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Transesterification Using the Cross-Linked Enzyme Aggregate of Photobacterium lipolyticum Lipase M37

  • Han, Jin-Yee;Kim, Hyung-Kwoun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.11
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    • pp.1159-1165
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    • 2011
  • Biodiesel is methyl and ethyl esters of long-chain fatty acids produced from vegetable oils or animal fats. Lipase enzymes have occasionally been used for the production of this biofuel. Recently, biodiesel production using immobilized lipase has received increased attention. Through enhanced stability and reusability, immobilized lipase can contribute to the reduction of the costs inherent to biodiesel production. In this study, methanol-tolerant lipase M37 from Photobacterium lipolyticum was immobilized using the cross-linked enzyme aggregate (CLEA) method. Lipase M37 has a high lysine content (9.7%) in its protein sequence. Most lysine residues are located evenly over the surface of the protein, except for the lid structure region, which makes the CLEA preparation yield quite high (~93%). CLEA M37 evidences an optimal temperature of $30^{\circ}C$, and an optimal pH of 9-10. It was stable up to $50^{\circ}C$ and in a pH range of 4.0-11.0. Both soluble M37 and CLEA M37 were stable in the presence of high concentrations of methanol, ethanol, 1-propanol, and n-butanol. That is, their activities were maintained at solvent concentrations above 10% (v/v). CLEA M37 could produce biodiesel from olive oil and alcohols such as methanol and ethanol. Additionally, CLEA M37 generated biodiesel via both 2-step methanol feeding procedures. Considering its physical stability and reusability, CLEA M37 may potentially be used as a catalyst in organic synthesis, including the biodiesel production reaction.

Nonsurgical endodontic retreatment of fused teeth with transposition: a case report

  • Cardoso, Miguel Agostinho Beco Pinto;Noites, Rita Brandao;Martins, Miguel Andre Duarte;Paulo, Manuel Pedro da Fonseca
    • Restorative Dentistry and Endodontics
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    • v.41 no.2
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    • pp.148-153
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    • 2016
  • Tooth transposition is a disorder in which a permanent tooth develops and erupts in the normal position of another permanent tooth. Fusion and gemination are developmental disturbances presenting as the union of teeth. This article reports the nonsurgical retreatment of a very rare case of fused teeth with transposition. A patient was referred for endodontic treatment of her maxillary left first molar in the position of the first premolar, which was adjacent to it on the distobuccal side. Orthopantomography and periapical radiography showed two crowns sharing the same root, with a root canal treatment and an associated periapical lesion. Tooth fusion with transposition of a maxillary molar and a premolar was diagnosed. Nonsurgical endodontic retreatment was performed. At four yr follow-up, the tooth was asymptomatic and the radiolucency around the apical region had decreased, showing the success of our intervention. The diagnosis and treatment of fused teeth require special attention. The canal system should be carefully explored to obtain a full understanding of the anatomy, allowing it to be fully cleaned and obturated. Thermoplastic techniques were useful in obtaining hermetic obturation. A correct anatomical evaluation improves the set of treatment options under consideration, leading to a higher likelihood of esthetically and functionally successful treatment.

Structural and temperature coefficient of resistance characteristics of colossal magnetoresistance Mn oxides prepared by RF sputtering

  • Choi, Sun-Gyu;Ha, Tae-Jung;Reddy, A.Sivasankar;Yu, Byoung-Gon;Park, Hyung-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.11a
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    • pp.361-361
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    • 2007
  • A lot of efforts have been paid to develop infrared imaging systems in last decades. Bolometer has a wide range of applications from military to commercial, such as military night vision, medical imaging system and so on. Bolometer is a resistive sensor that detects temperature changes through resistance change. To improve detecting ability, bolometer should have a good resistive film which has high temperature coefficient of resistance (TCR) value. Colossal magnetoresistance (CMR) $L_{1-x}A_xMnO_3$ (where L and A are trivalent rare-earth ions and divalent alkaline earth ions, respectively.) are received attention to apply bolometer resistive film because it has a high TCR property which was discovered in the metal to semiconductor phase transition temperature region. In this work, CMR films were deposited on various substrates in relative low substrate temperature by RF magnetron sputtering. The influence of deposition parameters such as substrate temperature, gas partial pressure, and so on have been studied. The structural and TCR properties of the films were also investigated for applying to microbolometer.

