• Journal of Life Science
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• v.17 no.6 s.86
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• pp.791-797
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• 2007

We studied the inhibitory effect of solvent fractions from doenjang on mutagenicity using Salmonella typhimurium TA100 in Ames test. We also investigated the effect of solvent fractions from doenjang on the growth of tumor cells and the production of interleukin-2 (IL-2). The treatment of dichlorormethane and ethylacetate fractions (2.5 mg/assay) from doenjang to Ames test system inhibited aflatoxin B$_1$ (AFB$_1$) induced mutagenicity by 96% and 97%, respectively, and showed a higher antimutagenic effect than other solvent fractions. In case of N-methyl-N'-nitro-N-nitrosoguamidine (MNNG) induced mutagenicity, the ethylacetate fraction showed the highest inhibitory effect (by 75%) among the other sol-vent fractions, although the inhibitory effect was not stronger compared to AFB$_1$ induced mutagenicity. The treatment of dichloromethane and ethylacetate fractions markedly inhibited the growth of Yac-1 (by 80% and 94%, respectively) and sacroma-180 cancer cells (by 60% and 96%, respectively) after 4 days of incubation at 37${\circ}$C. To elucidate the immunological mechanism of antitumor activity of doenjang, spleen cells of Balb/c mouse were exposed to the dichloromethane and ethyl-acetate fractions for 24 hours at 37${\circ}$C . The culture supernatants following the treatment of djchloromethane and ethylacetate factions to spleen cells increased the production of IL-2. These results indicated that the anticarcinogenic effect of doenjang was mediated by the production of IL-2.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1388-1394
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• 2012

The physiological functionalities of 70% ethanol extracts (EE) from Cudrania tricuspidata fruit (ECFD, EE of C. tricuspidata subjected to freeze-drying; ECHD, EE of C. tricuspidata subjected to heat air drying; ECID, EE of C. tricuspidata subjected to infrared drying) were investigated. Yields of freeze-dried powders of ECFD, ECHD, and ECID were 51.50%, 53.91%, and 56.14%, respectively. Color $L^*$, $a^*$, $b^*$, and $H^{\circ}$ values of ECHD and ECID decreased. Dried ECHD and ECID had relatively higher contents of total polyphenolics and flavonoids than ECFD. Electron donating abilities at a concentration of 10 mg/mL (w/v) were in order of ECID (62.37%) >ECHD (80.17%)>ECFD (77.80%). Reducing powers ($OD_{700}$) of ECFD, ECHD, and ECID were 0.75, 1.70, and 1.89, respectively. Additionally, ABTS radical scavenging ability of ECID was the highest with a value of 62.37% at a concentration of 10 mg/mL (w/v). Nitrite scavenging activities of ECFD, ECHD, and ECID at a concentration of 10 mg/mL (w/v) were 28.76%, 30.69%, and 41.64%, respectively. SOD (superoxide dismutase)-like activities at 5 mg/mL (w/v) were in the order ECFD (891.93 mUnits)>ECHD (723.02 mUnits)>EFID (611.97 mUnits). Whereas ferrous ion chelating activity of ECFD (52.36%) and ECID (47.16%) was significantly higher than that of ECHD (30.04%). ACE inhibitory and xanthine oxidase (XO) inhibitory activities of ECHD and ECID at a concentration of 1 mg/mL (w/v) were higher than those of ECFD. In conclusion, we provided experimental evidence that extracts of pre-dried C. tricuspidata exhibit increased physiological functionalities. Further, infrared drying technique is the best method for enhancement of antioxidant activity of C. tricuspidata fruit.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.101-108
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• 1999

This survey was performed from March 1993 to March 1998, in order to clarify the relationships between water quality and topographical factor. The study sites were two reservoir basins; Kaesim and Jangchan in Iwon-myon, Okchon-gun, Chungcho'ngbukdo Province. Basin shape factors of Kaesim reservoir were at 0.030∼0.210 (mean value 0.090), those of Jangchan reservoir were at 0.217∼0.452 (mean value 0.325). The mean basin shape factor of Jangchan reservoir was 3.61 times larger than that of Kaesim reservoir because its stream width was narrower and mean stream length was shorter. In the correlation between distance from the source of stream (L) and basin area (A), Iwonchon basin was calculated as L=1.44A/sup 0.6/. Circularity ratio was 17.114 in Kaesim (22% of Kum River), and 7.444 in Jangchan. Elongation ratio was 0.357 in Kaesim, 0.636 in Jangchan and 0.282 in Kum River. Precipitation summation period of Jangchan was 1.54 times slower than that of Kaesim. Rainfall reaching time in each small basin was 337.53 min. in A'(Jangchan-ri) basin of Jangchan and 49.26 min in H (Iwon-ri) basin of Kaesim. In the relationship between watershed frequency (Df) and drainage density (Dd), the regression equation was Df=0.023Dd² in Kaesim and Df=0.189Dd² in Jangchan reservoir. As slope degree increased, DO became higher (Y/sub DO/=0.19X＋6.5927, r=0.8l), but COD(Y/sub COD/=-0.2092X＋9.7104, r=0.52) became lower. Total nitrogen was increased with the increase of basin shape factor and circularity ratio. Ratio of B/sub OD/ to COD was 1/1.2(Y/sub BOD/ = 1.2984 X/sub COD/-3.2004, r=0.9l).