• Macromolecular Research
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• 제15권4호
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• pp.348-356
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• 2007

The aim of this study was to fabricate a submicro-and micro-patterned fibronectin coated wafer for a cell culture, which allows the positions and dimensions of the attached cells to be controlled. A replica molding was made into silicon via a photomask in quartz, using E-beam lithography, and then fabricated a polydimethylsiloxane stamp using the designed silicon mold. Hexadecanethiol $[HS(CH_2){_{15}}CH_3]$, adsorbed on the raised plateau of the surface of polydimethylsiloxane stamp, was contact-printed to form self-assembled monolayers (SAMs) of hexadecanethiolate on the surface of an Au-coated glass wafer. In order to form another SAM for control of the surface wafer properties, a hydrophilic hexa (ethylene glycol) terminated alkanethiol $[HS(CH_2){_{11}}(OCH_2CH_2){_6}OH]$ was also synthesized. The structural changes were confirmed using UV and $^1H-NMR$ spectroscopies. A SAM terminated in the hexa(ethylene glycol) groups was subsequently formed on the bare gold remaining on the surface of the Aucoated glass wafer. In order to aid the attachment of cells, fibronectin was adsorbed onto the resulting wafer, with the pattern formed on the gold-coated wafer confirmed using immunofluorescence staining against fibronectin. Fibronectin was adsorbed only onto the SAMs terminated in the methyl groups of the substrate. The hexa (ethylene glycol)-terminated regions resisted the adsorption of protein. Human dermal fibroblasts (P=4), obtained from newborn foreskin, only attached to the fibronectin-coated, methyl-terminated hydrophobic regions of the patterned SAMs. N-HDFs were more actively adhered, and spread in a pattern spacing below $14{\mu}m$, rather than above $17{\mu}m$, could easily migrate on the substrate containing spacing of $10{\mu}m$ or less between the strip lines.

• Human Ecology Research
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• 제53권2호
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• pp.169-177
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• 2015

The PRQ instrument is parental perspectives of the parent-child relationship. It measured parent-child dimensions that include attachment and involvement. Also, it provides additional information on parenting style, parenting stress and satisfaction with the child's school. This study tested reliability and validity of the Korean version of Parenting Relationship Questionnaire-Child (K-PRQ-C). The K-PRQ-C was administered to parents of 713 elementary school children including 362 boys and 351 girls. They were from 1st to 6th grade children who were recruited form 4 elementary schools in Korea. Reliability was tested through internal consistency. We conducted confirmatory factor analysis. Moreover, scale inter-correlation was conducted for validity test. Discriminant validity conducted through administration to the parents of clinical children with emotional and behavior disorders. Construct validity was also examined through Korean version of the Parenting Sense of Competence. The results of confirmatory factor analysis indicated that the similar factor structures exist for PRQ-C and K-PRQ-C. The results showed a high level of internal consistency as well as construct and discriminant validity. These results showed valid psychometric properties for the K-PRQ-C. The K-PRQ-C provides a reliable and valid means of gathering information about the parent-child relationship. We discussed Implications for the use of the K-PRQ-C as a clinical tool and as a research instrument.

• 한국융합학회논문지
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• 제10권12호
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• pp.177-181
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• 2019

본 연구는 광중합기의 청색광을 이용한 치과 치료과정에서 광중합기용 팁들과 보안경의 청색광 차단정도를 알아보기 위해, UV-Vis 스펙트로미터 기기에 광중합기용 팁들과 보안경들을 위치시키고 빛을 투과하여 측정한 결과를 비교 분석하였다. 분석결과, 분석에 사용한 4가지 종류의 광중합기용 팁들 모두 청색광 차단효과가 매우 우수한 것으로 나타났다. 보안경의 경우 붉은색 계열 보안경이 노란색 계열 보안경보다 광중합기용 팁들과 유사한 청색광 투과율을 보여 청색광 차단효과가 더 우수한 것으로 나타났다. 결과적으로 청색광 차단정도가 높은 광중합기용 팁 부착과 보안경 착용이 눈 보호를 위해 필요한 행위임을 추천한다. 이러한 행위는 눈에 대한 지속적인 자극과 피로감을 줄여줄 것이라 사료된다.

