Hirata, T.;Tsutsui, C.;Yokoi, Y.;Sakatani, Y.;Mori, A.;Horii, A.;Yamamoto, T.;Taguchi, A.
Proceedings of the Korean Vacuum Society Conference
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2010.02a
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pp.44-45
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2010
We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.
An algorithm was developed to retrieve both cloud optical thickness and effective particle radius considered the aerosol effect on clouds. This study apply the algorithm of Nakajima and Nakajima (1995) that is used to retrieve cloud optical thickness and effective particle radius from visible, near infrared satellite spectral measurements. To retrieve cloud properties, Look-up table (LUT) was made under different atmospheric conditions by using a radiative transfer model. Especially the vertical distribution of aerosol is based on a tropospheric aerosol profile in radiative transfer model. In the case study, we selected the extensive forest fire occurred in Russia in May 2003. The aerosol released from this fire may be transported to Korea. Cloud properties obtained from these distinct atmospheric situations are analysed in terms of their possible changes due to the interactions of the clouds with the aerosol particle plumes. Cloud properties over the East sea at this time was retrieved using new algorithm. The algorithm is applied to measurements from the MODerate Resolution Imaging Spectrometer (MODIS) onboard the Terra spacecrafts. As a result, cloud effective particle radius was decreased and cloud optical thickness was increased during aerosol event. Specially, cloud effective particle radius is hardly greater than $20{\mu}m$ when aerosol particles were present over the East Sea. Clouds developing in the aerosol event tend to have more numerous but smaller droplets.
Although hyperspectral sensing data have shown great potential to derive various surface information that is not usually available from conventional multispectral image, the acquisition of proper hyperspectral image data are often limited. To use ground-based hyperspectral camera image for remote sensing studies, radiometric calibration should be prerequisite. The objective of this study is to develop radiometric calibration procedure to convert image digital number (DN) value to surface reflectance for the 120 bands ground-based hyperspectral camera. Hyperspectral image and spectral measurements were simultaneously obtained from the experimental target that includes 22 different surface materials of diverse spectral characteristics at wavelength range between 400 to 900 nm. Calibration coefficients to convert image DN value to at-sensor radiance were initially derived from the regression equations between the sample image and spectral measurements using ASD spectroradiometer. Assuming that there is no atmospheric effects when the image acquisition and spectral measurements were made at very close distance in ground, we were also able to derive calibration coefficients that directly transform DN value to surface reflectance. However, these coefficients for deriving reflectance values should not be applied when the camera is used for aerial image that contains significant effect from atmosphere and further atmospheric correction procedure is required in such case.
The estimation technique of surface water temperature by satellite remote sensing has been applied to ocean and large lakes using AVHRR. However, the spatial resolution AVHBR is not abquate for coastal region and small lakes. Landsat 5 TM has 120 m spatial resolution, which suits better. We carried out analysis of surface water temperature in Lake Sihwa and near coastal area using Landsat 5 TM. To relate digital number to the brightness temperature, we applied Empirical, NASA, RESTEC, Quadratic methods. Comparing calculated and observed value, we obtained as follows; NASA method, $R^2=0.9343$, RMSE(Root Mean Square Error)=3.5876$^{\circ}C$; RESTEC method, $R^2=0.8937$, RMSE=3.76$^{\circ}C$; Quadratic method, $R^2=0.8967$, RMSE=2.949$^{\circ}C$. Because Landsat TM has only one band for extracting surface temperature, it was difficult to correct for the atmospheric errors. For improving the accuracy of surface temperature detection using Landsat TM, there is a need for a method to decrease the effect of atmospheric contents.
Purpose: The atmospheric pressure plasma jet (APPJ) has been introduced as an effective disinfection method for titanium surfaces due to their massive radical generation at low temperatures. Helium (He) has been widely applied as a discharge gas in APPJ due to its bactericidal effects and was proven to be effective in our previous study. This study aimed to evaluate the safety and effects of He-APPJ application at both the cell and tissue levels. Methods: Cellular-level responses were examined using human gingival fibroblasts and osteoblasts (MC3T3-E1 cells). He-APPJ was administered to the cells in the experimental group, while the control group received only He-gas treatment. Immediate cell responses and recovery after He-APPJ treatment were examined in both cell groups. The effect of He-APPJ on osteogenic differentiation was evaluated via an alkaline phosphatase activity assay. In vivo, He-APPJ treatment was administered to rat calvarial bone and the adjacent periosteum, and samples were harvested for histological examination. Results: He-APPJ treatment for 5 minutes induced irreversible effects in both human gingival fibroblasts and osteoblasts in vitro. Immediate cell detachment of human gingival fibroblasts and osteoblasts was shown regardless of treatment time. However, the detached areas in the groups treated for 1 or 3 minutes were completely repopulated within 7 days. Alkaline phosphatase activity was not influenced by 1 or 3 minutes of plasma treatment, but was significantly lower in the 5 minute-treated group (P=0.002). In vivo, He-APPJ treatment was administered to rat calvaria and periosteum for 1 or 3 minutes. No pathogenic changes occurred at 7 days after He-APPJ treatment in the He-APPJ-treated group compared to the control group (He gas only). Conclusions: Direct He-APPJ treatment for up to 3 minutes showed no harmful effects at either the cell or tissue level.
