• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.218-221
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• 2010

'Anyu', a perilla (Perilla frutescens (L.) Britton) cultivar for edible seed was developed by the Department of Functional Crop Science, National Institute of Crop Science, RDA in 2008. It was originated from the cross between a pedigree of YCPL1824 as a female and SF9225-3-1 as a male parent in 1998. 'Anyu' can be characterized by purple flower color and brown grain color. 'Anyu' showed semi-dwarft type with 120 cm height, and has lodging tolerance. Maturing date of 'Anyu' was September 24, which is 15 days faster than that of 'Saeyeopsil'. The early maturity of this new cultivar showed a great advantage to various planting system with other crops. This new cultivar has high oil content (45%) and high linolenic acid in the fatty acid composition. The yield potential of 'Anyu' was about 0.9 ton/1ha in the regional yield trial.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.168-172
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• 2009

'Punghan' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2008. It was derived from a cross between 'Beltsville 61-150 (IT133501)' and $F_1$ between 'Sikyonggwiri' and 'PA202-210'. A oat cultivar from USA, 'Beltsville 61-150', has a high cold tolerance, while the $F_1$ between 'Sikyonggwiri' and 'PA202-210' has early heading and high yielding with large-size grain. Subsequent enerations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO99013-B-YB-31', was selected for earliness and good agronomic characteristics and designated as a line name of 'Gwiri 60'. The line 'Gwiri 60' was subsequently evaluated for winter hardiness, earliness, and forage yield in five locations, Yesan, Cheongwon, Iksan, Kimjae, and Jeju, from 2006 to 2008 and finally named as 'Punghan'. Over 3 years, the average forage dry matter yield of 'Punghan' harvested at milk-ripe stage was $14.5\;ton\;ha^{-1}$, compared with $13.6\;ton\;ha^{-1}$ of check cultivar 'Samhan'. 'Punghan' is about 2 days earlier in heading date than check cultivar. 'Punghan' is adapted primarily for winter planting use in the mid-southern regions of Korean peninsula.

• Journal of Life Science
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• v.30 no.12
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• pp.1063-1069
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• 2020

Two crucian carp species (Carassius auratus and C. cuvieri) inhabit Lake Yedang in South Korea, and C. auratus is known to be native to Korea. Classification of these two freshwater fish species is often confused because of their morphological similarity. To distinguish the two species, we conducted phylogenetic and population genetic analyses of C. auratus and C. cuvieri based on their mitochondrial DNA sequences of the cytochrome b gene (CYTB). We also compared our partial CYTB sequence (<1,056 bp) with 10 Chinese, nine Japanese, and two Russian crucian carp fishes. The results of our phylogenetic analysis showed that C. auratus and C. cuvieri were clearly divided into two phylogroups. The nucleotide diversity (π) of C. auratus from Korea, China, and Japan showed a range of 0.146%~0.421%, while the range of π of C. cuvieri from Korea and Japan was lower than those of C. auratus (0.0%~0.054%). Moreover, the comparison of CYTB divergence among crucian carp fishes in China, Japan, and Korea indicated that Korean Carassius fishes were distantly related to those from China and Japan, with two exceptions: the pairwise Fst value between Korean C. auratus and northern Chinese C. auratus was not significantly different. In addition, no significant genetic divergence between Korean and Japanese C. cuvieri was detected. We conclude that, despite the morphological similarities, C. auratus and C. cuvieri should be considered as separate freshwater fish resources in conservation efforts for genetic diversity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.199-202
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• 2000

The leaf color varies with the contents of pigments, especially chlorophylls and carotenoids. Teichung 65 (T.65), a japonica rice, with pgl(pale-green leaf) gene exhibits pale green color on the whole plant from seedling to harvest. This study conducted to evaluate the agronomic characters and examine the chloroplasts of 'pgl' plants in parents and BC$_1$F$_2$ of T.65(pgl) xSuweon ${345}^2$. The average grain yield of pale-green-leaf individuals in F$_2$ was the same as T.65(pgl) but that of green-leaf individuals was much higher than that of Suweon 345. The contents of chlorophyll a(Ca), chlorophyll b(Cb) and total chlorophyll content(Ct) of T.65(pgl) in flag leaf were lower than those of Suweon 345, but the Ca/Cb ratio of T.65(pgl) was higher than that of Suweon 345 during from 15 days after heading (DAH) to 60 DAH. The SPAD value of T.65(pgl) in flag leaf was lower than that of Suweon 345, but that in the second and the third leaves was similar to that of Suweon 345. The SPAD value of pale-green-leaf individual group was lower than that of green individual group in upper three leaves. The structural difference of chloroplasts in flag leaf between T.65(pgl) and Suweon 345 through TEM at 20 DAH was not detected, but the number of osmium granules in chloroplast of T.65(pgl) were higher than that of Suweon 345.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.94-101
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• 2001

The correlation among days to germination, days to flower, days to seed ripening and number of seeds per a pod and their combining were studied in 15 F₁s from the partial six-parent diallel cross in Petunia hybrida. The days to flowering, days to germination and seed number per a pod showed high positive correlation together, however germination percentage and number of seed showed negative correlation with days to ability(SCA) were highly significant for all the characters. The estimates of variance components of SCA were greater than those of GCA for all the characters, showing preponderance of non-additive gene actions for these characters. The line of A and C showed relatively high GCA effect for days to germination and days to flower and the lines of C and G for germination, days to seed ripening and number of seeds per a pod. The crosses of D×G and E×F exhibited high SCA effect for decreasing days to flower and days to germination, and increasing germination percentage and number of seeds per a pod. The broad sense heritability was generally high compared to narrow sense one in the 5 characters. The higher heritability values of both broad and narrow sense were shown in the days to ripening and number of seeds per a pod.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.113-118
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• 2006

