• Title/Summary/Keyword: Auxins

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Production of Auxins and Auxin-like Compounds by Ginseng Growth-promoting Bacterium Pseudomonas fluorescens KGPP 207

  • Ten, Leonid N.;Lee, Mi Ja;Lee, Mee-Kyoung;Park, Hoon;Yoon, Jong Hyuk
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.264-268
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    • 2000
  • High activity of acidic ethylacetate extract from the culture supernatant of ginseng growth-promoting bacterium Pseudomonas fluorescens KGPP 207 and its fractions were demonstrated through wheat coleoptile bioassay. The following auxins and auxin-like compounds were identified in these fractions by combined gas chromatography-mass spectrometry: indole-3-acetic acid, indole-3-acetic acid methyl and ethyl ester, indole-3-butyric acid, indole-3-lactic acid and its methyl ester, indole-3-propionic acid, indole-3-pyruvic acid, p-hydroxyphenyl acetic acid, p-hydroxyphenyl acetic acid methyl and ethyl ester, phenyl acetic acid and its methyl ester. The bacterium KGPP 207 belongs to the strain of P. fluorescens which produces plant growth regulators and its beneficial effect on the ginseng growth may be due to the formation of the identified compounds.

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Specificity of Auxin Action on Ethylene Production in Corn Coleoptile Segments (옥수수(Zea mays L.) 자엽초 절편에서 에틸렌 생성에 대한 오옥신의 작용 특성)

  • 윤인선
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.325-330
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    • 1991
  • The ability of several auxin analogs to induce ethylene production was tested in the corn coleoptile. The synthetic auxins 1-naphthaleneacetic acid (1-NAA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) had strong stimulatory effects on ethylene induction surpassing that of IAA. Both 2-naphthalaneacetic acid (2-NAA) and 2, 6-dichlorophenoxy acetic acid (2, 6-D), structural analogs of these auxins, respectively, were found to be inactive. Treatment with NPA, a strong inhibitor of polar auxin transport, led to drastic increase in IAA-induced ethylene production while it has bo effect on ethylene production induced by 1-NAA. A positive correlative existed between intracellular auxin level and ethylene production.

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Shoot Proliferation of Populus euramericana(Populus deltoides X P. nigra) through in vitro Tissue Culture

  • Kang, Ho-Duck;Moon, Heung-Kyu
    • Plant Resources
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    • v.4 no.3
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    • pp.111-120
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    • 2001
  • The efficiency of in vitro regeneration of four clones of Populus euramericana, Canada blanc, Eugenii, I-45/51, and Wisconsin #5, was examined. Cytokinins and the combinations with auxins affected the rate of regeneration from the explants of root segments, stem internodes, and leaf discs. Overall, BA and the combination with auxins were effective in root segments and leaf discs of the Canada blanc clone, whereas zeatin and the combination with auxins were important in stem internodes of the Wisconsin #5 clone. The highest number of shoots averaging 17.6 $\pm$ 0.47 from root segments in the Canada blanc clone,18.2 $\pm$ 3.0 from stem internodes in the Wisconsin #5 clone, and 17.8 $\pm$ 1.92 from leaf discs in the Canada blanc clone were obtained with 2.0 mg/1 BA, 2.0 mg/l zeatin combined with 0.2 mg/l IAA, and 0.5 mg/l BA combined with 0.05 mg/l 2,4-D, respectively. In particular, the addition of 2,4-D into cytokinin medium promoted shoot proliferation.

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Production of Anthraquinone Derivatives by Hairy Roots of Rubia cordifolia var. pratensis (갈퀴꼭두선이의 Hairy Root 배양에 의한 Anthraquinone계 색소생산 연구(II))

  • Kim, You-Sun;Shin, Seung-Won
    • Korean Journal of Pharmacognosy
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    • v.27 no.4
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    • pp.301-308
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    • 1996
  • Hairy roots induced from stems of Rubia cordifolia var. pratensis were cultured in the liquid medium under a variety of auxins to find the optimal condition for the growth and production of pigments. Culture of the hairy roots on NN liquid medium containing NAA 0.5 mg/l was best for growth of hairy roots. Production of yellow anthraquinone derivatives and purpurin in hairy roots was enhanced by the culture on NN liquid medium without auxins. Effects of L-phenylalanine, L-tyrosine and juglone, synthesized via the shikimic acid pathway, on growth and production of pigments in hairy roots were studied in the present study. Concentration of exogeneous L-phenylalanine. L-tyrosine and juglone in liquid culture system of hairy root containing NAA 0.1 mg/l was decreased quickly in its early stages of the culture period. Addition of juglone to NN liquid medium containing NAA 0.1 mg/l enhanced the productivity of pigments in hairy roots.

