• Toxicological Research
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• v.33 no.4
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• pp.315-323
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• 2017

Preconceptual sex selection is still a highly debatable process whereby X- and Y-chromosome-bearing spermatozoa are isolated prior to fertilization of the oocyte. Although various separation techniques are available, none can guarantee 100% accuracy. The aim of this study was to separate X- and Y-chromosome-bearing spermatozoa using methods based on the viability difference between the X- and Y-chromosome-bearing spermatozoa. A total of 18 experimental semen samples were used, written consent was obtained from all donors and results were analysed in a blinded fashion. Spermatozoa were exposed to different pH values (5.5, 6.5, 7.5, 8.5, and 9.5), increased temperatures ($37^{\circ}C$, $41^{\circ}C$, and $45^{\circ}C$) and ROS level ($50{\mu}M$, $750{\mu}M$, and $1,000{\mu}M$). The live and dead cell separation was done through a modified swim-up technique. Changes in the sex-chromosome ratio of samples were established by double-label fluorescent in situ hybridization (FISH) before and after processing. The results indicated successful enrichment of X-chromosome-bearing spermatozoa upon incubation in acidic media, increased temperatures, and elevated $H_2O_2$. This study demonstrated the potential role for exploring the physiological differences between X-and Y-chromosome-bearing spermatozoa in the development of preconceptual gender selection.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.4
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• pp.265-270
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• 1989

Soil properties for the limestone-derived soil were examined to relate soil formation to stratigraphy of parent materials and hillslope positions in Bangjeol Ri, Yeongweol Eup, Gangweon Do. Pyeongchang, Anmi, Yulgog and Mungyeong series were described for topographic positions such as shoulder position, footslope, terrace and local bottom associated with toposequence where the landscape consisted of gently rolling hillslopes and nearly level plains. 1. Pyeongchang, Anmi, Yulgog and Mungyeong series had the standard hue of the 2.5YR, 5YR, 10YR and 2.5Y, respectively. Thus, color sequence of soil could be related to hillslope positions on the landscape. 2. With ascending slope toward summit, the clay content increased while silt content decreased 3. Silt/Clay ratios ranged from 0.27 to 3.76 and it was increased with descending to bottom. It, also, appeared that maturity of soil was higher at summit position than at bottom. 4. Soils developed in limestone were neutral in soil reaction and very low in available $P_2O_5$. OM, available $SiO_2$, CEC, and active Fe in soils seemed to be increased with ascending to summit position.

• Bulletin of the Korean Chemical Society
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• v.16 no.7
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• pp.634-641
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• 1995

The metal-carbon σ-bond cluster complexes 1-Mn(CO)5-2-R-1,2-C2B10H10 (R=CH3 Ia, C6H5 Ib) have been prepared in good yields from readily available carboranyl lithium complexes, 1-Li+-2-R-1,2-C2B10H10- (R=CH3, C6H5), by direct reaction with (CO)5MnBr. These manganese metal complexes are rapidly converted to the corresponding manganese metal carbene complexes, 1-[(CO)4Mn=C(OCH3)(CH3)]-2-R-1,2-C2B10H10 (R=CH3 IIIa, C6H5 IIIb), via alkylation with methyllithium followed by O-methylation with CF3SO3CH3. The crystal structure of IIIb was determined by X-ray diffraction. Thus, complex IIIb crystallizes in the orthorhombic space group P212121 with cell parameters a=15.5537(5), b=19.0697(5), c=7.4286(3) Å, V=2203.4(1) Å3, and Z=4. Of the reflections measured a total of 3805 unique reflections with F2>3σ(F2) was used during subsequent structure refinement. Refinement converged to R1=0.053 and R2=0.091. Structural studies showed that the manganese atom had a slightly distorted pseudo-octahedral configuration about the metal center with the carbene and ortho-carborane occupying the equatorial plane cis-orientation to each other.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.123-131
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• 1999

To get the basic information for the improvement of the optimum levels of upland soil fertility and fertilizer application, the soil samples in two hundred fifteen site were collected from the major condiment vegetable cultivation areas such as red pepper, garlic and onion fields. Physico-chemical properties of the soil samples were investigated. Soil texture distribution of soil samples in red pepper, garlic and onion cultivation areas was in order of loam (L), sandy loam (SL), silt loam (SiL) and clay loam (CL) (35.4, 31.6, 14.9 and 7.0%, respectively). The average pH of soil in garlic and onion cultivation areas were over pH 6.0, whereas in red pepper was under pH 5.5. The frequency distribution of soil pH in total sampling sites were 58.7% in under pH 6.0 and 21.4% in below pH 5.0, in contrast to 10.3% in over pH 7.0. The organic matter contents were in the range of $20{\sim}30g\;kg^{-1}$, and the onion field soils was a little higher than those in red pepper or garlic. The available phosphate contents were in the range of $719{\sim}746mg\;kg^{-1}$ and were not different among in red pepper, garlic and onion. The frequency distribution of available phosphate in total sampling sites were found 62.8% of above $600mg\;kg^{-1}$, which was over the standard level for upland soil improvement, and then 22.3% was exceeded $1,000mg\;kg^{-1}$, especially. In the exchangeable cations, the K and Ca contents in garlic (1.27 and $9.11cmol\;kg^{-1}$) and onion (1.20 and $8.39cmol\;kg^{-1}$) were higher than in red pepper (0.96 and $5.87cmol\;kg^{-1}$), respectively. The Mg contents in garlic field soils ($2.17cmol\;kg^{-1}$) were higher than those in red pepper and onion (1.51 and $1.80cmol\;kg^{-1}$). This exchangeable K, Ca and Mg contents were higher than the standard level for upland soil improvement. The contents of microelement were ranged in $54.3{\sim}60.1mg\;kg^{-1}$ for Fe, $31.3{\sim}42.3mg\;kg^{-1}$ for Mn, $1.7{\sim}2.3mg\;kg^{-1}$ for Cu and $4.8{\sim}5.5mg\;kg^{-1}$ for Zn, respectively.

