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Correlation between Fluoromicroscipic Assessment of Mitochondria Function of Frozen-Thawed Hanwoo Spermatozoa and Blastocyst Development Following In Vitro Fertilization

  • Park Sae-Young;Kim Eun-Young;Kim Deok-Im;Lee Won-Don;Park Se-Pill;Lim Jin-Ho
    • Reproductive and Developmental Biology
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    • v.29 no.3
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    • pp.141-144
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    • 2005
  • This experiment was to investigate whether the mitochondria function assessment can be used for the prediction of sperm fertility through examining the correlation between mitochondria fluoromicroscopic frequency of frozen-thawed eight Hanwoo bull semen using rhodamine123 (R123) and in vitro embryo development following fertilization. Individual sperm were stained in 5 ${\mu}g/mL$ R123-added calcium-free Sp-TALP for 30 min at 0 h, 6 h, 12 h and 24 h after thawing and examined their mid-piece under an epifluorescence microscope using 495 nm excitation filter (x1,000). Three replications were taken, and at least 300 sperm per individual were examined. When semen samples were separated into two groups (good and poor) by sperm motility and fluorescent frequencies at just after thawing, average fluorescent frequencies were remarkably reduced as time going (0 h; $53.29{\~}72.94\%$, 6 h; $21.40{\~}58.90\%$, 12 h; $8.26{\~}25.93\%$, 24 h; $1.00{\~}13.78\%$, irrespective of selected group, and there were no differences at 6 h or 12 h after thawing between selected groups but indicated significant difference at 24 h after thawing (p<0.05). In vitro fertilization rates in good and poor groups ranging $70.8{\~}77.8\%$ and $52.1{\~}84.5\%$, respectively, were not significantly different. However, in vitro development rates of the same groups ranging $25.7{\~}40.0\%$ and $12.9{\~}1.8\%$, respectively, were significant different (p<0.05). These results demonstrate that mitochondria fluoromicroscopic assessment of frozen-thawed bovine sperm may be used as a criterion to select more fertile sperm.

Discrimination of Local Microearthquakes and Artificial Underground Explosions on the Basis of Time-Frequency Domain (시간-주파수 영역에서의 국지 미소지진과 지하인공폭발의 구별)

  • 김소구;박용철
    • The Journal of Engineering Geology
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    • v.7 no.1
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    • pp.63-79
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    • 1997
  • In this study, our purpose is to develop a technique to discriminate artificial explosions from local microearthquakes on the basis of time-frequency domain. To obtain spectral features of artificial explosions and microearthquakes, we used 3-d spectrograms(frequency, time and amplitude) because this is a useful tool to study the frequency content of entire seismic waveforms observed at local and regional distances (e. g., Kim et al., 1994). P and S waves from quarry blasts show that frequency content of dominant amplitude appeared above 10 Hz and Rg phases that are observed at near distance ranges. But P and S waves from microearthquakes have more broad frequency content as well as below 10 Hz. And for discrimination, Pg/Lg spectral ratio is performed below 10 Hz. In order to select time windows we computed group velocity using multiple filter method(MFM) and removed free surface effects from all 3-components data for improving on data quality. Next step, we computed Fast-Fourier transform, and a log average spectral amplitude over seven frequency bands : 0.5 to 3, 2 to 4, 3 to 5, 4 to 6, 5 to 7, 6 to 8 and 8 to 10 Hz. The best separation is observed from 6 to 8 Hz.

