• Journal of Sericultural and Entomological Science
• /
• v.27 no.2
• /
• pp.7-19
• /
• 1985

The genetic variances and combinding ability of some useful characters were analyzd on sixty four combinations in the 8$\times$8 diallel cross using the four Japanese races(Jam 107, Jam 113, Jam 117 and Jam 119) and the four Chinese races(Jam 108, Jam 114, Jam 118 and Jam 120). The eight quantitative characters were the total larval stage period(TP), the fifth larval instar period(FP), the female cocoon total weight(FW), the male cocoon total weight(MW), the female cocoon layer weight(FL), the male cocoon layer weight(ML), the female cocoon layer ratio(FR), and the male cocoon layer ratio(MR). The results were as follows: The analysis of the genetic variance and the combining ability in the TP and the FP. In TP and FP, h2N was less than h2B. The GCA, SCA and RCA were at a high significant level. Hl/D and (Hl/D)1/2 ere large. The heterosis were small minus. E and D were large. The r was in the positive direction, because the recessive genes were mainly expressed as a short rearing periods. The regressions of the characters were passed below 0 point, because the characters in the TP and the FP were appeared overdominant. The order of the dominance in the TP of the parents were in the order of Jam 119>Jam 113>Jam 117>Jam 108>Jam 120>Jam 114>Jam 107>Jam 118, and that in the FP of the parents were followed in the orders of Jam 117>Jam 113>Jam 108>Jam 114>Jam 119>Jam 107>Jam 120>Jam 118. The analysis of the genetic variance and the combining ability of the FW and the MW. In the FW and the Mw, h2N was less the h2B. The GCA and SCA were large but RCA was little. Hl/D and (Hl/D)1/2 in the parents were large. Heterosis was large. E was appeared large in the FW, and small in the MW. D was small. The r was of the minus direction, because the dominance genes were less expressed. The regression of the these characters were padded below 0 point, because the characters in FW and MW were appeared overdominant. The orders of the dominance in the FW of the parents were as the order of Jam 107>Jam 108>Jam 119>Jam 113>Jam 114>Jam 120>Jam 117>Jam 118, and in the MW of them in the order of Jam 114>Jam 120>Jam 108>Jam 113>Jam 107>Jam 119>Jam 117>Jam 118. The analysis of the genetic variance and the combining ability of the FL and ML. In the FL and the ML, h2N was less than h2B. GCA and SCA were large. RCA was little. Hl/D and (Hl/D)1/2 ere large. Heterosis was large. The r was in the negative direction, because the dominance genes were less expressed. The regression of the characters of FL and ML were appeared overdominant. The dominance in the FL of parents ere in the order of Jam 120>Jam 114>Jam 119>Jam 119>Jam 118>Jam 107>Jam 117>Jam 113, and the ML of them in the order of Jam 114>Jam 108>Jam 120>Jam 117>Jam 118>Jam 107>Jam 119>Jam 113. The analysis of the genetic variance and combining ability of the FR and the MR. In the FR and the Mr, h2N was less than h2B. GCA was large. The SCA and RCA were little. In the FW, Hl/D was large but (Hl/D)1/2 was a little. In MR, Hl/D and (Hl/D)1/2 both were a littel. Heterosis was a little. E in the FR was in the negative direction, because the dominance genes were less expressed but that in the MR was the positive direction because the recessive genes were mainly expressed. The order of the dominance in the FR of the parents were in the order of Jam 117>Jam 114>Jam 108>Jam 120>Jam 118>Jam 119>Jam 107>Jam 113 and that in the MR these were in the order of Jam 114>Jam 117>Jam 108>Jam 118>Jam 107>Jam 119>Jam 120.

• Clinical and Experimental Reproductive Medicine
• /
• v.23 no.1
• /
• pp.41-49
• /
• 1996