• 한국구조물진단유지관리공학회 논문집
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• 제24권2호
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• pp.1-8
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• 2020

원자력발전소와 같은 발전플랜트 시설의 경우 건물 자체뿐만 아니라 내부에 설치된 비구조요소의 내진안정성 또한 중요하다. 특히, 전기기기가 부착되는 캐비닛 구조물 등은 지진에 대비하여 기능적 안전성이 확보되어야 한다. 이에 따라 본 연구에서는 기존 발전플랜트 내부에서 운용 중인 전기기기 캐비닛 구조물의 동특성을 파악하고, 동적거동 시 발생되는 응답 수준을 분석하였다. 그리고 내진보강 방법 중 제진 방식을 적용하기 위해 강재 플레이트 댐퍼를 채택하고 대상기기의 크기에 적합하게 조정하였다. 또한, 최적의 보강방안을 도출하기 위해 부착위치에 따른 변수를 설정하고 보강 전·후의 지진동 입력에 의한 응답에 대하여 분석하는 연구를 수행하였다.

• 분석과학
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• 제29권5호
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• pp.225-233
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• 2016

입자 표면에 고분자를 화학적으로 접목하는 것은 수용성 액체 이상 시스템(ATPS)과 같이 분리된 액체 층에서 마이크로/나노입자를 선택적으로 분배시키는데 중요한 구실을 한다. 본 연구에서는 덱스트란, 폴리에틸렌 글리콜, 알부민을 화학적으로 폴리스티렌 자성 마이크로입자 표면에 화학적으로 부착시켰다. 분배 현상을 역전시킬 수 있는 긴 고분자 사슬의 부착은 일차 아민 작용기를 가진 다양한 고분자를 p-톨루엔술폰산(토실)기를 부착시킨 폴리스티렌 자성 마이크로입자와의 S_N2 치환에 의해 간단히 수행될 수 있었다. 이 후 적외선 현미경을 사용하여 마이크로입자의 표면 변화의 유무를 검사하였다. 반응 후 입자들은 세 가지 폴리머 모두 N-H 신축 진동 피크를 보였으며 대부분의 주요 피크들의 위치는 반응 전후에 유사하였으나 지문 영역에서 구별 가능한 차이를 보였다.

• Macromolecular Research
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• 제13권5호
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• pp.446-452
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• 2005

The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/$cm^2$. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control

• Child Health Nursing Research
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• 제8권3호
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• pp.322-333
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• 2002

Purpose: The purpose of study was : 1) to analyse the trend of research on the sensory stimulation in newborn in Korea, 2) to suggest direction for further study on the sensory stimulation in newborn and contributing to the use of intervention in newborn. Method: Research studies on the sensory stimulation in newborn were selected from the dissertations, parents-child health. Academic Society Journal, and from Korean Journal of Pediatrcs which were conducted between 1984 and 2002. The total numbers of the studies were 15. These studies were analyzed for 1) time of publication or presentation 2) thesis for a degree or nondegree 3) characteristics of subjects 4) sample size 5) research design 6) type of sensory stimualtion 7) meaurement tool 8) result. Result : 1)The most year of studies were the 1996's～ 2000's. 2) The degree of studies were higher than nondegree. 3) The most characteristics of subjects were the premature infants. 4) The most frequent sample sizes were 11-20. 5) The all research design was nonequivalent control group pretest-posttest design in quasi experimental basis. 6) The most type of sensory stimulation was Field massage(tactile and kinesthetic). 7) The measurement tool used all objective tools. 8) The most research data were collected by the measure of the weight, heart rate, behavior state, respiration, stress hormon and positive results. Conclusion: 1) Human sensory stimulation did not seem to harm the infants. 2) several positive effects of sensory stimulation that have been reported previously must be reevaluated. 3) further research will be required large sample size, long-term outcome, individualized stimulation program, early parent-infant contact in order to enhance the development of attachment, to determine the underlying mechanism and theorical frame for these stimulation effect.