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Analysis of Elevation NDVI (Normalized Difference Vegetation Index) for Taxus cuspidata, Pinus densiflora, Zelkova serrata and Acer palmatum - Focused on landscaping trees in Kangwon National University - (소나무, 주목, 느티나무 그리고 단풍나무의 입면 NDVI 비교 분석 - 강원대학교 내 조경수목식재종을 대상으로 -)

  • Kil, Sung-Ho
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.20 no.6
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    • pp.151-160
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    • 2017
  • This study was conducted by using a Nikon Coolpix S800c camera equipped with a NIR filter to measure the NDVI(Normalized Difference Vegetation Index). It was used for the measurement of the three trees of Pinus densiflora, Taxus cuspidata, Zelkova serrata and Acer palmatum in Kangwon National University. The NDVI value of the surface of the building was compared and analyzed. The average value of NDVI in August and September was high in all species. The NDVI distribution of Taxus cuspidata was higher than the other trees. The NDVI distribution of Pinus densiflora and Taxus cuspidata did not show any significant seasonal differences, but The NDVI distribution of Zelkova serrata and Acer palmatum were relatively low in May and June, which are leafless periods. Previous studies related to NDVI value were generally analyzed using satellite imagery. However, it was scarce related to study the NDVI value of each tree or study the changing process of NDVI by time series. Previous studies have used NDVI values on the ground but this study used NDVI values in the ground section. Future studies will be necessary to measure the NDVI value at different times for various species and also to make efforts to generalize the measurement method. In addition, research related to various fields such as the relationship between NDVI and carbon stocks and the relationship with LAI needs to be conducted.

Studies on the Chemical Compositions and Distributions of Ambient Sumicron Aerosols (Submicron 부유분진의 화학적 조성 및 분포에 관한 연구)

  • 황인조;김동술
    • Journal of Korean Society for Atmospheric Environment
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    • v.14 no.1
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    • pp.11-23
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    • 1998
  • The purpose of this study was to survey chemical distribution of inorganic elements and ions in the submicron particles, to characterize qualitatively emitting sources by factor analysis, and finally to reveal existing patterns in terms of chemical compounds by a stepwise multiple regression analysis. Total of 141 samples were collected by a cascade impactor from 1989 to1996. Fifteen chemical species (Al, Ba, Cd, K, Pb, Cu, Fe, Ni, $Cl^-, NO_3^-, SO_4^{2-}, K^+, Mg^{2+}, Ca^{2+}, and Na^+$) were characterized by AAS and IC. The study showed that average seasonal levels of submicron particulate matters $(d_p<0.43 \mum)$ were 18.7 $\mug/m^3$ in spring, 15.5 $\mug/m^3$ in summer, 15.7 $\mug/m^3$ in fall, and 24.5 $\mug/m^3$ in winter, respectively. All of the anion concentrations in the particle were highest in the winter season. By applying a factor analysis, 5 source patterns were qualitatively obtained, such as sulfate related source, nitrate related source, oil burning source, calcium related source, and coal combustion source. Finally, when applying a stepwise multiple regression analysis, the results clearly showed that $Na^+ and Ca^{2+}, K^+ and Ca^{2+}, NO_3^-$ and relative humidity, $Cl^-$ and ambient temperature, $Ca^{2+} and Cl^-, Mg^{2+} and SO_4^{2-}, Na^+ and NO_3^-, and Ca^{2+} and NO_3^-$, respectively, are negatively contributed to each other. As a result of those statistical analysis, we could suggest that some chemical compounds in the submicron particles such as$NaNO_3, MgSO_4, Ca(NO_3)_2, and CaCl_2$ may not exist on the filter as final composing products; however, other compounds may possibly exist in the form of $Mg(NO_3)_2, CaSO_4, Na_2SO_4, K_2SO_4, MgCl_2, NaCl, and KCl$. Thus, it must be necessary to identify differences between the results of above statistical analysis and of the real world by laboratory experiments.

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Experimental investigation of the effect of baffles on the efficiency improvement of irrigation sedimentation tank structures

