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Production of a Phospholipase C by Bacillus cereus and Its Characterization (Bacillus cereus에 의한 C형 인지질 분해효소의 생산과 특성 고찰)

  • 서국화;임용식;이종일
    • KSBB Journal
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    • v.19 no.4
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    • pp.250-256
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    • 2004
  • In this work we have cultivated several B. cereus strains in a complex LB medium in order to study the production of phospholipase C (PLC), and among them B. cereus 318 showed the highest productivity of PLC. Some components, i.e., 5 g/L glucose, 5 g/L yeast extract, 5 g/L peptone, 0.5∼1.0 g/L K$_2$HPO$_4$, 0.02∼0.04 g/L ZnSO$_4$$.$7H$_2$O and 3 g/L NaHCO$_3$ were found to be optimal for the high production of PLC by B. cereus 318. Optimal culture temperature and pH were found to be 30$^{\circ}C$ and pH 7.5 for the PLC production, respectively. Optimum reaction temperature and pH of the PLC produced by B. cereus 11 and 318 were 45$^{\circ}C$ and pH 4.0, while they were 50$^{\circ}C$ and pH 7.0 for the PLC by B. cereus 559. The PLC produced by B. cereus was activated by Mn$\^$2+/, Co$\^$2+/ and dimethyl sulfoxide (DMSO), but its activity was inhibited by Cu$\^$2+/ and partially by glycerol, isopropanol and sodium dodecyl sulfate (SDS).

Evaluation and Prediction of Color Changes of Sugar-Glycine Mixtures by Maillard Reaction (마이야르 반응에 의한 당과 글리신 혼합물의 색변화의 평가와 예측)

  • Kim, Woo-Jung;Chun, Young-Hye;Sung, Hyun-Soon
    • Korean Journal of Food Science and Technology
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    • v.18 no.4
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    • pp.306-312
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    • 1986
  • Color changes of a mixture of 0.8 M glucose, fructose or sucrose and 0.8 M glycine were evaluated by Hunter 'L', 'a', 'b' values during Maillard reaction at the temperature range of $70^{\circ}-100^{\circ}C$. The results suggested the reaction to be devided into three stages. In initial reaction the 'L' and 'a' values decreased slowely, and 'b' rapidly increased to maximum, which were followed by a rapid decrease in 'L' and 'b' and rapid increase in 'a' values at intermediate stage. At final reaction period the both 'L' and 'b' were slowed down in their reduction rate while 'a' decreased rapidly from its maximum point. All of the Hunter values were reached to nea. zero. Linear equations, which can be used for prediction of cole. change in Maillared reaction, of $1/\sqrt{(L_t-L_{\circ})^2\;+\;(a_t-a_{\circ})^2\;+\;(b_t-b_{\circ})^2}\;=\;a(1/t)\;+\;b$ for Hunter values and $1/\sqrt{X\;+\;Y\;+Z}\;=\;a(1/t)\;+\;b$ for CIE tristimulus values were found to have a very high correlation coefficient. Relationships between 'L' and 'a', 'L' and 'b' and 'a' and 'b' were also graphcally compared.

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Wake-up Treatments for Improving Oviposition and Colony Development of the Bumblebees Bombus ignitus and B. terrestris

  • Yoon, Hyung-Joo;Lee, Kyeong-Yong;Lee, Samg-Beom;Park, In-Gyun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.20 no.1
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    • pp.19-24
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    • 2010
  • Bumblebees are widely used to pollinate crops in greenhouses and fields. Here, we investigated whether different wake-up treatments during a short period of 1~3 days just before indoor rearing has any effects on oviposition and colony development of $CO_2$-treated Bombus ignitus queens and artificially hibernated B. terrestris queens The wake-up regimes were defined as 16L for 1 day (16L-1), 16 L per day for 3 days (16L-3), 24L for 1 day (24L-1), or 24D for 1 day (24D-1). Among these wake-up treatments, the oviposition rate and preoviposition period of B. ignitus queens reared at 24L-1 were 16.7~25.1% higher and 1.0~3.5 days shorter than other wake-up treatments. B. terrestris queens reared at 24L-1 also showed the best results for egg-laying characteristics, which were 8.9~18.8% higher for oviposition and 0.6~3.5 days shorter for preovipostion period than other wake-up treatments. Furthermore, B. terrestris queens reared at 24L-1 were 17.5% and 13.8% higher in rate of colony foundation and queen production, respectively, than other wake-up treatments. These results show that the most favorable wake-up treatment just before rearing for egg-laying and colony developmental characteristics of B. ignitus and B. terrestris queens was 24L-1. Overall, our findings indicate that a wake-up treatment just before rearing was effective for colony initiation and colony development of bumblebee queens.

