• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.554-559
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• 1999

The purpose of this study was to evaluate effect of chitosan-oligosaccharides (CHIOL) on hydrophobicity of pathogenic E coli including a field isolate from suckling piglet with diarrhea, E coli-O157 : H7, and E coli-O149 : K88ac. E coli field isolate appeared adhesion of 100% to n-hexadecane between 0.00125% and 0.05% CHIOL. E coli-O157 : H7 occurred adhesion of 69% and 64% under the level of 0.00125% and 0.025% CHIOL, respectively. E coli-O149 : K88ac showed adhesion of 100% in higher than 0.025% CHIOL. For cationic action, the adhesion of E coli isolate and E coli-O149 : K88ac to n-hexadecane were inhibited at level of higher than 10mM $Ca^{2+}$ but did not induce any difference among the concentrations used(p < 0.01). However, the adhesion of E coli-O157 : H7 to n-hexadecane was inhibited at level of higher than 50mM $Ca^{2+}$. In a field trial, control piglets showed average mortality of up to 58% during 3 days after the onset of diarrhea. In contrast, the prevalence of E coli-induced diarrhea in CHIOL-treated groups without mortality was dropped down to average 34% on the 1st day after the treatment of CHIOL, and average 2% on the 4th day. After then, piglets with diarrhea was not present. In conclusion, the low concentrations of CHIOL were most likely to associate with the enhancement of hydrophobicity to pathogenic E coli. Calcium inhibited the hydrophobicity of E coli by CHIOL. These results suggested that CHIOL could be played an efficient and reliable role in treating enteric colibacillosis of piglets.

• Molecules and Cells
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• v.38 no.4
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• pp.349-355
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• 2015

EphA7 has been implicated in the regulation of apoptotic cell death in neural epithelial cells. In this report, we provide evidence that EphA7 interacts with caspase-8 to induce apoptotic cell signaling. First, a pull-down assay using biotinylated ephrinA5-Fc showed that EphA7 co-precipitated with wild type caspase-8 or catalytically inactive caspase-8 mutant. Second, co-transfection of EphA7 with caspase-8 significantly increased the number of cleaved caspase-3 positive apoptotic cells under an experimental condition where transfection of EphA7 or caspase-8 alone did not affect cell viability or apoptosis. EphA4 also had a causative role in inducing apoptotic cell death with caspase-8, whereas EphA8 did not. Third, caspase-8 catalytic activity was essential for the apoptotic signaling cascade, whereas tyrosine kinase activity of the EphA4 receptor was not. Interestingly, we found that kinase-inactive EphA4 was well co-localized at the plasma membrane with catalytically inactive caspase-8, suggesting that an interaction between these mutant proteins was more stable. Finally, we observed that the extracellular region of the EphA7 receptor was critical for interacting with caspase-8, whereas the cytoplasmic region of EphA7 was not. Therefore, we propose that Eph receptors physically associate with a transmembrane protein to form an apoptotic signaling complex and that this unidentified receptor-like protein acts as a biochemical linker between the Eph receptor and caspase-8.

• Journal of Korean Society for Geospatial Information Science
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• v.1 no.1 s.1
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• pp.171-180
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• 1993

Concern about nonpoint source pollution associated with urban storm water has led to the development of new tools for better water quality planning. This paper presents an application of a geographic information system (GIS) for urban water quality study. The GIS was used to manage land use data for nonpoint source pollution modeling and to aggregate pollutant loadings within various types of geographic units. An empirical water quality model was used to estimate pollutant loadings based primarily on land use. A land use coverage was created by updating an old coverage through interpretation of recent photography. This land use coverage was also used to record all pollutant loadings for each land use polygon. Storm sewer maps were digitized and interpreted to create a coverage of storm sewer basins and sub-basins. By overlaying pollutant loadings with the sewer sub-basin layer, aggregated pollutant loadings for major sewer outfalls were calculated. Based on the loading information, critical areas of excessive pollutant loadings were located and the effectiveness of Best Management Practices (BMPs) to control pollutant loadings were evaluated.