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A Study On the Application Methods of a Support Vector Machine for Gene Promoter Prediction. (유전자 프로모터 예측을 위한 Support Vector Machine의 응용 방법에 대한 연구)

  • Kim, Ki-Bong
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.714-718
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    • 2007
  • The high-throughput sequencing of a lot of genomes has resulted in the relatively rapid accumulation of an enormous amount of genomic sequence data. In this context, the problem posed by the detection of promoters in genomic DNA sequences via computational methods has attracted considerable attention in recent years since exact promoter prediction can give a clue to the elucidation of overall genetic networks. In this study, applications of support vector machine(SVM) to promoter prediction are explored to show a right approaches to discriminate between promoter and non-promoter regions by means of SVM. The results of various experiments show that encoding method, encoding region and learning data constitution can play an important role in the performance of SVM.

Study on the colors of Kim Whan-ki's painting (색으로 본 김환기의 작품 세계)

  • Kim, Hyun-Suk
    • The Journal of Art Theory & Practice
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    • no.3
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    • pp.155-172
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    • 2005
  • Kim Whan-ki is an unusual instance in Korean modern artists, who payed attention to emotional and expressive effects of colors. The color of Whan-ki's paintings have been recognized as linked with 'blue' in spite that he used colors within the category of 'Colors of Five Directions(五方色)', which are traditional oriental colors composed of red, lue, yellow, white, green and black. Kim Whan-ki unearthed upon similarity of Five Directions Colors to the three(five) primary colors which modern abstract painter like Mondrian layed down. Whan-ki switched the five directions colors to modern ones. Kim Whan-ki's dot painting in which pure and watery color is sucked in ground is modernistic adaptation from ink painting. He packs a dot with sky and earth, moon and stars, forest and tree, birds and flowers, friends at his hometown, wind, sound and so on. Putting tens of thousands of these shapes and colors into a dot is modernistic version from ink painting. In that point there is a possibility to say that 'dark blue' of the dot painting is 'Hyun-saec(玄色)'. Eventually we can make sure that Kim Whan-ki's view of Art originated in oriental philosophy and beauty.

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Enhanced Electromagnetic Properties of Nickel Nanoparticles Dispersed Carbon Fiber via Electron Beam Irradiation (전자선 안정화에 의한 니켈 나노 입자가 분산된 탄소섬유의 전자기적 특성 향상)

  • Lee, Yeong Ju;Kim, Hyun Bin;Lee, Seung Jun;Kang, Phil Hyun
    • Journal of Radiation Industry
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    • v.9 no.1
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    • pp.15-20
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    • 2015
  • Carbon fiber has received much attention owing to its properties, including a large surface-to-volume ratio, chemical and thermal stability, high thermal and electrical conductivity, and high mechanical strengths. In particular, magnetic nanopowder dispersed carbon fiber has been attractive in technological applications such as the electrochemical capacitor and electromagnetic wave shielding. In this study, the nickel-oxide-nanoparticle dispersed polyacrylonitrile (PAN) fibers were prepared through an electrospinning method. Electron beam irradiation was carried out with a 2.5 MeV beam energy to stabilize the materials. The samples were then heat-treated for stabilization and carbonization. The nanofiber surface was analyzed using a field emission scanning electron microscope (FE-SEM). The crystal structures of the carbon matrix and nickel nanopowders were analysed using X-ray diffraction (XRD). In addition, the magnetic and electrical properties were analyzed using a vibrating sample magnetometer (VSM) and 4 point probe. As the irradiation dose increases, the density of the carbon fiber was increased. In addition, the electrical properties of the carbon fiber improved through electron beam irradiation. This is because the amorphous region of the carbon fiber decreases. This electron beam effect of PAN fibers containing nickel nanoparticles confirmed their potential as a high performance carbon material for various applications.

Numerical investigation of film boiling heat transfer on the horizontal surface in an oscillating system with low frequencies

  • An, Young Seock;Kim, Byoung Jae
    • Nuclear Engineering and Technology
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    • v.52 no.5
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    • pp.918-924
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    • 2020
  • Film boiling is of great importance in nuclear safety as it directly influences the integrity of nuclear fuel in case of accidents involving loss of coolant. Recently, nuclear power plant safety under earthquake conditions has received much attention. However, to the best of our knowledge, there are no existing studies reporting film boiling in an oscillating system. Most previous studies for film boiling were performed on stationary systems. In this study, numerical simulations were performed for saturated film boiling of water on a horizontal surface under low frequencies to investigate the effect of system oscillation on film boiling heat transfer. A coupled level-set and volume-of-fluid method was used to track the interface between the vapor and liquid phases. With a fixed oscillation amplitude, overall, heat transfer decreases with oscillation frequency. However, there is a frequency region in which heat transfer remains nearly constant. This lock-on phenomenon occurs when the oscillation frequency is near the natural bubble release frequency. With a fixed oscillation frequency, heat transfer decreases with oscillation amplitude. With a fixed maximum amplitude of the additional gravity, heat transfer is affected little by the combination of oscillation amplitude and frequency.

