• Title/Summary/Keyword: Aspergillus flavus

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Liquid Chromatography-Mass Spectrometry-Based Chemotaxonomic Classification of Aspergillus spp. and Evaluation of the Biological Activity of Its Unique Metabolite, Neosartorin

  • Lee, Mee Youn;Park, Hye Min;Son, Gun Hee;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.932-941
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    • 2013
  • This work aimed to classify Aspergillus (8 species, 28 strains) by using a secondary metabolite profile-based chemotaxonomic classification technique. Secondary metabolites were analyzed by liquid chromatography ion-trap mass spectrometry (LC-IT-MS) and multivariate statistical analysis. Most strains were generally well separated from each section. A. lentulus was discriminated from the other seven species (A. fumigatus, A. fennelliae, A. niger, A. kawachii, A. flavus, A. oryzae, and A. sojae) with partial least-squares discriminate analysis (PLS-DA) with five discriminate metabolites, including 4,6-dihydroxymellein, fumigatin, 5,8-dihydroxy-9-octadecenoic acid, cyclopiazonic acid, and neosartorin. Among them, neosartorin was identified as an A. lentulus-specific compound that showed anticancer activity, as well as antibacterial effects on Staphylococcus epidermidis. This study showed that metabolite-based chemotaxonomic classification is an effective tool for the classification of Aspergillus spp. with species-specific activity.

The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals (TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구)

  • 한양일;김광호;오영복
    • Journal of Environmental Health Sciences
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    • v.5 no.1
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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Effects of Plant Extracts on Conidial Germination, Mycelial Growth and Sporulation of Fungi Isolated From Poultry Feed

  • Islam, M. Rafiqul;Alam, Shahidul;Rahman, M. Ziaur;Chowdhury, S.P.;Begum, M.F.;Akhter, Nargis;Alam, M.S.;Han, Kee-Don;Lee, Min-Woong
    • Mycobiology
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    • v.31 no.4
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    • pp.221-225
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    • 2003
  • Effect of ethanolic extracts of Lawsonia inermis, Azadirachta indica, Vinca rosea, Tagetes patula, Ocimum sanctum, Colocasia antiquorum, Adhatoda vasica, Moringa oleifera, Datura metel and Curcuma longa leaf on conidial germination, mycelial growth and sporulation of Aspergillus flavus, A. niger and A. fumigatus were examined. The conidial germination of A. flavus and A. fumigatus were most inhibited by the extract of L. inermis, while that of A. niger was inhibited by A. indica. Other tested plant extracts have a good effect on conidial germination on the selected fungi. The highest mycelial growth of A. flavus(37 mm) was found in V. rosea, but in case of A. niger and A. fumigatus it(38 and 39 mm) was found in D. metel. The lowest(4, 9 and 6 mm) respectively mycelial growth of these fungi found in L. inermis. The highest sporulation($75{\times}10^4/ml$) of A. flavus was counted in V. rosea, but in case of A. niger and A. fumigatus those($45{\times}10^4$ and $55{\times}10^4/ml$) were in D. metel and the lowest($5{\times}10^4,\;12{\times}10^4\;and\;9{\times}10^4/ml$) respectively sporulation of these fungi counted in L. inermis plant extract medium.

Cellulase activity of Aspergilli distributed in South Korea(I) (한국산 Aspergilli의 셀룰라아제 활성에 관한 연구 1)

  • 이영녹;김낙정;서항원
    • Korean Journal of Microbiology
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    • v.14 no.3
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    • pp.105-116
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    • 1976
  • 410 strains which belong to the genus aspergillus were isolated from specimens collected throught South Korea, and 17 species nad 1 variety through the 13 species groups were identified according to Repaer-Fennell's classification key. Among them the species and variety unrecorded in Korea are as follows : A, giganiteus, A. pseudogloucus, A. spinulosus, A.ficuum, A.japonicus, A.flavus var. columnaris, A. flavipes.

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Electron Microscopic Studies on the Cellular Changes in the Liver of Ducklings Induced by Korean Aflatoxin B$_1$ (한국산 Aflatoxin이 오리새끼의 간장에서 유발시킨 병변에 관한 전자현미경적 연구)

  • Lee Kwang-Juing;Yoon Hwa-Juing;Lee In-ho
    • Journal of the korean veterinary medical association
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    • v.26 no.9
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    • pp.543-553
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    • 1990
  • This report describes the cellular changes induced in the livers of ducklings by a sin91e administration of aflatoxin B$_1$ produced by Korean Industrial Strain of the Aspergillus flavus, in order to examine the toxicity of the minimum dose of

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Studies on Mycotoxins in Feed (사료내의 곰팡이 독소에 관한 연구)

  • Yoon Hwa-Joong;Chang Kyoung-Jin;Kim Tae-Jong
    • Journal of the korean veterinary medical association
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    • v.19 no.7
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    • pp.67-73
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    • 1983
  • Strains of Aspergillus flavus produced aflatoxin $B_1$ was found in the corns for eed and cultured them in the incubator. The aflatoxin $B_1$ was produced from the Aspergilli and intubated into the pharynx of the ducklings for the to