  • Nouri, Alireza Zamani;Heydari, Mohammad Mehdi
    • Structural Engineering and Mechanics
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    • v.63 no.4
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    • pp.567-574
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    • 2017
  • Sedimentation tanks are essential structures to filter the suspended sediments in the inlet flow which are constructed at the inlet of the basins forked from rivers and irrigation canals. The larger the constructed tank, the better the sedimentation process is conducted. However, the construction and dredging costs increase. In this regard, improving the performance and sedimentation efficiency seem necessary by alternative methods. One of these effective methods is using baffle plates. Most of the studies carried out in this field are on the use of these baffles in the primary and secondary sedimentation tanks. Hence, this study is carrier out with the objective of increasing the retention efficiency in the irrigation sedimentation tanks using baffles. To reach this goal, the experiments were carried out in a flume with length 8 meters, width 0.3 meters, and height 0.5 meters, considering a sedimentation tanks with a length of 3 meters, in three different inlet concentration, three flow rates and three Froude numbers. The baffles were mounted at the bottom of the tank and the effects of the angle, height and position in the tanks were investigated. The results showed that on average, employing the baffles increased the sedimentation efficiency 5 to 6% and the highest value was obtained for angle 60 with respect to the flow direction. According to the results of this study, the most favorable height and position of these baffles were obtained to be in 40% of the depth of the flow and 50% of the length of the sedimentation tank, respectively. Also, by increasing the number of baffles, the sedimentation efficiency decreased. Regarding the sedimentation regions in this case, more than 80% of the settled sediments were observed in the middle of the tank measured from the inlet.

Effects of Crustal Species on Characteristics of Aerosols: Simulation of Measurements at Kosan, Cheju Island, 1994 (토양 성분이 입자 특성에 미치는 영향: 제주도 고산에서의 1994년 측정결과 모사)

  • 김용표;문길주
    • Journal of Korean Society for Atmospheric Environment
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    • v.12 no.3
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    • pp.289-296
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    • 1996
  • Effects of crustal species on the characteristics of ambient particles were studied by applying a gas-particle equilibrium model, SCAPE, to the measurements at Kosan, Cheju Island during the spring and summer, 1994. Two cases were simulated; the measured composition was used without any modification (case 1), and the metal ion concentrations originated from crust were subtracted from the measured particle composition (case 2). Total suspended particles (TSPs) were collected by an automatic high volume tape sampler during spring period and by high volume samplers during summer period. The fine particles, PM 2.5, and gaseous volatile species were collected using a filter pack smapler during summer period. The water soluble ion concentrations were analyzed from all the particle samples. According to the simulation results, the effect of crustal elements on the chemical composition of particles is negligible for both TSP particles and PM 2.5 particles. Acidity of particles measured at Kosan, however, is affected by the change of the concentrations of crustal species, stronger effects for TSP particles than for PM 2.5 particles during summer, and stronger effects during summer than spring for TSP particle. The average pH decrease due to the absence of crustal species was about 0.10 for PM 2.5 particle during summer and 1.51 and 0.85 for TSP particles during summer spring, respectively. Water contents of PM 2.5 particles for both cases are comparable to each other. Estimated water content of TSP particles for case 2 is higher than that for case 1 by about 4 $\sim 6 \mum/m^3$ because salts of metal ions are not hygroscopic.

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Edge Enhanced Halftoning using Spatial Perceptual Properties of Human (인간의 공간 지각 특성을 이용한 에지 강조 컬러 해프토닝)

  • Kwak Nae-Joung;Chang Un-Dong;Song Young-Jun;Kim Dong-Woo
    • The Journal of the Korea Contents Association
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    • v.5 no.3
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    • pp.123-131
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    • 2005
  • Among the digital halftoning, the error diffusion halftoning gives better subjective quality than other halftoning techniques. But it also makes edges of objects blurred. To overcome the defect, this paper proposes the modified error diffusion halftoning algorithm to enhance the edges using the spatial perceptual properties of the human visual system. Using the properties that the human eyes perceive not the pixel's luminance itself but the local average luminance and the information that human eyes perceive spatial variation, the proposed method computes information of edge enhancement(IEE). The IEE is added to the quantizer's input pixel and feeds into the halftoning quantizer. The quantizer produces the halftone image having the enhanced edge. Also this paper proposes the technique that the coefficients of the error diffusion filter are adapted according to the correlation among color components. The computer simulation results show that the proposed method produces finer halftoning images than conventional methods due to the enhanced edges. And the proposed method also preserves similar in edges to original image and reduces some defects such as color impulse and false contours.