Investigation of Pump Wavelength Dependence of Long-Wavelength-Band Erbium-Doped Fiber Amplifier using 1530nm-Band Pump (L 대역 EDFA 특성의 펌프 파장 의존성에 관한 연구)

  • Choi, Bo-Hun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.7
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    • pp.1249-1255
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    • 2008
  • 1530nm band has been studied as pump wavelength for long-wavelength-band erbium-doped fiber amplifier (L-band EDFA). The pump source is built using a tunable light source and cascaded conventional-band (C-band) EDFA. The L-band EDFA uses a forward pumping scheme. Within the 1530nm band, 1545nm pump demonstrates 0.45dB/mW gain coefficient, which is twice better than that of conventional 1480nm pumped EDFA. The noise figure of 1530nm pump is at worst 6.36dB, which is 0.75dB higher than that of 1480nm pumped EDFA. Such high gain coefficient indicates that the L-band EDFA consumes low power.

Studies on the Formation of L-Threonine by auxotrophic mutants of Brevibacterium flavum (Brevibacterium Flavum의 Auxotrophic Mutants에 의한 L-Threonine 생성(生成)에 관한 연구(硏究))

  • Lee, Kap-Rang;Park, Dong-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.4
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    • pp.251-261
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    • 1987
  • This study was attempted to increase the production of L-Threonine by Brevibacterium Flavum ATCC 14067, To select the strain which produce the highest threonine, mutants ere induced by N-methyl-N'-nitro-N-nitrosoguanidine treatment. The composition of media and cultural condition for its overproduction of threonine were also studied. In a threonine producer, strain B-13(Met-) was the strain producing the highest amount of threonige among methionine, lysine and isoleucine auxotrophs. The following results were obtained. 1. The wild strain and B-13(Met-) produced threonine 1.4mg/ml and 4.86mg/ml , respectively. 2. The optimum composition of medium for producing threonine by Brevibacterium Flavum B-13 was glucose 10%, ammonium sulfate 4%, potassium phosphate monobasic 0.2%, magnesium sulfate 0.05%, biotin $200{\mu}l$, thiamine $300{\mu}l$. Addition of nicotinic acid also led to increase L-threonine production. 3. In addition of organic nutrients to the fermentation medium, peptone n'ere effective and addition of methionine $100{\mu}g/ml$ produced the highest amount of L-Threonine. Aspartic acid and homoserine were also effective when these amino acid were added to the fermentstion medium. 4. Cultural conditon on threonine production by B-16 were investigated. The optimum pH was 7.0-8.0. The highest amount of threnine was produced after 4 days of cultural period.

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IL-1 gene polymorphisms in Korean periodontitis patients (한국인 치주염 환자에서의 IL-1 유전자 다변성 연구)

  • Nam, Seung-Ji;Chung, Hyun-Ju;Kim, Ok-Su;Kim, Young-Joon;Koh, Jung-Tae
    • Journal of Periodontal and Implant Science
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    • v.34 no.3
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    • pp.623-637
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    • 2004
  • 중증 만성 치주염과 1L-1B+3954 및 1L-1A+4845 유전자의 대립유전자 2 보유 유전자 다변성이 관련된다고 보고되었다. 그러나 이러한 1L-1 복합유전자 다변성과 만성 치주염 및 급진성 치주염과의 관련성에 대해서는 상반되게 보고되고 있는데 이는 인종적 배경과 질환특성의 차이에 기인한 것으로 보인다. 이 연구는 한국인에서 경도, 중등도와 중증의 만성 치주염 그리고 급진성 치주염 환자를 대상으로 하여 1L-1A+4845, 1L-1B+3954, 1L1B-511, 1L-1 RN intron 2 (VNTR) 유전자 다변성의 분포를 평가하고, 치주질환의 심도와 유형에 관련되는지 알아보고자 시행되었다. 전남대학교 병원 치주과에서 검진과 치료를 받은 100명의 치주질환자를 대상으로 하였고 질환군은 치주낭 깊이, 부착 소실, 골 소실을 기준으로 하여 경도, 중등도, 중증의 만성 치주염, 급진성 치주염군으로 분류하였다. 대조군으로는 전남대학교 병원 소아치과에 내원한 전신적으로 건강한 92명의 아동을 포함하였다. 각 대상 환자에서 채취된 협점막상피에서 genomic DNA를 얻어 1L-1A+4845, 1L-1B+3954, 1L-1B-511 genotype은 중합효소 연쇄반응을 시행한 후 제한 효소분해과정을 거쳐 전기영동 후 분리한 결과를 해석하였으며 1L-1 RN(VNTR) 유전형은 중합효소연쇄반응 후 분리한 결과를 해석하여 다음의 결과를 얻었다. 대립유전자 2 보유자 비율은 치주질환자에서 1L-1A+4845, 1L-1B+3954, 1L-1B-511, 1L-1 RN이 각각 61%, 13%, 76.6%, 34%였으며 대조군에서는 76.9%, 7.7%, 62.2%, 19.1%였다. 1L-1B+3954과 1L-1A+4845 대립유전자 2 보유자인 양성유전자형 비율은 경도, 중등도, 중증의 만성치주염, 급진성 치주염환자에서 각각 10%, 7.9%, 22.2%, 12% 였으며 치주질환자의 13%, 대조군의 7.7%에서 양성 복합유전자형(positive genotype)을 보였다. IL-1B-511 유전자 다변성은 치주질환자에서 대조군에 비하여 높았으며 급진성 치주염환자에서 대립유전자 2 보유자율이 유의하게 높았다(p<0.01). IL-1 RN intron 2 유전자 다변성은 중등도 및 중증 만성 치주염환자에서 대립유전자 2 보유자율이 유의하게 증가하였다. 이러한 결과는 IL-1 gene cluster의 유전형이 한국인에서도 치주염의 유형과 질환 심도에 관련될 수 있음을 시사하였다.

