• Journal of the Korean Institute of Intelligent Systems
• /
• v.24 no.3
• /
• pp.251-258
• /
• 2014

The purpose of the neural image analysis is to trace the velocities and the directions of moving mitochondria migrating through axons. This paper proposes an automated technique for detecting axon structure. Previously, the detection process has been carried out using a partially automated technique combined with some human intervention. In our algorithm, a consolidated image is built by taking the maximum intensity value on the all image frames at each pixel Axon detection is performed through vessel enhancement filtering followed by a peak detection procedure. In order to remove errors contained in ridge points, a filtering process is devised using a local reliability measure. Experiments have been performed using real neural image sequences and ground truth data extracted manually. It has been turned out that the proposed algorithm results in high detection rate and precision.

• Biomolecules & Therapeutics
• /
• v.17 no.4
• /
• pp.362-369
• /
• 2009

We have investigated the effect of glucosamine on the retinal cells after continuous infusion into cerebroventricle by using osmotic minipump to avoid peripheral effect. Continuous intracerebroventricular (i.c.v) infusion of glucosamine with the rate of 0.1 ${\mu}mol$/10 ${\mu}l$/hr for 7 days resulted in morphological changes of the optic nerve in electron microscopic level as well as morphological changes of the retina in light microscopic level. Retinal sections were immunostained for the detection of morphological changes of astrocytes. GFAP immunoreactivity appeared not only in the Muller cells but also many of the radial processes of Muller cells. The optic nerve showed deformed axon and slight lamellar separation of myelin sheath after continuous infusion of glucosamine in observing with electron microscope. Interestingly, vacuoles were observed in deformed axons and retinal layers were folded and detached. These results suggested that glucosamine plays a role in induction of morphological dysfunction in retina and optic nerves.