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Navel Area Detection Based on Body Structure (신체의 구조를 기반으로 하는 배꼽 영역 검출)

  • Jang, Seok-Woo;Huh, Moon-Haeng
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.3
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    • pp.2185-2191
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    • 2015
  • With the advance of the environment where we can get various multimedia contents, adult image detection has become an important issue these days. In this paper, we suggest a method of robustly detecting navel areas from input images which can be usefully utilized in adult image detection. The suggested algorithm first extracts face regions and extracts candidate nipple areas using a nipple map. Our method then selects only actual nipple regions by filtering candidate areas with geometrical features and an average nipple filter. Subsequently, the method robustly detects navel areas by using the structural relation with the nipple areas and applying edge and saturation images. Experimental results show that the suggested algorithm can effectively detect navel regions.

Characteristics of Ionic Components in Size-resolved Particulate Matters in Suwon Area (수원지역 분진의 입경별 이온성분 분포특성에 관한 연구)

  • Oh, Mi-Seok;Lee, Tae-Jung;Kim, Dong-Sool
    • Journal of Korean Society for Atmospheric Environment
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    • v.25 no.1
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    • pp.46-56
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    • 2009
  • The main purpose of this study was to investigate air quality trends of ambient aerosol with obtaining size-fractionated information. The suspended particulate matters were continuously collected on membrane filters and glass fiber filters by an 8-stage cascade impactor for 2 years (Sep. 2005 $\sim$ Sep. 2007) in Kyung Hee University-Global Campus. 8 ionic species ($Na^+$, ${NH_4}^+$, $K^+$, $Mg^{2+}$, $Ca^{2+}$, $Cl^-$, ${NO_3}^-$, and ${SO_4}^{2-}$) were analyzed by an IC after performing proper pretreatments of each sample filter. The average concentration levels of each ion were $9.24{\mu}g/m^3$ of ${SO_4}^{2-}$, $7.35{\mu}g/m^3$ of ${NO_3}^-$, $2.81{\mu}g/m^3$ of ${NH_4}^+$, $2.11{\mu}g/m^3$ of $Ca^{2+}$, $1.65{\mu}g/m^3$ of $Cl^-$, $1.87{\mu}g/m^3$ of $Na^+$, $0.80{\mu}g/m^3$ of $Mg^{2+}$, and $0.54{\mu}g/m^3$ of $K^+$, respectively. The distribution pattern of $Na^+$, $Mg^{2+}$, $Ca^{2+}$, $Cl^-$, and ${NO_3}^-$ was bi-modal and two peaks appeared in the range of $0.4{\sim}0.7{\mu}m$ and $3.3{\sim}4.7{\mu}m$, respectively. On the other hand, ${SO_4}^{2-}$, ${NH_4}^+$, and $K^+$ showed patterns of uni-modal distribution, mostly abounded in the fine mode group.

Design, fabrication, and evaluation of RF module in compliance with the IEEE 802.11a standard for 5GHz-band Wireless-LAN applications (IEEE 802.11a 규격을 만족하는 5GHz 대역 무선 랜용 RF 모듈의 설계, 제작과 성능 평가)

  • 권도훈;김영일;이성수;박현철
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.3C
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    • pp.248-255
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    • 2002
  • An RF module in compliance with the IEEE 802.11a standard has been designed and its performance has been measured. Conventional heterodyne architecture with 580MHz intermediate frequency has been realized. Measurement results show that the receiver has a low Noise Figure of 5dB, the maximum gain of 70dB, and dynamic range as wide as 61dB. Also, the SAW filter used for channel selection in the IF section allowed minimum inter-channel interference. in addition to satisfying the RF output power requirement, the transmitter features its output P1dB as high as 34dBm so that the high peak-to-average ratio of the Orthogonal Frequency Division Multiplexing (OFDM) modulation scheme can be handled with minimum nonlinear distortion. The output P1dB of 34dBm of the transmitter corresponds to back-off powers of 18dB and 11dB with respect to the output power for the low and the middle frequency bands, respectively.