Exploration into Pre-service Early Childhood Teachers' Experience of Participation in Health Promotion Activities and Its Meaning (건강증진활동에 참여한 예비유아교사의 경험과 의미 탐색)

  • Ahn, Hye Jung
    • Korean Journal of Childcare and Education
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    • v.17 no.5
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    • pp.105-128
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    • 2021
  • Objective: This study aimed to explore the experience and meaning of pre-service early childhood teachers who practiced health promoting activities for themselves. Methods: Research participants were 115 pre-service early childhood teachers enrolled in the health education for children course at one college in G region. The reports of activity records of their eight-week long health promotion activities were collected as qualitative data followed by content analysis being implemented. Results: First, the experience of the health promoting activities the participants took part in was categorized into 'Early phase of experience: A mix of worries and anticipation, and success and failure', 'Mid phase of experience: Being motivated by physical changes and records', and 'Late phase of experience: Continuous execution through habituation.' Second, the meaning of health promotion activities the participants took part in was categorized into 'A great opportunity for introspection', 'A meaningful start of college life', and 'A valuable chance to consider desirable roles of a teacher for early childhood health education.' Conclusion/Implications: The results of this study suggest that pre-service early childhood teachers should pay close attention to their health management, and that contents that emphasize the importance of teachers' health should be included in health education.

Management Software Development of Hyper Spectral Image Data for Deep Learning Training (딥러닝 학습을 위한 초분광 영상 데이터 관리 소프트웨어 개발)

  • Lee, Da-Been;Kim, Hong-Rak;Park, Jin-Ho;Hwang, Seon-Jeong;Shin, Jeong-Seop
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.6
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    • pp.111-116
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    • 2021
  • The hyper-spectral image is data obtained by dividing the electromagnetic wave band in the infrared region into hundreds of wavelengths. It is used to find or classify objects in various fields. Recently, deep learning classification method has been attracting attention. In order to use hyper-spectral image data as deep learning training data, a processing technique is required compared to conventional visible light image data. To solve this problem, we developed a software that selects specific wavelength images from the hyper-spectral data cube and performs the ground truth task. We also developed software to manage data including environmental information. This paper describes the configuration and function of the software.

Unilateral Biportal Endoscopic Spinal Surgery Using a 30° Arthroscope for L5-S1 Foraminal Decompression

  • Kim, Ju-Eun;Choi, Dae-Jung
    • Clinics in Orthopedic Surgery
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    • v.10 no.4
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    • pp.508-512
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    • 2018
  • Foraminal decompression using a minimally invasive technique to preserve facet joint stability and function without fusion reportedly improves the radicular symptoms in approximately 80% of patients and is considered one of the good surgical treatment choices for lumbar foraminal or extraforaminal stenosis. However, proper decompression was not possible because of the inability to access the foramen at the L5-S1 level due to prominence of the iliac crest. To overcome this challenge, endoscopy-based minimally invasive spine surgery has recently gained attention. Here, we report the technical skills required in unilateral extraforaminal biportal endoscopic spinal surgery using a $30^{\circ}$ arthroscope to enable foraminal decompression at the L5-S1 level. Two 0.8-cm portals were created 2 cm lateral from the lateral border of the pedicles at the L5-S1 level. After sufficient working space was made, half of the superior articular process (SAP) in the hypertrophied facet joint was removed using a high-speed burr and a 5-mm wide osteotome, whereas the remaining inside part of the SAP was removed using a Kerrison punch and pituitary punch. The foraminal ligamentum flavum should be removed to inspect the conditions of the L5 exiting root and disc. Removing of the extruded disc could decompress the L5 root. The extraforaminal approach using a $30^{\circ}$ arthroscope is considered a minimally invasive alternative technique for decompressing foraminal stenosis at the L5-S1 level that preserves facet stability and provides symptomatic relief.