• Journal of Nutrition and Health
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• 제19권4호
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• pp.246-254
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• 1986

이유한 Sprague Dawley 종의 암컷 흰쥐를 두군으로 나누어 각각 pyridoxine이 충분한 식이 (22mg/kg diet)와 pyridoxine이 부족한 식이 (1.2mg/kg diet0로 성장시킨후, 적절한 시기에 임신시켰다. 태어난 새끼가 이유할 때 결핍식이군을 둘로 나누어, 한쪽은 pyridoxine이 충분한 식이로 바꾸어준후, 세군의 쥐를 생후 10-주까지 성장시켰다. 각 군의 새끼쥐가 각각 0, 3, 7, 10 주 되었을대, 간의 mitochondria 및 cytosolic fraction 에 있는 Asparate aminotransferase [ EC 2.6.1.1] activity 및 pyridoxal phosphate의 함량을 측정하였다. 결핍군의 간 aspartate aminotransterase activity는 mitochondria 및 cytosolic fraction에서 각각 대조군에 비해 유의적으로 낮았으며 10-4 M pyridoxal phosphate를 첨가한 후 측정한 total enzyme activity 는 거의 대조군과 비슷하였다. 3 주이후 pyridoxine 이 충분한 식이로 바구어준 회복군에서도 대조군에 비해 유의적으로 낮았으나, 결핍군보다는 높았다. 이두 fraction 에서의 pyridoxal phosphate 함량은 결핍군이 대조군보다 현저히 낮았으며, 회복군은 대조군과 비슷하였다. 이로써, 장기간에 걸친 pyridoxine결핍이간의 aspartate aminotransferase activity 및 pyridoxal phosphate에 현저한 영향을 미치며 이유기 이후의 식이보충에 의해서도 enzyme activity는 쉽게 회복되지 않음을 알 수있다.

• 한국식품저장유통학회지
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• 제18권3호
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• pp.407-413
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• 2011

허브의 일종인 B. sarmienti를 이용하여 숙취 해소 음료를 개발하고 그에 따른 알콜 대사 및 항산화 효소에 미치는 영향을 알아보고자 하였다. 알코올을 섭취한 쥐에 B. sarmienti 음료군(BSP)을 투여 한 경우 타사 제품군인 PC 그리고 B. sarmienti만을 제외한 음료군인 NBS보다 더 뛰어난 알코올 분해능 및 ADH, ALDH에서도 뛰어난 활성을 보였다. 또한 생화학적 검사를 통해 안전성을 확인하였으며, ALP, ALT 그리고 AST에서도 PC군에 비해 유의적인 감소 효과를 보였다. 항산화 활성 및 효소계에 있어서도 BSP군에서는 GSH에서는 CO군에 비해 효소의 함량은 높으나, 다른 군에 비해 유의적인 차이는 보이지 않았으며, TBARS에는 다른 군에 비해 함량이 감소됨을 알 수 있었으나 뚜렷한 차이는 보이지 않았다. 그러나 GST, CAT 그리고 SOD 효소에 있어서는 CO군에 비해 유의적인 효소 활성이 감소되었음을 알 수 있었다. 이상과 같은 결과를 토대로 B. sarmienti 추출물이 첨가된 음료는 뛰어난 숙취 해소 및 간세포의 손상을 보호하는 음료로서 이용 가능하리라 기대 된다.

• 한국자원식물학회지
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• 제17권3호
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• pp.283-288
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• 2004

시호내에 존재하는 간장기능증진에 효과적인 기능성 물질의 소재를 탐색하기 위한 기초연구의 일차실험으로 $CCl_4$에 의해 간장을 손상시킨 흰쥐에게 MeOH 시호추출물을 수준을 달리 하여 급여한 후 간 손상 치유 및 기능회복에 미치는 영향을 처리군 간에 비교, 검토했다. 그 결과, 혈중 AFP및 Total protein량은 시호추출물처리에 의해 감소했다 그러나 혈중 Albumin의 농도는 일정한 경향을 보여주지 않았다. 혈중 AST와 ALT의 활성치는 시호처 리 군에서 하락하였다. 혈중 ALP와 LDH의 활성치는 시호첨가군에서 감소했다 그러나 ${\gamma}$-G7의 활성치는 처리군간에 일정한 경향을 나타내지 않았다. 혈중 Total cholesterol과 Triglyceride량은 전 처리 군에서 유의한 차이를 보여 주지 않았다.