The composition of fatty acids, minerals, total dietary fiber and vitamin $B_1,\;B_2$, in pigmented rice varieties were determined. Proximate composition and color were also compared among pigmented rice varieties. Crude protein contents of black rice were higher than those of red and white rice, especially, C3GHi line had the highest protein content. There were no significant differences in lipid and crude ash contents between pigmented and white rice. Black rice showed lower Hunter value L and b value compared with red, green and white rice. But, Green rice showed lower Hunter value a compared with black rice. Green rice showed the higher contents in total dietary fiber, vitamin $B_1\;and\;B_2$ compared with white and black rice. The major fatty acids of pigmented rice were palmitic acid, oleic acid and linoleic acid. The contents of oleic acid was similar to that of linoleic acid in white rice. Oleic acid contents was lower than linoleic acid in black rice, but higher in red rice. Most mineral contents of pigmented rice except Fe, Zn and Mn were higher than those of white rice. Especially, Green rice had the highest mineral content.

• Journal of Life Science
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• v.25 no.2
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• pp.180-188
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• 2015

The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.

• Journal of the Korea Institute of Building Construction
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• v.18 no.1
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• pp.1-7
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• 2018

This work aims to suggest the most proper Hwangtoh color(ocher) for sidewalks and walkways located at the central part of the city. The actual colors of Hwangtoh samples that were obtained from 3 different locations (mountain walkways) in Busan were measured. In addition, personal preferences of such Hwangtoh colors were evaluated through survey in order to provide a proper standard reference color for urban walkways and sidewalks. With respect to the chromaticity of Hwangtoh obtained in Busan, $a^*$ and $b^*$ values were found to range from 3.6 to 13.6 and 11.4 to 23.4, respectively. It was also found that $L^*$ (lightness) values of 3 different Hwangtoh samples were found to be similar. According to the results from survey, higher values of $+a^*$ (red type) and $+b^*$ (yellow type) were preferred, indicating the red and yellow color needs to be developed stronger from Hwangtoh. Based on these findings, the proper Hwangtoh color used for walkways in urban area was chosen. It was found to be $L^*$ for 32.2~45.8, $a^*$ for 5.2~13.6, and $b^*$ for 15.8~21.2, respectively.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.431-435
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• 2009

This study was conducted to compare the effect of various LED and UVa light to enhanced coloration of paprika fruits cultivars; 'Special', 'Helsinki'. Immatured paprika fruits were irradiated with red LED, blue LED and UVa lights which were high intensity (red: $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, blue: $70{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, UVa: $3{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and low intensity (red: $20{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, blue: $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, UVa: $0.25{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The coloration of 'Special' cultivar was increased the fastest in high intensity UVa light for 2 days after treatment. There were no significantly difference among treatments except the low intensity UVa light. The coloration of 'Helsinki' was increased the fastest in blue LED light, and followed by red LED light and UVa light for 2 days after treatment. The $a^*/b^*$ value of 'Special' and $b^*$ value of 'Helsinki' that indicated the paprika fruit coloration were generally higher high intensity LED and UVa lights than low intensity LED and UVa lights. We couldn't find out any significantly different coloration effect among all treatments in final days of coloration. and rather than UVa treatment fruit shrunk the surface of paprika fruits.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.93-105
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• 2015

This study was performed to analyze genetic relationships of the four major cucurbitaceous crops including watermelon, melon, cucumber, and squash/pumpkin. Among 120 EST-SSR primer sets selected from the International Cucurbit Genomics Initiative (ICuGI) database, PCR was successful for 51 (49.17%) primer sets and 49 (40.8%) primer sets showed polymorphisms among eight Cucurbitaceae accessions. A total of 382 allele-specific PCR bands were produced by 49 EST-SSR primers from 24 Cucurbitaceae accessions and used for analysis of pairwise similarity and dendrogram construction. Assessment of the genetic relationships resulted in similarity indexes ranging from 0.01 to 0.85. In the dendrogram, 24 Cucurbitaceae accessions were classified into two major groups (Clade I and II) and 8 subgroups. Clade I comprised two subgroups, Clade I-1 for watermelon accessions [I-1a and I-1b-2: three wild-type watermelons (Citrullus lanatus var. citroides Mats. & Nakai), I-1b-1: six watermelon cultivars (Citrullus lanatus var. vulgaris S chrad.)] a nd C lade I -2 for melon and cucumber accessions [I-2a-1 : 4 melon cultivars(Cucumis melo var. cantalupensis Naudin.), I-2a-2: oriental melon cultivars (Cucumis melo var. conomon Makino.), and I-2b: five cucumber cultivars (Cucumis sativus L.)]. Squash and pumpkin accessions composed Clade II {II-1: two squash/ pumpkin cultivars [Cucurbita moschata (Duch. ex Lam.)/Duch. & Poir. and Cucurbita maxima Duch.] and II-2: two squash/pumpkin cultivars, Cucurbita pepo L./Cucurbita ficifolia Bouche.}. These results were in accordance with previously reported classification of Cucurbitaceae species, indicating that watermelon EST-SSRs show a high level of marker transferability and should be useful for genetic study in other cucurbit crops.