• Title/Summary/Keyword: Aspergillus fumigatus KC-1

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Microbial Conversion of Woody Waste into Sugars and Feedstuff (II) - Production of Cellulolytic Enzymes from Aspergillus fumigatus and Saccharification of Popla Wood (미생물(微生物)에 의한 목질자원(木質資源)의 당화(糖化) 및 사료화(飼料化)에 관(關)한 연구(硏究) (II) - Aspergillus fumigatus KC-1으로부터 섬유소 분해 효소의 생산 및 현사시나무의 효소가수분해)

  • Chung, Ki-Chul;Huh, Jeong-Weon;Myung, Kyu-Ho;Kim, Yoon-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.15 no.4
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    • pp.18-25
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    • 1987

  • The cellulolytic activities of Aspergillus fumigatus KC-1 was investigated, which showed the most active producer of cellulase among the 256 strains of cellulose-decomposing microorganisms screened in our laboratory. All the examined cellulolytic activities (filter paper-, Avicel-, cotton-, CMC-, salicin- and xylansaccharifying activity) in a culture of A. fumigatus KC-1 grown on 1% popular sawdust pretreated with peroxide alkaline reached a maximum within 4-5 days. The optimum pH and temperature for the enzymatic activity was found to be pH 4.5 and $60^{\circ}C$ respectively. The sawdust of poplar wood delignified with 1% NaOH and 20% peracetic acid succesively recorded the highest hydrolysis rate in the tests of enzymatic saccharification. The major end product of hydrolysis of poplar wood with the cellulolytic enzymes obtained from A. fumigatus KC-1 was glucose with small amount of cellobiose and xylose. It can be concluded from these results that A. fumigatus KC-1 is an advantagous source of a cellulase that is capable of hydrolyzing cellulose to glucose rapidly. The influence of degree of delignification, substrate size and its concentration on the rate of hydrolysis of poplar wood was also discussed.

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