• 한국약용작물학회지
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• 제7권2호
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• pp.107-114
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• 1999

미나리는 간을 보호하며 간질환을 예방, 치유하는 효능이 있는 기능성식물로 인식되어 민간요법에 널리애용되고 있다. 그래서 알콜을 렛트와 마우스에 처리하여 만성, 아급성, 급성중독을 유도하고 밭미나리 발효액과 추출물 그리고 물미나리추출물의 효과를 조사한 결과는 다음과 같다. 렛트와 마우스에 에탄올을 2일동안 투여한결과 total cholesterol과 total glyceride의 수치가 증가되었으며 20일동안 밭미나리발효액과 추출물 그리고 물미나리추출물을 투여한후 에탄올을 2일동안 급여한균에서는 total cholesterol과 total glyceride의 감소 효과가 있었으나 처리간의 유의성은 인정되지않았다. 그러나 실험동물의 혈청중에 알콜에의해서 높아졌던 alanine aminotransferase 와 asparate aminotransferase는 밭미나리발효액 과 추출물에 의해서 감소되었으며 밭미나리발효액투여군에서 가장 많이 감소되어 처리간에 유의성이 인정되었다. 렛트에 10%에탄올을 10개월간 투여하여 만성알콜중독을 유발시킨다음 5일동안 물만 급여한군과 밭미나리발효액을 투여한군을 비교한바 total cholesterol은 40%, total glyceride는 60%의 감소효과가 있었으며 alanine aminotransferase 와 asparate aminotrasferase는 87.2%와 91.7%를 감소시켜 정상에 가깝게 회복시키는효과가 인정되었다. alkaline phosphatase, catalase, superoxide dismutase 및 glutathione peroxidase의 활성은 알콜의 처리에 의해서 급만성모두 유의적인 변동을 나타냈으며 밭미나리 발효액의 처리에 의해서 alkaline phosphatase와 superoxide dismutase의 활성에 특히 유의적인 감소를 가져왔다. 이와같은 결과는 물미나리보다 밭미나리발효액이 알콜성 간질환에 효능이있음을 시 사하는것이라 하겠다.

• 대한수의학회지
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• 제34권3호
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• pp.635-645
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• 1994

The environmental pollution by heavy metals has become a significant hygenic problem. Recently it is said the garlic supplementation in diet have protective effect on the heavy metal accumulation in tissue of rat by a researcher. In this study two experiments were undertaken. Firstly, dogs were fed diet supplemented with cadmium of cadmium plus 7% of raw garlic for 50 days. on the 50th day of experiment all dogs were euthanasied and the cadmium content of various tissue were measured by atomic absorption spectrophotometeric method. Secondly, in order to find out the toxic effect of garlic dogs were fed diet supplemented with 7% of raw garlic for 50 days and hematological or hematochemical observations were undertaken at 10 day intervals. The results obtained from these experiments were summarized as follows; 1. In dogs treated with cadmium and cadmium plus garlic cadmium was accumulated in renal cortex, liver, and muscle. However the concentrations of cadmium showed no significant between the two group. 2. In dogs fed diets supplemented with 7% of raw garlic significant decrease of erythrocyte count, packed cell volume and hemoglobin content and significant increase of Heinz body erythrocyte and reticulocyte count were recognized. 3. The garlic-treated dogs and control dogs had normal asparate aminotransferase, alanine aminotransferase, alkaline phosphotase, blood urea nitrogen value. The value showed no significant difference between the two group. 4. From these results it may be concluded that the garlic have protective effect for cadmium accmulation in tissue and excess garlic in diet could induce hemolytic anemia in dogs.

• 동의생리병리학회지
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• 제18권2호
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• pp.413-418
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• 2004

Objectives : Cirsii Japonici Herba (CJH) is one of medicinal plants that has been frequently used for styptic purposes in Asian countries. In order to evaluate a hepatoprotective effects of CJH in the liver fibrotic diseases, the present study investigated how CJH improves a hepatic function in the dimethylnitrosamine(DMN) treated rat. Methods : CJH were orally administered to rats that has been treated with DMN. Subsequently, the amount of blood L-asparate aminotransferase (AST), L-alanine aminotransferase (ALT), and hydroxyproline were quantitated. Several histopathological markers for examining the degree of hepatic fibrosis were investigated by H-E and Masson-Trichrome staining. Results: DMN treatment caused a increase of relative liver weight to the body at 14 days after DMN induction, Administration of CJH with 100mg/kg and 1,000mg/kg dose decreased significantly the AST level elevated by DMN injection(p<0.01). But ALT level was not improved. The hydroxyproline level was reduced by a simultaneous treatment of CJH with DMN for 7 days, but not recovered completely to its normal value, CJH administration improved conspicuously the DMN-induced histopathological changes of liver such as granuloma, but cell necrosis and fibrosis were not improved with CJH 1,000mg/kg dose. Conclusion: These results indicate that CJH has protective effect on liver injury and can inhibit liver fibrosis Induced by DMN in rats.

