• Journal of Plant Biology
• /
• 제26권2호
• /
• pp.73-90
• /
• 1983

The analysis in external morphology of Astilbe including three endemic species in Korea was undertaken. From this study, five species and three varieties of Astilbe in Korea were redescribed. Their scientific names and Korean names were also consulted. Korean Astilbe could be grouped in two Series-Series 1. Simplicifoliae; Astilbe simplicifoliae and Series 2. compositae; A. taquetii, A. microphylla, A. chiensis var. chinensis, A. chinensis var. paniculata, A. chinensis var. davidii, A. divaricata, A. koreana. Interspecific relationships of eight taxa were represented as a toxonomic model by the considering their external morphology-pubescence on floral axis and surface of leafblade, inflorescence type, ratio of petal and stamen length-and distributional range in Korea and neighboring countries.

• 생약학회지
• /
• 제18권4호
• /
• pp.207-209
• /
• 1987

Bergenin was isolated from the ethylacetate fraction of the rhizomes of Astilbe divaricata. The structure of bergenin was identified by IR, UV, NMR, mass spectra and compared with the authentic sample.

• 식물분류학회지
• /
• 제39권1호
• /
• pp.35-41
• /
• 2009

노루오줌속(Astilbe)은 동아시아와 북미 동부에 격리되어 분포하는 양상을 보이는 속으로 잘 알려져 있다. 본 연구에서는 노루오줌속 및 근연 속을 대표하는 17종의 핵 리보솜 DNA의 ITS 염기서열에 기초한 계통수를 제작하여 노루오줌속의 분류, 형질 진화 및 계통지리에 대하여 고찰하였다. 베이스 추론법 등을 통해 계통수를 제작한 결과 Saxifragopsis가 노루오줌속의 자매군임이 확인되었고, 단성화, 무화피화, 7~11개로 갈라지는 꽃받침 등의 형질상태를 지니는 것으로 알려진 일본 고유종, A. platyphylla는 노루오줌속 계통수의 기부에서 가장 먼저 분리되었다. 북미에 격리 분포하는 분류군인 A. biternata 및 중국과 동남아시아에 분포하는 A. rivularis는 노루오줌속의 진화 초기에 갈라진 것으로 밝혀졌다. 한편, 화피가 있는 나머지 종들은 하나의 핵심군으로 확고하게 무리를 지었다. 핵심군 내에서는 일본, 대만, 필리핀 등지에 분포하는 종들("Japonica" clade) 이 단계통군을 이루었고, 중국 및 한국에 분포하는 종인 A. rubra var. rubra(노루오줌)과 A. koreana(숙은노루오줌), 즉 "Rubra" clade는 이들과 명확히 구분되는 진화 계열로 나타났다. A. biternata의 기원과 격리분포는 베링육교(Bering land bridges)를 통해 이루어진 것으로 추정되었다. 대만에 분포하는 2종과 A. philippinensis는 일본에 분포하는 분류군이 남하하면서 갈라져 나온 것으로 해석되었다. 노루오줌속 내에서 무화피화는 원시적 형질상태였으며, 이 형질상태는 적어도 두 개의 진화 계열에서 각각 독립적으로 기원하였을 것으로 추정되었다. 본 연구결과는 노루오줌속을 분류함에 있어서 잎의 유형(단엽/복엽)을 중요시했던 Engler의 분류 체계를 지지하지 않았으며, 꽃받침의 형태를 일차적으로 고려했던 Hara의 분류 견해를 지지하였다.

• Natural Product Sciences
• /
• 제9권4호
• /
• pp.226-231
• /
• 2003

Reactive oxygen species damage biomolecules such as lipids, proteins, sugars and DNA, which can not only lead to various disease but also oxidative damage resulting aging. In order to search for antioxidants from plants, the antioxidant effects of the MeOH extracts from 182 plants were evaluated. The results showed that thirteen plant extracts exhibited antioxidant activity (>80%) in DPPH radical assay, seven plant extracts demonstrated antioxidant activity (>40%) in the hydroxyl radical assay and eighteen plant extracts were active (>80%) in the lipid peroxidation assay. In particular, the extracts of Distylium racemosum (Hamamelidaceae), Astilbe koreana (Saxifragaceae), Astilbe chinensis and Euphorbia supina (Euphorbiaceae) were identified as potent principles of antioxidant activity in all the assay systems.

• 생약학회지
• /
• 제49권1호
• /
• pp.47-54
• /
• 2018

Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by $^1H$, $^{13}C$ NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. $500{\mu}g/mL$ EAC treatment inhibited Hela cell at $60.2{\pm}1.5%$.