본 연구는 체외수정에 의해 생산된 생쥐 배반포기배를 vitrification 방법으로 동결보존하였을때 높은 생존율을 얻기 위한 적정조건을 검토하고자 실시하였다. 배반포기배를 생산하기 위하여, B6CBA F1 (C57BL/6, (표현불가)${\times}CBA/N$, (표현불가)) 계통의 생쥐 미수정란에 $1{\times}10^6$ spermatozoa/ml 농도의 정자로서 수정을 유도하였으며, 이후 $37^{\circ}C$, 5% $CO_2$배양기내에서 96시간동안 체외배양하였다. 배양 4일째의 배반포기배는 발달상태에 따라 early, middle 그리고 hatching blastocysts로 구분하였다. 본 실험에 사용된 동결보존액은 30% Ficoll과 0.5mol의 sucrose가 첨가된 mDPBS 용액에 40%의 ethylene glycol를 첨가한 EFS 40 (Zhu et al., 1993) 이었고, 수정란은 $25^{\circ}C$의 상온에서 먼저 20% ethylene glycol에 노출된 후 EFS 40 용액으로 옮겨 액체질소에 침지하는 2단계 동결법에 의해 동결보존되었으며, 급속융해하여 다음과 같은 결과를 얻었다. 1. 체외수정율과 배양 4일째 배반포기까지의 배발달율은 각각 89.4%와 86.1%였다. 2. 20% ethylene glycol에서 5분간 평형된 후 EFS 40 용액에 냉동보존된 후 융해된 난자의 생존율은 20% ethylene glycol에 0, 1, 3분간 평형된 난자의 생존율에 비해 유의하게 높았다. 3. 배반포기배를 20% ethylene glycol에서 5분간, EFS 40 용액에 1분간 차례로 노출한 다음 체와배양하였던 바, 배양 24시간째 생존율은 $82.9%{\sim}88.4%$ 였다. 본 연구 결과, 체외수정, 배양된 생쥐 배반포기배는 20% ethylene glycol과 EFS40에 대한 노출만으로는 난자의 생존성에 나쁜 영향을 미치지 않는 것으로 미루어 보아 배반포기배의 초자화 동결이 가능함을 시사하였다. 따라서 동결 융해 후 높은 생존율은 상온에서 난자를 2단계 즉, 20% ethylene glycol에 5분간 평형시킨 후 EFS 40 용액에 노출하여 1분내에 LN2에 직접 침지하는 간편한 동결방법으로 얻을 수 있었다.

• Korean Journal of Medicinal Crop Science
• /
• v.18 no.4
• /
• pp.266-272
• /
• 2010

There is a considerable difference in morphological traits between Bokbunja cultivated in Korea (KCB) and Korea native Rubus coreanus, contrary to the conviction that the cultivated Bokbunja is the domestication of R. coreanus. To infer the phylogenetic relationship of KCB with other Rubus species, we compared the chloroplast DNA spacers of KCB with those of several Rubus species including black raspberry, R. occidentalis. The three chloroplast DNA spacers, atpB~rbcL, trnL~trnF, and trnT~trnL, were amplified using the specific primer pairs and converted to Single Strand Conformational Polymorphism (SSCP) markers. The SSCP makers of the chloroplast DNA spacers showed a considerable variation both within and among Rubus species. In the phylogenetic tree generated by the SSCP markers, KCB accessions were located in the same clade with R. occidentalis, but R. coreanus accessions in the different clade. Also, in the phylogenetic tree by the nucleotide sequences of the chloroplast DNA spacer trnL~trnF, KCB located in the same clade with R. occidentalis but not with R. coreanus. These results suggest that the three KCB accessions share higher similarity with R. occidentalis than with R. coreanus in the three chloroplast DNA spacers.

• Journal of Sericultural and Entomological Science
• /
• v.48 no.2
• /
• pp.61-67
• /
• 2006

We investigated fertilization rate, hatchability rate, pupation rate and cocoon quality etc to selecting good characteristics of the wild silkmoth, Antheraea yamamai using method of indoor-and outdoor-rearing. As results of indoor rearing, the healthiness of A. yamamai was better in light green colour strain than that of dark green colour strain, but cocoon quality was better in dark green colour strain. There was a little effect to selecting major characteristics in grade of A, B and C strain classified with cocoon weight. $F_1$ hybrid was lower in fertilization rate and hatchability rate than parent, but higher in pupation rate and cocoon quality. There was no effect of heterosis in $F_2$ hybrid. In case of outdoor rearing, mortality rate was lower than that of indoor rearing but missing larva was higher. In addition, outdoor rearing is higher that indoor rearing in cocoon weight, cocoon shell weight and cocoon shell rate.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.22 no.2
• /
• pp.85-93
• /
• 2008

In 22.9[kV]-y distribution systems, underground cables are provided with 3-wire loop multiple-point ground in which each coaxial-neutral line of the distribution cable lines(A, B, C phases) is 3-wire common grounded at every connecting section. In the underground cable distribution systems, circulating current flows in the coaxial-neutral lines and its magnitude amounts to about $40{\sim}50[%]$ load currents, even though loads are balanced. This paper presents a new ground method to overcome such a problem and a comprehensive analysis in tows of current capacity of power cables, induced voltage of cable sheath, and electromagnetic interference voltage from power cable lines.