• The Journal of the Korea Contents Association
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• v.14 no.9
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• pp.120-130
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• 2014

The purpose of this study was to evaluate the effects of line dance activity on the balance factors during static standing to reveal the exercise intervention for fall prevention. A 15-week line dance programme was applied to 16 elderly females who aged more than 65 years in the community. Balance ability during static standing was evaluated by the range of center of pressure(cop), the velocity of cop, and sway area that calculated on the basis of ground reaction force data, forces, and moments. The range and velocity of cop in the anterio-posterior were significantly reduced after line dance(p<.01, p<.05, respectively), but change in those of cop in the medio-lateral was not found. It was demonstrated that 16-week line dance activities allow more effective in anterio-posterior stability and sway area of static standing. It was suggested that the effect of fall prevention exercise should be studied more associate with fall from vestibular and sensory system as future study.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.1
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• pp.37-45
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• 2015

Recent droughts in South Korea have had large economic and environmental impacts across the country. Changes in rainfall and hydrologic patterns due to climate change can potentially increase the occurrence of extreme droughts and affect the future availability of water resources. Therefore, it is necessary to evaluate drought vulnerability for water resources planning and management, and identify the appropriate mitigation actions to conduct a drought risk analysis in the context of climate change. The objective of this study is changes in the temporal trends of drought characteristics in South Korea to examine drought impacts under climate change. First, the changes of drought occurrence were analyzed by applying the Standardized Precipitation Evapotranspiration Index (SPEI) for meteorological data on 54 meteorological stations, and were analyzed for the past 30 years (1981-2010), and Representative Concentration Pathways (RCP) climate change scenarios (2011-2100). Second, the changes on the temporal trends of drought characteristics were performed using run theory, which was used to compare drought duration, severity, and magnitude to allow for quantitative evaluations under past and future climate conditions. These results show the high influence of climate change on drought phenomenon, and will contribute to water resources management and drought countermeasures to climate change.

• Journal of the Korean Institute of Intelligent Systems
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• v.10 no.3
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• pp.218-227
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• 2000

In this paper, we present a new fuzzy information retrieval method based on thesaurus. In the proposed method th thesaurus is represented by a fuzzy linguistic matrix, where the elements in fuzzy linguistic matrix represent a qualitative linguistic values between terms. In the fuzzy linguistic matrix, there are three kinds of fuzzy relationships between terms, i.e., similar relation, hierarchical relation, and associative relation. The implicit fuzzy relationships between terms are inferred by the transitive closure of the fuzzy linguistic matrix based on fuzzy theory. And the proposed method has the capability to deal with a qualitative linguistic weights in a query and in indexing of information items to reflect qualitative measure of human based on vague and uncertain decisions rather than a quantitiative measure. Therefore the proposed method is more flexible than the ones presented in papers[1-3]. Moreover our method is more effectual of time than the ones presented in papers[1-3] because we use a fuzzy linguistic matrix and AON (Associate Ordinary Number) values in query evaluation process. As a result, the proposed method allows the users to perform fuzzy queries in a more flexible and more intelligent manner.

• Korean Journal of Community Nutrition
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• v.9 no.4
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• pp.519-527
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• 2004