• Korean Journal of Organic Agriculture
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• v.5 no.1
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• pp.125-135
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• 1996

A study was made to investigate the possibility of treating various organic waste of cattle manure, swine manure, goat manure, apple pomace, chinese medicine residue and coffee less by feeding these to earthworms and then by studying the growth and reproductive efficiency of earthworms, and the chemical composition of worm cast and its production. The results are summarized as follows. 1. When the feed for earthworms were in good condition which were cattle manure, swine manure and apple pomace, the reproductive efficiency of earthworms was improved however in worse condition the feed of which were goat manure and chinese medicine residue, the increasing rate(IR) became faster. 2. Despite the high content of organic matter(OM) in coffee lees, the earthworms fed coffee lees showed significantly lower reproductive efficiency and increasing rate(IR) than those fed other organic waste due mainly to its lower pH and lower total nitrogen(TN) content. Therefore, when coffee lees is considered to be fed to earthworms, it is believed necessary to be mixed with addiveves or other organic waste in order to improve the feed condition. 3. pH in swine manure, goat manure, apple pomace, chinese medicine residue and coffee lees became neutralized by being fed by earthworms. 4. Available P2O5 and exchangeable cation(EC) of earthworm cast were a little increased compared to those of feed. The contents of Mn, Fe and Cu in earthworm cast were a little decreased to compared to those of feed. 5. The contents of Mn, Fe and Cu in earthworm cast were a little a decreased to compared to those of feed. 6. Mean fresh weight of earthworm at final time(FW2) was negatively correlated with number of young worms(NY)(P<0.01), but positively with increasing rate(IR) (P<0.001) and C/N ratio(P<0.05) respectively. Number of cocoons (NC) and fresh weight of cocoons(WC) were positively(P<0.01) correlated with fresh weight of young worms (WY) and digested matter(DI) but negatively(P<0.001) correlated with residual matter(WY) and digested matter(DI) but negatively(P<0.001) correlated with residual matter(RW). Number of young worms(NY) and fresh weigh of young worms(WY) were negatively(P<0.001) correlated with increasing rate(IR), however increasing rate(IR) of earthworm was positively(P<0.05) correlatedd with C/N ratio.

• Current Research on Agriculture and Life Sciences
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• v.25
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• pp.1-5
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• 2007

This study was carried out to offer the raw data of floricultural resource by scientific analyzing the growth and soil environment in Viola orientalis habitat, and establish the ecological characteristics in developing the relative model. In the results of soil factors analysis, there showed that acidity was pH 5.1, organic matter content of 9.1%, available $P_2O_5$ of 40.6mg/kg, exchangeable $K^+$ of $0.2cmol^+/kg$, exchangeable $Ca^{2+}$ of $3.5cmol^+/kg$, exchangeable $Mg^{2+}$ of $0.8cmol^+/kg$, cation exchange capacity(C.E.C) of $13.7cmol^+/kg$ and electrical conductivity(EC) of 0.4dS/m. The growth characteristics were surveyed that height was 12.4cm, leaf width of 2.5cm, leaf length of 3.0cm, flower width of 2.5cm, peduncle of 2.3cm and chlorophyll of $38.5{\mu}g{\cdot}mg^{-1}$.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.6
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• pp.357-365
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• 2006