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Mycoflora in Broiler Houses (Broiler양계사내(養鷄舍內)의 진균총(眞菌叢))

  • Choi, Won-pil;Yeo, Sang-geon;Lee, Hun-jun
    • Korean Journal of Veterinary Research
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    • v.24 no.1
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    • pp.36-39
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    • 1984
  • The present survey was undertaken to determine the mycoflora of broiler houses. Attempts were made to isolate and identify fungi in the dust, feed, litter and water from 21 broiler houses. A total of 166 isolates of fungi was identified as yeast spp. (44%), Aspergillus spp. (30.7%), Verticillium spp. (7.2%), Penicillium spp. (3.6%), Paecilomyces spp. (3.6%), Scopulariopsis spp. (3.0%), Cephalosporium spp. (3.0%), Chrysosporium spp. (2.4%), Cladosporium spp. (1.8%) and Absidia spp. (0.6%). Isolated of Aspergillus(A) spp. and Penicillium(P) spp. were identified as A. fumigatus, A. flavus, A. terreus, A. nidulans, A. niger, P. citrinum and P. palitan. Fungal contamination of the broiler houses predominated in the dust. The important point is that must of the isolates were pathogenic fungi.

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Antifungal Activities of Biorelevant Complexes of Copper(II) with Biosensitive Macrocyclic Ligands

  • Raman N.;Joseph J.;Velan A. Senthil Kumara;Pothiraj C.
    • Mycobiology
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    • v.34 no.4
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    • pp.214-218
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    • 2006
  • Four copper(II) complexes have been prepared using macrocyclic ligands. The macrocyclic ligands have been synthesized by the condensation reaction of diethyl phthalate with Schiff bases derived from o-phenylene diamine and Knoevenagel condensed ${\beta}-ketoanilides$ (obtained by the condensation of acetoacetanilide and substituted benzaldehydes). The ligands and copper complexes have been characterized on the basis of Microanalytical, Mass, UV-Vis., IR and CV spectral studies, as well as conductivity data. On the basis of spectral studies, a square-planar geometry for the copper complexes has been proposed. The in vitro antifungal activities of the compounds were tested against fungi such as Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans. All the synthesized copper complexes showed stronger antifungal activities than free ligands. The minimum inhibitory concentrations (MIC) of the copper complexes were found in the range of $8{\sim}28\;{\mu}g/ml$. These compounds represent a novel class of metal-based antifungal agents which provide opportunities for a large number of synthetic variations for modulation of the activities.

Microbe-Mediated Control of Mycotoxigenic Grain Fungi in Stored Rice with Focus on Aflatoxin Biodegradation and Biosynthesis Inhibition

  • Mannaa, Mohamed;Kim, Ki Deok
    • Mycobiology
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    • v.44 no.2
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    • pp.67-78
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    • 2016
  • Rice contaminated with fungal species during storage is not only of poor quality and low economic value, but may also have harmful effects on human and animal health. The predominant fungal species isolated from rice grains during storage belong to the genera Aspergillus and Penicillium. Some of these fungal species produce mycotoxins; they are responsible for adverse health effects in humans and animals, particularly Aspergillus flavus, which produces the extremely carcinogenic aflatoxins. Not surprisingly, there have been numerous attempts to devise safety procedure for the control of such harmful fungi and production of mycotoxins, including aflatoxins. This review provides information about fungal and mycotoxin contamination of stored rice grains, and microbe-based (biological) strategies to control grain fungi and mycotoxins. The latter will include information regarding attempts undertaken for mycotoxin (especially aflatoxin) bio-detoxification and microbial interference with the aflatoxin-biosynthetic pathway in the toxin-producing fungi.

Mycoflora and Mycotoxins of Cereal Grains in Delta, Egypt

  • Soliman, Hoda M.
    • Mycobiology
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    • v.31 no.4
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    • pp.183-190
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    • 2003
  • Five cereal grains(wheat, barley, rice, maize and sorghum) were collected from three Egyptian provinces known to be grain producers(Daqahlia, Gharbia and Kafer el-Sheikh). Two species of Alternaria(A. raphani and A. tenusinae); two species of Aspergillus(A. flavus and A. niger); one species of Cunninghamella(C. elegans); one Dreschslera species(D. myakt); three Fusarium species(F. graminearum, F. moniliform and F. solani); one Rhizopus species(R. stolonifer) and two species of Penicillium(P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. All the fungal isolates were tested for the production of toxic metabolites in culture media and the percentages of toxigenic isolates were calculated. The biological assay of the toxigenic fungal isolates showed significant variations in toxigenic activity. Thin layer chromatography revealed that the most active isolate produces moniliformin in culture media. The effect of culture conditions on the production of moniliformin was studied.