• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.932-941
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• 2013

This work aimed to classify Aspergillus (8 species, 28 strains) by using a secondary metabolite profile-based chemotaxonomic classification technique. Secondary metabolites were analyzed by liquid chromatography ion-trap mass spectrometry (LC-IT-MS) and multivariate statistical analysis. Most strains were generally well separated from each section. A. lentulus was discriminated from the other seven species (A. fumigatus, A. fennelliae, A. niger, A. kawachii, A. flavus, A. oryzae, and A. sojae) with partial least-squares discriminate analysis (PLS-DA) with five discriminate metabolites, including 4,6-dihydroxymellein, fumigatin, 5,8-dihydroxy-9-octadecenoic acid, cyclopiazonic acid, and neosartorin. Among them, neosartorin was identified as an A. lentulus-specific compound that showed anticancer activity, as well as antibacterial effects on Staphylococcus epidermidis. This study showed that metabolite-based chemotaxonomic classification is an effective tool for the classification of Aspergillus spp. with species-specific activity.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.1
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• pp.15-21
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• 2016

Aspergillus is the most common opportunistic fungus causing infection. Aspergillus is the most morphologically identified in the laboratory. Recently, molecular genetic methods have been proposed for identification of fungi that unidentified morphologically or identified genus level. Of 475 cases of Aspergillus isolated from clinical specimens, there were Aspergillus fumigatus 257 (54.1%), A. niger 101 (21.3%), A. flavus 43 (9.1%), A. terreus 29 (6.1%), Aspergillus nidulans 2 (0.4%), Aspergillus clavatus 1 (0.2%), and the Aspergillus species 42 (8.8%). Eleven cases of unidentified or identified at the genus level included Aspergillus fumigatus 5, Aspergillus falvus 1, Aspergillus terreus 1, and Aspergillus lentulus 1 was identified in the sequencing of the strain level. It was identified as Aspergillus versicolor 2, and Emericella parvathecia 1. 92.2% of Aspergillus was identified as a possible morphological, 8.8% could not be identified at the species level. Sequence-based molecular analysis using the ITS and D1D2 is considered useful for identification of the species level.

• Mycobiology
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• v.38 no.1
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• pp.1-6
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• 2010

63 strains of Aspergillus section Fumigati were isolated from 17 samples of arable soil in a central province of Korea. Based on the results of genotypic and phenotypic analyses, they were identified as Aspergillus fumigatus, A. lentulus, Neosartorya coreana, N. fennelliae, N. fischeri, N. glabra, N. hiratsukae, N. laciniosa, N. pseudofischeri, N. quadricincta, N. spinosa and N. udagawae. Among these, N. fennelliae, N. hiratsukae, N. quadricincta, and N. udagawae had not been previously recorded in Korea. The diversity of Aspergillus section Fumigati species from arable soil in Korea is also addressed.