strawberry propaguleson the growth of the mother plants and the rate of daughter plant formation. Plants were cultured in plastic bags containing six different formulations of root substrates composed of: a) 50% coir dust and 50% perlite (5:5 by volume, A), b) 60% coir dust and 40% perlite (6:4, B), c) 70% coir dust and 30% perlite (7:3, C), d) 70% coir dust and 30% coconut chip (7:3 D), e) 60% coir dust and 40% coconut chip (60:40, E), or f) 50% sphagnum peat and 50% vermiculite (50:50, F). All media formulations contained a moderate level of base fertilizers. Physical and chemical properties of each formulation were determined before plant establishment and after 120 days of stock plant culture and runner production. Total porosity (TP) and container capacity (CC) of all substrate formulations were higher than 85% and 55%, respectively, allowing a suitable range of air and water holding characteristics. Formulation F provided the highest TP and CC values among the all substrate modifications evaluated. Substrate formulations A, B, C and F had higher electrical conductivity (EC) and $NO_3{^-}$-N concentrations than formulations D and E, when determined before and after plant culture. Formulations A, B, C, and F, having higher EC readings, also performed better as root substrates thanthe formulations D and E in increasing fresh and dry weights of the runners as well as the production of daughter plants per plant. The 'Maehyang' strawberry plants grown in the formulation F had the highest tissue N content, followed by those grown in substrate B, A, C, or D for 120 days after transplanting. Formulation F also facilitated accumulation of higher tissue phosphorus (P) and copper (Cu) contents compared to other treatments. Results of this experiment suggest that the chemical properties, rather than physical properties, of root substrates had a major influence on the growth of mother plants and the occurrence of healthy daughter plants during the bag-culture phase of propagation.

• Earthquakes and Structures
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• v.6 no.4
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• pp.351-373
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• 2014

This paper investigates the seismic response of lightweight acceleration-sensitive non-structural components (NSCs) mounted on irregular reinforced concrete (RC) primary structures (P-structures) using non-linear dynamic finite element (FE) analysis. The aim of this paper is to study the influence of NSC to P-structure vibration period ratio, peak ground acceleration, NSC to P-structure height ratio, and P-structure torsional behaviour on the seismic response of the NSCs. Representative constitutive models were used to simulate the behaviour of the RC P-structures. The NSCs were modelled as vertical cantilevers fixed at their bases with masses on the free ends and varying lengths so as to match the frequencies of the P-structures. Full dynamic interaction is considered between the NSCs and P-structures. A set of 21 natural and artificial earthquake records were used to evaluate the seismic response of the NSCs. The numerical results indicate that the behaviour of the NSCs is significantly influenced by the investigated parameters. Comparison between the FE results and Eurocode (EC8) predictions suggests that EC8 underestimates the response of NSCs mounted on the flexible sides of irregular RC P-structures when the fundamental periods and heights of the NSCs match those of the P-structures. The perceived cause of this discrepancy is that EC8 does not take into account the amplification in the dynamic response of NSCs induced by the torsional behaviour of RC P-structures.

• Proceedings of the CALSEC Conference
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• 2001.08a
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• pp.493-501
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• 2001

B2C is not doing well in Asia and Pacific countries in comparison to U.S. and Europe. But there is misunderstanding of low growth of B2C electric commerce caused by cultural factor. Consumers in market are rational to decide their purchase according to total cost - market price and transaction cost. I examined this with data of Korea and U.S. Transaction cost in real market is less in Korea than in U.S. but that of electric commerce, it is much less in U.S. As a result, the choice of consumers in Korea is real market transaction while consumers in U.S. choose electric commerce.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.spc
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• pp.45-46
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• 2009

This study was carried out to identify the changes of EC during desalinization due to flooding in newly reclaimed saline soil. To do this, experimental plots were made of rotary tillage+water exchanging plot, flooding plot and rainfall flooding plot. In rotary tillage+water exchanging plot, drainage, rotary tillage and flooding were conducted at the interval of 7 days. In rotary tillage+water exchanging plot and flooding plot, plots were irrigated at the height of 10 cm. After 38 days desalinization, changes of EC values at top soil (0~20 cm) were as follows. In rotary tillage+water exchanging plot, EC decreased from $21.38dS\;m^{-1}$ to $2.16dS\;m^{-1}$ and in flooding plot, EC decreased from $13.97dS\;m^{-1}$ to $2.22dS\;m^{-1}$. In rotary tillage+water exchanging plot and flooding plot, EC values decreased below the EC criterion ($4.0dS\;m^{-1}$) of saline soil. In rainfall flooding plot, EC values decreased or increased according to amounts of rainfall and rainfall time. After 38 days, EC decreased from $16.7dS\;m^{-1}$ to $12.35dS\;m^{-1}$. In flooding plot, changes of EC due to soil depth were investigated. After 38 days desalinization, changes of EC due to soil depth were as follows. At 0~10 cm depth, EC value decreased from $13.08dS\;m^{-1}$ to $0.74dS\;m^{-1}$ (94.3% of salt was desalinized). At 10~20 cm depth, EC value decreased from $14.80dS\;m^{-1}$ to $3.69dS\;m^{-1}$ (75.2% of salt was desalinized). At 20~30 cm depth, soil was desalinized slowly compared with upper soil, EC value decreased from $13.57dS\;m^{-1}$ to $6.93dS\;m^{-1}$ (48.9% of salt was desalinized).