• International Journal of Knowledge Content Development & Technology
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• 제3권2호
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• pp.67-94
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• 2013

This exploratory research explores the concept of a digital legacy as a general concept and as a collection of digital possessions with unique characteristics. The results reported in this article are part of a larger study. In Cushing (2013), the author identified the characteristics of a digital possession. In this study, these characteristics of a digital possession were utilized to explore how the characteristics of several digital possessions could form a collection, or a digital legacy. In addition to being explored as a collection of digital possessions, data was collected about the general concept of a digital legacy. In part I of the study, 23 participants from three age groups were interviewed about their general concept of a digital legacy. Five general characteristics describing a digital legacy were identified. In part II of the study, interview data from Cushing (2013) was used to create statements describing digital possessions. The statements were classified utilizing the archival concept of primary and secondary values, as well as the consumer behavior concepts of self extension to possessions and possession attachment. Primary value refers to the purpose for which the item was created, while secondary value refers to an additional value that the participants can perceive the item to hold, such as a perception that an item can represent one's identity. Using standard Q method procedure, 48 participants were directed to rank their agreement with 60 statements (written on cards), along a distribution of -5 to +5, according to the characteristics of the digital possession they would most like to maintain for a digital legacy. The ranked statements were analyzed using Q factor analysis, in order to perceive the most common statements associated with maintaining digital possessions for a digital legacy. Q method results suggested that most individuals described the digital possessions they wanted to maintain for a digital legacy using various combinations of characteristics associated with primary and secondary values. This suggests that while some participants will respond to personal archiving based on the concept of preserving identity (a perceived secondary value), this will not appeal to everyone. Information professional could consider this difference in appeal when marketing personal archiving assistance to patrons.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제21권4호
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• pp.338-342
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• 2015

시계열 데이터를 다룰 수 있는 기계학습모델인 회귀 신경망은 되먹임 연결을 허용하기 때문에 앞먹임 신경망에 비해 훨씬 다양한 구조를 가질 수 있다. 본 연구에서는 은닉 뉴런 간의 네트워크 구조에 초점을 맞추어 그것이 회귀 신경망의 정보처리 능력에 미치는 영향을 탐구하고자 한다. 이를 위해 회귀신경망 모델 중 하나인 Echo State Network을 기준으로 하여, 여러 가지 잘 알려진 네트워크 모델에 따라 은닉 뉴런 간 연결을 구성하고 각각의 경우에 시계열 학습 능력과 동역학을 분석하였다. 그 결과, 은닉 뉴런의 네트워크 구조에 따라 모델의 성능이 큰 폭으로 변하는 것이 관찰되었으며, 그러한 현상은 신경망 동역학이 가지는 임계도(criticality)의 변화와 잘 일치했다. 본 연구의 결과는 기존 회귀 신경망 연구에서 주된 관심사였던 신경망 연결 가중치뿐만 아니라 신경망의 연결 구조가 모델의 성능에 중요한 영향을 미친다는 사실을 보여주며, 성능 향상을 위한 중요한 단서가 될 수 있다.

• 한국아동복지학
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• 제55호
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• pp.143-171
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• 2016

본 연구는 청소년의 학교폭력 가해행동에 대한 청소년 개인요인, 학교요인, 지역요인의 영향을 검증하기 위해 2수준 다층모형을 적용하였고, HLM 7 프로그램으로 분석하였다. 이를 위해 서울 및 경기지역 50개 중학교의 총 1,777부 학생자료가 분석에 사용되었다. 2수준 다층모형 분석결과, 학교폭력 가해행동에 영향을 미치는 청소년 개인요인으로는 폭력에 대한 태도, 부모 애착, 아동학대 경험, 비행친구접촉으로 나타났고, 학교요인으로는 교사의 폭력적 훈육으로 나타났다. 또한 본 연구에서 지역사회 요인으로 주목한 지역무질서가 청소년의 학교폭력 가해행동에 정적으로 유의한 영향을 미치는 것으로 확인되었다. 본 연구 결과는 학교폭력 원인의 이해와 그에 따른 대책마련을 위해서는 청소년 개인, 가정, 학교 외에도 지역사회 등 청소년의 일상생활이 이루어지는 다양한 환경적 맥락을 종합적으로 고려해야 함을 제시한다. 본 연구결과를 바탕으로 학교폭력을 효과적으로 해소하기 위한 정책 및 실천 방안이 논의되었다.