The investigations were conducted to find out the situation of fertilizer use and the contents of soil chemical components on summer vegetable crops at 791 farmers' upland fields located in the parts of Gangwon-do, Gyengsangbuk-do and Jeollabuk-do of alpine area. Major vegetable crops were potato, Chinese cabbage, radish, carrot, onion, and cabbage. From the location surroundings cultivated alpine vegetable crops, the orders were Gangwon-do>Gyeongsangbuk-do>Jeollabuk-do part in the sizes of a fie1d area and the height above sea level, and Jeollabuk-do>Gyeongsangbuk-do>Gangwon-do part in the slope degrees. The soil texture was of wide distribution on sandy loam soil for Gangwon-do(76%) and Jeollabuk-do part(64%), and 1oam(42%) and sandy loam soil(35%) for Gyeongsangbuk-do part. From the numbers of investigated fields, the distribution of slope degree was wider than those of height above sea level in relation to location surroundings. The upland soils of 785 fields cultivated vegetable crops were sampled at 0~15 cm of top soil before seeding or transplanting and analyzed. On an average, pH, organic matter, available phosphate and exchangeable potassium, calcium, magnesium of soil were 5.7, $27.6g\;kg^{-1}$, $765mg\;kg^{-1}$, $1.16cmol_c\;kg^{-1}$, $6.1cmol_c\;kg^{-1}$, and $1.6cmol_c\;kg^{-1}$, respectively. The average cation exchange capacity(CEC) of 120 sites in Gangwon-do part was $9.2cmol_c\;kg^{-1}$. The content of organic matter, exchangeable potassium, exchangeable calcium and exchangeable magnesium was higher, while that of available phosphate was lower with slope degrees. And the content of major chemical components in carrot soil was lower in comparison with other crop soils. The average levels of N, $P_2O_5$, $K_2O$, livestock manure and lime fertilizer of 785 Belds applied by farmers were 335, 198, 244, 12,680 and $1,750kg\;ha^{-1}$, respectively, for summer vegetable crops in alpine area. The average amounts of $N-P_2O_5-K_2O$ fertilizers applied by farmers in 785 Gelds of vegetable crops were higher 1.7~2.0-4.2~7.0-1.4~2.0 times on potato, 1.4~1.6-4.6~8.3-3.5~4.2 times on Chinese cabbage, and 1.2~1.3-4.2~7.2-3.0~3.61 times on radish than the rates of NPK fertilizers based on soil testing for each crop.

• Environmental Engineering Research
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• v.19 no.3
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• pp.241-245
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• 2014

The As accumulation in part of roots, shoots, husks and grains of rice plants was significantly decreased with the increasing dosage of kaolin addition from 0.5% to 10% w/w. Kaolin addition could reduce As accumulation in rice plants, which mainly could be attributed to the formation of stable crystalline Al oxides bound As that decreased the available As in soil with decreased As accumulation in rice plants. The pH values of the soils did not change significantly when amended with kaolin. The pH values of the soils was neural that proper to adsorb of arsenic with $Al_2O_3$. Arsenic tends to adsorb with $Al_2O_3$ at acid neutral pH and with desorbing at alkaline pH. The dry weight of rice plant was significantly increased with the increasing dosage of kaolin addition from 2.5% to 10% w/w. The highest dry weight of rice plants was 6.67 g/pot achieved at kaolin addition of 10% w/w with about 13% increasing over the control, which was probably attributed to the highest As concentration formation with kaolin at this dosage. The results of this study indicated that kaolin has the potential to reduce As accumulation in rice plants and enhance the dry weight of rice plants.

• Bulletin of the Korean Chemical Society
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• v.30 no.1
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• pp.172-176
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• 2009

The nature and morphology of titanium dioxide films play a significant role in determining the overall efficiency of dye-sensitized solar cell (DSSCs). In this work, the preparation of nanostructured titania particles by sol-gel method (SG-$TiO_2$) and its characterization were investigated for the application of DSSCs. The samples were characterized by XRD, XPS, FE-SEM, BET and FT-IR analysis. The energy conversion efficiency of SG-$TiO_2$ was approximately 8.3 % under illumination with AM 1.5 (100 mW/$cm^2$) simulated sunlight. DSSCs made of SG-$TiO_2$ nanocrystalline films as photoanodes achieved better energy conversion efficiency compared to those prepared using commercially available Degussa P25.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.273-280
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• 2019

BACKGROUND: The reclaimed lands for agricultural use managed by the Korean government is consisted of 17,145 hectares of lands under construction and 13,384 hectares of completed lands. In order to utilize these reclaimed lands as competitive agricultural complexes, the government is preparing to develop comprehensive development plans for multiple purposes. For rational land-use planning and soil management, information of the soil chemical properties is necessary. METHODS AND RESULTS: From 2013 to 2016, soil samples were collected from 85 representative sampling sites of the reclaimed lands and analyzed for soil chemical properties including electric conductivity (EC), pH, soil organic matter (SOM), and nutrients. The annual mean soil EC ranged from 5.1 to 8.3 dS m^-1 and have continued to decrease over the years (estimation equation with EC as dependent and year as independent variable was y =0.0736x² - 1.4985x + 9.8305, R² = 0.9753). The pH ranged from 7.3 to 7.6, which was higher than the optimum range (5.5~7.0) for agricultural soils. Soil organic matter (8 to 11 g kg^-1) was lower level than the optimum range (20~30 kg^-1). Available silicate (Av.SiO₂) ranged from 169 to 229 mg kg^-1, which was close to the minimum content (≥157 mg kg^-1) for rice paddy field. Available phosphate (Av.P₂O₅) content (24~39 mg kg^-1) was lower than the optimum range (80~120 mg kg^-1) for rice paddy field. CONCLUSION: For efficient agricultural use of reclaimed lands under government management, our results suggest that the application of organic matter and supplying deficient nutrients as well as desalinization is required.