• Title/Summary/Keyword: B 계통

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Studies on the Karyotypes and Comparative DNA Values in Several Korean Cyprinid Fishes (한국산 담수어류(잉어과) 몇종의 핵형과 DNA 상대량에 관한 연구)

  • Kang, Yung-Sun;Park, Eun-Ho
    • The Korean Journal of Zoology
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    • v.16 no.2
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    • pp.97-108
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    • 1973
  • Karyological characteristics and relative DNA values were investigated in four species of the family Cyprinidae of Korean fresh-water. The results obtained were as follows; 1) The two Hemibarus species, H. longirostris and H. labeo, had the aiploid chromosome number of 50. The compliment of former consisted of 7 metacentric, 14 submets- and subtelocentric and 4 telo- or acrocentric pairs, whereas latter had 9, 11 and 5 pairs respectively, Total arm numbers (AN) were 92 and 90. 2) In the case of two species of genus Moroce, M. oxycephalus and M. lagowskii, their diploid number and karyotype showed in identical pattern for each other (2n=50); 6 pairs of metacentrics, 14 pairs of submeta- and subtelocentrics and 5 pairs of telo- or acrocentrics. The AN of both species were 90. 3) Relative DNA values of all species were measured as about 60% that of gold fish. From the above observation, the cytogenetical character was discussed with special concern to the phylogenetic significance.

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Study on the Increase of Egg Weight and Early Selection based on Part Record in Layer (산란종계의 란중 증대성과 조기 선발에 관한 연구)

  • Park, Y.H.;Ohh, B. K.
    • Korean Journal of Poultry Science
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    • v.10 no.2
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    • pp.75-81
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    • 1983
  • The records on 787 white Leghorn hens from two different strains, A and K were wed in this study to find out the pattern of increase in egg weight with age and the optimum age of early selection for maximizing the genetic gain. The pattern of increase in egg weight with age in two lines which was expressed on graph showed that the amount of increase was larger in the early part of the laying year and it decreased gradually. Regression equations suggested that egg weight increased linearly with age at the rate of 0.46 gram per week for line A and 0.83 gram for line K. And in the regression analyses of each period mean egg weight on annual mean egg weight, the coefficients of EW 30 that is mean egg weight from 29 weeks of age to 32 weeks of age were 0.86 for line A, 0.88 for line K, and those were the highest figures among the all regression coefficients of each mean egg weight on annual mean egg weight. In estimating the relative selection efficiency of each period mean egg weight on annual mean egg weight in EW 46, selection on EW 34 for line A and on EW 30 for line K was the most efficient for improving egg weight. These results suggested that one periods from 29 weeks of age to 36 weeks of age could be used for the best predictable time for improving annual mean egg weight.

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Detection of Soybean mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification (Reverse transcription Loop-mediated isothermal amplification을 이용한 Soybean mosaic virus의 진단)

  • Lee, Yeong-Hoon;Bae, Dae-Hyeon;Kim, Bong-Sub;Yoon, Young-Nam;Bae, Soon-Do;Kim, Hyun-Joo;Mainali, Bishwo P.;Park, In-Hee;Lee, Su-Heon;Kang, Hang-Won
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.315-320
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    • 2015
  • Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at $58^{\circ}C$ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

Estimation of Genetic Parameters on Pre-weaning Growth Traits in Hanwoo (한우의 이유전 성장형질에 관한 유전모수 추정)

  • Hwang, J.M.;Choi, J.G.;Kim, H.C.;Choy, Y.H.;Lee, C.;Yang, B.K.;Shin, J.S.;Kim, Jong-Bok
    • Journal of Animal Science and Technology
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    • v.49 no.2
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    • pp.171-176
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    • 2007
  • A total of 4,627 records on pre-weaning growth traits, birth weight(BW), weaning weight(WW) and pre-weaning daily gain(ADG) of Hanwoo’s born form 1970 to 2005 were collected from Hanwoo Experiment Station, National Livestock Research Institute. Variance components and heritabilities of BW, WW and ADG were estimated with two models; Model 1 included only direct genetic effect while Mode1 2 included direct genetic, maternal genetic and permanent environmental effects. Direct herit- ability estimates of BW, WW and ADG estimated by model 1 were 0.34, 0.26 and 0.20, respectively. In model 2, direct heritability and maternal genetic heritability estimates were 0.14 and 0.06 for BW, 0.07 and 0.04 for WW and 0.05 and 0.04 for ADG. Estimates of genetic correlation between direct and maternal effect for BW, WW and ADG were 0.58, 0.75 and 0.61, respectively. Genetic correlation coefficients between BW and WW, between BW and ADG, and between WW and ADG were 0.76, 0.62 and 0.99, respectively.