This study was designed firstly to measure the job satisfaction (JS) levels of personnel engaged in contract food-service management company (CFMC), secondly to compare the job satisfaction levels by personal and company-related characteristics, and thirdly to identify the effects of JS facets on overall job satisfaction. Of 1135 respondents, there were 712 employees in the headquarters and 423 in the branch office. From an analysis on job satisfaction, overall job satisfaction level was 3.22 out of a maximum 5 and the co-worker (3.71) facet of JS was the highest job satisfaction followed by supervision (3.32), work itself (3.26), working condition (3.15), promotion (2.95) and payroll (2.74). In comparison of job satisfaction by personal characteristics, the personnel who were male (p < .01), had associate degrees (p < .01) or long-term careers in foodservice field (p < .05), or were regular employees (p < .01) perceived significantly higher than others for overall JS. In comparison of the job satisfaction by company-related characteristics, overall JS was significant by company scale (p < .01) and by work place (p < .05), but it was not significant by operating group. Finally, on the regression analysis for the effects of JS facets on overall JS, adjusted R2 was 0.534 (p < .001) and all six JS facets, especially payroll, had a positive effect on overall JS significantly (p < .001). Considering that the goal of enterprise on profit-making through customer satisfaction (CS) and the role of personnel on CS at moment of thrust (MOT), the findings confirmed the necessity for continuous internal marketing and human relation management focusing on the lower level of JS facets.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.227-234
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• 2006

The comparison of Corylopsis coreana populations between natural groups and deforestation groups is carried in five regions such as Seungju, Woldeung, Hwangjeon in Suncheon, Myeongseok in Jinju and Mt. Baegun in Pocheon. The number of sprouting per both $100m^2$ and each individual stump were appeared more in deforestation than in natural groups. In contrast, the height and the diameter of individuals by the number of sprouting are higher and larger in the natural groups. The relationship between the floristic composition and vegetation in the two groups of C. coreana populations was not appeared. Corylopsis coreana are propagated sprouting as well as seedling in deforestation groups located an opening area. Because of the powerful environmental adaptability through growth of the sprouting and the seedling in deforestation groups, the population of C. coreana are not extinction of species oneself, and on the contrary are showed expansion of C. coreana population after temporary disturbance.

• Journal of the Earthquake Engineering Society of Korea
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• v.8 no.3
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• pp.43-52
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• 2004

This paper describes a method of evaluating seismic system performance of highway transportation network in California. The basic element that plays a crucial role in this study is the fragility information of highway bridges in Caltrans' (California Department of Transportation) freeway network. The bridge fragility information is expressed as a function of the ground motion intensity, such as peak ground acceleration (PGA) or peak ground velocity (PGV). Network damage was evaluated under the 1994 Northridge earthquake and scenario earthquakes. A probabilistic model was developed to determine the effect of repair of bridge damage on the improvement of the network performance as days passed after the event. As an example, the system performance degradation measured in terms of an index, “Drivers Delay”, is calculated for the Los Angeles area transportation system, and losses due to Drivers Delay with and without retrofit were estimated.

• Biomedical Science Letters
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• v.17 no.1
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• pp.7-12
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• 2011

Free-living amoebae ingest several kinds of bacteria. In other words, the bacteria can survive within free-living amoeba. To determine how Escherichia coli K1 isolate causing neonatal encephalitis and non-pathogenic K12 interact with free-living amoebae, e.g., Acanthamoeba castellanii (T1), A. astronyxis (T7), Naegleria fowleri, association, invasion and survival assays were performed. To understand pathogenicity of free-living amoebae, in vitro cytotoxicity assay were performed using murine macrophages. T1 destroyed macrophages about 64% but T7 did very few target cells. On the other hand, N. fowleri which needed other growth conditions rather than Acanthamoeba destroyed more than T1 as shown by lactate dehydrogenase (LDH) release assay. In association assays for E. coli binding to amoebae, the T7 exhibited significantly higher association with E. coli, compared with the T1 isolates (P<0.01). Interestingly, N. fowleri exhibited similar percentages of association as T1. Once E. coli bacteria attach or associate with free-living amoeba, they can penetrate into the amoebae. In invasion assays, the K1 (0.67%) within T1 was observed compared with K12 (0%). E. coli K1 and K12 exhibited high association with N. fowleri and bacterial CFU. To determine the fate of E. coli in long-term survival within free-living amoebae, intracellular survival assays were performed by incubating E. coli with free-living amoebae in PBS for 24 h. Intracellular E. coli K1 within T1 (2.5%) and T7 (1.8%) were recovered and grown, while K12 were not found. N. fowleri was not invaded and here it was not recovered.