• ALGAE
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• 제25권1호
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• pp.37-44
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• 2010

A tide-simulating apparatus was developed for culturing marine macroalgae. The objective of this study was to introduce a novel tide-simulating apparatus that can simulate a diurnal or semi-diurnal tidal cycle in the laboratory. In this apparatus, the seaweeds are move up and down and the water level remains the same during the simulated tidal cycle. The apparatus consists of 18 cylindrical culture tanks (3 blocks $\times$ 6 culture tanks) with 12 cm diameter and 24.5 cm long containing up to 2.5 L of seawater. There is a horizontal plate which covered all 18 culture tanks, and it is raised and lowered by a programmable motor that can regulate exposure time. In one application, seaweeds are attached to braided twine hung on Plexiglas air-tubing. The air-tubing is attached to a lid that is set on a horizontal plate. This apparatus is made of colorless Plexiglas to maximize light transmittance. This apparatus is easily disassembled and transportable to any indoor laboratory, wet laboratory, greenhouse, etc. This apparatus also offers considerable flexibility in terms of design. The size of culture tank can be redesigned by either increasing the height of cylinder or/and using a different diameter of cylindrical Plexiglas, therefore, larger/taller thalli can be cultivated. Growth rates of three eulittoral Porphyra species from different tidal elevations have been compared using this device.

• ALGAE
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• 제36권2호
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• pp.147-154
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• 2021

Kelp life history pathways alternate between macroscopic sporophytes that produce spores and microscopic gametophytes that produce gametes. Occasionally, an alternative pathway is seen. This study examined the circumstances by which the high latitude estuarine bull kelp, Nereocystis luetkeana, foregoes the "free-living microscopic stages by releasing embryonic sporophytes directly from sori. Sori were collected from adult N. luetkeana sporophytes from eight locations within Kachemak Bay, Alaska in 2018 and 2020 to examine spatial and temporal development of embryonic sporophytes on sori. Distinctions were made between sori collected from first-generation and overwintered adults to assess the influence of parental age on embryonic sporophyte release. Further distinctions were made between sori collected from attached and drifting individuals to assess the influence of the status of parental attachment to substrate on embryonic sporophyte release. Inspection of propagules released from sori after 48-h incubations indicated that embryonic sporophytes were occasionally released alongside viable spores. Though embryonic sporophytes were released from sori as early as spring, it was not evident that they were bound by seasonal or spatial limits. The percent of propagules that were embryonic sporophytes ranged from 0% to 100% but were not significantly different between first-generation and overwintered adults, nor were they different between attached and drifting individuals. Nevertheless, the characteristic of directly releasing embryonic sporophytes from adult sporophytes might have ecological advantages for N. luetkeana.

• 수산해양교육연구
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• 제25권6호
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• pp.1336-1347
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• 2013

The marine algal flora assemblages settled on the artificial seaweed reefs in Jeju-do before and after rocky cleaning were investigated during summer and the early succession of seaweeds attached to the test panels on the structures of the artificial reef after rocky cleaning were observed by visual observations and under the dissecting microscope in order to evaluate the effects of this rocky cleaning operation. Species composition and algal biomass on the Bboolsamgak-typed seaweed artificial reefs before and after rocky cleaning were measured and compared before and after rocky cleaning. Before rocky cleaning in July 2012, only nine algal species had existed on the Bboolsamgak-typed seaweed artificial reefs, however, twenty algal species occurred on the seaweed artificial reefs after rocky cleaning in July 2013. Particularly, 15 species among them in July 2013 were not found in July 2012 and they seemed to occur newly on the artificial reefs. A large quantity of algal biomass increased in July 2013 after rocky cleaning. Based on the importance value, there was a drastic change in the dominant species between July 2012 and July 2013 after rocky cleaning. By dissecting micrographs, an epiphytic diatom, Licmophora sp. and flagellates, were observed on the 9th day. A chlorophyte, Ulothrix flacca was observed after 14 days, Ulva pertusa, Bryopsis pulmosa, crustose coralline algae, and Cladophora spp. were after one month. After two months, a significant species succession was observed from three previous chlorophyte species to crustose coralline algae existed as a dominant species on the panels. In this study, however, any brown algae were not found until three months in the early seaweed succession. All above results indicates that the rocky cleaning is a very efficient method for the establishment of macroalgal assemblages on the artificial reefs.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1566-1573
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• 2014

Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger in attached systems owing to the more transparent water. Such higher availability of sunlight makes it possible to operate a raceway pond deeper than usual, resulting in a higher areal productivity. The attached system achieved 2.8-times higher biomass productivity and total lipid productivity of $9.1g\;m^{-2}day^{-1}$ and $1.9g\;m^{-2}day^{-1}$, respectively, than the suspended system. Biomass productivity can be further increased by optimization of the culture conditions. Moreover, algal biomass harvesting and dewatering were made simpler and cheaper in attached systems, because mesh-type substrates with attached microalgae were easily removed from the culture and the remaining treated wastewater could be discharged directly. When the algal biomass was dewatered using natural sunlight, the palmitic acid (C16:0) content increased by 16% compared with the freeze-drying method. There was no great difference in other fatty acid composition. Therefore, the attached system for algal cultivation is a promising cultivation system for mass biodiesel production.

• 한국해양학회지
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• 제21권1호
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• pp.25-33
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• 1986

미국 Texas 남쪽 해안에 위치한 Redfish Bay의 해초생태계에 우점종으로 나 타나는 4어종인 darter goby(Gobionellus bolesoma), pinfish(Lagodon rhomboides), code goby(Gobiosoma robustum), 그리고 Gulf pipefish(Syngnathus scovelli)에 대 한 성장에 따른 먹이 선택의 변화를 정량적으로 연구하였다. 가장 우점종인 darter goby는 비교적 다양한 먹이를 먹는 잡식성 어류로 amphipods, copepods, polychaetes, filamentous algae, diatoms 그리고 detritus등이 주된 먹이였다. 이 종 은 성장에 따른 먹이 선택의 변화가 크지 않았다. 반면에 pinfish, code goby 및 Gulf pipeifish는 성장함에 따라 먹이 종류가 크게 달라졌다. pinfish의 경우, 4단계 의 먹이 선택의 변화를 보이는데, 초기에는 육식성으로 주로 copepods를 섭식하였 으나, 조금 성장하면 주된 먹이가 amphipods로 바뀌며, 조금 더 성장하면 잡식성으 로 변해 filamentous algae, diatoms, amphipods 그리고 aolychaetes등의 다양한 먹 이를 먹으며, 그후 더욱 성장하면 seagrass와 seagrass위에 붙어있는 algae 그리고 그들의 debris를 주로 먹는 초식성으로 먹이 습성이 바뀌었다. code goby 와 Gulf popefish 는 비교적 적은 종류의 동물성 먹이에만 의존하는 육식성 어류들이었다.또한 두조은 비슷한 성장에 딸느 먹이 선택의 변화를 보이는데,초기에는 주로 copepods 를 섭이하였으나 성장함에 따라 점진적으로 보다 큰 amphipods로 먹이 선택이 바뀌었다.

• 환경생물
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• 제39권3호
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• pp.383-389
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• 2021

The Erythropeltidales are a common group of small, mostly epiphytic, marine red algae. However, they are little known in Korea. Many of the described species of Erythropeltidales differ subtly in morphology, and often the morphological differences are due to the substrate or environmental changes. Integration of molecular data with standardized culture conditions has been recommended to account for these algae. A Madagascaria species was first collected from the western coast of Korea and was identified as Madagascaria erythrocladioides based on the morphological and molecular characteristics. Morphological characteristics conformed well with its original description, and the phylogenetic analysis based on rbcL sequence showed Korean M. erythrocladioides nests in the same clade with the original species described in Japan with a genetic distance of 0.0-0.1%. This species was isolated from a red alga, Pterocladiella capillacea, in laboratory culture. The thallus ontogeny and host preference were examined by a co-culture with 13 different species of algae. Results showed a relatively broad host preference in mono-spore attachment and epiphyte development of Madagascaria erythrocladioides. Mono-spores of M. erythrocladioides attached to most of the red algal hosts' surfaces but no crustose thalli developed on some of the algal hosts even after one month of co-culture.

