• Bulletin of the Korean Chemical Society
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• 제25권9호
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• pp.1303-1304
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• 2004

• Bulletin of the Korean Chemical Society
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• 제29권2호
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• pp.342-346
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• 2008

Aurintricarboxylic acid (ATA) prevents apoptosis in a wide range of cell types, including PC12 cells. ATA is known to increase the phosphorylation level of IGF-1 receptor (IGF-1R) and downstream signaling proteins. ATA can translocate across the plasma membrane of PC12 cells and inhibit protein tyrosine phosphatases (PTPs) and, therefore, it is not clear whether ATA exerted its antiapoptotic effect through activation of IGF-1R or by inhibition of cytosolic PTPs. When PC12 cells, deprived of serum, were treated with Fab fragment of anti-IGF-1R antibody to prevent the binding of ATA to the extracellular domain of IGF-1R, ATA was found to penetrate into the cytosolic space of the cells. Under these conditions, the survival-promoting effects of ATA were abolished, and the increase of phosphorylation and characteristic cleavage of IGF-1R were not observed. These results indicate that the antiapoptotic effect of ATA in PC12 cells is due to the binding of ATA to the extracellular domain of IGF-1R and subsequent activation of the IGF-1R, not inhibition of cytosolic PTP(s).

• 미생물학회지
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• 제50권4호
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• pp.372-375
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• 2014

본 연구에서는 백색부후균인 겨울우산버섯의 원형질체 분리 조건과 유전자 형질전환 방법을 확립하였다. 겨울우산버섯 균사에 세포벽 분해효소로 0.5% Usukizyme을 처리하여 ml당 $1{\times}10^7/ml$개의 원형질체를 확보할 수 있었다. 형질전환체의 선별을 위해 hygromycin에 대한 저항성을 갖는 유전자(hph)를 선택표지로 이용하여 형질전환용 벡터(pHYgpt)를 제작하였다. 40% polyethylene glycol 용액과 aurintricarboxylic acid와 heparin, supermidine을 첨가하여 형질전환을 수행 한 결과 벡터 $1{\mu}g$ 당 100-160개의 수율로 형질전환체를 얻었다. 도입된 벡터는 hph 유전자의 PCR 증폭을 통해 형질전환체의 염색체내에 삽입되었음을 확인하였다. 이러한 결과는 polyporus 속의 유전자를 활용한 새로운 균주 개발에 유용한 기술이 될 것이다.