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Hormonal Effect and Cytokinin Autonomy in callus Culture of Phaseolus vulgaris L. (식물 Hormone의 영향과 Cytokinin Autonomy)

  • 김상구
    • Journal of Plant Biology
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    • v.25 no.4
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    • pp.161-168
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    • 1982
  • The activities of auxins and cytokinins have been examined in the growth of callus tissue derived from Phaseolus vulgaris L. cv. Damyang. The synthetic auxin, picloram was the most effective in promoting callus growth and the range of effective concentrations (0.1$\mu{M}$ to 32$\mu{M}$) was broad. 2, 4-D also enhanced callus growth at the optimal concentration of 3.2$\mu{M}$. NAA promoted callus growth at relatively higher concentrations than other auxins tested. IAA was less effective in supporting callus growth. Cytokinin bearing saturated side chain ($N^6$-isopentyladenine) was approximately 30 times more active than the corresponding unsaturated compound, $N^6$-($\D^2$-isopentenyl) adenine. The abilities of cytokinin-autonomous growth were also examined. Callus tissues previously grown on concentrations lower and/or higher than optimal concentrations of cytokinins were better habituated in the subsequent passage. It was suggested that the development of cytokinin autonomy may be related to dosage-concentrations of cytokinin in the previous passage.

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Effects of Auxins end Cytokinins on Callus Induction from Leaf Blade, Petiole, and Stem Segments of in Vitro-grown 'Sheridan' Grape Shoots

  • Seung-Heui kim;Kim, Seon-Kyu
    • Journal of Plant Biotechnology
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    • v.4 no.1
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    • pp.17-21
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    • 2002
  • To establish an the mass production system of grape anthocyanin pigments through callus and cell suspension culture, the effects of various combinations of auxins and cytokinins on friable callus production were studied. for friable callus production, 2,4-D was superior to other regulators. IAA at 2 mg/L induced callus from stem and petiole while NAA resulted in rooting. Callus induction rate increased with the 2,4-D level, and stem segments were superior to leaf blade or petiole, showing nearly 100% with 1 and 2 mg/L 2,4-D from petiole and stem. Combined treatments of 2,4-D + kinetin and NAA + BA also yielded friable callus from stem segments. In treatments with 1 mg/L 2,4-D + 1 mg/L kinetin and 1 mg/L NAA + 1 mg/L BA, callus induction rate was nearly 100%. The combination effect of 2,4-D and BA on anthocyanin production was not significant.

Studies on the Vegetative Propagation of Korean Ginseng (Panax ginseng C. A. Meyer) II. Effects of Synthetic Auxins on the Rooting and the Root Growth after Rooting from Ginseng Stem Cutting. (고려인삼의 영양번식에 관한 연구 제2보 합성 Auxin의 처리가 인삼경삽수의 발근 및 발근후 근의 생장에 미치는 영향)

  • Jaeseng, Jo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.4
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    • pp.504-508
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    • 1983
  • To define the effects of synthetic auxins on rooting from the ginseng stern cutting and the root growth in diameter after the rooting of the cuttings, stern cuttings with palmate leaves obtained from seedlings and 2-year old ginseng plants were planted in rooting media treated with solutions of the synthetic auxins. All the roots induced from the cuttings were adventitious fibrous roots at first, but a few adventitious roots of the cutting were thickened in diameter to over 2 to 3mm at 120 days after cutting and the rest of them disappeared. IBA was the most effective auxin for rooting and root growth in diameter after rooting from the cuttings. The shape of the roots that thickened in diameter could be divided into two types. Both types of thickened roots were fully lignified at 120 days after cutting and those thickened roots did not dry up or die by July of the next year, however no rhyzomes or shoot primodia were induced from them.

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Interaction between Brassinolide and Auxins on Bioassays (Brassinolide와 Auxin과의 상호작용의 생물검정에 의한 평가)

  • Choi, Chung-Don;Kim, Soon-Chul;Lee, Soo-Kwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.58-64
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    • 1990
  • This study was conducted to investigate the interaction effect of a new plant growth regulator brassinolide and auxins using several bioassay methods such as rice lamina inclination test, oat coleoptile segment growth test and radish hypocotyl elongation test. For rice lamina inclination test, the antagonistic response showed at low concentration combinations of homobrassinolide and auxins (IAA and 2,4-D), while the combinations of high concentration responsed mostly synergistic or additive effects, IAA was generally higher than 2,4-D for combination effect with homobrassinolide. For oat coleoptile segment growth test, the synergistic effect showed at IAA concentrations less than 0.1ppm while additive response exhibited above 0.3ppm regardless of homobrassinolide concentrations. In radish hypocotyl elongation test, the interaction response varied with cotyledon. The sections removed cotyledon showed mostly antagonistic effects, except for combination of homobrassinolide with IAA 3ppm. Interaction responses were Quite different from bioassay methods, particularly using experimental materials: antagonistic responses were usually shown at the section that attached growing point while these for sections that removed growing point were responded synergistic or additive effect due to unstable endogenous hormones.

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Bioassays of Plant Hormones and Plant Growth Regulating Substances I . Auxins, Gibberellins, and Cytokinins (식물홀몬 및 생장조절물질의 생물검정기술 I. 옥신, 지베렐린 및 싸이토키닌)

  • 이정명
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s01
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    • pp.4-15
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    • 1989
  • The objective of this paper is to compare and summarize the procedure and effectiveness of some bioassay systems and to point out ways to obtain reliable results from each bioassay. Detailed C:escriptions were given for those widely-adapted bioassay methods, such as mungbean rooting (auxin), Avena first internode straight growth (auxin), dwarf rice growth (gibberellin), dwarf pea epicotyl elongation (gibberellin), radish cotyledon expansion test (cytokinin), and tobacco stem pith callus growth (cytokinin), and the effects of various plant growth regulators including some recently introduced growth retardants (Paclobutrazol, Uniconazol, etc.) were also summarized.

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