• Journal of Fisheries and Marine Sciences Education
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• v.26 no.3
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• pp.543-552
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• 2014

Papain family중 하나인 cysteine protease는 근골격계 질환 치료를 위한target molecule로 인식 되어왔으며 Cathepsin B 는 단백질 분해의 초기과정에 관여하는 cysteine proteases 중 하나이다. 본 연구는 넙치의 cathepsin B 유전자의 발현 양상과 넙치 cathepsin B(PoCtB)의 클로닝, 발현 및 효소특성을 분석하였다. cDNA Library Screening을 이용하여 넙치의 cDNA를 클로닝하였다. 넙치의 동정된 cathepsin B 유전자는 993bp의 open reading frame과 330개의 아미노산으로 이루어져있다. Cathepsin B의 propeptide region 내에 GNFD motif와 occluding loop 가 존재함으로써 이것이 명백하게 cathepsin B group이라는 것을 보여주며, 계통 유전학적 분석 결과 다른 종의 cathepsin B에 비해 초창기에 분화되어 나온 것으로 사료된다. mature enzyme인 maPoCtB은 fusion protein인 glutathione S-transferase를 포함하는 pGEX-4T-1 vector에 삽입하여 E.coli 균주인 $DH5{\alpha}$ 내에 발현시켰다. 재조합 단백질인 PoCtB을 과발현 시킨 결과 53kDa의 분자량을 가진다. 넙치 cathepsin B 활성은 Z-Arg-Arg-AMC와 같은 fluorogenic 펩타이드 기질을 이용하여 측정되었고 적정 pH는 pH.7.5 이다.

• Korean Journal of Plant Resources
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• v.8 no.1
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• pp.47-54
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• 1995

The ergot fungus requiers a rather long time for the incubation period for a disease and fungus tends to obstract the formation of the seeds. In order to do an experiment inoculated, a spore of ergot fungi into the florets was done 2-3 days before flowering. As a result, each trisomic types recognized the infection rate to be 90% to 100% so the trisomies of complete immunity was non existent in durum wheat, Triticum durum var. hordeiforme. However, the growth rate of large sclerotium than seeds differed mutually with each trisomic types. This tend to be sensitivity on the trisomies which has been the extra chromosomes of 2A, 4A and 7B in comparison with normal plant but the trisomies related to Tri-6A line was not forming the sclerotium of large size than seeds and grew outside the florets. Consequently, the resisitant gene against ergot fungi was considered to be existing on the chromosome 6A. The ergot fungi requires a long time for the incubation period until it is taken away from infection. The essential effect of resistant gene did not surmise on the chromosome 6A that gene concerned with the physiological effect to restrain the growing up of a ergot fungi in the growing process of plants.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.29-34
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• 2003

It has been well documented that white egg layers are far more resistant to fowl typhoid than the brown egg layers. In Korea, however, most consumers prefer brown eggs to white ones. Therefore, a study was conducted to Produce fowl typhoid-resistant crossbred layers producing somewhat brown-colored eggs. Several crossbred strains were obtained from crossbreeding white egg lines (W) with brown egg lines (B). These crossbred layers (W${\times}$B) produced eggs with varying degrees of brown-colored shells between the white eggs obtained from W (White) and the brown eggs from B (Brown). Eggs from the peak stage of production were collected and their eggshell color values were measured. The mean eggshell color values of White and Brown were 81.9 and 36.4, respectively. Eggs from the crossbred lines (W${\times}$B) were collected, and their eggshell color values were measured to re-group these eggs according to their color. The mean eggshell color values of Trt-White, Middle, and Trt-Brown were 70, 60, and 50, respectively (Fig. 1). A total of 247 people living in Daejeon area, mainly housewives, took part in this survey. First, they were offered eggs with varying degrees of eggshell color in a paper egg-tray, together with a questionnaire. After they filled out the first questionnaire, they were instructed that the eggshell color has nothing to do with its nutritive value. In the second questionnaire, their preference on both eggshell color and price, i.e., purchasing will, were investigated. In the first questionnaire, the Brown (eggshell color lightness 36.4) were most preferred, and the Trt-white (eggshell color lightness 70) were least preferred. No statistical significance was detected between Brown and Trt-Brown, and White and Trt-White. In the second questionnaire, the trend was the same as in the first. Although no significant difference was found between Trt-Brown and Brown, however, the Trt-Brown were most preferred, surpassing the Brown. In conclusion, regardless of the nutritive values, the Korean consumers prefer brown eggs to white ones, and this trend could be changed gradually through consumer education.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.600-605
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• 2011