Full hereditary $C^{*}$-subalgebras of crossed products

  • Jeong, Ja A.
    • Bulletin of the Korean Mathematical Society
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    • v.30 no.2
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    • pp.193-199
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    • 1993
  • A hereditary $C^{*}$-subalgebra B of a $C^{*}$-algebra A is said to be full if B is not contained in any proper closed two-sided ideal in A, so each hereditary $C^{*}$-subalgebra of a simple $C^{*}$-algebra is always full. It is well known that every $C^{*}$-algebra is strong Morita equivalent to its full hereditary $C^{*}$-subalgebra, but the strong Morita equivalence of a $C^{*}$-algebra A and its hereditary $C^{*}$-subalgebra B does not imply the fullness of B, ingeneral. We present the following lemma for our computational convenience in the course of the proof of the main theorem. Note that $L_{B}$, $L_{B}$$^{*}$ and $L_{B}$ $L_{B}$$^{*}$ are all .alpha.-invariant whenever B is .alpha.-invariant under the action .alpha. of G.a. of G.a. of G.a. of G.f G.

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Preparation and Characterization of L-Arginine Containing Poloxamer Hydrogels for Solubilization and Sustained Release of Amphotericin B (Amphotericin B의 가용화 및 방출지속화를 위한 아르기닌 함유 폴록사머 하이드로젤의 제조 및 특성분석)

  • Shin, Baek-Ki;Baek, Eun-Jung;Kim, Ye-Tae;Jeong, Ji-Woong;Nho, Young-Chang;Lim, Youn-Mook;Park, Jong-Seok;Huh, Kang-Moo;Park, Jeong-Sook
    • Polymer(Korea)
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    • v.34 no.5
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    • pp.459-463
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    • 2010
  • Amphotericin B (AmB) is anti-fungal agent for the treatment of systemic fungal infections, but its poor solubility has limited clinical applications. In this study, a new gel formulation made up of L-arginine as solubilizer, thermosensitive Poloxamer 407 (P 407), and adhesive carbopol was designed for effective solubilization and delivery of AmB. The aqueous solubility of AmB was enhanced up to 2.6 mg/mL by addition of L-arginine. Aqueous P 407 solutions of more than 20% w/v showed thermo-induced sol-gel-sol phase transition. The phase transition behavior was affected by the presence of AmB and L-arginine, and the phase transition range was broadened by addition of carbopol. In vitro drug release was improved by the solubilizing effect of L-arginine, and the presence of mucoadhesive carbopol prolonged the release rate as a function of concentration.

Lp FOURIER-FEYNMAN TRANSFORMS AND CONVOLUTION

  • Ahn, Jae Moon
    • Korean Journal of Mathematics
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    • v.7 no.2
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    • pp.183-198
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    • 1999
  • Let $\mathcal{F}(B)$ be the Fresnel class on an abstract Wiener space (B, H, ${\omega}$) which consists of functionals F of the form : $$F(x)={\int}_H\;{\exp}\{i(h,x)^{\sim}\}df(h),\;x{\in}B$$ where $({\cdot}{\cdot})^{\sim}$ is a stochastic inner product between H and B, and $f$ is in $\mathcal{M}(H)$, the space of all complex-valued countably additive Borel measures on H. We introduce the concepts of an $L_p$ analytic Fourier-Feynman transform ($1{\leq}p{\leq}2$) and a convolution product on $\mathcal{F}(B)$ and verify the existence of the $L_p$ analytic Fourier-Feynman transforms for functionls in $\mathcal{F}(B)$. Moreover, we verify that the Fresnel class $\mathcal{F}(B)$ is closed under the $L_p$ analytic Fourier-Feynman transform and the convolution product, respectively. And we investigate some interesting properties for the $n$-repeated $L_p$ analytic Fourier-Feynman transform on $\mathcal{F}(B)$. Finally, we show that several results in [9] come from our results in Section 3.

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Design of a L-Type Aperture Coupled Circular Polarization Patch Antenna Using Microstrip Feeding (마이크로스트립 급전 L자형 개구면 결합 원편파 패치 안테나 설계)

  • 이종환;우종명
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.5
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    • pp.757-765
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    • 1999
  • In this paper, L-Type aperture coupled circular polarization patch antenna for mobile communication(IMT-2000, 1.9375 GHz), which is capable of being mounted in mobile unit, was designed and fabricated. This antenna is expanded to the $2\times2$ circular polarization array antenna for satellite mobile communications which are using the L-band frequency. The results are as followings : Returnloss 18.56 dB, beamwidth $60^{\circ}$, gain 7 dBd, axial ratio 0.9 dB in the case of single element and returnloss 28.43 dB, beamwidth $38^{\circ}$, gain 9.8dBd, axial ratio 1.5 dB in the case of $2\times2$ array, respectively.

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