• Journal of Nutrition and Health
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• 제40권1호
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• pp.5-13
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• 2007

This study was designed to examine the effects of Salicornia herbacea L. (glasswort: GW) on the plasma blood glucose and lipid metabolites in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats weighing 200-220g by an injection of streptozotocin (STZ) dissolved in a citrate buffer into the tail vein at a dose of 45 mg/kg of body weight. Sprague-Dawley rats were fed an AIN-93 recommended diet and the experimental groups were fed a modified diet containing 10% and 20% of glasswort powder for 4 weeks. The experimental groups were divided into 6 groups which consisted of normal (N)-control group, N-GW 10% and N-GW 20% treated groups, STZ-control, STZ-GW 10% and STZ-GW 20% treated groups. The rats' body weights, aminotransferase activities and hematocrit (Hct) values were measured, along with plasma levels of glucose, protein, cholesterol, HDL-cholesterol, triglyceride (TG) and free fatty acids (FFA). The non-diabetic rats gained weight, while the diabetic rats lost weight. There were significant differences between the control group and the diabetic groups in the weight of the kidney, liver and pancreas. Asparate aminotransferase activity was lower in the non-diabetic control group compared to diabetic experimental groups, even though the difference was not significant. The plasma protein of N-GW 20% group was lower among all experimental groups but it was not significantly different. The blood glucose levels of the STZ-GW 10% group and STZ-GW 20% group were significantly lower than for the diabetic-control group. There were no significant difference of cholesterol levels among diabetic groups. The normal rats of 20% glasswort group in FFA and TG levels showed significant changes among all groups. These results exhibited dose related effect of glasswort and it may contain antihypoglycemic compounds.

• 한방재활의학과학회지
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• 제30권2호
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• pp.37-46
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• 2020

Objectives In this study, we analyzed the effect of Dioscoreae Rhizoma on exercise practice ability and blood fatigue variable factor in the forced swimming test. Methods Male mice were divided into four group. Mice were administrated with saline, Discorae Rhizoma extracted by water (DRW), Discorae Rhizoma extracted by 30% ethanol (DRE), red ginseng (RG), orally once per day, for 1 week. Exercise performance was evaluated by exhaustive swimming time and weights. Glycogen contents of liver and soleus muscle were measured at the end of the experiment. In addition, the biochemical parameters such as asparate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen, creatine, lactate dehydrogenase (LDH), ammonia, triglycerides (TG), total cholesterol (TC) and free fatty acid (FFA) were analyzed. Results Both DRW and DRE showed an improvement of swimming endurance compared with control group. Levels of ALT, ammonia, TG, TC and FFA were significantly decreased in both DRW and DRE compared with control group. Levels of AST, Creatine were significantly reduced in DRE group compared with control group. Moreover LDH showed only a tendency to increase in the DRW and DRE groups. Conclusions In summary, the present study showed that Dioscoreae Rhizoma improved in physical fatigue and exercise practice ability and DRE improved the superior than those of DRW.

• Toxicological Research
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• 제25권1호
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• pp.29-33
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• 2009

Phellinus gilvus (PG) is a widely used mushroom for health promotion. We studied the hepatoprotective effect of the polysaccharide aqueous extract of PG (PGP) against carbon tetrachloride ($CCl_4$)-induced liver injury in rats. Sprague Dawley rats were divided into 5 groups: Normal control, $CCl_4$ control, PGP 50, 100, and 200 mg/kg + $CCl_4$. The levels of serum biochemical parameters, liver lipid peroxide and antioxidant enzymes, and histological appearances were evaluated. The $CCl_4$-induced increments of alanine aminotransferase, asparate aminotransferase, and alkaline phosphatase levels in serum were significantly decreased by PGP-pretreatments. The PGP dose-dependently decreased hepatic malondialdehyde formations in $CCl_4$-treatment groups. Hepatic antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) were elevated by PGP in $CCl_4$-treatment groups. Histopathological evaluation of liver showed that the loss of hepatocytes, fatty changes, swelling and extensive necrosis of hepatocytes in centrilobular regions of the $CCl_4$-treated rats were ameliorated by PGP pretreatment. The PGP has hepatoprotective and antioxidative effects in $CCl_4$-induced liver injury of rat.

• Korean Journal of Acupuncture
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• 제38권4호
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• pp.275-281
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• 2021

Objectives : This study reports changes in liver function test (LFT) after Korean Medicine treatment in patients admitted to Korean Medicine hospital with abnormal LFT. The purpose of this study is to investigate the relationship between the Korean medicine treatment and abonormal LFT to verify safety of Korean medicine treatment by analyzing index of LFT. Methods : From Oct. 2015 to Sep. 2020, the result was analyzed for 91 patients admitted to the Pohang Korean Medicine Hospital and received Korean Medicine treatment. Asparate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (TB) were compared at admission and discharge. Results : Comparison between admission and discharge LFT showend AST decreased from 52.72±25.08 to 43.2±19.20, ALT from 70.85±32.40 to 62.13±29.40, and TB from 1.33±0.37 to 0.81±0.29. Conclusions : After Korean medicine treatment, AST, ALT, and TB decreased compared with the values at admission. Further studies on safety of Korean Medicine treatment are warranted.