• 한국가금학회지
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• 제35권3호
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• pp.219-224
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• 2008

63주령 ISA Brown 산란계 총 96수를 사용하여, 12일 동안 난황에서의 biological astaxanthin과 chemical astaxanthin 및 capxanthin의 축적을 통한 착색 효과를 비교하였다. 대조구 사료에는 착색제를 첨가하지 않았고, 효모 Phaffia rhodozyma 3%를 대조구 사료에 첨가하여 astaxanthin 함량이 22.5 mg/kg 되게 한 후, 화학적으로 합성한 astaxanthin을 대조구 사료에 45 mg/kg 첨가한 후 및 paprika로부터 추출한 capxanthin을 대조구 사료에 45 mg/kg 첨가구 모두 4개의 처리구들을 두었다. 처리당 6반복, 반복당 4수씩을 임의로 배치하였다. Astaxanthin은 생물학적 및 화학적 급원 모두 급여 9일째까지 축적이 이루어졌다. Capxanthin의 경우, 난황에서 축적되지는 않았고 이로부터 파생되어진 물질들이 급여 $6{\sim}9$일 후에 미미하게 검출되었다. 난황에서의 astaxanthin 축적 정도는 급여한 농도에 비례하였다. 난황의 색깔을 제외하면, 달걀의 다른 품질 요인들은 처리구들 간의 차이가 거의 없었다.

• 한국식품영양과학회지
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• 제27권1호
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• pp.163-167
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• 1998

Anticarcinogenic activity of astaxanthin-contatining egg yolk(designate AEY) was investigated for mouse ascites carcinogenesis induced by mouse Sarcoma-180(S-180) cells. Female ICR mice(8mice/treatment, 7∼8weeks of age, 25±1g) were injected, i.p. with S-180 cells(1×107cell/ml PBS). Two days later, each mouse was given 0.1ml PBS containing AEY(10, 25 or 50ug/g body weight) or control egg yolk (CEY; 50ug/g body weight) every other day for 7 times. Control mice were only given 0.1ml S-180 cells and 0.1ml PBS. Mice treated with 25ug/g body weight of AEY showed 24.8 days of life, which was equivalent to 138% of control mice's life(180.0 dyas). Based on dose-dependant experiment of AFY, mice treated with 10ug/g body weight showed slightly longer life(19.4 days) relative to mice treated with control mice, and mice treated with 50ug/g body weight exhibited 21.9 days of life. Mice treated with any dose of AEY exhibited longer life than mice with CEY 50ug/g body weight. Body weight of mice treated with AEY was reduced relative to that of control mice CEY-treated mice. These results suggest than AEY inhibits the carcinogenesis of mouse ascites induced by S-180 cells.

• 한국환경성돌연변이발암원학회지
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• 제18권1호
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• pp.22-25
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• 1998

Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for 7,12-dimethylbenz[a]anthracene (DMBA)-induced two stage mouse epidermal carcinogenesis. Female ICR mouse (6-7 weeks of age) were house in a humidity-and-temperature-controlled facility and subjected to feed and water ad libitum. AEY (10 mg/0.2 ml acetone) was painted on the back of mice 7 days, 3 days and 5 min before DMBA treatment (50 nmole/0.2 ml acetone). One week later after DMBA treatment, 6 ${\mu}g$ tetradecanoyl 12-phorbol 13-O-acetate (TPA) dissolved in 0.2 ml acetone was applied on the mouse twice weekly over a period of 22 weeks. No sample was given to control mice. Control egg yolk (CEY) and astaxanthin-containing oil (designate AO) from Phaffia rhodozyma were used as positive controls. Mouse treated with AEY exhibited 10 tumors per mouse whereas control mouse exhibited 15 tumors per mouse, the fact that 33% reduction of tumor per mouse by AEY treatment. Tumor incidence was also reduced to 15% by AEY treatment when compared to that of control group. Such effects were also seen in CEY and AO treatment groups, but leaser extent. AO gave reduction of food intake and body weights relative to those of AEY and CEY, indicating toxicity of AO. These results suggest that AEY exhibits anticarcinogenic activity for DMBA-induced mouse epidermal carcinogenesis.

• Animal Bioscience
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• 제34권3_spc호
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• pp.443-448
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• 2021

Objective: Astaxanthin is a natural super antioxidant. The present study was carried out to investigate the effect of astaxanthin rich Phaffia rhodozyma (PR) supplementation in diets on laying production performance, egg quality, antioxidant defenses and immune defenses in laying hens. Methods: A total of five hundred and twelve 60-week-old Lohmann Brown laying hens (2,243±12 g) were randomly assigned to four groups, each including 4 replicates with 32 birds per replicate. Astaxanthin rich PR was added to corn-soybean meal diets to produce experimental diets containing 0 (Control), 800 mg/kg, 1,200 mg/kg, and 1,600 mg/kg PR, respectively. The astaxanthin content in the diet was 0.96 mg/kg, 1.44 mg/kg and 1.92 mg/kg respectively. Results: Results showed that dietary PR supplementation tended to increase daily feed intake (p = 0.0512). There was no effect of astaxanthin rich PR on Haugh units, albumen height, egg shape index, eggshell strength, and eggshell thickness at weeks 6 (p>0.05). However, egg yolk color was significantly improved (p<0.05). In addition, astaxanthin rich PR supplementation significantly increased serum glutathione peroxidase and superoxide dismutase activity (p<0.05), increased serum immunoglobulin G content (p<0.05), and reduced malondialdehyde content (p<0.05) in laying hens. Conclusion: In conclusion, astaxanthin rich PR can improve the color of egg yolk, enhance the antioxidant defenses, and regulate the immune function.

• Applied Biological Chemistry
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• 제40권6호
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• pp.490-494
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• 1997

Benzo[a]pyrene (BP)으로 유발한 mouse의 전위암 형성에 대한 astaxanthin 함유 난황 (astaxanthin-containing egg yolk : AEY)의 영향을 연구하였다. Female ICR mouse (6-7 주령, 5 mice/cage, 20 mice/treatment)에게 물과 사료를 자유로이 공급하면서 일주일간 적응시킨 후 다음과 같이 처리를 하였다. BP (2 mg/0.2 ml corn oil)를 각 mouse에 경구투여하기 4 일과 2 일 전에 50 mg AEY, 100 mg AEY, 150 mg AEY, 또는 150 mg control egg yolk (CEY)을 함유하는 phosphate-buffered saline (PBS) 0.2 ml를 경구투여하였다. Control mouse는 0.2 ml PBS와 BP 만 경구투여하였다. 이 과정을 4회 반복하였다. BP를 경구투여 한 일주일 후부터 몸무게와 사료섭취량을 매주 기록하였으며 24주 후에 생존한 모든 mouse에 대해 전위암 생성을 조사하였다. AEY를 처리한 mouse에서는 control mouse나 CEY 처리 mouse에 비해 mouse 당 암의 수가 1/3 정도로 감소되었다. 이와 같은 AEY의 항암효과는 AEY의 처리량에 비례하였다. AEY 처리에 따른 암발생율은 control이나 CEY 처리에 비해 감소되었으나 150 mg AEY 처리에서만 유의성이 있는 감소를 보였다. AEY 처리에 따른 몸무게나 사료 소비량의 감소현상은 볼 수 없었다. 따라서 이 결과는 AEY가 BP로 유발한 mouse의 전위암 발생을 억제함을 암시한다.