Breeding of a New Spray Chrysanthemum Cultivar, 'Dream Round' with Dark Pink Petals and Thick Stem of Anemone Type for Cut-flower (줄기가 강건하고 진분홍색인 아네모네형 절화용 스프레이 국화 신품종 '드림라운드' 육성)

  • Jung, Yun Kyung;Kim, Sung Kee;Kim, Hee Dong;Lee, Young Soon
    • Horticultural Science & Technology
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    • v.31 no.4
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    • pp.517-521
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    • 2013
  • A new cultivar Dendranthema grandiflourm 'Dream Round' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2010. The variety 'Dream Round' was initially derived from a cross in 2006 between 'Hebo' a seed parent, a spray chrysanthemum cultivar with white anemone type, and 'Samos' as a pollen parent, a spray chrysanthemum variety with white anemone type. The cultivar has anemone type with white petals. Trial evaluation was conducted from 2008 to 2010 for the selection of that cultivar, including a shading culture in spring and a retarding culture in summer. The flowering time of 'Dream Round' was October 24th, and year-round flowering was possible by shading or lighting treatment. The diameter of flower of 'Dream Round' was 34.1 mm. Numbers of flowers per stem and petals per flower of 'Dream Round' were 12.8 and 26.1, respectively. Its leaf color was green (Green Group 147A) and plant height was 92.4 cm. Days to flowering of 'Dream Round' under the short day treatment was about 54 in spring, and diameter of flower of 'Dream Round' was 34.2 mm in the summer. The vase life of 'Dream Round' was 21.7 days in autumn.

An Experimental Study on Flow Distributor Performance with Single-Train Passive Safety System of SMART-ITL (SMART-ITL 1 계열 피동안전계통을 이용한 유동분사기 성능에 대한 실험연구)

  • Ryu, Sung Uk;Bae, Hwang;Yang, Jin Hwa;Jeon, Byong Guk;Yun, Eun Koo;Kim, Jaemin;Bang, Yoon Gon;Kim, Myung Joon;Yi, Sung-Jae;Park, Hyun-Sik
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.124-132
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    • 2016
  • In order to estimate the effect of flow distributors connected to an upper nozzle of CMT(Core Makeup Tank) on the thermal-hydraulic characteristics in the tank, a simplified 2 inch Small Break Loss of Coolant Accident(SBLOCA) was simulated by skipping the decay power and Passive Residual Heat Removal System(PRHRS) actuation. The CMT is a part of safety injection systems in the SMART (System Integrated Modular Advanced Reactor). Each test was performed with reliable boundary conditions. It means that the pressure distribution is provided with repeatable and reproducible behavior during SBLOCA simulations. The maximum flow rates were achieved at around 350 seconds after the initial opening of the isolation valve installed in CMT. After a short period of decreased flow rate, it attained a steady injection flow rate after about 1,250 seconds. This unstable injection period of the CMT coolant is due to the condensation of steam injected into the upper part of CMT. The steady injection flow rate was about 8.4% higher with B-type distributor than that with A-type distributor. The gravity injection during hot condition tests were in good agreement with that during cold condition tests except for the early stages.

Development of a psychrophilic-SCAR marker for Pleurotus eryngii (큰느타리버섯의 저온적응성 형질에 관련된 SCAR Marker 개발)

  • Kim, Su Chul;Hwang, Hye Sung;Cho, Yun Jun;Kim, Hye Su;Ryu, Jae-San;Cho, Soo Jeong
    • Journal of Mushroom
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    • v.11 no.3
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    • pp.171-176
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    • 2013
  • Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specific DNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilic strain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basis of the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearly distinguish the psychrophilic strains from the control strains.