• Korean journal of applied entomology
• /
• v.19 no.2 s.43
• /
• pp.109-112
• /
• 1980

This study was carried out to investigate the distribution of egg parasitoids from green rice leafhopper, Nephotettix cincticeps Uhler, from the middle of August through the early September in southern rice cultural areas. The results were obtained as follow. 1. Four species of egg parasitoids, Gonatocerus sp. A and B, Paracentrobia andoi and Anagrus sp. are newly recorded from the eggs of green rice leafhopper. 2. Egg parasitism rates of Gonatocerus sp. were varied from $41.0\~73.9\%$ on an egg mass basis and from $29.7\~49.0\%$ on basis of egg. Percent parasitism rates by Paracentrobia andoi were $0\~9.7\%$ on an egg mass basis and from $2.1\~12.6\%$ on basis of egg. Gonatocerus sp. was dominant egg parasitoid in the parasitism rate than Paracentrobia andoi.

• Korean Journal of Poultry Science
• /
• v.38 no.1
• /
• pp.5-12
• /
• 2011

In this study, we analyzed the mitochondrial DNA D-loop region of Korean native chicken to clarify their phylogenetic relationships, possible maternal origin and routes of introduction into Korea. A 1231-1232 bp DNA fragment from the mtDNA D-loop region was sequenced in 315 chickens from 11 populations, Thirty-five variable sites that defined 21 haplotyes were observed. In Korean native chicken, diversity accounted for 90% of the variation, little differentiation among the strains. The 21 haplotypes clustered into 5 clades which were A, B, C, D and E. These results indicate that Korean chickens were derived from China with multiple origins.

• Journal of Life Science
• /
• v.18 no.12
• /
• pp.1665-1670
• /
• 2008

Soybean oil is an important source of vegetable oil for human food and nonfood applications and accounts for approximately 22% of the world's total edible oil production. Improvement of the quality and quantity of soybean seed oil constituents is one of the most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control oleic, linoleic, and linolenic acid contents in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.85, 0.82 and 0.81, respectively. Eight independent QTLs for oleic acid content were identified from linkage group (LG) A2, C1, D2, F, G, L, and O. Seven QTLs for linoleic acid content were located on LG D1b, E, H, I and L. Oil content was related with five QTLs located on LG C1, H, J, K, and L. Oleic, linoleic, and linolenic acid have two common QTLs on LG C1 and L. Thus, we identified major loci improving soybean oil quality.

• Journal of Life Science
• /
• v.14 no.2
• /
• pp.229-234
• /
• 2004

Feathers, which are almost pure keratin protein, are produced in large amounts as a waste by-product at poultry-processing plants. Keratinolytic enzymes may have important uses in biotechnological processes involving keratin-containing wastes from poultry and leather processes. In this study, screening and identification of keratin-degrading bacteria were investigated. Five keratin-degrading bacterial strains (F3-1, F3-4, F7-1, C1-1, C1-2) were isolated from compost and decayed chicken feather. On the basis of morphological, physiological studies, and Biolog system, all isolates were identified as the genus Bacillus. Among them, the strain F7-1 had the highest feather-degrading activity and was selected for further taxonomical study. Phylogenetic analysis of strain F7-1 based on comparison of 165 rDNA sequences revealed that this strain is closely related to Bacillus megaterium.

• Proceedings of the KIEE Conference
• /
• 2006.07b
• /
• pp.1159-1160
• /
• 2006

As the grid-connected photovoltaic power conditioning systems (PVPCS) are installed in many residential areas, this has raised potential problems of network protection on electrical power system. One of the numerous problems is an Island phenomenon. There has been an argument that because the probability of islanding is extremely low it may be a non-issue in practice. However, there are three counter-arguments: First, the low probability of islanding is based on the assumption of 100% power matching between the PVPCS and the islanded local loads. In fact, an island can be easily formed even without 100% power matching (the power mismatch could be up to 30% if only traditional protections are used, e.g. under/over voltage/frequency). The 30% power-mismatch condition will drastically increase the islanding probability. Second, even with a larger power mismatch, the time for voltage or frequency to deviate sufficient to cause a trip, plus the time required to execute the trip (particularly if conventional switchgear is required to operate), can easily be greater than the typical re-close time on the distribution circuit. And, third, the low-probability argument is based on the study of PVPCS. Especially, if the output power of PVPCS equals to power consumption of local loads, it is very difficult for the PVPCS to sustain the voltage and frequency in an island. Unintentional islanding of PVPCS may result in power-quality issues, interference to grid-protection devices, equipment damage, and even personnel safety hazards. So the verification of anti-islanding performance is strongly needed. In this paper, the authors propose the improved RPV method through considering power quality and anti-islanding capacity of grid-connected three-phase PVPCS in IEEE Std 1547 ("Standard for Interconnecting Distributed Resources to Electric Power Systems"). And the simulation and experimental results are verified.