This study investigated the effects of the urban forest and street trees on flow and temperature distribution in the Daegu National Debt Redemption Movement Memorial Park. For this, we implemented tree-drag and tree-cooling parameterization schemes in a computational fluid dynamics (CFD) model and validated the simulated wind speeds, wind directions, and air temperatures against the measured ones. We used the wind speeds, wind directions, air temperatures predicted by the local data assimilation and prediction system (LDAPS) as the inflow boundary conditions. To investigate the flow and thermal characteristics in the presence of trees in the target area, we conducted numerical experiments in the absence and presence of trees. In the absence of trees, strong winds and monotonous flows were formed inside the park, because there were no obstacles inducing friction. The temperature was inversely proportional to the wind speed. In the presence of trees, the wind speeds(temperatures) were reduced by more than 40 (5)% inside the park with a high planting density due to the tree drag (cooling) effect, and those also affected the wind speeds and temperatures outside the park. Even near the roadside, the wind speeds and temperatures were generally reduced by the trees, but the wind speeds and air temperatures increased partly due to the change in the flow pattern caused by tree drag.
Yun-Kyu Lim;Ki-Ho Chang;Yonghun Ro;Jung Mo Ku;Sanghee Chae;Hae-Jung Koo;Min-Hoo Kim;Dong-Oh Park;Woonseon Jung;Kwangjae Lee;Sun Hee Kim;Joo Wan Cha;Yong Hee Lee
Journal of Environmental Science International
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v.32
no.12
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pp.899-914
/
2023
Various seeding materials for cloud seeding are being used, and sodium chloride powder is one of them, which is commonly used. This study analyzed the experimental results of multi-aircraft cloud seeding in connection with Republic of Korea Air Force (CN235) and KMA/NIMS(Korea Meteorological Administration/National Institute of Meteorological Sciences) Atmospheric Research Aircraft. Powdered sodium chloride was used in CN235 for the first time in South Korea. The analysis of the cloud particle size distributions and radar reflectivity before and after cloud seeding showed that the growth efficiency of powdery seeding material in the cloud is slightly higher than that of hygroscopic flare composition in the distribution of number concentrations by cloud aerosol particle diameter (10 ~ 1000 ㎛). Considering the radar reflectivity, precipitation, and numerical model simulation, the enhanced precipitation due to cloud seeding was calculated to be a maximum of 3.7 mm for 6 hours. The simulated seeding effect area was about 3,695 km2, which corresponds to 13,634,550 tons of water. In the precipitation component analysis, as a direct verification method, the ion equivalent concentrations (Na+, Cl-, Ca2+) of the seeding material at the Bukgangneung site were found to be about 1000 times higher than those of other non-affected areas between about 1 and 2 hours after seeding. This study suggests the possibility of continuous multi-aircraft cloud seeding experiments to accumulate and increase the amount of precipitation enhancement.
This study was aimed to investigate the effects of restaurant in-store color and music congruency on consumer's emotional responses and behavioral intentions. The web survey was conducted among 400 customers(aged from 20~39 years old) who lived in Seoul and Kyunggi, Incheon Province. To find ensemble effect of color and music, 3D studio MAX were used to make high-stimulus(exciting) and low-stimulus(calm) and 3D virtual reality restaurant simulation stimulus were applied. The statistical data analyses were performed using SPSS/WIN 18.0 and reliability analysis, factor analysis, regression analysis were used. Based on the result of the conducting factor analysis, emotional responses were classified into 2 factors: positive emotion and negative emotion. Satisfaction was classified into 1 factor: satisfaction. Loyalty was classified into 1 factor: loyalty. Cronbach's alpha was calculated for the reliability of the survey instrument. Consequently, restaurant in-store color and music congruency were shown to affect positive emotion and negative emotion. Positive emotion and negative emotion were shown to affect satisfaction. Satisfaction were shown to affect loyalty. Music congruency had a higher effect on positive emotion than color congruency. Color congruency had a higher effect on negative emotion than music congruency. The results of this study will serve as a basis of color and music congruency with restaurant atmospherics.
Journal of the Korea institute for structural maintenance and inspection
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v.21
no.6
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pp.83-90
/
2017
Concrete structure for nuclear power plant is mass concrete structure with large wall depth and easily permits cracking in early age due to hydration heat and drying shrinkage. It always needs cooling water so that usually located near to sea shore. The crack on concrete surface permits rapid chloride intrusion and also causes more rapid corrosion in the steel. In the study, the effect of age and crack width on chloride diffusion is evaluated for the concrete for nuclear power plant with 6000 psi strength. For the work, various crack widths with 0.0~1.4 mm are induced and accelerated diffusion test is performed for concrete with 56 days, 180days, and 365 days. With increasing crack width over 1.0mm, diffusion coefficient is enlarged to 2.7~3.1 times and significant reduction of diffusion is evaluated due to age effect. Furthermore, apparent diffusion coefficient and surface chloride content are evaluated for the concrete with various crack width exposed to atmospheric zone with salt spraying at the age of 180 days. The results are also analyzed with those from accelerated diffusion test.
Atmospheric pressure plasma (APP) was applied to examine microbial safety and qualities of commercial Sunsik. APP was generated in a square-shaped plastic container (250 W, 15 kHz, ambient air) and dielectric barrier discharge plasma treatment was applied for periods of 0, 5, 10, and 20 minutes. The total aerobic bacterial count in the control was 4.44 log CFU/g. Under plasma treatment for 20 minutes, Sunsik samples inoculated with Bacillus cereus, B. subtilis, and Escherichia coli O157:H7 resulted in a reduction of bacterial counts by approximately 2.20, 2.22, and 2.52 log CFU/g, respectively. The pH of the sample was found to decrease after APP treatment. Although hunter color $L^*$ of Sunsik increased, $a^*$ and $b^*$ value decreased as a result of APP. Increasing the APP time also enhanced the peroxide value. Further, sensory evaluation revealed that APP decreased color, flavor, taste and overall acceptability. The results of this study indicated that APP treatment improved the microbial quality of Sunsik, although further studies should be conducted to reduce the deterioration of sensory quality induced by APP.
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