'Boseogheugchal', a new black pericarp glutinous rice cultivar (Oriza sativa L.), was developed by the rice breeding team of National Institute of Crop Science, RDA, Suwon, Korea during the period from 1997 to 2008 and released in 2008. It was derived from a cross between SR18638-B-B-B-18-2/ Heugmi H31. The maturity of this cultivar is about 135 days from seedling to heading. This cultivar has about 12 tillers per hill and 94 spikelet numbers per panicle. Ripening ratio is about 72% and 1000 grain weight is 21.6 g in brown rice. This new variety shows grain shattering resistance and susceptible for blast, bacterial leaf blight and stripe virus. 'Boseogheugchal' has glutinous endosperm and low protein contents. It has higher anthocyanin content compared to black pigmented check cultivar 'Hegjinjubyeo'. The yield performance of brown rice was 4.82 MT/ha in local adaptability test for three years. 'Boseogheugchal' is adaptable to central plain area of Korea.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.05a
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• pp.953-954
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• 2012

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.217-219
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• 1998

Of over 100 accessions of pepper tested, MC 4 and MC 5, PBC631, PI322727, PI358812, PBC066, Kerting, PI322726, PI369994, PI377688, CMS-B, PI322728, and Jatilaba showed similarly high level of resistance to bacterial wilt (Ralstonia solanacearum).

• Proceedings of the Korean Society of Crop Science Conference
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• 1992.05a
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• pp.122-123
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• 1992

• Proceedings of the Korean Nuclear Society Conference
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• 1998.05a
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• pp.653-659
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• 1998

국내의 첫액금로가 될 KALIMER의 안전등급 잔열제거 계통인 PSDRS의 설계 및 설계/해석방법론 설정을 위하여 기존의 PSDRS 해석 코드인 기존의 PARS를 개선시킨 PARS2-iB를 개발하고 이를 이용하여 PSDRS내부 전열모드의 정성적 및 정량적 특성, 그리고 PSDRS 설계에서의 우선 고려 대상 설계인자 및 PSDRS 설계 해석 방법론 설정을 위한 PSDRS 작동 mechanism의 열유체적 연결 특성을 파악하였다.

• Proceedings of the Korean Society of Crop Science Conference
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• 1989.05a
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• pp.60-61
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• 1989

• Korean Journal of Poultry Science
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• v.38 no.2
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• pp.137-144
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• 2011

The current work was carried out to investigate the effect of crossbred Korean native chickens (KNC) on fatty acid, amino acid and nucleotide-related compounds contents of chicken meat. A total of 360 male chicks (1d of age) was used in this work and were divided 4 groups as A) (KNC egg-meat type C strains ${\times}$ KNC meat type S strains) ${\times}$ Ross broiler, B) (KNC egg-meat type C strains ${\times}$ KNC meat type H strains) ${\times}$ KNC meat type S strains, C) (KNC native R strains ${\times}$ KNC meat type S strains) ${\times}$ KNC meat type H strains and D(White Semibroiler Chickens) strains for 5 weeks at the flat house. Palmitic acid and vaccenic acid were highest in C strain meat, and myristic acid and linolenic acid were lowest in A strain meat (p<0.05). Saturated fatty acid was lowest in C strain meats (p<0.05). Valine, leucine, phenylalanine and lysine of essential acid were low in A strain meat. Cystine, aspartic acid, glycine, alanine and proline were also low in A strain meat. Hypoxanthin (Hx) was high compared other strains at 5 weeks and low at 10 weeks. IMP was high compared other strains at 5 and 10 weeks. AMP has not significant difference among strains at 5 weeks but B strain was high other strains at 10 weeks. These results showed that C stain was excellent on the fact of nutrients compared to other strains. Consequently, the result of this work gave the basic data that needed to develope the new strains.