• 약학회지
• /
• 제36권5호
• /
• pp.474-480
• /
• 1992

Astilbes rhizoma has been used for headache, arthralgia, chronic bronchitis and stomachalgia in traditional chinese medicine. The analgesic activities and their components of Astilbe chinensis var. davidii Rhizomes were evaluated. The ether and ethylacetate fractions of 70% EtOH extract showed considerable analgesic activities by acetic acid induced writhing method. Compound $1{\sim}5$ were isolated from ethylacetate fraction and identified as gallic acid, (+)-catechin, (+)-gallocatechin, bergenin and 11-O-galloylbergenin on the basis of spectroscopic methods. Among them (+)-gallocatechin showed stronger analgesic activity than that of other compounds.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 춘계학술대회
• /
• pp.102-102
• /
• 2019

This study developed a HPLC analysis method for the determination of avicularin (AL) and quercitrin (QT) in Astilbe chinensis by HPLC. HPLC was performed on a Capcell Pak C18 MGII column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$ and a UV detection wavelength of 254 nm. The analytical method was validated for its specificity, precision, accuracy, and linearity. The limits of detection and quantitation were AL 0.09 and 0.28 mg/mL, QT 0.03 and 0.09 mg/mL respectively. Calibration curves showed good linearity (AL r2 > 0.99990 and QT r2 > 0.99994), and the precision of analysis was satisfied (less than AL 0.59%, QT 0.63%). Recoveries of quantified compounds ranged from AL 100.57 to 102.08%, QT 99.94 to 100.64%. These results indicate that the developed HPLC method was applied successfully to the detection of AL and QT in A. chinensis.

• 조경수
• /
• 통권93호
• /
• pp.12-14
• /
• 2006

• 대한화장품학회지
• /
• 제45권4호
• /
• pp.333-340
• /
• 2019

본 연구는 노루오줌(Astilbe chinensis) 추출물을 기능성 화장품소재로 개발하기 위해 avicularin, quercitrin을 지표성분으로 선정하고, 품질관리를 위해 high performance liquid chromatography (HPLC)를 이용하여 분석법을 개발하였다. 분석용 칼럼은 Capcell Pak C₁₈ MGII을, 이동상은 0.05% (v/v) trifluoroacetic acid (TFA)와 아세토니트릴을 사용하여 컬럼 온도는 30℃ 에서 유속 1.0 mL/min 로 검출파장은 254 nm에서 검출하였다. International conference on harmonization (ICH) 가이드라인(version 4, 2005)을 근거로 하여 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계를 분석하여 분석방법을 검증하였다. 분석결과, 검출한계 및 정량한계는 avicularin 0.094 및 0.285 mg/mL, quercitrin 0.031 및 0.095 mg/mL로 나타났으며, 검량곡선은 상관계수 값이 avicularin 0.99990, quercitrin 0.99994로 양호한 직선성을 보였고 정밀성 분석결과도 avicularin 0.59%, quercitrin 0.63% 이하로 확인되었다. 또한, 회수율은 avicularin 100.97 ~ 101.77%, quercitrin 100.18 ~ 100.32% 범위로 정확성이 있음을 알 수 있다. 따라서, 본 분석법은 노루오줌 추출물 지표성분의 분석법으로 적합한 시험법임이 검증되었다.

• 한국약용작물학회지
• /
• 제26권4호
• /
• pp.281-290
• /
• 2018

Background: Lung cancer, the most common malignant disease worldwide, is the predominant cause of cancer deaths, particularly amongst men. Therefore, various researchers have focused on the growth inhibitory effects of medicinal plants used in traditional Korean medicine. This study aimed to investigate the growth inhibitory effects of ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos on NCI-H1229 cells. Method and Results: The viability of NCI-H1229 cells was evaluated in vitro using an MTS assay. Treatment with the ethanol extracts of the selected medicinal plants at $500{\mu}g/m{\ell}$ reduced NCI-H1229 cell viability and increased apoptotic cell death and caspase-3 activation. In addition, treatment with ethanol extracts of Inulae flos and Astilbe radix increases DNA fragmentation, as measured by the TUNEL assay. Conclusions: These results indicated that ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos exhibited growth inhibitory effects, inducing apoptotic cell death, DNA fragmentation and caspase-3 activation in NCI-H1229 cells. Therefore, these medicinal plant extracts may be used in the development of natural medicines to inhibit the growth of lung cancers. However, further study is needed to determine the active ingredients of the ethanol extracts from medicinal plants that are reposible for the inhibitory effect on lung cancer cell grwoth.