'Asian Flame' an Asiatic Hybrid Lily with Unspotted Red Petals (무반점 적색 아시아틱 나리 '아시안플레임' 육성)

  • Rhee, Hye-Kyung;Cho, Hae-Ryong;Lim, Jin-Hee;Kim, Mi-Seon;Park, Sang-Kun;Shin, Hak-Ki;Joung, Hyang-Young
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.519-521
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    • 2010
  • An Asiatic lily cultivar 'Asian Flame' was bred in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The crossing was conducted between female parent A94-34 ('Montreux' ${\times}$ 'Anabelle') and male parent 'Fire' by stigmatic pollination at Suwon in 1996. First selection was done and tentatively named as A98-98 in 1998. After in vitro multiplication and bulb production of A98-98 line, growth and flowering characteristic tests has been conducted from 2005 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS in 2007 in Taean. The new cultivar 'Asian Flame' flowers in middle of June and grows to an average of 122.7 cm in height. Flowers bloom upward-facing, red (RHS 45B) petals. Year-round flowering is possible by storage of the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales of large bulb to prevent leaf scorch.

A New Soybean Cultivar 'Daewang' with High Quality and Large Seed Size (장류용 고품질, 대립 콩 신품종 '대왕')

  • Choi, Jae-Keun;Ha, Keon-Soo;Byun, Hak-Soo;Heo, Nam-Ki;Jang, Eun-Ha;Kim, Sang-Soo;Lee, Se-Jong;Sa, Jong-Goo
    • Korean Journal of Breeding Science
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    • v.43 no.3
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    • pp.177-179
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    • 2011
  • A new soybean cultivar 'Daewang' was released by Agricultural Research & Extension Services, Gangwon Province in 2008. The Goals of breeding the variety was for large seed size, high yield, lodging tolerance and resistance to diseases such as soybean mosaic virus (SMV) and bacterial pustule. 'Daewang' was derived from the cross of GWS91, which has yellow seed coat, large seed size and late maturity, and 'Seokryang-putkong', which has large seed size and early maturity. The preliminary, advanced and regional yield trials for evaluation and selection were carried out from 2002 to 2007. It has a determinate growth habit with purple flower, yellow seed coat, yellow hilum and large seed weight (33.6 g per 100 seeds). The maturity date of 'Daewang' is 16 days later than the 'Taekwang'. It has a high content of total isoflavone ($1,851{\mu}g/g$). The average yield of 'Daewang' was 2.68 MT/ha in the regional yield trials (RYT) carried out in four locations in Gangwon province from 2004 to 2007 which was 4 percent higher than the check cultivar 'Taekwang'.

A New Green-Kerneled Glutinous Rice Mutant Variety, "Nogwonchalbyeo" Developed by Gamma Ray Irradiation (녹색찰현미로 이용 가능한 "녹원찰벼"의 돌연변이 육종)

  • Kang, Si-Yong;Shin, In Chul;Kim, Dong Sub;Lee, Geung-Joo;Kim, Jin-Baek;Lee, Deok Yul;Lee, Sang Young;Lee, Dong Jin
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.303-307
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    • 2008
  • We bred a new green-kerneled glutinous rice variety that can be cultivated in the whole area of Korea, because only one native green-kerneled glutinous rice cultivar, "Saengdongchalbyeo", has been cultivated in the southern coastal area due to its late heading. The seeds of "Saengdongchalbyeo" were irradiated with 200 Gy of gamma ray in 1995. A promising mutant variety, "Nogwonchalbyeo" ("Wonnong 17") was selected through line selection and regional yield trials. In particular, the new variety revealed at the earlier mid of August compared to that of "Saengdongchalbyeo", the early of September, and it was considerably tolerant to a field lodging due to its shortened culm length. Also, "Nogwonchalbyeo" had a higher ripened grain ratio and 1,000 grain weight compared to the original variety. The brown grain yield of the new variety was about 5.40 MT/ha, which was 11.3% higher than that of the original variety, in the regional yield trials at 3 different fields during 2000~2001. The brown and milled grains of the new rice variety contained 20 to 65% higher amount of total amino acids, respectively than that of the original and two checks. For chlorophyll -a, -b and total chlorophyll, the new variety showed nearly two-fold higher than the checks, and for the carotenoid, it had 5.3 - 7.6 times higher amount. These results showed that the new variety can be cultivated as a special green-kerneled glutinous rice with high functional compounds.