• Title/Summary/Keyword: Avian bornavirus

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First detection of avian bornavirus by RT-PCR in proventricular dilatation disease-suspected Hahns Macaw (Ara nobilis nobilis) in Korea (선위확장증-의심 한스 마코앵무새(Ara nobilis nobilis )로부터 RT-PCR에 의한 avian bornavirus 최초 검출)

  • Kim, Jin-Hyun;Lee, Bu-Heung;Cho, Jae-Keun;Yoon, Won-Koung;Kim, Won;Kim, Hee-Jung;Kim, Eun-Mi;Kim, Ki-Seuk;Park, Choi-Kyu
    • Korean Journal of Veterinary Service
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    • v.37 no.1
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    • pp.79-84
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    • 2014
  • Two Hahns Macaws (Ara nobilis nobilis), three and nine-month-old, with a three-weeks history of weight loss and anorexia were diagnosed presumptively with proventricular dilatation disease (PDD) by radiographic examination. The birds were treated with antimicrobials, analgesics, and fluid administration. However, these birds died three weeks after the first signs of PDD. At necropsy, the birds had severely dilated proventriculus, severe pectoral muscles atrophy, and blood vessels congestion on cortex of cerebrum. The partial matrix gene of avian bornavirus (ABV) was detected by RT-PCR from tissues of the brain, feather calami, and proventiculus of each PDD-suspected birds. This report describes the first detection of ABV in PDD-suspected Hahns Macaw in Korea.

First detection of avian polyomavirus by PCR from Alexandrine Parakeet (Psittacula eupatria) in Korea (대본청 앵무(Psittacula eupatria )로부터 PCR에 의한 avian polyomavirus 최초 검출)

  • Kim, Hee-Jung;Lee, Sun-Rock;Park, Choi-Kyu
    • Korean Journal of Veterinary Service
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    • v.37 no.3
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    • pp.213-218
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    • 2014
  • In early April 2014, a month-old Alexandrine Paraqeet (Psittacula eupatria) that was raised in a domestic aviary located in Gyungju-si, Korea was suddenly died and submitted to Animal Disease Intervention Center, Kyungpook National University in order to diagnose the causative agent. In post-mortem examination, the bird had abnormally developed feathers on the neck and abdomen region and subcutaneous hemorrhages on the neck and cheek adjacent to the beak. At necropsy, the bird had hemorrhage on the muscle of the femoral region, ascites, multi-focal hemorrhages on the epicardium, and diffuse hemorrhages on the sub-serosa of proventriculus and gizzard, suggesting typical avian polyomavirus (APV) infection. The partial large tumor (T) antigen gene of APV was detected by PCR from tissues of the heart, lung, liver, kidney, proventriculus and feathers of the APV-suspected birds. However, other pathogenic virus-specific nucleic acid common with psittacine birds such as avian bornavirus, psittacine beak and feather disease virus and psittacid herpesvirus were not detected from the mixed tissue samples of the bird, indicating this case is due to single infection of APV. Nucleotide sequence analysis of the partially amplified large T antigen DNA was confirmed to have 99~100% homology with that of the previously reported APV strains. This case report describes the first detection of APV in Alexandrine Paraqeet in Korea.

Detection of psittacine beak and feather disease virus from a caged blue and yellow macaw (Ara ararauna) in Korea (국내 청금강 앵무새(Ara ararauna )에서 psittacine beak and feather disease virus 최초 검출)

  • Kim, Hee-Jung;Kang, Dae-Young;Kim, Eun-Mi;Kim, Eun-Gik;Lee, Bu-Heung;Yeo, Sang-Geon;Park, Choi-Kyu
    • Korean Journal of Veterinary Service
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    • v.37 no.3
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    • pp.219-224
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    • 2014
  • A eight-month-old blue and yellow macaw (Ara ararauna) with psittacine beak and feather disease (PBFD)-suspected signs, such as, abnormal feather, depression and diarrhea, was presented to Animal Disease Intervention Center, Kyungpook National University in 16 April 2014. The partial ORF V1 gene of PBFD virus (PBFDV) was detected by polymerase chain reaction (PCR) from DNA templates extracted from feather, blood and cloacal swab sample of the bird, but no other viral DNAs that often infected in psittacine birds including avian bornavirus and avian polyomavirus were detected from the samples of the bird, indicating this case is due to single infection of PBFDV. Nucleotide sequence analysis of the amplified partial ORF V1 gene was confirmed to have 96.7% and 93.6% homology with that of previously reported PBFDV strain (Genbank no. HM748924 and FJ685980). This report describes the first detection of PBFDV in PBFD-suspected blue and yellow macaw in Korea.