• ALGAE
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• 제20권1호
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• pp.15-23
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• 2005

Polysiphonia pacifica is rhodomelaceous red algal species that includes five varieties in Pacific Ocean: P. pacifica var. delicatula, P. pacifica var. distans, P. pacifica var. determinata, P. pacifica var. disticha, and P. pacifica var. gracilis. We here report morphology and phylogeny of P. pacifica to confirm the relationships among previously described varieties as a loan of type specimens from US and to assess phylogenetic relationships of closely related species using plastid protein-coding rbcL gene. Polysiphonia pacifica is distinguished by having creeping filaments attached by unicellular rhizoids not cut off by cross walls, four pericentral cells, ecorticate, trichoblasts rare, ultimate branchlets attenuate at the tip but not pungent, and tetrasporangia in long straight series in the ultimate branchlets. The protein-coding plastid rbcL gene sequence data show that P. pacifica is distinctly different from the superficially similar species, P. morrowii and P. stricta. However, the rbcL sequences of P. pacifica var. pacifica and var. disticha are identical though they have morphological variation.

• 환경위생공학
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• 제19권2호
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• pp.37-44
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• 2004

Flora and standing crops of the periphyton communities were examined at five sites in the mountainous streams of the Dam of Peace, Korea, from June to November 2003. The attached algal communities comprised totally 76 taxa belong to the 5 classes. In species composition, diatoms and green algae overwhelmingly dominated the periphyton communities although their standing crops were low. During the survey period, major dominant species were Navicula gregaria and Nav. pupla var. capitata, as 11.7% and 8.9% of total standing crop, respectively. Other subdominant species were Scenedesmus ecornis and Spirogyra weberii in green algae, Cymbella affinis, Cymbella minuta. Fragilaria capucina, Melosira varians, Naw. cryptocephala, Nav. exigua var. capitata, Nav. gregaria, Nit. sublineuris in diatom. In the seasonal appearances, S. ecornis and Oscillatoria nigra were occurred in summer, C. affinis and F. capucina in autumn, and N. sublinearis, and species Spirogyra weberii in winter, respectively.

• ALGAE
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• 제18권4호
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• pp.333-339
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• 2003

To establish a cultivation method of Petalonia fascia, seeds and seedlings cultures and growth tests were performed at the Daeri aquafarm in Haeui, Shinan, Jeollanamdo, Korea. Gametes were easily released from the mature plurilocular sporangia. They developed to crustose discoidal stolons and grew to filamentous and discoidal stolons. The indoor seeding was performed by using the 100-150 ${\mu}m$long fragments of stolons on Porphyra nets and the erect thalli developed from the cuttings when the seawater temperatures were 10-15$^{\circ}C$. In the experimental cultivation in the sea, 1-2mm long plantlets were found after 15 days of cultivation; after two months thalli grew to their maximal size of 215-355 mm long blades; after three months the length of thalli began to decrease due to distal disintegration and the plant color changed to yellow and epiphytic diatoms were attached on the thalli, which deteriorated the quality of products. The cultivation of P. fascia by the regeneration of filamentous-discoidal complexes was carried out successfully for the first time in Korea.

• 한국환경생태학회지
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• 제35권6호
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• pp.601-608
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• 2021

점몰개(Squalidus multimacultus)의 생태적 특징을 연구하기 위해 2020년 1월부터 12월까지 태화강 지류 척과천에서 조사를 실시하였다. 본 종의 서식지 하상구조는 모래(sand)가 매우 풍부하였으며, 수심은 25~164cm이었고 평균 68cm로 다소 깊었으며, 유속이 0.21±0.26(0.16~0.43)m/sec로 느렸다. 암수 성비는 1 : 0.99이었다. 전장빈도분포도에 따른 연령은 여름(6-7월)기준으로 50mm 미만(29.4~49mm)은 만1년생, 50~69mm은 만 2년생, 70~89mm은 만 3년생, 90mm 이상은 만 4년생으로 추정되었다. 생식가능 전장의 크기는 암컷과 수컷 모두 40mm 이상 되면 성적 성숙이 이루어졌다. 산란시기는 7월에서 8월(산란성기 8월)이었며 수온은 23.8~25.4℃이었다. 포란수는 평균 1,395(648~2,201)개 이었고 성숙란은 노란색 구형으로 직경이 0.67±0.24(0.62~0.83)mm이었다. 먹이생물은 부착조류(Attached algae), 동물플랑크톤(Zooplankton), 수서곤충(Aquatic insects), 연체동물(Mullusca) 등이었다. 식성은 잡식성이었으나 섭취한 먹이의 양이 95% 이상은 부착조류로 식물성 먹이를 섭취하고 있었다. 점몰개의 크기가 클수록 동물성 먹이인 수서곤충을 섭식하는 양과 섭식 빈도가 증가하였으며 수서곤충을 섭식한 개체는 식물성 먹이를 거의 섭취하지 않았다.